scholarly journals Modification of Luffa Sponge for Enrichment of Phosphopeptides

2019 ◽  
Vol 21 (1) ◽  
pp. 101 ◽  
Author(s):  
Lili Dai ◽  
Zhe Sun ◽  
Ping Zhou
Keyword(s):  
Solid Phase ◽  
Cost Effective ◽  
Molar Ratio ◽  
Porous Texture ◽  
Enrichment Technique ◽  
Protein Digests ◽  
Strong Anion Exchange ◽  
Set Up ◽  
Spe Method ◽  
Luffa Sponge

The enrichment technique is crucial to the comprehensive analysis of protein phosphorylation. In this work, a facile, green and efficient synthetic method was set up for quaternization of luffa sponge. The resultant luffa sponge showed strong anion-exchange characteristics and a high adsorption ability for phosphate ions. Along with the unique physical properties, e.g., tenacity and porous texture, quaternized luffa sponge was demonstrated to be a well-suited solid-phase extraction (SPE) material. The quaternized luffa sponge-based SPE method was simple, cost-effective and convenient in operation, and was successfully applied to the capture of phosphopeptides from protein digests. The enrichment approach exhibited exceptionally high selectivity, sensitivity and strong anti-interference ability. Four phosphopeptides were still detected by using the digest mixture of β-casein and bovine serum albumin with a molar ratio of 1:100. 21 phosphopeptides were identified from the tryptic digest of non-fat milk.

scholarly journals Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods

2021 ◽  
Vol 21 (4) ◽  
pp. 806
Author(s):  
Orchidea Rachmaniah ◽  
Muhammad Rifqy Muhsin ◽  
Angga Widya Putra ◽  
Muhammad Rachimoellah
Keyword(s):  
Acetic Acid ◽  
Solid Phase ◽  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Extraction Process ◽  
Acid Water ◽  
Molar Ratio ◽  
Separation And Purification ◽  
Natural Deep Eutectic Solvents ◽  
Spe Method

Curcuminoids can be successfully extracted from Curcuma zedoaria using natural deep eutectic solvents (NADES) as extraction solvents. However, a mixture of extracted curcuminoids, NADES, and impurities from C. zedoaria was obtained as a slurry at the end of the extraction process. Therefore, further separation and purification were required to obtain the extracted compound in high purity. Herein, two purification methods based on classical column chromatography (CCC) and solid-phase extraction (SPE), were evaluated for the purification of curcuminoids from NADES matrices after extraction. Choline chloride–malic acid–water (CCMA–H2O) and choline chloride–citric acid–water (CCCA–H2O) in the molar ratio of 1:1:18 were selected as NADES matrices due to their high solubility and stabilization capability for curcuminoids. Ethanol-conditioned silica gel (60–200 µm) was applied as the bed resin for CCC, and a C18 cartridge was used for SPE. Acetonitrile/0.1% acetic acid, water/0.1% acetic acid, and iso-propanol/0.1% acetic acid were used as mobile phases for CCC. For SPE, methanol/0.05% acetic acid and water/0.05% acetic acid were applied in the conditioning step, water/0.05% acetic acid in the washing step, and methanol/acetonitrile (1:1) in the eluting step. The SPE method produced higher recovery of curcuminoids from the CCCA–H2O and CCMA–H2O matrices (75.27% and 73.40%, respectively) compared to CCC (51.9% and 61.0%, respectively). After removing the NADES constituents from the crude extract of curcuminoids, recrystallization was attempted.

Corncob Waste Based Adsorbent for Solid Phase Extraction of Tartrazine in Carbonated Drinks and Analytical Method using Ultra Performance Liquid Chromatography-Mass Spectrometry

2020 ◽  
Vol 16 (7) ◽  
pp. 924-932
Author(s):  
Yasmeen Mutlaq Ghazi Al Shamari ◽  
Saikh Mohammad Wabaidur ◽  
Abdulrahman Abdullah Alwarthan ◽  
Moonis Ali Khan ◽  
Masoom Raza Siddiqui
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Cost Effective ◽  
Ultra Performance Liquid Chromatography ◽  
Phase Extraction ◽  
Food Dye ◽  
Hyphenated Technique

Background : A new method has been developed for the determination of food dye tartrazine in soft drinks. Tartrazine is determined by hyphenated technique Ultra Performance Liquid Chromatography coupled with Mass spectrometry. The solid-phase extraction was used for the extraction of tartrazine. Methods: For the LC-MS analysis of tartrazine acetonitrile, water (80:20) was used as a mobile phase whereas, the C-18 column was selected as the stationary phase. The chromatographic run was allowed for 1 min. The adsorbent of the solid-phase extraction was synthesized from the waste corn cob. Results: Method found to be linear in the range of 0.1 mg L-1 - 10 mg L-1, limits of detection and quantitation were found to be 0.0165 mgL-1 and 0.055 mgL-1, respectively. Tartrazine, in the real sample, was found to be 20.39 mgL-1 and 83.26 mgL-1. Conclusion: The developed UPLC-MS method is rapid, simple, precise and can be used for the quantitative analysis of tartrazine. The solid-phase extraction also involves a cost-effective procedure for extraction as it does not involve the commercial cartridge.

