scholarly journals Thermal Processing of Peanut Grains Impairs Their Mimicked Gastrointestinal Digestion While Downstream Defatting Treatments Affect Digestomic Profiles

Foods ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 463 ◽  
Author(s):  
Ivana Prodić ◽  
Katarina Smiljanić ◽  
Ana Simović ◽  
Jelena Radosavljević ◽  
Tanja Ćirković Veličković
Keyword(s):  
Thermal Processing ◽  
Protein Extraction ◽  
Solid Food ◽  
Gastrointestinal Digestion ◽  
Intestinal Digestion ◽  
Delayed Extraction ◽  
Downstream Analysis ◽  
Peanut Proteins ◽  
Proteins And Peptides ◽  
Thermally Treated

Resistance to digestion by digestive proteases represents a critical property of many food allergens. Recently, a harmonized INFOGEST protocol was proposed for solid food digestion. The protocol proposes digestion conditions suitable for all kinds of solid and liquid foods. However, peanuts, as a lipid-rich food, represent a challenge for downstream analyses of the digestome. This is particularly reflected in the methodological difficulties in analyzing proteins and peptides in the presence of lipids. Therefore, the removal of the lipids seems to be a prerequisite for the downstream analysis of digestomes of lipid-rich foods. Here, we aimed to compare the digestomes of raw and thermally treated (boiled and roasted) peanuts, resulting from the INFOGEST digestion protocol for solid food, upon defatting the digests in two different manners. The most reproducible results of peanut digests were obtained in downstream analyses on TCA/acetone defatting. Unfortunately, defatting, even with an optimized TCA/acetone procedure, leads to the loss of proteins and peptides. The results of our study reveal that different thermal treatments of peanuts affect protein extraction and gastric/gastrointestinal digestion. Roasting of peanuts seems to enhance the extraction of proteins during intestinal digestion to a notable extent. The increased intestinal digestion is a consequence of the delayed extraction of thermally treated peanut proteins, which are poorly soluble in acidic gastric digestion juice but are easily extracted when the pH of the media is raised as in the subsequent intestinal phase of the digestion. Thermal processing of peanuts impaired the gastrointestinal digestion of the peanut proteins, especially in the case of roasted samples.

Nutritional Characteristics of Silkworm Pupae Protein En Peptides after Simulated Gastric and Simulated Intestinal Digestion In Vitro

2014 ◽  
Vol 1033-1034 ◽  
pp. 700-708
Author(s):  
Jia Jie Chen ◽  
Wen Hui Wu ◽  
Ling Ni ◽  
Ting Yan ◽  
Qing Bo Zhao ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nutritional Support ◽  
Gastrointestinal Digestion ◽  
Animal Feeds ◽  
Silkworm Pupae ◽  
Nutritional Characteristics ◽  
Intestinal Digestion ◽  
Proteins And Peptides

This study evaluated the nutritional characteristics and percentage of released nitrogen (%) of silkworm pupae proteins and peptides after simulated gastric and intestinal digestion, with a view to use in animal feeds or human nutritional support. Distribution of molecular mass and amino acids in the processed protein were analysed and the amino acid score, chemical score, and essential amino acid index (EAAI) calculated. Differential scanning calorimetry and simulated in vitro gastrointestinal digestion were used to analyse the denaturation temperature and absorption characteristics of samples. The denaturation temperatures suggested that silkworm pupae proteins and peptides were stable at room temperature. Silkworm pupae proteins and peptides contained all amino acids required by humans, and the composition was reasonable. Simulated gastrointestinal digestion resulted in about 90% nitrogen release amount. All data indicate that this protein may be useful as an economic, high quality supplement in animal feeds and for human nutritional support.

scholarly journals Optimization and Identification of Antioxidant Peptide from Underutilized Dunaliella salina Protein: Extraction, In Vitro Gastrointestinal Digestion, and Fractionation

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Enqin Xia ◽  
Lu Zhai ◽  
Zhigang Huang ◽  
Hairong Liang ◽  
Hui Yang ◽  
...  
Keyword(s):  
Dunaliella Salina ◽  
Protein Extraction ◽  
Amino Acid Sequences ◽  
Ultrasound Wave ◽  
Scavenging Activity ◽  
Gastrointestinal Digestion ◽  
Ultrasound Assisted Extraction ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

