scholarly journals A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae Porphyridium purpureum

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2164
Author(s):  
Tao Li ◽  
Jin Xu ◽  
Weinan Wang ◽  
Zishuo Chen ◽  
Chulin Li ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Red Algae ◽  
Uronic Acid ◽  
Cold Water ◽  
Extraction Process ◽  
Hot Water ◽  
Porphyridium Purpureum ◽  
Crude Lipid ◽  
Fluorescence Characteristics

The microalga Porphyridium accumulates high-value compounds such as phycoerythrin, polyunsaturated fatty acids, and polysaccharides, and thus, the extraction of these compounds could significantly expand the value of Porphyridium biomass. In the present study, a novel fractional extraction strategy based on the characteristics of these compounds was established using cold water, 95% ethanol, and hot water. The yield of phycoerythrin, lipids, and polysaccharides was 63.3, 74.3, and 75.2%, respectively. The phycoerythrin exhibited excellent fluorescence characteristics but had low purity. The crude lipid was dark with poor fluidity. Digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerol containing C20:5 and C20:4 were the most abundant glycerolipids, while glucose, xylose, and galactose constituted the intracellular polysaccharides that had covalently bound to proteins (8.01%), uronic acid (4.13%), and sulfate (8.31%). Compared with polysaccharides and crude lipids, crude phycoerythrin showed the best antioxidant activity. Overall, the three-step fractional extraction process was feasible for Porphyridium; however, further purification is necessary for downstream applications.

scholarly journals ATR-FTIR-based fingerprinting of some Cucurbitaceae extracts: a preliminary study

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Wirginia Kukula-Koch ◽  
Maciej Grzybek ◽  
Aneta Strachecka ◽  
Aleksandra Jaworska ◽  
Agnieszka Ludwiczuk
Keyword(s):  
Fatty Acids ◽  
Cucumis Melo ◽  
Cold Water ◽  
Saturated Fatty Acids ◽  
Principal Component ◽  
Ethanol Extract ◽  
Hot Water ◽  
Main Variable ◽  
Preliminary Study ◽  
Seed Extracts

The attenuated total reflectance-Fourier transform infrared (ATR-FTIR) fingerprinting of some selected cucurbits was performed on three types of seed extracts (alcoholic, cold-water, and hot-water) in order to elaborate a characteristic FTIR profile of their family representatives and to determine their biochemical content. Cluster analysis and principal component analysis were performed on <em>Cucumis melo</em> subsp. <em>melo</em> var. <em>inodorus</em>, <em>Cucurbita pepo</em>, <em>C. maxima</em>, <em>C. pepo</em> var. <em>cylindrica</em>, <em>C. maxima</em> subsp. <em>maxima</em> convar. <em>maxima</em> ‘Hokkaido’ to determine the similarities between their seed extracts. The ethanol extract of the <em>C. pepo</em> seeds was different from the other seed extracts because in addition to esters, it contained free fatty acids, which could influence its pharmacological activity. The main variable differentiating the extracts was the absorption band at 2,920–2,925 cm<sup>−1</sup>, which represented saturated fatty acids. The obtained results were analyzed by various statistical tools to evaluate the fingerprints of the selected species of cucurbits.

scholarly journals Composition of Brazil Nut (Bertholletia excels HBK), Its Beverage and By-Products: A Healthy Food and Potential Source of Ingredients

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3007
Author(s):  
Wilson V. Vasquez-Rojas ◽  
Diana Martín ◽  
Beatriz Miralles ◽  
Isidra Recio ◽  
Tiziana Fornari ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Unsaturated Fatty Acids ◽  
Water Soluble ◽  
Hot Water ◽  
Brazil Nut ◽  
Sediment Fraction ◽  
By Products ◽  
Lipid Components

