scholarly journals Acid-Catalyzed Water Extraction of Two Polysaccharides from Artemisia argyi and Their Physicochemical Properties and Antioxidant Activities

Gels ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 5
Author(s):  
Yuan Ruan ◽  
Chaofei Niu ◽  
Pengzhan Zhang ◽  
Yanyan Qian ◽  
Xinxin Li ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extraction ◽  
Molar Ratio ◽  
Composition Analysis ◽  
Methylation Analysis ◽  
Artemisia Argyi ◽  
Acid Catalyzed ◽  
Radical Scavenging Assay

In this study, two purified polysaccharide fractions, Artp1 and Artp2, were obtained using acid-catalyzed water extraction, and then purified by DEAE-52 cellulose and Sephadex G-200 column chromatography from the crude polysaccharides of Artemisia argyi. Their physicochemical properties were investigated by gel permeation chromatography (GPC), high-performance anion exchange chromatography (HPAEC), Fourier transform infrared (FT-IR), scanning electron microscope (SEM), thermal analysis, and methylation analysis. The average molecular weight (Mw) of Artp1 and Artp2 were estimated to be 42.17 kDa and 175.22 kDa, respectively. Monosaccharide composition analysis revealed that the Rha, Gal, and GalA occupied main proportion in Artp1 with the molar ratio of 25.1:24.7:40.4, while the Rha, Gal, Xly, and GalA occupied the main proportion in Artp2 with the molar ratio of 16.7:13.5:12.8:38.7. Due to the high yield and the relatively high carbohydrate content, the Artp1 was determined by the methylation analysis and NMR. The results of Artp1 indicated that 1,4-GalpA and 1,2,4-Rhap formed the backbone with some 1,2-Rhap, 1,3-Galp, and 1,6-Galp in the backbone or the side chains. Artp1 and Artp2 exhibited effective antioxidant activities by DPPH radical scavenging assay and hydroxyl radical scavenging assay in a dose-dependent manner. These investigations of the polysaccharides from A. argyi. provide a scientific basis for the uses of Artp1 and Artp2 as ingredients in functional foods and medicines.

scholarly journals Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay

Data in Brief ◽  
2018 ◽  
Vol 17 ◽  
pp. 870-875 ◽  
Author(s):  
Kotaro Hara ◽  
Takao Someya ◽  
Katsura Sano ◽  
Yoshimasa Sagane ◽  
Toshihiro Watanabe ◽  
...  
Keyword(s):  
Free Radical ◽  
Sri Lanka ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

scholarly journals Optimized microwave reflux extraction and antioxidant activities of piperine from black and white piper nigrum

2017 ◽  
Vol 19 ◽  
pp. 139 ◽  
Author(s):  
Olalere Olusegun Abayomi ◽  
Abdurahman Hamid Nour ◽  
Alara Oluwaseun Ruth ◽  
Omar Abed Habeeb
Keyword(s):  
Particle Size ◽  
Chemical Engineering ◽  
Antioxidant Activities ◽  
Irradiation Time ◽  
Power Level ◽  
Radical Scavenging ◽  
Molar Ratio ◽  
Piper Nigrum ◽  
White Pepper ◽  
Black And White