DPPH• scavenging peptides (<3kDa) from underutilized Dunaliella salina protein were obtained by the following successive treatment, i.e., ultrasound extraction, simulated in vitro gastrointestinal digestion hydrolyzation, and membrane ultrafiltration classification. The optimal condition for ultrasound-assisted extraction was an ultrasound wave with 800 W of power treating a mixture of 60 mL of 1.0 mol L−1 NaOH and 2 g algae powder for 15 min. A high correlation (r=0.8146) between DPPH• scavenging activity and yield of the intact peptides showed their antioxidant capacity. Simulated in vitro digestion assay resulted in excellent DPPH• scavenging activity of the total peptide, amounting to (86.5 ± 10.1)%, comparing with the nondigestion samples at (46.8 ± 6.5)%. After fractionation, the 500-1000 Da fraction exhibited the highest DPPH• scavenging activity (81.2 ± 4.0)%, increasing 1.5 times due to digestion. Then, the 500-1000 Da fraction was analyzed by RPLC-Q Exactive HF mass spectrometer, and 4 novel peptides, i.e., Ile-Leu-Thr-Lys-Ala-Ala-Ile-Glu-Gly-Lys, Ile-Ile-Tyr-Phe-Gln-Gly-Lys, Asn-Asp-Pro-Ser-Thr-Val-Lys, and Thr-Val-Arg-Pro-Pro-Gln-Arg, were identified. From these amino acid sequences, hydrophobic residues accounted for 56%, which indicated their high antioxidant property. The results indicated that underutilized protein of Dunaliella salina could be a potential source of antioxidative peptides through simulated in vitro gastrointestinal digestion.

Interactions between whey proteins and cranberry juice after thermal or non-thermal processing during in vitro gastrointestinal digestion

2020 ◽  
Vol 11 (9) ◽  
pp. 7661-7680
Author(s):  
Karen A. Rios-Villa ◽  
Mrittika Bhattacharya ◽  
Ellia H. La ◽  
Daniela Barile ◽  
Gail M. Bornhorst
Keyword(s):  
Whey Protein ◽  
Thermal Processing ◽  
Whey Proteins ◽  
Protein Digestibility ◽  
Cranberry Juice ◽  
Gastrointestinal Digestion ◽  
In Vitro Gastrointestinal Digestion

This study entails the possible interactions between whey protein and cranberry juice after processing, impacting either the protein digestibility or the bioaccessibility of cranberry antioxidants using an in vitro gastrointestinal digestion model.

scholarly journals Effect of In Vitro Digestion on the Antioxidant Compounds and Antioxidant Capacity of 12 Plum (Spondias purpurea L.) Ecotypes

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1995
Author(s):  
Xochitl Cruz Sollano-Mendieta ◽  
Ofelia Gabriela Meza-Márquez ◽  
Guillermo Osorio-Revilla ◽  
Darío Iker Téllez-Medina
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Digestive System ◽  
Total Content ◽  
In Vitro Digestion ◽  
Antioxidant Compounds ◽  
Gastrointestinal Digestion ◽  
Intestinal Digestion ◽  
Spondias Purpurea

Spondias purpurea L. plum is a source of antioxidant compounds. Nevertheless, once they are consumed and go through the digestive system, these compounds may undergo changes that modify their bioaccessibility. This study aimed to evaluate the effect of in vitro gastrointestinal digestion on the total content of carotenoids (TCC), ascorbic acid (AA), phenolic compounds (TPC), flavonoids (TFC), anthocyanins (TAC), and antioxidant capacity (ABTS, DPPH) of 12 plum Spondias purpurea L. ecotypes. The plum samples were subjected to the InfoGest in vitro digestion model. TCC, AA, TPC, TFC, TAC, ABTS, and DPPH were significantly different (p ≤ 0.05) in each in vitro digestion stage. The gastric stage released the highest content of AA (64.04–78.66%) and TAC (128.45–280.50%), whereas the intestinal stage released the highest content of TCC (11.31–34.20%), TPC (68.61–95.36%), and TFC (72.76–95.57%). Carotenoids were not identified in the gastric stage whilst anthocyanins were lost at the end of the intestinal digestion. At the gastric stage, AA presented a positive and high correlation with ABTS (r: 0.83) and DPPH (r: 0.84), while, in the intestinal stage, TPC and TFC presented positive and high correlation with ABTS (r ≥ 0.8) and DPPH (r ≥ 0.8), respectively.