The consumption of plant-based beverages is a growing trend and, consequently, the search for alternative plant sources, the improvement of beverage quality and the use of their by-products, acquire great interest. Thus, the purpose of this work was to characterize the composition (nutrients, phytochemicals and antioxidant activity) of the Brazil nut (BN), its whole beverage (WBM), water-soluble beverage (BM-S), and its by-products of the beverage production: cake, sediment fraction (BM-D), and fat fraction (BM-F). In this study, advanced methodologies for the analysis of the components were employed to assess HPLC-ESI-QTOF (phenolic compounds), GC (fatty acids), and MALDI-TOF/TOF (proteins and peptides). The production of WBM was based on a hot water extraction process, and the production of BM-S includes an additional centrifugation step. The BN showed an interesting nutritional quality and outstanding content of unsaturated fatty acids. The investigation found the following in the composition of the BN: phenolic compounds (mainly flavan-3-ols as Catechin (and glycosides or derivatives), Epicatechin (and glycosides or derivatives), Quercetin and Myricetin-3-O-rhamnoside, hydroxybenzoic acids as Gallic acid (and derivatives), 4-hydroxybenzoic acid, ellagic acid, Vanillic acid, p-Coumaric acid and Ferulic acid, bioactive minor lipid components (β-Sitosterol, γ-Tocopherol, α-Tocopherol and squalene), and a high level of selenium. In beverages, WBM had a higher lipid content than BM-S, a factor that influenced the energy characteristics and the content of bioactive minor lipid components. The level of phenolic compounds and selenium were outstanding in both beverages. Hydrothermal processing can promote some lipolysis, with an increase in free fatty acids and monoglycerides content. In by-products, the BM-F stood out due to its bioactive minor lipid components, the BM-D showed a highlight in protein and mineral contents, and the cake retained important nutrients and phytochemicals from the BN. In general, the BN and its beverages are healthy foods, and its by-products could be used to obtain healthy ingredients with appreciable biological activities (such as antioxidant activity).

scholarly journals Effect of the Yerba mate (Ilex paraguariensis) brewing method on the content of selected elements and antioxidant potential of infusions

2020 ◽  
Vol 22 (1) ◽  
pp. 54-60
Author(s):  
Katarzyna Janda ◽  
Karolina Jakubczyk ◽  
Agnieszka Łukomska ◽  
Irena Baranowska-Bosiacka ◽  
Ewa Rębacz-Maron ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cold Water ◽  
Hot Water ◽  
Biologically Active ◽  
Ilex Paraguariensis ◽  
Yerba Mate ◽  
High Antioxidant Activity ◽  
Place Of Origin ◽  
Brewing Method ◽  
Active Substances

AbstractYerba mate is a source of biologically active substances. The aim was to determine whether the place of origin of Yerba and the brewing method have any influence on the levels of Ca, Mg and Fe and antioxidant activity of infusions. Samples were steeped in cold water (25°C) and hot water (three consecutive infusions with 85°C water). Infusions had a high antioxidant activity and high Mg level. The levels of elements and the antioxidant activity were influenced by the brewing method. There were no significant differences in the examined parameters depending on the country of origin. The results on the levels of elements and the antioxidant activity indicate that the most efficient brewing method was infusion in hot water. The highest levels of elements were found in first infusions, with the highest antioxidant activity in the third infusions.

scholarly journals Pipes and Potions: Testing the Efficacy of European Folk Preparation Methods for Anticholinergic Solanaceae Plants

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 126
Author(s):  
Karsten Fatur ◽  
Matjaž Ravnikar ◽  
Vitjan Fras ◽  
Samo Kreft
Keyword(s):  
High Performance Liquid Chromatography ◽  
Plant Material ◽  
High Performance ◽  
Cold Water ◽  
Effective Means ◽  
Tropane Alkaloids ◽  
Extraction Process ◽  
Hot Water ◽  
Preparation Methods ◽  
Fortified Wines

The present article sought to evaluate the efficiency of various folk preparation methods commonly used in Europe for employing anticholinergic Solanaceae plants. The study aimed to uncover which folk methods were effective for the extraction of the anticholinergic tropane alkaloids of these plants, atropine and scopolamine. The folk extractions that were tested sought to simulate the preparation of teas, cold-water infusions, unguents, tinctures, fortified wines, and smoking. All preparation types and a control were then put through an extraction process to see what amount of the alkaloids had been maintained. These extractions were then analysed using high-performance liquid chromatography (HPLC). Cold- and hot-water preparations, tinctures, and fortified wines all proved to be effective means of extracting atropine and scopolamine from plant material under conditions seen in folk usage. Smoking and the oil-based unguent, however, yielded no alkaloids, suggesting a lack of efficiency for these preparations, a problem with our methodology, or possible chemical changes and losses associated with the preparation procedure.

