scholarly journals Acceleration of Carbon Fixation in Chilling-Sensitive Banana under Mild and Moderate Chilling Stresses

2020 ◽  
Vol 21 (23) ◽  
pp. 9326
Author(s):  
Jing Liu ◽  
Tomáš Takáč ◽  
Ganjun Yi ◽  
Houbin Chen ◽  
Yingying Wang ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Plant Hormone ◽  
Carbon Fixation ◽  
Molecular Level ◽  
Chilling Stress ◽  
Digital Gene Expression ◽  
Tropical Plant ◽  
Photosynthesis Efficiency ◽  
Immunofluorescence Labeling ◽  
Upregulated Genes

Banana is one of the most important food and fruit crops in the world and its growth is ceasing at 10–17 °C. However, the mechanisms determining the tolerance of banana to mild (>15 °C) and moderate chilling (10–15 °C) are elusive. Furthermore, the biochemical controls over the photosynthesis in tropical plant species at low temperatures above 10 °C is not well understood. The purpose of this research was to reveal the response of chilling-sensitive banana to mild (16 °C) and moderate chilling stress (10 °C) at the molecular (transcripts, proteins) and physiological levels. The results showed different transcriptome responses between mild and moderate chilling stresses, especially in pathways of plant hormone signal transduction, ABC transporters, ubiquinone, and other terpenoid-quinone biosynthesis. Interestingly, functions related to carbon fixation were assigned preferentially to upregulated genes/proteins, while photosynthesis and photosynthesis-antenna proteins were downregulated at 10 °C, as revealed by both digital gene expression and proteomic analysis. These results were confirmed by qPCR and immunofluorescence labeling methods. Conclusion: Banana responded to the mild chilling stress dramatically at the molecular level. To compensate for the decreased photosynthesis efficiency caused by mild and moderate chilling stresses, banana accelerated its carbon fixation, mainly through upregulation of phosphoenolpyruvate carboxylases.

scholarly journals Auxin-transporting ABC transporters are defined by a conserved D/E-P motif regulated by a prolylisomerase

2020 ◽  
Vol 295 (37) ◽  
pp. 13094-13105 ◽  
Author(s):  
Pengchao Hao ◽  
Jian Xia ◽  
Jie Liu ◽  
Martin Di Donato ◽  
Konrad Pakula ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Plant Hormone ◽  
Auxin Transport ◽  
Physical Interaction ◽  
Transport Activity ◽  
Nucleotide Binding Domain ◽  
Higher Plant ◽  
Ppiase Activity ◽  
Auxin Distribution ◽  
A Cell

The plant hormone auxin must be transported throughout plants in a cell-to-cell manner to affect its various physiological functions. ABCB transporters are critical for this polar auxin distribution, but the regulatory mechanisms controlling their function is not fully understood. The auxin transport activity of ABCB1 was suggested to be regulated by a physical interaction with FKBP42/Twisted Dwarf1 (TWD1), a peptidylprolyl cis-trans isomerase (PPIase), but all attempts to demonstrate such a PPIase activity by TWD1 have failed so far. By using a structure-based approach, we identified several surface-exposed proline residues in the nucleotide binding domain and linker of Arabidopsis ABCB1, mutations of which do not alter ABCB1 protein stability or location but do affect its transport activity. P1008 is part of a conserved signature D/E-P motif that seems to be specific for auxin-transporting ABCBs, which we now refer to as ATAs. Mutation of the acidic residue also abolishes auxin transport activity by ABCB1. All higher plant ABCBs for which auxin transport has been conclusively proven carry this conserved motif, underlining its predictive potential. Introduction of this D/E-P motif into malate importer, ABCB14, increases both its malate and its background auxin transport activity, suggesting that this motif has an impact on transport capacity. The D/E-P1008 motif is also important for ABCB1-TWD1 interactions and activation of ABCB1-mediated auxin transport by TWD1. In summary, our data imply a new function for TWD1 acting as a putative activator of ABCB-mediated auxin transport by cis-trans isomerization of peptidyl-prolyl bonds.

scholarly journals Identification of candidate ATP-binding cassette transporter gene family members in Diaphorina citri (Hemiptera: Psyllidae) via adult tissues transcriptome analysis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Zhengbing Wang ◽  
Fajun Tian ◽  
Lijun Cai ◽  
Jie Zhang ◽  
Jiali Liu ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Expression Profiles ◽  
Atp Binding ◽  
Diaphorina Citri ◽  
Agricultural Pests ◽  
Adult Tissues ◽  
Living Organisms ◽  
Abc Transporter Genes ◽  
Upregulated Genes ◽  
Atp Binding Cassette