scholarly journals The Influence of in Vitro Gastrointestinal Digestion of Brassica oleracea Florets on the Antioxidant Activity and Chlorophyll, Carotenoid and Phenolic Content

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 212 ◽  
Author(s):  
Teodora Scrob ◽  
Anamaria Hosu ◽  
Claudia Cimpoiu
Keyword(s):  
Antioxidant Activity ◽  
Kinetic Model ◽  
Brassica Oleracea ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Gastrointestinal Digestion ◽  
In Vitro Gastrointestinal Digestion ◽  
Intestinal Digestion

Brassica oleracea L. var. Italica is known to contain a wide variety of antioxidants and due to the protection against various diseases its consumption has been increasing over the years. Thus, knowledge of the changes that occur during the digestion process is of great interest. The aim of this study was to investigate the influence of in vitro gastrointestinal digestion of broccoli on antioxidant activity and on the chlorophyll, carotenoid and phenolic content. First, the ultrasound-assisted extraction of bioactive compounds was optimized and the kinetic model was evaluated. Then, the broccoli was subjected to a static simulated digestion. The antioxidant activity was monitored by ABTS [2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate)] assay and the contents of target compounds were investigated by UV-Vis spectrophotometry and thin-layer chromatography. The optimum conditions were: solvent—ethanol; time—20 min and temperature—30 °C, and a second order kinetic model was found to describe the mechanism of extraction. The antioxidant activity and carotenoid, chlorophyll and total phenolic content was significantly decreased after simulated gastric and intestinal digestion. The gastric digestion considerably decreased carotenoid and chlorophyll content, meanwhile the intestinal digestion significantly decreased the total phenolic content (TPC). The antioxidant activity was equally affected by both gastric and intestinal digestion.

Influence of Thermal Processing on the Allergenicity of Peanut Proteins

2005 ◽  
Vol 53 (11) ◽  
pp. 4547-4553 ◽  
Author(s):  
L. Mondoulet ◽  
E. Paty ◽  
M. F. Drumare ◽  
S. Ah-Leung ◽  
P. Scheinmann ◽  
...  
Keyword(s):  
Thermal Processing ◽  
Peanut Proteins

scholarly journals Mainly Dimers and Trimers of Chinese Bayberry Leaves Proanthocyanidins (BLPs) are Utilized by Gut Microbiota: In Vitro Digestion and Fermentation Coupled with Caco-2 Transportation

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 184
Author(s):  
Wenyang Tao ◽  
Chaoyang Wei ◽  
Shuyu Shen ◽  
Mengting Wang ◽  
Shiguo Chen ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Epigallocatechin Gallate ◽  
In Vitro Digestion ◽  
Gastrointestinal Digestion ◽  
Chinese Bayberry ◽  
Intestinal Digestion ◽  
Health Promoting

Chinese bayberry leaf proanthocyanidins (BLPs) are Epigallocatechin gallate (EGCG) oligomers or polymers, which have a lot of health-promoting activity. The activity is closely related to their behavior during in vitro digestion, which remains unknown and hinders further investigations. To clarify the changes of BLPs during gastrointestinal digestion, further research is required. For in vitro digestion, including gastric-intestinal digestion, colon fermentation was applied. Caco-2 monolayer transportation was also applied to investigate the behavior of different BLPs with different degrees of polymerization. The trimers and the tetramers were significantly decreased during in vitro gastric-intestinal digestion resulting in a significant increase in the content of dimers. The dimers and trimers were the main compounds utilized by gut microbiota and they were assumed not to degrade through cleavage of the inflavan bond. The monomers and dimers were able to transport through the Caco-2 monolayer at a rate of 10.45% and 6.4%, respectively.

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