CARBOHYDRATE PRODUCT FOR THERAPEUTIC AND PROPHYLACTIC PURPOSES

2020 ◽  
pp. 89-94
Author(s):  
Khvorova L.S. ◽  
Byzov V.A.
Keyword(s):  
Antioxidant Activity ◽  
Uric Acid ◽  
Phenolic Compounds ◽  
Mental Stress ◽  
The Body ◽  
Hot Water ◽  
Preventive Effect ◽  
Activity Inhibition ◽  
Medicinal Properties ◽  
Simple Carbohydrates

The article is devoted to the creation of a carbohydrate product with rosehip extract in the form of sweets (glucose Fudge) with a therapeutic and preventive effect aimed at restoring the body of people exposed to intense physical and mental stress and the effects of damaging environmental factors. As carbohydrates in the recipe, simple carbohydrates (glucose) and complex carbohydrates in the form of starch molasses containing (maltose, tri - Tetra - sugars, dextrins) are used. Glucose in the formulation is the main energy ingredient, sweetener and structure-forming component that gives the product a solid consistency due to crystallization. The medicinal effect of the product is provided by rosehip extract. Numerous published studies have established its vitamin, immunostimulating and antioxidant activity, inhibition of uric acid formation and obesity, which are associated with flavonoids, other phenolic compounds, and vitamins. The list of medicinal properties of rosehip extract shows the feasibility of using it in additional nutrition of people in order to prevent diseases. For the formulation of our sweets, the extraction of crushed rosehip fruits was carried out three times with hot water at a temperature of 65-700C, followed by concentration of the extract under vacuum to 25-30% SV. The resulting extract-concentrate was introduced into the formulation in a dosage of 5.5% SV per 100 g of SV glucose Fudge, which provided the necessary dosage for the product when used from 3 (for children) to 10 sweets a day (for adults) for the prevention of colds.

Experimental Investigation of Heat Transfer Enhancement by Using Different Number of Fins in Circular Tube

2018 ◽  
Vol 6 (3) ◽  
pp. 1-12
Author(s):  
Kamil Abdul Hussien
Keyword(s):  
Heat Transfer ◽  
Heat Exchanger ◽  
Cold Water ◽  
Finned Tube ◽  
Hot Water ◽  
Copper Tube ◽  
Smooth Tube ◽  
Tube Heat Exchanger ◽  
Mass Flow Rates ◽  
Flow Heat

Abstract-The present work investigates the enhancement of heat transfer by using different number of circular fins (8, 10, 12, 16, and 20) in double tube counter flow heat exchanger experimentally. The fins are made of copper with dimensions 66 mm OD, 22 mm ID and 1 mm thickness. Each fin has three of 14 mm diameter perforations located at 120o from each to another. The fins are fixed on a straight smooth copper tube of 1 m length, 19.9 mm ID and 22.2 mm OD. The tube is inserted inside the insulated PVC tube of 100 mm ID. The cold water is pumped around the finned copper tube, inside the PVC, at mass flow rates range (0.01019 - 0.0219) kg/s. The Reynold's number of hot water ranges (640 - 1921). The experiment results are obtained using six double tube heat exchanger (1 smooth tube and the other 5 are finned one). The results, illustrated that the heat transfer coefficient proportionally with the number of fin. The results also showed that the enhancement ratio of heat transfer for finned tube is higher than for smooth tube with (9.2, 10.2, 11.1, 12.1 13.1) times for number of fins (8, 10, 12, 16 and 20) respectively.

scholarly journals Optimization of banana crop by-products solvent extraction for the production of bioactive compounds

2021 ◽  
Author(s):  
Sara Díaz ◽  
Antonio N. Benítez ◽  
Sara Ramírez-Bolaños ◽  
Lidia Robaina ◽  
Zaida Ortega
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Ethanol ◽  
Ethanol Concentration ◽  
Extraction Process ◽  
Total Phenol Content ◽  
Optimal Conditions ◽  
Satisfactory Description ◽  
Optimized Conditions ◽  
By Products ◽  
Banana Crops

