scholarly journals Development of Salt- and Gastric-Resistant Whey Protein Isolate Stabilized Emulsions in the Presence of Cinnamaldehyde and Application in Salad Dressing

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1868
Author(s):  
Huanhuan Cui ◽  
Qihang Liu ◽  
David Julian McClements ◽  
Bin Li ◽  
Shilin Liu ◽  
...  
Keyword(s):  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Counter Ions ◽  
Monovalent Ions ◽  
Wide Range ◽  
Gastric Fluids ◽  
The Impact ◽  
Droplet Aggregation ◽  
Salt Stability

Protein-stabilized emulsions tend to be susceptible to droplet aggregation in the presence of high ionic strengths or when exposed to acidic gastric conditions due to a reduction of the electrostatic repulsion between the protein-coated droplets. Previously, we found that incorporating cinnamaldehyde into the oil phase improved the resistance of whey protein isolate (WPI)-stabilized emulsions against aggregation induced by NaCl, KCl and CaCl2. In the current study, we aimed to establish the impact of cinnamaldehyde on the tolerance of WPI-stabilized emulsions to high salt levels during food processing and to gastric conditions. In the absence of cinnamaldehyde, the addition of high levels of monovalent ions (NaCl and KCl) to WPI-emulsions cause appreciable droplet aggregation, with the particle sizes increasing from 150 nm to 413 nm and 906 nm in the presence of NaCl and KCl, respectively. In contrast, in the presence of 30% cinnamaldehyde in the oil phase, the WPI-emulsions remained stable to aggregation and the particle size of emulsions kept within 200 nm over a wide range of salt concentrations (0–2000 mM). Divalent counter-ions promoted droplet aggregation at lower concentrations (≤20 mM) than monovalent ones, which was attributed to ion-binding and ion-bridging effects, but the salt stability of the WPI emulsions was still improved after cinnamaldehyde addition. The incorporation of cinnamaldehyde into the oil phase also improved the resistance of the WPI-coated oil droplets to aggregation in simulated gastric fluids (pH 3.1–3.3). This study provides a novel way of improving the resistance of whey-protein-stabilized emulsions to aggregation at high ionic strengths or under gastric conditions.

Morphological and physical properties of kefiran-whey protein isolate bionanocomposite films reinforced with Al2O3 nanoparticles

2020 ◽  
Vol 26 (8) ◽  
pp. 666-675
Author(s):  
Zahra Moradi
Keyword(s):  
Mechanical Properties ◽  
Whey Protein ◽  
Water Solubility ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Alumina Nanoparticles ◽  
Vapor Permeability ◽  
Al2o3 Nanoparticles ◽  
Bionanocomposite Films ◽  
The Impact

Considering environmental pollution caused by the non-biodegradable polymers used in food packaging, developing and enhancing the properties of biodegradable films seem to be necessary. For this aim, in the present study, kefiran-whey protein isolate bionanocomposite films were prepared and the impact of different concentrations (1, 3 and 5% w/w) of Al2O3 (alumina) nanoparticles on their physical, morphological, thermal and mechanical properties was studied. Based on the obtained results, an increase in the nanoparticles content led to a significant decrease (p < 0.05) in the water vapor permeability, moisture absorption, moisture content, and water solubility. Scanning electron microscope images showed a homogeneous structure, confirming the good dispersion of alumina nanoparticles with smooth surface up to concentration of 3%. In addition, both thermal stability and mechanical properties of the films were improved by the increased concentrations of alumina. The results of X-ray diffraction indicated that the intensity of the crystalline peaks of film increased with the addition of Al2O3 to kefiran-whey protein isolate matrix. By considering all results, the concentration of 3% was proposed as the appropriate concentration of Al2O3 for the nano-reinforcement of kefiran-whey protein isolate bionanocomposites.

scholarly journals The Effect of Atmospheric Plasma on the Solubility and Dispersibility of Powdered Whey Protein Isolate

2018 ◽  
Author(s):  
Heidi Lightfoot
Keyword(s):  
Plasma Treatment ◽  
Whey Protein ◽  
Functional Properties ◽  
Protein Solubility ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Atmospheric Plasma ◽  
Pristine Sample ◽  
Food And Nutrition ◽  
The Impact

Functional properties of protein macromolecules such as protein solubility are of particular interest to the food and nutrition industries as they have significant implications on other useful properties and characteristics for the development of nutritional and food supplements. Consequently, proteins with specific and consistent functional characteristics are in high demand as essential ingredients in formulated food or in pharmaceutical and industrial mixtures. Proteins need to be highly soluble so that their functional properties can be effectively exploited, therefore methods to improve the solubility of protein powders are currently being developed. It has been hypothesized that atmospheric plasma treatment has an effect on protein solubility and dispersibility. This theory has not been yet explored with whey protein isolate elsewhere; this study is the first to explore the impact of plasma based treatment. The effect of atmospheric plasma treatment on the solubility and dispersibility of dry protein powder has been studied. Each variable was examined using both a pristine sample of whey protein isolate and a sample of whey protein isolate from the same product batch that had been exposed to atmospheric plasma (following ISO 8156 and ISO/TS 17758 protocols). We demonstrate that plasma can successfully increase the solubility and dispersibility of whey protein powder.

