scholarly journals Profiling the Functional Diversity of Termite Mound Soil Bacteria as Revealed by Shotgun Sequencing

Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 637 ◽  
Author(s):  
Ben Jesuorsemwen Enagbonma ◽  
Bukola Rhoda Aremu ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Functional Diversity ◽  
Chemical Properties ◽  
Shotgun Sequencing ◽  
Functional Categories ◽  
Termite Mound ◽  
Β Diversity ◽  
Α Diversity ◽  
Significant Difference ◽  
Commercial Applications ◽  
Physical And Chemical

Profiling the metabolic processes performed by bacteria is vital both for understanding and for manipulating ecosystems for industrial or research purposes. In this study we aim to assess the bacterial functional diversity in termite mound soils with the assumption that significant differences will be observed in the functional diversity of bacteria between the termite mound soils and their surrounding soils and that each environment has a distinguishing metabolic profile. Here, metagenomic DNA extracted from termite mound soils and their corresponding surrounding soils, which are 10 m apart, were sequenced using a shotgun sequencing approach. Our results revealed that the relative abundances of 16 functional categories differed significantly between both habitats. The α diversity analysis indicated no significant difference in bacterial functional categories within the habitats while the β diversity showed that the bacterial functional categories varied significantly between the termite mound soils and the surrounding soil samples. The variations in soil physical and chemical properties existing between the two environments were held accountable for the differences in bacterial functional structure. With the high relative abundance of functional categories with unknown function reported in this study, this could signify the likelihood of getting novel genes from termite mound soils, which are needed for research and commercial applications.

scholarly journals Effect of Mosses on physical and chemical properties of soil in temperate forests of Garhwal Himalayas

2021 ◽  
pp. 1-10
Author(s):  
Anshu Siwach ◽  
Siddhartha Kaushal ◽  
Ratul Baishya
Keyword(s):  
Physical Properties ◽  
Chemical Properties ◽  
Nutrient Status ◽  
High Rate ◽  
Physical And Chemical Properties ◽  
Garhwal Himalayas ◽  
Forest Sites ◽  
Significant Difference ◽  
Physical And Chemical ◽  
And Chemical Properties

Abstract Mosses are one of the most important and dominant plant communities, especially in the temperate biome, and play a significant role in ecosystem function and dynamics. They influence the water, energy and element cycle due to their unique ecology and physiology. The present study was undertaken in three different temperate forest sites in the Garhwal Himalayas, viz., Triyuginarayan (Kedarnath Wildlife Sanctuary (KWLS)), Chakrata, and Kanasar forest range. The study was focused on understanding the influence of mosses on soil physical properties and nutrient availability. Different physico-chemical properties were analysed under two different substrata, that is, with and without moss cover in two different seasons, viz., monsoon and winter. We observed mosses to influence and alter the physical properties and nutrient status of soil in both seasons. All soil physical and chemical properties, except magnesium, showed significant difference within the substrates, among all the sites and across the two seasons. Besides the soil characteristics underneath the moss vegetation, the study also highlights the diversity of mosses found in the area. Mosses appear to create high nutrient microsites via a high rate of organic matter accumulation and retain nutrients for longer periods thus, maintaining ecosystem stability.

scholarly journals A study of Soil Invertebrates community in a Date - Palm plantation in Baghdad, Iraq

2016 ◽  
Vol 13 (1) ◽  
pp. 1-6
Author(s):  
Baghdad Science Journal
Keyword(s):  
Organic Matter ◽  
Direct Method ◽  
Chemical Properties ◽  
Essential Elements ◽  
Soil Samples ◽  
Land Snails ◽  
Soil Invertebrates ◽  
Individual Number ◽  
Significant Difference ◽  
Physical And Chemical