<p>In this study, the microwave reflux technique was employed to extract piperine from black and white pepper. This is due to its ability to combine the microwave and the conventional solvent extraction together with a high degree of selectivity and quality extract. The extraction process was optimized using theL<sub>9</sub>-Taguchi experimental design. This investigated the effects of four independent factors (irradiation time (x<sub>1</sub>), microwave power level (x<sub>2</sub>), feed particle size (x<sub>3</sub>) and molar ratio (x<sub>4</sub>)) on piperine yield. Antioxidant activity of the oleoresin extracts were later evaluated using DPPH (1, 1-diphenyl-1-picrylhydrazyl) radical scavenging assay. The optimum extraction condition in black pepper refluxation was attained at 90 min irradiation time (x<sub>1</sub>), 350 W power level (x<sub>2</sub>), 0.105 mm feed particle size (x<sub>3</sub>) and 10 mL/g molar ratio (x<sub>4</sub>) with an extremum ranking in decreasing order of x3&gt; x<sub>4</sub>&gt; x<sub>2</sub>&gt; x<sub>1</sub>.However,from the white pepper refluxation an optimum condition was achieved at 120 min irradiation time (x<sub>1</sub>), 350 W power level (x<sub>2</sub>), 0.300 mm feed particle size (x<sub>3</sub>) and 6 mL/g molar ratio (x<sub>4</sub>) with a corresponding decreasing extremum order of x<sub>1</sub>&gt; x<sub>4</sub>&gt; x<sub>2</sub>&gt; x<sub>3</sub>.From the results obtained from concentration-dependent radicals scavenging activity it was concluded that white oleoresins extract were much higher in inhibitory activity than that of black oleoresin extract.</p><p>Chemical Engineering Research Bulletin 19(2017) 139-144</p>

scholarly journals Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 456 ◽  
Author(s):  
Ding-Tao Wu ◽  
Wen Liu ◽  
Mei-Lin Xian ◽  
Gang Du ◽  
Xin Liu ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Antioxidant Activities ◽  
Extraction Methods ◽  
Water Extraction ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Hovenia Dulcis

Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from Hovenia dulcis. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.

scholarly journals Formulation, Characterization and Antioxidant Myricetin Nanophytosome for Topical Delivery

2020 ◽  
Vol 8 (3) ◽  
pp. 9-13
Author(s):  
Purnamasari Nur Aini ◽  
Dzakwan Muhammad ◽  
Pramukantoro Ganet Eko ◽  
Mauludin Rachmat ◽  
El Fahmi
Keyword(s):  
Particle Size ◽  
Encapsulation Efficiency ◽  
Antioxidant Activities ◽  
Biological Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Spherical Shape ◽  
Molar Ratio ◽  
Limiting Factor ◽  
Particle Size Analyzer

Background: Phytosomes are recently introduced drug delivery system and novel botanical formulation to produce lipophilic molecular complex to improve absorption and bioavailability of phytoconstituent. Myricetin is a well-known flavonoid with different biological effects and contributed in food preserving by free radical scavenging activity. However, bioavailability of myricetin is an important limiting factor for its antioxidant activities. Purpose: To overcome this limitation, in the present study we aimed to produce myricetin-loaded nano phytosomes to improve its physicochemical stability and bioavailability. Methods: myricetin-loaded nano phytosome was prepared by using phosphatidylcholine (PC) and cholesterol (CH). Myricetin nanophytosomes system was characterized by particle size analyzer, particle size distribution (PDI), encapsulation efficiency and potential antioxidant activity. Results: Results showed that formulation with the myricetin: PC: CH molar ratio of 1: 2: 0.8 had lower particle size (291.11 nm) and higher encapsulation efficiency percent (93%). Morphology analysis showed that myricetin nanophytosome spherical shape. The potential antioxidant data showed that incorporation myricetin in the phospholipid myricetin remained unchanged even after encapsulation of myricetin in binarynanophytosome formulation. Conclusion: Nano phytosomal formulation of myricetin showed promising potential in fortification of nutraceutical with water insoluble antioxidants.  

scholarly journals Chemical Composition and Biological Activities of Essential Oils from the Leaves, Stems, and Roots of Kadsura coccinea

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6259
Author(s):  
Tianming Zhao ◽  
Chao Ma ◽  
Guofei Zhu
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Cholinesterase Inhibition ◽  
Pharmaceutical Industries ◽  
Kadsura Coccinea ◽  
Radical Scavenging Assay