The influence of flaxseed gum on the microrheological properties and physicochemical stability of whey protein stabilized β-carotene emulsions

2017 ◽  
Vol 8 (1) ◽  
pp. 415-423 ◽  
Author(s):  
Duoxia Xu ◽  
Yameng Qi ◽  
Xu Wang ◽  
Xin Li ◽  
Shaojia Wang ◽  
...  
Keyword(s):  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Physicochemical Stability ◽  
The Impact ◽  
Β Carotene ◽  
Flaxseed Gum

The impact of flaxseed gum (FG) on the microrheological properties and physicochemical stability of whey protein isolate (WPI) stabilized β-carotene emulsions at pH 3.0 was studied.

scholarly journals Comparative Study of Oxidative Structural Modifications of Unadsorbed and Adsorbed Proteins in Whey Protein Isolate-Stabilized Oil-in-Water Emulsions under the Stress of Primary and Secondary Lipid Oxidation Products

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 593
Author(s):  
Jiaxin Chen ◽  
Jinhai Zhao ◽  
Baohua Kong ◽  
Qian Chen ◽  
Qian Liu ◽  
...  
Keyword(s):  
Lipid Oxidation ◽  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Dependent Manner ◽  
Structural Modifications ◽  
Oil In Water ◽  
Oxidative Modifications ◽  
Adsorbed Proteins ◽  
The Impact

The impact of typical primary or secondary lipid oxidation (LPO) products, selected as linoleic acid 13-hydroperoxide (13-HPODE) and malondialdehyde (MDA), on the structural modification of unadsorbed or adsorbed proteins in whey protein isolate (WPI)-stabilized oil-in-water (O/W) emulsions during storage up to 48 h at 37 °C in the dark was investigated. The results showed that either 13-HPODE and MDA could lead to structural modifications of unadsorbed or adsorbed proteins with a concentration-dependent manner and time relationship, respectively. Moreover, higher levels of MDA rendered a higher degree of oxidative modifications of WPI than 13-HPODE, indicated by the higher protein carbonyl contents and N’-formyl-L-kynurenine (NFK) and lower fluorescence intensity. Additionally, adsorbed proteins were more easily oxidized by LPO products than unadsorbed proteins. Overall, our results indicated that the formation of secondary LPO products and the protein position were crucial factors to increase the degree of oxidative modifications of WPI in O/W emulsion systems.

scholarly journals The Impact of Supplementation With Whey Protein Isolate and/or Omega-3 Fatty Acids on Sleep and Mood in Postmenopausal Women (SHAPE Study)

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 499-499
Author(s):  
Aubree Hawley ◽  
Caroline Baughn ◽  
Angela Tacinelli ◽  
Sam Walker ◽  
Xinya Liang ◽  
...  
Keyword(s):  
Sleep Quality ◽  
Postmenopausal Women ◽  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Mood States ◽  
Sleep Disruption ◽  
Omega 3 ◽  
Wrist Actigraphy ◽  
The Impact

Abstract Objectives Approximately 30% of adults ≥ 50 years of age suffer from poor sleep quality and the prevalence of sleep disruption is notably higher in postmenopausal women, with 35 to 60% reporting significant sleep disruption.  Sleep deprivation and low sleep quality are associated with increased energy intake, insulin resistance, elevated glucose, and mood disturbances (e.g., stress, cortisol, and depression). The objective of this study was to determine if the effect of protein and/or omega-3 fatty acid (O3FA) supplementation improves mood and sleep in postmenopausal women. Methods Thirty-nine postmenopausal women (age: 61.3 ± 8.7 years; BMI: 27.6 ± 6.6 kg/m2) were randomly allocated to one of 5 groups: 1) control (CON; no intervention free-living; n = 6), 2) whey protein isolate (PRO; 25 g/d; n = 7), 3) O3FA (DHA/EPA; 4.3 g/d; n = 10), 4) PRO + placebo soybean oil (PRO + PLA; 4.1 g/d; n = 7), or 5) PRO + O3FA (n = 9). Sleep and mood states were assessed at 0, 4, 8, 12, and 16 weeks, except objective sleep, which was assessed at 0, 8, and 16 weeks.  Sleep was measured using the Pittsburgh Sleep quality Index (PSQI) and wrist actigraphy, mood was measured using the Profile of Mood States (POMS), and markers of sleep and mood, orexin, BDNF, and cortisol, were measured using commercially available kits. Results A significant time effect (P &lt; 0.05) was observed on subjective sleep (PSQI), subjective mood states, BDNF, and cortisol concentrations. Although not significant, a greater % decrease in PSQI Global Sleep Score was observed in PRO (-30.3%), O3FA (-23.3%), PRO + PLA (−20.2%), and PRO + O3FA (−26.4%) when compared to an increase in CON (+18%). Similarly, a decrease trend in POMS Total Mood Disturbances from baseline was observed in PRO (−10 ± 27.7), O3FA (−6.7 ± 18.2), and PRO + O3FA (−6.9 ± 27.7) compared to no change in PRO + PLA (0.1 ± 11.5) and an increase in CON (5 ± 15.8). We observed a significant treatment effect on orexin (OXA; pg/mL; P &lt; 0.05). OXA increased significantly in PRO + O3FA compared to all other groups (P &lt; 0.05). Conclusions Although not significant, the data suggests individual and combined supplementation of protein and O3FA have the potential to improve sleep, mood, and orexin levels in postmenopausal women. NCT0303041 Funding Sources Arkansas Biosciences Institute