Soil invertebrates community an important role as part of essential food chain and responsible for the decomposition in the soil, helps soil aeration , nutrients recycling and increase agricultural production by providing the essential elements necessary for photosynthesis and energy flow in ecosystems.The aim of the present study was to investigate the soil invertebrates community in one of the date palms plantation in Aljaderia district South of Baghdad, , and their relationships with some physical and chemical properties of the soil , as Five randomly distributed replicates of soil samples were collected monthly. Invertebrates samples were sorted from the soil with two methods, direct method to isolate large invertebrates and indirectly to isolate small invertebrates using wet funnel method. The study also included the determination of physical and chemical factors of the soil (Temperature, Salinity, pH, Organic matter, Humidity, In addition to the soil texture).Monthly fluctuations in physical and chemical characteristics of the soil and the total invertebrates community study site were determined. Significant correlations the of the invertebrates community and each of temperature, organic matter, and humidity were observed. The study revealed that the temperature of the soil ranged between 5 to 25 C0 , The salinity concentration ranged between 1.1-1.9 ‰, The pH values ranged between 7.3 to 7.8 and the percentage of soil moisture ranged between 15 - 25% , Soil samples were composed of 44.6 % Clay, 19.7% Silt and 35.5% Sand.A total of 4625 individuals of soil invertebrates belonging to 16 taxa were sorted , within which the adult and larval insects were the most abundant, and from them 1283 individuals were sorted , represented 28% of the total numbers, followed by Isopoda , which 1030 individuals of them were sorted, In addition to Nematode, Oligochaetes Annelids family Enchytraeidae, and Earthworms family Lumbricida, Species of Chilopoda, Diplopoda, mites, land snails and slugs. The highest total individual number were recorded recorded durim moderate temperature months, February, March and April amounted to 838, 801 and 813 individuals, respectively.A significant correlation was mated between total number of soil invertebrates and each of temperature, organic matter and humidity. The significant difference in means was calculated according to LSD test.

scholarly journals Wheat straw increases the defense response and resistance of watermelon monoculture to Fusarium wilt

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Lili Tang ◽  
Shaorui Nie ◽  
Wenhui Li ◽  
Chao Fan ◽  
Siqi Wang ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Defense Response ◽  
Chemical Properties ◽  
Lignin Biosynthesis ◽  
Induced Response ◽  
Significant Difference ◽  
Phenylpropanoid Biosynthesis ◽  
Straw Return ◽  
Race 1 ◽  
Physical And Chemical

Abstract Background Wheat straw is a rich resource worldwide. Straw return is an effective strategy to alleviate soil-borne diseases on monoculture watermelon. Previous studies focus on soil structure, physical and chemical properties; however, little is known about the molecular responses on host plant. Results No significant difference on the population of Fusarium oxysporum f.sp. niveum race 1(Fon1) in rhizosphere soil was found between control (no addition of wheat straw) and the treated groups (addition of 1% (T1) or 2% (T2) wheat straw). RNA-Seq analysis showed that 3419 differentially expressed genes were clustered into 8 profiles. KEGG analysis revealed that phenylpropanoid biosynthesis and plant hormone signal transduction were involved in wheat straw induced response in monoculture watermelon. Genes in lignin biosynthesis were found to be upregulated, and the lignin and auxin contents were higher in T1 and T2 compared to the control. Lignin was also enriched and the Fon1 population decreased in watermelon roots treated with wheat straw. The enzyme activities of phenylalanine ammonia-lyase and peroxidase were increased. Conclusions Our data suggest that the addition of wheat straw enhances the defense response to Fon1 infection in watermelon through increasing lignin and auxin biosynthesis.

scholarly journals The Effect of Gelatin Addition from Various Fish Skin to the Physical and Chemical Properties of Chikuwa

2019 ◽  
Vol 3 (1) ◽  
pp. 78
Author(s):  
Muhammad Irvan
Keyword(s):  
Protein Content ◽  
Chemical Properties ◽  
Gel Strength ◽  
Chemical Characteristics ◽  
Fish Scale ◽  
Physical And Chemical Properties ◽  
Physical And Chemical Characteristics ◽  
Significant Difference ◽  
Physical And Chemical ◽  
And Chemical Properties