AbstractThe aim of this work is the optimization of phenolic compound extraction from three by-products of banana crops (rachis, discarded banana, and banana’s pseudostem pulp), as a way to valorize them through a green extraction process. The influence of the temperature and aqueous ethanol concentration (Et-OH) on extract properties (total phenol content (TPC) and antioxidant activity) was firstly analyzed. 78 ℃ and ethanol concentrations close to 50% yielded the best results for the three materials. The equations obtained by the response surface methodology gave a satisfactory description of the experimental data, allowing optimizing the extraction conditions. Under optimized conditions, time influence was then assessed, although this parameter seemed not influence results. Among the three by-products, rachis extract (60% Et-OH, 78 ℃, and 30 min) presented the highest TPC (796 mg gallic acid/100 g of dried material) and antioxidant activity (6.51 mg Trolox equivalents/g of dried material), followed by discarded banana, and pseudostem pulp. Under the optimal conditions, experiments were performed at a larger scale, allowing to determine the extraction yields (EY) and to characterize the extracts. The highest EY was obtained for the rachis (26%), but the extract with the highest activity was obtained for discarded banana (50% Et-OH, 78 ℃, and 60 min), which presented a TPC of 27.26 mg/g extract corresponding to 54.59 mg Trolox equivalents/g extract. This study contributes to the valorization of banana crops residues as a source of polyphenolic compounds with bioactive functions that can be extracted under economic extraction conditions. Graphical abstract

scholarly journals Antioxidant, Antimicrobial Activities and Fatty Acid Components of Flower, Leaf, Stem and Seed of Hypericum scabrum

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ali Shafaghat
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Hexane Extracts ◽  
Hypericum Scabrum ◽  
Different Parts

The hexane extracts of flower, leaf, stem, and seed of Hypericum scabrum, which were collected from northwestern Iran, were obtained by extraction in a Soxhlet apparatus. The fatty acids were converted to methyl esters and determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The hexane extract from the flower, leaf, stem, and seed contained 39.1%, 43.2%, 29.0%, and 37.6% of omega-3 fatty acids, respectively. The other main components of the flower extract were tetracosane (12.2%) and palmitic acid (9.3%), and that of the leaf extract was palmitic acid (7.4%). The stem and seed extracts contained bis(2-ethylhexyl)phthalate (18.7% and 35.7%), nonacosane (11.7% and 3.9%) and linoleic acid (6.5% and 6.9%) as major components. The hexane extracts of different parts from H. scabrum represent an important source of omega-3 fatty acids in several Hypericum species. The antioxidant activity of all hexane extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The results indicate that hexane extracts from different parts of H. scabrum possess considerable antioxidant activity. The highest radical scavenging activity was detected in seed, which had an IC50 = 165 μg/mL. The antimicrobial activity of the extracts of those samples were determined against seven Gram-positive and Gram-negative bacteria ( Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae), as well as three fungi ( Candida albicans, Saccharomyces cerevisiae, and Aspergillus niger). The bioassay showed that the oil exhibited moderate antimicrobial activity. This study reveals that the all parts of this plant are attractive sources of fatty acid components, especially the essential ones, as well as of effective natural antioxidants.

AMMONOLYSIS OF URONIC ESTER GROUPS IN BIRCH XYLAN

1964 ◽  
Vol 42 (11) ◽  
pp. 2434-2439 ◽  
Author(s):  
Paul Y. Wang ◽  
H. I. Bolker ◽  
C. B. Purves
Keyword(s):  
Alkaline Hydrolysis ◽  
Uronic Acid ◽  
Liquid Ammonia ◽  
Treated Wood ◽  
Hot Water ◽  
Amide Nitrogen ◽  
White Birch

Solvent-extracted white birch, kept near 20° in liquid ammonia under pressure for 72 h and then extracted with methanol to remove acetamide, retained 0.25% of amide nitrogen. Extraction of the very finely ground residual wood with hot water yielded 21% as a 4-O-methylglucuronoxylan containing 0.53% of amide nitrogen. Alkaline hydrolysis removed the amide absorption in the infrared and restored the normal xylan spectrum. These results, supported by various control experiments, showed that amide groups in the ammonia-treated wood were derived from lactone or ester groups in the original 4-O-methylglucuronoxylan. Some of the uronic acid groups in the native wood presumably existed as ester crosslinks.

Sign in / Sign up

Export Citation Format

Share Document