scholarly journals Environmental Engineering Techniques to Restore Degraded Posidonia oceanica Meadows

Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 661
Author(s):  
Luigi Piazzi ◽  
Stefano Acunto ◽  
Francesca Frau ◽  
Fabrizio Atzori ◽  
Maria Francesca Cinti ◽  
...  
Keyword(s):  
Large Scale ◽  
Natural Habitat ◽  
Cost Benefit ◽  
Cost Effective ◽  
Posidonia Oceanica ◽  
Environmental Engineering ◽  
Bottom Surface ◽  
Sessile Invertebrates ◽  
Set Up ◽  
Short Time

Seagrass planting techniques have shown to be an effective tool for restoring degraded meadows and ecosystem function. In the Mediterranean Sea, most restoration efforts have been addressed to the endemic seagrass Posidonia oceanica, but cost-benefit analyses have shown unpromising results. This study aimed at evaluating the effectiveness of environmental engineering techniques generally employed in terrestrial systems to restore the P. oceanica meadows: two different restoration efforts were considered, either exploring non-degradable mats or, for the first time, degradable mats. Both of them provided encouraging results, as the loss of transplanting plots was null or very low and the survival of cuttings stabilized to about 50%. Data collected are to be considered positive as the survived cuttings are enough to allow the future spread of the patches. The utilized techniques provided a cost-effective restoration tool likely affordable for large-scale projects, as the methods allowed to set up a wide bottom surface to restore in a relatively short time without any particular expensive device. Moreover, the mats, comparing with other anchoring methods, enhanced the colonization of other organisms such as macroalgae and sessile invertebrates, contributing to generate a natural habitat.

scholarly journals K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Peio Ziarsolo ◽  
Tomas Hasing ◽  
Rebeca Hilario ◽  
Victor Garcia-Carpintero ◽  
Jose Blanca ◽  
...  
Keyword(s):  
Cost Effective ◽  
Genetic Distances ◽  
Set Design ◽  
Special Equipment ◽  
Genetic Studies ◽  
Set Up ◽  
Genomic Regions ◽  
Biology Laboratory ◽  
Genotyping Technology ◽  
Genetic Results

Abstract Background K-seq, a new genotyping methodology based on the amplification of genomic regions using two steps of Klenow amplification with short oligonucleotides, followed by standard PCR and Illumina sequencing, is presented. The protocol was accompanied by software developed to aid with primer set design. Results As the first examples, K-seq in species as diverse as tomato, dog and wheat was developed. K-seq provided genetic distances similar to those based on WGS in dogs. Experiments comparing K-seq and GBS in tomato showed similar genetic results, although K-seq had the advantage of finding more SNPs for the same number of Illumina reads. The technology reproducibility was tested with two independent runs of the tomato samples, and the correlation coefficient of the SNP coverages between samples was 0.8 and the genotype match was above 94%. K-seq also proved to be useful in polyploid species. The wheat samples generated specific markers for all subgenomes, and the SNPs generated from the diploid ancestors were located in the expected subgenome with accuracies greater than 80%. Conclusion K-seq is an open, patent-unencumbered, easy-to-set-up, cost-effective and reliable technology ready to be used by any molecular biology laboratory without special equipment in many genetic studies.

scholarly journals Sustainable Ventilation Strategies for a Medium-Sized Space with Regional Effect

2021 ◽  
Vol 13 (9) ◽  
pp. 4651
Author(s):  
Ming-Lun Alan Fong
Keyword(s):  
Life Cycle ◽  
Life Cycle Cost ◽  
Co2 Emission ◽  
High Efficiency ◽  
Regional Climate ◽  
Cost Effective ◽  
Regional Effect ◽  
Climate Conditions ◽  
Ventilation Strategies ◽  
Set Up