Abstract The ATP-binding cassette (ABC) transporters exist in all living organisms and play major roles in various biological functions by transporting a wide variety of substrates across membranes. The functions of ABC transporters in drug resistance have been extensively studied in vertebrates; however, they are rarely characterized in agricultural pests. The Asian citrus psyllid, Diaphorina citri, is one of the most damaging pests of the Citrus genus because of its transmission of Huanglongbing, also known as Yellow Dragon disease. In this study, the next-generation sequencing technique was applied to research the ABC transporters of D. citri. Fifty-three ABC transporter genes were found in the RNA-Seq data, and among these ABC transporters, 4, 4, 5, 2, 1, 4, 18 and 15 ABC proteins belonged to the ABCA-ABCH subfamilies, respectively. Different expression profiles of 52 genes between imidacloprid-resistant and imidacloprid-susceptible strains were studied by qRT-PCR; 5 ABCGs and 4 ABCHs were significantly upregulated in the imidacloprid-resistant strain. In addition, five of the nine upregulated genes were widely expressed in adult tissues in spatial expression analysis. The results suggest that these genes may play key roles in this phenotype. In general, this study contributed to our current understanding of D. citri resistance to insecticides.

scholarly journals Evidence for a Role of the Adenosine 5′-Triphosphate-Binding Cassette Transporter A1 in the Externalization of Annexin I from Pituitary Folliculo-Stellate Cells

Endocrinology ◽  
2003 ◽  
Vol 144 (3) ◽  
pp. 1062-1073 ◽  
Author(s):  
Lee P. Chapman ◽  
Matthew J. Epton ◽  
Julia C. Buckingham ◽  
John F. Morris ◽  
Helen C. Christian
Keyword(s):  
Abc Transporters ◽  
Anterior Pituitary ◽  
Signal Sequence ◽  
Atp Binding ◽  
Annexin I ◽  
Adenocarcinoma Cells ◽  
Inhibitory Feedback ◽  
Immunofluorescence Labeling ◽  
Atp Binding Cassette

Annexin 1 (ANXA1) has a well-demonstrated role in early delayed inhibitory feedback of glucocorticoids in the pituitary. ANXA1 is located in folliculo-stellate (FS) cells, and glucocorticoids act on these cells to externalize and stimulate the synthesis of ANXA1. However, ANXA1 lacks a signal sequence so the mechanism by which ANXA1 is externalized from FS cells was unknown and has been investigated. The ATP-binding cassette (ABC) transporters are a large group of transporters with varied roles that include the externalization of proteins. Glucocorticoid-induced externalization of ANXA1 from an FS cell line (TtT/GF) and rat anterior pituitary was blocked by glyburide, which inhibits ABC transporters. Glyburide also blocked the glucocorticoid inhibition of forskolin-stimulated ACTH release from pituitary tissue in vitro. RT-PCR revealed mRNA and Western blotting demonstrated protein for the ATP binding cassette A1 (ABCA1) transporter in mouse FS, TtT/GF, and A549 lung adenocarcinoma cells from which glucocorticoids also induce externalization of ANXA1. In TtT/GF cells, immunofluorescence labeling revealed a near total colocalization of cell surface ANXA1 and ABCA1. We conclude that ANXA1, which mediates the early delayed feedback of glucocorticoids in the anterior pituitary, is externalized from FS cells by an ABC transporter and that the ABCA1 transporter is a likely candidate.

scholarly journals Regulation von ABC-Transportern durch FKBPs

BIOspektrum ◽  
2021 ◽  
Vol 27 (2) ◽  
pp. 131-134
Author(s):  
Markus Geisler
Keyword(s):  
Abc Transporter ◽  
Abc Transporters ◽  
Plant Hormone ◽  
Auxin Transport ◽  
Current Knowledge ◽  
Binding Protein ◽  
Atp Binding ◽  
Fk506 Binding Protein ◽  
Atp Binding Cassette

AbstractThe plant hormone auxin is distributed in the plant by a sophisticated network of importers and exporters, including members of the ABCB subclass of ATP-binding cassette (ABC) transporters. ABCB-mediated auxin transport is controlled by Twisted Dwarf1, a member of the FK506-binding protein (FKBP) family. Here, we summarize current knowledge on ABC transporter regulation by FKBPs, which seems to be conserved over kingdoms and ABC subfamilies arguing for conserved mechanism of plant and mammalian post-translational transporter regulation.