The chemical composition and biological activities of the essential oils from the leaves, stems, and roots of Kadsura coccinea (K. coccinea) were investigated. The essential oils were extracted by hydro distillation and analyzed by gas chromatography mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). Antioxidant activities of the essential oils were examined with DPPH radical scavenging assay, ABTS cation radical scavenging assay, and ferric reducing antioxidant power assay. Antimicrobial activities were evaluated by determining minimum inhibitory concentrations (MIC) and minimum microbiocidal concentrations (MMC). Acetylcholinesterase and butyrylcholinesterase inhibitory activity of the essential oils were also tested. A total of 46, 44, and 47 components were identified in the leaf, stem, and root oils, representing 95.66%, 97.35%, and 92.72% of total composition, respectively. The major compounds of three essential oils were α-pinene (16.60–42.02%), β-pinene (10.03–18.82%), camphene (1.56–10.95%), borneol (0.50–7.71%), δ-cadinene (1.52–7.06%), and β-elemene (1.86–4.45%). The essential oils were found to have weak antioxidant activities and cholinesterase inhibition activities. The essential oils showed more inhibitory effects against Staphylococcus aureus (S. aureus) than those of other strains. The highest antimicrobial activity was observed in the root oil against S. aureus, with MIC of 0.78 mg/mL. Therefore, K. coccinea essential oils might be considered as a natural antibacterial agent against S. aureus with potential application in food and pharmaceutical industries.

scholarly journals Geranyl Flavonoids from Robinia pseudoacacia

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800
Author(s):  
Lai-Bin Zhang ◽  
Jie-Li Lv ◽  
Hui Zhang
Keyword(s):  
Antioxidant Activities ◽  
Robinia Pseudoacacia ◽  
Radical Scavenging ◽  
Spectroscopic Methods ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

A new geranyl flavonol, robipseudin A (1), and a known geranyl flavone, kuwanon S (2), were isolated from the leaves of Robinia pseudoacacia. The structure of the new compound was determined by spectroscopic methods. Compounds 1 and 2 showed moderate antioxidant activities in the DPPH radical scavenging assay.

scholarly journals Investigation of the nutritional value and antioxidant activities of common Bangladeshi edible mushrooms

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Nazifa Sifat ◽  
Farhana Lovely ◽  
S. M. Neamul Kabir Zihad ◽  
Md. Golam Hossain ◽  
Jamil A. Shilpi ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Composition Analysis ◽  
Scavenging Activity ◽  
Crude Fibre ◽  
Glucose Content ◽  
Total Antioxidant

Abstract Background Mushrooms have been recognized both as medicine and nutritional food in many countries for thousands of years, owing to the presence of significant amounts of carbohydrates, proteins, lipids, fibers, and antioxidants. This study aims at evaluating the nutritional contents and antioxidant potentials of seven types of mushrooms cultivated in Bangladesh. Methods Proximate composition analysis of the collected mushrooms was performed to determine moisture content, pH, lipid, crude fibre, total ash, protein, carbohydrate content, and calorific value. Antioxidant potential of collected mushrooms were evaluated by mean of eight different methods including total flavonoid content, phenolic content, tannin content, total antioxidant activity, hydroxyl radical scavenging activity, DPPH assay and reducing power capacity. Results The results demonstrated that investigated mushrooms were found rich in proteins (20–45 g/100 g), carbohydrate (11–61 g/100 g in dry sample) and fibre (5–40 g/100 g). The ash content was found 6–10 g/100 g and glucose content 54–160 mg/100 g. However, all the mushrooms showed a lower content of lipid (1–4%). Results also revealed that the total antioxidant capacity of the extracts were found in the concentration range of 0.08–0.21 mg/mL, whereas the hydroxyl radical and DPPH radical scavenging activity were 0.88–1.40 and 0.05–0.63 mg/mL. Conclusion The findings of the current investigation proved that the studied Bangladeshi mushrooms are good source of nutritional and antioxidant components. Therefore, this study can help spreading awareness among Bangladeshi people regarding consumption of mushrooms as functional foods.