Chikuwa is one of Japanese traditional fishery food product that commonly made from potato starch, fish surimi, and some spices. To enhance Chikuwa physical and chemical properties especially in texture attribute and protein content, Gelatin can be added. Gelatin is a partial hydrolysis protein that usually added in food making process to improve the gumminess quality and the protein content. Gelatin can be derived from bone collagen, skin and fish scale. The purpose of this study is to analyze the effect of gelatin from various skin fish to the physical and chemical characteristics of Chikuwa. The research method used is experimental laboratories using a completely randomized design (CRD) with 3 replications. The data analysed with ANOVA and continued with BNJ analysis if there is a significant difference between the treatments. This research has divided into two stages. The first step aim is to make the gelatin from the skin of seabass, payus fish and tilapia with 3% concentrations. The second step is Chikuwa making added with gelatin. The parameters that observed are water content, protein content, white degree, gel strength, sensory attributes, folding, bite. The results showed that Chikuwa with the addition of gelatin from seabass, payus and tilapia skin are significantly different (p <0.05) due to the physical and chemical characteristics of Chikuwa. The best Chikuwa quality is Chikuwa with the addition of seabass gelatin, where the gelatin yield is 18.03 ± 0.68; the gelatin gel strength is 251.11 ± 1.08 bloom; the viscosity is 5.80 ± 0.15 cP; the gel Chikuwa sample strength is 954.54 ± 0.56 gcm and protein content is 22.01 ± 0.98%

scholarly journals Disentangling the Association of Corn Root Mycobiome With Plant Productivity and the Importance of Soil Physicochemical Balance in Shaping Their Relationship

2021 ◽  
Vol 5 ◽  
Author(s):  
Saveetha Kandasamy ◽  
Nimalka Weerasuriya ◽  
Geetha Subramanian ◽  
R. Greg Thorn ◽  
Greg Patterson ◽  
...  
Keyword(s):  
Infrared Imaging ◽  
Crop Yields ◽  
Chemical Properties ◽  
Plant Productivity ◽  
Crop Productivity ◽  
High Yield ◽  
Major Influence ◽  
Fungal Abundance ◽  
Α Diversity ◽  
Physical And Chemical

Soil bacteria and fungi are integral parts of healthy ecosystem functioning in production agriculture. The effects of fungal abundance and diversity on crop productivity is poorly understood. We sampled 10 corn farms at the V10 growth stage across southwestern Ontario, Canada, using aerial infrared imaging to identify zones of low and high productive corn plants. Roots and soils were sampled from low and high yield zones and soil physical and chemical properties were measured in conjunction with assessment of the root mycobiome communities using Illumina MiSeq sequencing of 4 rRNA amplicons. Higher crop yields were associated with sites having greater fungal phylogenetic diversity and Fisher's α diversity. Indicator species associated with high and low yield sites within a farm could be identified but there were no shared fungal indicators of productivity differences across farms. Communities largely varied across locations despite crop genetics, demonstrating a major influence of soil texture and chemistry in shaping the mycobiome in a site-specific manner. Across all 4 primers, roots from high-yielding sites shared 35 major OTUs including Penicillium spp., Trichoderma, Chalara fungorum, and Gibellulopsis. Low-yielding sites shared 31 OTUs including Fusarium spp., Pythium, Setophoma terrestris, and Neonectria. Soil physical and chemical parameters that contributed to broad scale differences in yield and mycobiome diversity included: %clay, %sand, %phosphorus saturation, cation exchange capacity, aluminum, pH, iron, potassium, %moisture, organic matter, and chlorine. The results show the importance of physicochemical balance in shaping the relationship between root mycobiome and plant productivity.

scholarly journals Effect of agrotechnical factors on the activity of urease enzyme in a long term fertlization experiment

2016 ◽  
pp. 43-48
Author(s):  
Judit Horváth ◽  
Bence Mátyás ◽  
János Kátai
Keyword(s):  
Organic Matter ◽  
Extracellular Enzymes ◽  
Chemical Properties ◽  
Accurate Information ◽  
The Sustainable Development ◽  
Urease Enzyme ◽  
Significant Difference ◽  
Physical And Chemical ◽  
Second Period