The impact of the molecular weight of dextran on formation of whey protein isolate (WPI)–dextran conjugates in fibers produced by needleless electrospinning after annealing

2018 ◽  
Vol 9 (4) ◽  
pp. 2193-2200 ◽  
Author(s):  
Deniz Turan ◽  
Monika Gibis ◽  
Gurbuz Gunes ◽  
Stefan K. Baier ◽  
Jochen Weiss
Keyword(s):  
Molecular Weight ◽  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Electrospun Fibers ◽  
Molecular Weights ◽  
Mixing Ratios ◽  
Needleless Electrospinning ◽  
The Impact

The conjugation reaction of electrospun fibers of a mixture of whey protein isolate (WPI) and dextran using different molecular weights (40, 70, and 100 kDa) and mixing ratios was studied.

scholarly journals Functional and Technological Potential of Whey Protein Isolate in Production of Milk Beverages Fermented by New Strains of Lactobacillus helveticus

2020 ◽  
Vol 10 (20) ◽  
pp. 7089
Author(s):  
Katarzyna Skrzypczak ◽  
Waldemar Gustaw ◽  
Emilia Fornal ◽  
Anna Kononiuk ◽  
Monika Michalak-Majewska ◽  
...  
Keyword(s):  
Whey Protein ◽  
Water Solution ◽  
Fermented Milk ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Lactobacillus Helveticus ◽  
Biologically Active ◽  
Protein Nitrogen ◽  
Technological Potential ◽  
Wide Range

With their desired functional and technological properties, whey protein preparations are used in the food industry. In turn, lactic acid fermentation may contribute to release of a wide range of biologically active peptides (BAPs) (known also as bioactive peptides or biopeptides) from whey and milk proteins, which are perceived as potential therapeutic tools and important constituents of personalized food suitable for prevention of many civilization and diet-related diseases. Therefore, the objective of this study was to determine the suitability of new Lb. helveticus strains for production of fermented milk beverages (drinking type) supplemented with whey protein isolate (WPI). Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) was employed to assess if WPI (water solution) might be a suitable precursor for BAPs produced by selected strains of Lb. helveticus. In order to identify the bioactivities of the peptides generated in WPI hydrolysates, the procedures indicated in databases were used. The fermented products differed from each other in some texture parameters, the content of protein, total nitrogen, and non-protein nitrogen, and the proteolysis index, which was dependent on the strain. Strain B734 was found to exhibit technological potential for development of new health-oriented fermented milk beverages with characteristics of functional food. Additionally, it proved to be able to release a wide range of BAPs from WPI with antioxidative, antibacterial, and immuno- and cyto-modulatory effects, as well as ACE (angiotensin-converting enzyme) inhibitory and antihypertensive activities.

scholarly journals Surface Hydrophobicity and Functional Properties of Citric Acid Cross-Linked Whey Protein Isolate: The Impact of pH and Concentration of Citric Acid

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2383 ◽  
Author(s):  
Tong Li ◽  
Chunyan Wang ◽  
Tianqi Li ◽  
Ling Ma ◽  
Dongxue Sun ◽  
...  
Keyword(s):  
Citric Acid ◽  
Whey Protein ◽  
Whey Proteins ◽  
Whey Protein Isolate ◽  
Surface Hydrophobicity ◽  
Protein Isolate ◽  
Cross Linking ◽  
Foaming Properties ◽  
Aqueous Conditions ◽  
The Impact

The effects of citric acid-mediated cross-linking under non-acidic conditions on the surface hydrophobicity, solubility, emulsifying, and foaming properties of whey protein isolate (WPI) were investigated. In this research, citric acid-mediated cross-linking could not only increase the surface hydrophobicity of whey proteins at pH 7.0 and 8.0, but it also improved its emulsifying and foaming properties. The emulsifying activity and foaming ability of WPI reached a maximum under the condition of 1% citric acid and pH 7.0. However, the solubility of WPI-CA gradually decreased with pH and the content of citric acid increased. Therefore, the cross-linking mediated by citric acid under non-acidic aqueous conditions, markedly altered the surface hydrophobicity and enhanced emulsifying and foaming properties of WPI.

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