scholarly journals An Investigation on Biological Efficacies of Fruits and Leaves of Ficus auriculata (Timilo) from Kanchanpur District, Nepal

2021 ◽  
Vol 2 (01) ◽  
pp. 51-58
Author(s):  
Bishan Datt Bhatt ◽  
Dharma Raj Joshi
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Dpph Radical ◽  
Leaf Extracts ◽  
E Coli ◽  
Methanol Extracts ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Ficus auriculata is a native Asian plant found in the temperate, tropical and subtropical regions and has been commonly used in traditional medicine and as fodder in animal husbandry. The comparative antibacterial and antioxidant efficacies of leaves and fruits have been studied using their hexane, chloroform, ethyl acetate and methanol extracts. Phytochemical screening exhibited the presence of important secondary metabolites like alkaloids, carbohydrates, glycosides, flavonoids and tannins. Antibacterial activities of fruit and leaf extracts in different concentrations were studied against E. coli, S. aureus and S. typhi by agar well diffusion method. The highest inhibition was found to be in 1% methanol extracts of leaves and fruits with a zone of inhibition (ZOI) ± 16 mm against S. aureus followed by E. coli and S. typhi with ZOI ±14 mm. The crude and 50% extracts of various solvents of both fruits and leaves were found to be ineffective against bacteria. These results reveal that there is a significant antibacterial activity in methanol extract of both fruit and leaves, against gram-positive and gram-negative bacteria. The antioxidant activities of methanol extracts of fruits and leaves were studied by DPPH radical scavenging assay. The IC50 values of methanol extract of leaves and fruit for DPPH radical scavenging assay were found to be 114.84 μg/mL and 78.28 μg/mL, respectively. These results reveal that methanol fruit extract exhibits better antioxidant activity as compared to the leaves. The result of this investigation has revealed the applicability of this plant as a potential source of several bioactive compounds for the discovery of new and efficacious drugs in days to come.

Changes in physicochemical properties and antioxidant activity of fermented kepayang (Pangium edule Reinw.) seeds

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 47-58
Author(s):  
N.H. Mustaffer ◽  
N.S. Ramli ◽  
K. Tongkhao ◽  
K. Vangnai ◽  
N.K. Rosni ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ph Value ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Total Phenolic ◽  
Crude Fibre

One method for processing Pangium edule Reinw. seeds for consumption is fermentation. The aim of this study was to determine the physicochemical characteristics of P. edule Reinw. seeds during fermentation and antioxidant activity of the extracts. P. edule Reinw. seeds were boiled for 2 hrs and subjected to spontaneous fermentation for 40 days. The physicochemical properties were evaluated according to AOAC methods. Raw and fermented P. edule Reinw. seeds (day 40) were dried and extracted using ethanol and distilled water. The antioxidant activity was measured using 2,2-diphenyl-1- dipicrylhydrazyl (DPPH) radical scavenging assay while total phenolic content was measured by following the Folin-Ciocalteu method. The results showed that there were no significant differences in water activity while the pH value was decreased along fermentation days. The TSS was significantly increased from day 0 (11.00) to day 30 (19.50), however, it drastically decreased to 11.50 after 40 days of fermentation. The value of lightness (L*) parameter was decreased from 66.44 to 25.28 on day 40 but no significant differences for a* and b* parameter. For proximate analysis, the percentage of ash, crude protein, crude fat, and crude fibre were significantly increased while moisture content and carbohydrate were significantly decreased throughout the fermentation days. Besides, the highest DPPH activity was observed in the ferment-water extract at concentration 10 mg/mL which about 95.61% while the highest total phenolic content was obtained from the ferment-ethanol extract (173.79 mg GAE/100 mg). In conclusion, there were some changes in the physicochemical properties of P. edule Reinw. seeds during fermentation and potentially has antioxidant activities. The results of this study might be used as basic information to develop the fermented seeds as a functional food.

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