The soil is a natural resource, the fertility preservation is an important part of the sustainable development. We have to monitor the transformation dinamics of the organic nitrogen-containing substances, to get accurate information about the changes of the nitrogen cycle in the soil. Physical and chemical properties of the soil and the microorganism effect on the organic matter in the soil – in addition to the composition of organic matter. Wide variety of extracellular enzymes are present in this decomposition. These enzymes help in the transformation of the macromolecules to transforming low molecular weight compounds so they will be available during the assimilation. The urease enzyme, catalyzes the hydrolysis of urea to CO2 and NH3. The urease is widely spread in the nature, it is present in the microorganisms, plants and animals. We found that the soil moisture content, the rotation and the fertilization affect to the amount of urease in spring. Furthermore, we get significant difference between the irrigated and non irrigated samples in the second period of the year. Based on our results we can state that the activity of urease was higher in spring 2014. The objective of our study was to present how the different agronomic factors affect on the activity of urease in a long term fertilizationexperiment.

scholarly journals Influences of Land Use/ Cover Changes on Soil Properties in Rib Watershed, Ethiopia

2020 ◽  
Author(s):  
Fentanesh Haile Buruso ◽  
Zenebe Admasu Teferi
Keyword(s):  
Land Use ◽  
Soil Properties ◽  
Chemical Properties ◽  
Temporal Variations ◽  
Natural Forest ◽  
Physical And Chemical Properties ◽  
Natural Forests ◽  
Significant Difference ◽  
Physical And Chemical ◽  
And Chemical Properties

Abstract BackgroundThe decrease in the area under natural vegetation and its conversion into other types of use has resulted in resource degradation including soil quality loss. Soil properties response to changes in land use/ cover has shown spatial and temporal variations. Hence this study was carried out to examine the influence of land use/ cover changes on physical and chemical properties of the soils in Rib watershed. Soil samples were taken over three selected land use/ covers (natural forest, grazing and cultivated lands) in two agro- ecological zones (Dega and High Dega). Multivariate analysis of variance (MNOVA) and Pearson’s correlation was computed. ResultsThe study revealed that land use/ cover and altitude have influenced physical and chemical properties of the soil in the study watershed. Significant difference in distribution of soil texture, BD, OC, TN and pH among land use/ covers have been observed. Natural forest had higher OC, OC stock and TN than grazing and cultivated lands. The mean OC stock ranged from 188.32 t/ha in natural forest to 72.75t/ha in cultivated lands. Soil pH was slightly higher for natural forests and lower in the soils of grazing and cultivated lands. Significant difference (P<0.05) among the two agro ecologies were also observed in OC, Ca2+, clay, and silt.. ConclusionTherefore, land use/ cover changes have affected the concentration of TN, OC, increase soil acidity and compaction that can affect productive of soils and production of crops.

scholarly journals The Dynamics of Respiratory Microbiota during Mechanical Ventilation in Patients with Pneumonia

2020 ◽  
Vol 9 (3) ◽  
pp. 638 ◽  
Author(s):  
Seongji Woo ◽  
So-Yeong Park ◽  
Youngmi Kim ◽  
Jin Pyeong Jeon ◽  
Jae Jun Lee ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Intensive Care ◽  
Clinical Outcomes ◽  
Extubation Failure ◽  
Shannon Index ◽  
Β Diversity ◽  
Α Diversity ◽  
Significant Difference ◽  
Respiratory Microbiome ◽  
Successful Extubation