The analysis of ventilation strategies is fundamentally affected by regional climate conditions and local cost databases, in terms of energy consumption, CO2 emission and cost-effective analysis. A systematic approach is covered in this paper to estimate a local economic and environmental impact on a medium-sized space located in two regions during supply-and-installation and operation phases. Three ventilation strategies, including mixing ventilation (MV), displacement ventilation (DV) and stratum ventilation (SV) were applied to medium-sized air-conditioned space with this approach. The trend of the results for three ventilation systems in the life cycle assessment (LCA) and life cycle cost (LCC) analysis is SV < DV < MV. The result of CO2 emission and regional LCC shows that SV is the lowest one in both regional studies. In comparison with the Hong Kong Special Administrative Region (HKSAR) during 20 Service years, the case analysis demonstrates that the percentage differences in LCC analysis of MV, DV & SV in Guangdong are less than 20.5%, 19.4% and 18.82% respectively. Their CO2 emission of MV, DV and SV in Guangdong are more than HKSAR in 10.69%, 11.22% and 12.05%, respectively. The present study could provide information about regional effects in the LCA and LCC analysis of three ventilation strategies emissions, and thereby help set up models for decision-making on high efficiency and cost-effective ventilation strategy plans.

scholarly journals Biodiesel Production from Melia azedarach and Ricinus communis Oil by Transesterification Process

Catalysts ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 427 ◽  
Author(s):  
Muhammad Awais ◽  
Sa’ed A Musmar ◽  
Faryal Kabir ◽  
Iram Batool ◽  
Muhammad Asif Rasheed ◽  
...  
Keyword(s):  
Ricinus Communis ◽  
Cost Effective ◽  
Biodiesel Production ◽  
Molar Ratio ◽  
Melia Azedarach ◽  
Renewable Fuel ◽  
Animal Fats ◽  
Edible Crops ◽  
American Society

Biodiesel is a renewable fuel usually produced from vegetable oils and animal fats. This study investigates the extraction of oil and its conversion into biodiesel by base-catalyzed transesterification. Firstly, the effect of various solvents (methanol, n-hexane, chloroform, di-ethyl ether) on extraction of oil from non-edible crops, such as R. communis and M. azedarach, were examined. It was observed that a higher concentration of oil was obtained from R. communis (43.6%) as compared to M. azedarach (35.6%) by using methanol and n-hexane, respectively. The extracted oils were subjected to NaOH (1%) catalyzed transesterification by analyzing the effect of oil/methanol molar ratio (1:4, 1:6, 1:8 and 1:10) and varying temperature (20, 40, 60 and 80 °C) for 2.5 h of reaction time. M. azedarach yielded 88% and R. communis yielded 93% biodiesel in 1:6 and 1:8 molar concentrations at ambient temperature whereas, 60 °C was selected as an optimum temperature, giving 90% (M. azedarach) and 94% (R. communis) biodiesel. The extracted oil and biodiesel were characterized for various parameters and most of the properties fulfilled the American Society for Testing and Materials (ASTM) standard biodiesel. The further characterization of fatty acids was done by Gas Chromatography/Mass Spectrometer (GC/MS) and oleic acid was found to be dominant in M. azedarach (61.5%) and R. communis contained ricinoleic acid (75.53%). Furthermore, the functional groups were analyzed by Fourier Transform Infrared Spectroscopy. The results suggested that both of the oils are easily available and can be used for commercial biodiesel production at a cost-effective scale.

Cost Effective Swath Bathymetry

2001 ◽  
Vol 35 (4) ◽  
pp. 33-45 ◽  
Author(s):  
Peter Hogarth
Keyword(s):  
Processing Time ◽  
Cost Effective ◽  
High Accuracy ◽  
High Coverage ◽  
Swath Bathymetry ◽  
World Survey ◽  
Set Up ◽  
Very High ◽  
Harbor Area ◽  
Wide Swath

Between 23rd and 25th July 2001 GeoSwath, a high specification shallow water wide swath bathymetry system, was used to survey the entire Portsmouth NH Harbor area. This paper deals with the results of this survey, illustrating the potential for significant reductions in the high costs, which have prevented widespread proliferation of Swath Bathymetry systems to date. Data, including a complete DTM gridded to 1 m resolution, will be presented and discussed in detail. These results show that the system is very easy to set up and use, requires greatly reduced boat and processing time, whilst offering high accuracy and very high coverage and resolution when used in a real-world survey of a dynamic harbor environment.

scholarly journals Innovation Pathways in the NHS: An Introductory Review

2021 ◽  
Author(s):  
Anmol Arora ◽  
Andrew Wright ◽  
Mark Cheng ◽  
Zahra Khwaja ◽  
Matthew Seah
Keyword(s):  
Health Care ◽  
National Health ◽  
Health Care Professionals ◽  
New Technologies ◽  
Care Delivery ◽  
Cost Effective ◽  
Care Models ◽  
Set Up ◽  
The Uk ◽  
Introductory Review