scholarly journals Differentially expressed genes and phenotypic variation in florets among the isogenic Ogura-CMS, DGMS and inbred lines of broccoli

2020 ◽  
Author(s):  
Zhansheng Li ◽  
Yumei Liu ◽  
Zhiyuan Fang ◽  
Limei Yang ◽  
Mu Zhuang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Male Sterility ◽  
Seed Yield ◽  
Inbred Line ◽  
Abc Transporters ◽  
Plant Hormone ◽  
Inbred Lines ◽  
Maintainer Line ◽  
Late Flowering ◽  
Cms Line

Abstract Background: Male sterility contributes strongly to hybrid seed purity and production in Brassica crops. To detect plant phenotypes and the gene expression patterns involved in bioprocess of an Ogura cytoplasmic male sterility line (Ogura-CMS), a dominant genic male sterility line (DGMS) and their maintainer line, we analyzed the transcriptomes of broccoli florets among the isogenic Ogura-CMS (T54C), DGMS (T54M) and inbred lines (T54S).Results: There were respectively 505, 585 and 469 upregulated genes, and 1109, 1073 and 543 downregulated genes in the comparison groups of T54C and T54M, T54C and T54S, and T54M and T54S. The head weight and head width showed stronger performance in the Ogura-CMS than the DGMS line or maintainer line. The Ogura-CMS line showed poorer performance in seed yield and seed germination than the DGMS line or maintainer line. The DGMS line had longer maturation and flowering periods than the Ogura-CMS and maintainer lines. The plant hormone genes, auxin (TIR1, GH3 and SAUR) and salicylic acid (SA) (NPR1), and adenosine triphosphate-binding cassette (ABC) transporters-related genes (ABCB1 and ATM) were highly up-regulated in T54C compared with T54M or T54S. The brassinosteroid upregulated gene CYCD3 related to the function of late-flowering and delaying senescence in plants, was detected in T54M over two seasons.Conclusions: Among the isogenic Ogura-CMS, DGMS and inbred lines of broccoli, the Ogura-CMS line showed strong performance in head yield than the DGMS line or the inbred line. The DGMS line had longer days to flowering than the Ogura-CMS and inbred lines. However, the inbred line presented a higher seed yield and seed germination rate than the DGMS or Ogura-CMS line. This study found that some potential plant hormone genes, auxin (TIR1, GH3 and SAUR) and SA (NPR1), and ABC transporters related genes (ABCB1 and ATM) might play a key role in regulation of the developmental trait in the Ogura-CMS line. The BR- upregulated gene CYCD3 may function in late-flowering and delaying senescence of broccoli consistent with the investigations conducted over two seasons. Our findings provided a possible explanation for physiological and developmental differences of broccoli among the Ogura-CMS, the DGMS and the inbred lines.

scholarly journals Bitki Mikrop Etkileşiminin Sekonder Metabolitler Üzerindeki Etkisi

2021 ◽  
Vol 9 (2) ◽  
pp. 281-287
Author(s):  
Mehmet Veysi Çetiz ◽  
Abdulrezzak Memon
Keyword(s):  
Secondary Metabolites ◽  
Host Plant ◽  
Significant Role ◽  
Plant Hormone ◽  
Molecular Level ◽  
Current Knowledge ◽  
Review Article ◽  
Essential Nutrients ◽  
Plant Microbe Interaction ◽  
Plant Microbiome

The microbes that live in and on plants (the plant microbiome) are critical for plant health and exert their influence by facilitating the nutrient acquisition, regulating plant hormone levels, and helping to withstand pathogen attack. Plants are meta-organisms that are associated with complex microbiomes. The majority of the microorgansims including epiphytes and endophytes generally play a significant role in providing essential nutrients to the plants where they live. In addition, plant microbe interaction affects the content of secondary metabolites and their derivatives in the host plant. In this review article we summarizes the interaction of the plant and microbe interaction especially the microorganisms of the rhizosphere and their effect on the secondary metabolites level in plants. The current knowledge of the plant- microbe interaction at molecular level are also being reviewed in brief.