Bacterial pneumonia is a major cause of mechanical ventilation in intensive care units. We hypothesized that the presence of particular microbiota in endotracheal tube aspirates during the course of intubation was associated with clinical outcomes such as extubation failure or 28-day mortality. Sixty mechanically ventilated ICU (intensive care unit) patients (41 patients with pneumonia and 19 patients without pneumonia) were included, and tracheal aspirates were obtained on days 1, 3, and 7. Gene sequencing of 16S rRNA was used to measure the composition of the respiratory microbiome. A total of 216 endotracheal aspirates were obtained from 60 patients. A total of 22 patients were successfully extubatedwithin3 weeks, and 12 patients died within 28days. Microbiota profiles differed significantly between the pneumonia group and the non-pneumonia group (Adonis, p < 0.01). While α diversity (Shannon index) significantly decreased between day 1 and day 7 in the successful extubation group, it did not decrease in the failed extubation group among intubated patients with pneumonia. There was a significant difference in the change of βdiversity between the successful extubation group and the failed extubation group for Bray-Curtis distances (p < 0.001). At the genus level, Rothia, Streptococcus, and Prevotella correlated with the change of β diversity. A low relative abundance of Streptococci at the time of intubation was strongly associated with 28-day mortality. The dynamics of respiratory microbiome were associated with clinical outcomes such as extubation failure and mortality. Further large prospective studies are needed to test the predictive value of endotracheal aspirates in intubated patients.

scholarly journals Termite Societies Promote the Taxonomic and Functional Diversity of Archaeal Communities in Mound Soils

Biology ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 136
Author(s):  
Monde Wakung’oli ◽  
Adenike Eunice Amoo ◽  
Ben Jesuorsemwen Enagbonma ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Functional Diversity ◽  
Alpha Diversity ◽  
Taxonomic Composition ◽  
Archaeal Diversity ◽  
Functional Categories ◽  
Termite Mound ◽  
Termite Mounds ◽  
Sequencing Method ◽  
Bacteria And Fungi ◽  
Archaeal Communities

Recent studies involving microbial communities in termite mounds have been more focused on bacteria and fungi with little attention given to archaea, which play significant roles in nutrient cycling. Thus, we aimed at characterizing the archaeal taxonomic and functional diversity in two termite mound soils using the shotgun sequencing method with the assumption that termite activities could promote archaeal diversity. Our findings showed that termite mound soils have archaeal groups that are taxonomically different from their surrounding soils, with Euryarchaeota, Korarchaeota, and Nanoarchaeota being predominant while Thaumarchaeota and Crenarchaeota were predominant in the surrounding soils. Additionally, the observed nutrient pathways: phosphorus, nitrogen, and sulfur were all significantly more predominant in termite mound soils than in their comparative surrounding soils. Alpha diversity showed that archaea were not significantly different within termite mound soils and the surrounding soils. The beta diversity revealed significant differences in the archaeal taxonomic composition and their functional categories between the termite mounds and surrounding soils. Our canonical correspondence analysis revealed that the distribution of archaeal communities was likely dependent on the soil properties. Our results suggested that termite activities may promote the diversity of archaea; with some of our sequences grouped as unclassified archaea, there is a need for further research to unveil their identity.

scholarly journals PENGARUH KONSENTRASI LARUTAN KALSIUM HIDROKSIDA (Ca (OH)2) TERHADAP KARAKTERISTIK FISIK DAN KIMIA GELATIN KULIT SAPI

2019 ◽  
Vol 15 (1) ◽  
pp. 217
Author(s):  
Susan ., Mokoolang ◽  
Meity ., Sompie ◽  
Indiyah ., Wahyuni
Keyword(s):  
Calcium Hydroxide ◽  
Chemical Properties ◽  
Animal Husbandry ◽  
Food Engineering ◽  
Animal Product ◽  
Randomized Design ◽  
Significant Difference ◽  
Lime Solution ◽  
Product Technology ◽  
Physical And Chemical

The aim of the study was to examine the effect of differences in the concentration of lime solution (Ca (OH) 2) on the physical and chemical properties of gelatin in cow skin. This research was conducted in December 2017 until January 2018 at the Laboratory of Animal Product Technology at the Faculty of Animal Husbandry, Sam Ratulangi University of Manado and analysis was carried out at the Food Engineering Laboratory, Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta. This study used a Completely Randomized Design (CRD) 4 x 4 with the treatment of the concentration of calcium hydroxide (Ca (OH) 2) solution, each treatment was repeated four times (Steel and Torrie, 2001). The results showed that the treatment of the concentration of calcium hydroxide (Ca (OH) 2) gave no significant difference (P> 0.05) on gel strength, viscosity, yield, pH and water content of gelatin in cow skin.*eprm*

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