AbstractHealthcare as an industry is recognised as one of the most innovative. Despite heavy regulation, there is substantial scope for new technologies and care models to not only boost patient outcomes but to do so at reduced cost to healthcare systems and consumers. Promoting innovation within national health systems such as the National Health Service (NHS) in the United Kingdom (UK) has been set as a key target for health care professionals and policy makers. However, while the UK has a world-class biomedical research industry, several reports in the last twenty years have highlighted the difficulties faced by the NHS in encouraging and adopting innovations, with the journey from idea to implementation of health technology often taking years and being very expensive, with a high failure rate. This has led to the establishment of several innovation pathways within and around the NHS, to encourage the invention, development and implementation of cost-effective technologies that improve health care delivery. These pathways span local, regional and national health infrastructure. They operate at different stages of the innovation pipeline, with their scope and work defined by location, technology area or industry sector, based on the specific problem identified when they were set up. In this introductory review, we outline each of the major innovation pathways operating at local, regional and national levels across the NHS, including their history, governance, operating procedures and areas of expertise. The extent to which innovation pathways address current challenges faced by innovators is discussed, as well as areas for improvement and future study.

Removal of organic dye compounds in water and wastewater samples based on covalent organic frameworks -titanium dioxide before analysis by UV-VIS spectroscopy

2021 ◽  
Vol 4 (01) ◽  
pp. 58-67
Author(s):  
Aida Bahadori Bahadori ◽  
Mehdi Ranjbar Corresponding
Keyword(s):  
Titanium Dioxide ◽  
Carbon Nanostructures ◽  
Solid Phase ◽  
Cost Effective ◽  
Combustion Method ◽  
Organic Dye ◽  
Coefficient Of Determination ◽  
Covalent Organic Frameworks ◽  
Methylene Orange ◽  
Vis Spectroscopy

A simple and rapid microwave-assisted combustion method was developed to synthesize homogenous carbon nanostructures (HCNS). This research presents a new and novel nanocomposite structures for removal of methylene red (2-(4- Dimethylaminophenylazo)benzoic acid), methylene orange (4-[4-(Dimethylamino)phenylazo]benzenesulfonic acid sodium salt) and methylene blue (3,7-bis(Dimethylamino)phenazathionium chloride)with semi degradation-adsorption solid phase extraction (SDA-SPE) procedure before determination by UV-VIS spectroscopy. A covalent organic frameworks (COFs) with high purity were synthesized and characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results indicated that the self-assembled carbon nanostructures (COFs) synthesized with the cost-effective method which was used as a novel adsorbent for adsorption of dyes after semi-degradation of methylene red, orange and blue (1-5 mg L-1) as an organic dye by titanium dioxide (TiO2) nanoparticales in presence of UV radiation. Based on results, the COFs/TiO2 has good agreement with the Langmuir adsorption isotherm model with favorite coefficient of determination (R2= 0.9989). The recovery of dye removal based on semi-degradation/adsorption of COFs/TiO2 and adsorption of COFs were obtained 98.7% and 48.3%, respectively (RSD less than 5%). The method was validated by spiking dye to real samples.

Modification of turbulence caused by cationic surfactant wormlike micellar structures in two-dimensional turbulent flow

2021 ◽  
Vol 933 ◽  
Author(s):  
Kengo Fukushima ◽  
Haruki Kishi ◽  
Hiroshi Suzuki ◽  
Ruri Hidema
Keyword(s):  
Turbulent Flow ◽  
Surfactant Solution ◽  
Turbulent Energy ◽  
Molar Ratio ◽  
Wormlike Micelles ◽  
Two Dimensional ◽  
Turbulence Statistics ◽  
Image Velocimetry ◽  
Set Up ◽  
Induced Structure

An experimental study is performed to investigate the effects of the extensional rheological properties of drag-reducing wormlike micellar solutions on the vortex deformation and turbulence statistics in two-dimensional (2-D) turbulent flow. A self-standing 2-D turbulent flow was used as the experimental set-up, and the flow was observed through interference pattern monitoring and particle image velocimetry. Vortex shedding and turbulence statistics in the flow were affected by the formation of wormlike micelles and were enhanced by increasing the molar ratio of the counter-ion supplier to the surfactant, ξ, or by applying extensional stresses to the solution. In the 2-D turbulent flow, extensional and shear rates were applied to the fluids around a comb of equally spaced cylinders. This induced the formation of a structure made of wormlike micelles just behind the cylinder. The flow-induced structure influenced the velocity fields around the comb and the turbulence statistics. A characteristic increase in turbulent energy was observed, which decreased slowly downstream. The results implied that the characteristic modification of the 2-D turbulent flow of the drag-reducing surfactant solution was affected by the formation and slow relaxation of the flow-induced structure. The relaxation process of the flow-induced structure made of wormlike micelles was very different from that of the polymers.

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