scholarly journals Rapid Auxin-Mediated Cell Expansion

2020 ◽  
Vol 71 (1) ◽  
pp. 379-402 ◽  
Author(s):  
Minmin Du ◽  
Edgar P. Spalding ◽  
William M. Gray
Keyword(s):  
Plasma Membrane ◽  
Cell Expansion ◽  
Plant Hormone ◽  
Molecular Level ◽  
Good News ◽  
Growth Theory ◽  
Acid Growth ◽  
Promotive Effect ◽  
Acid Growth Theory ◽  
Regulate Protein

The promotive effect of auxin on shoot cell expansion provided the bioassay used to isolate this central plant hormone nearly a century ago. While the mechanisms underlying auxin perception and signaling to regulate transcription have largely been elucidated, how auxin controls cell expansion is only now attaining molecular-level definition. The good news is that the decades-old acid growth theory invoking plasma membrane H+-ATPase activation is still useful. The better news is that a mechanistic framework has emerged, wherein Small Auxin Up RNA (SAUR) proteins regulate protein phosphatases to control H+-ATPase activity. In this review, we focus on rapid auxin effects, their relationship to H+-ATPase activation and other transporters, and dependence on TIR1/AFB signaling. We also discuss how some observations, such as near-instantaneous effects on ion transport and root growth, do not fit into a single, comprehensive explanation of how auxin controls cell expansion, and where more research is warranted.

Interactions of PIN and PGP auxin transport mechanisms

2007 ◽  
Vol 35 (1) ◽  
pp. 137-141 ◽  
Author(s):  
A. Bandyopadhyay ◽  
J.J. Blakeslee ◽  
O.R. Lee ◽  
J. Mravec ◽  
M. Sauer ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Plant Hormone ◽  
Auxin Transport ◽  
Protein Complexes ◽  
Transport Mechanisms ◽  
In Planta ◽  
P Glycoprotein ◽  
Polarized Transport ◽  
Major Facilitator ◽  
And Function

Polarized transport of the plant hormone auxin influences multiple growth processes in plants and is regulated by plasma-membrane-localized efflux and uptake carriers. The PGP (P-glycoprotein) ABC transporters (ATP-binding-cassette transporters), PIN (pin-formed) subfamily of major facilitator proteins and members of AUX/LAX families have been shown to independently transport auxin both in planta and in heterologous systems. However, PIN- and PGP-mediated transport in heterologous systems exhibits decreased substrate specificity and inhibitor-sensitivity compared with what is seen in plants and plant cells. To determine whether PIN–PGP interactions enhance transport specificity, we analysed interactions of the representative auxin-transporting PGPs with PIN1 and AUX1 in planta and in heterologous systems. Here, we provide evidence that PINs and PGPs interact and function both independently and co-ordinately to control polar auxin transport and impart transport specificity and directionality. These interactions take place in protein complexes stabilized by PGPs in detergent-resistant microdomains.

scholarly journals Transcriptome Profiling Unveils GAP43 Regulates ABC Transporters and EIF2 Signaling in Colorectal Cancer Cells

2020 ◽  
Author(s):  
Xi Chen ◽  
Hongjin Wu ◽  
Li Xiao ◽  
Jia Feng ◽  
Danmeng Sun ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Signaling Pathway ◽  
Abc Transporters ◽  
Transcriptome Profiling ◽  
Histone Deacetylation ◽  
Receptor Interaction ◽  
Functional Roles ◽  
Transcriptional Alterations ◽  
And Function ◽  
Upregulated Genes

Abstract BackgroundThe growth- and plasticity-associated protein-43 (GAP43) is biasedly expressed in indigestive system and nervous system. Recent study has shown that GAP43 is responsible for the development of neuronal growth and axonal regeneration in normal nervous tissue, while serves as a specific biomarker of relapsed or refractory neuroblastoma. However, its expression pattern and function in digestive system cancer still remains to be clarified.ResultsIn this study, we found GAP43 was downregulated in colorectal cancer (CRC) compared to the adjacent tissues. DNA methylase inhibitor 5-Aza-CdR treatment could significantly induce GAP43, indicated that the silencing of GAP43 gene in CRC is closely related to DNA methylation and histone deacetylation. Bisulfite genomic sequencing confirmed the promoter methylation of GAP43 in CRC. To explore the transcriptional alterations by overexpressed GAP43 in CRC, we performed RNA-seq and found that upregulated genes were significantly enriched in the signaling pathways of ABC transporters and ECM-receptor interaction, while downregulated genes were significantly enriched in Ribosome signaling pathway. Further Ingenuity Pathway Analysis (IPA) showed that EIF2 signaling pathway was significantly repressed by overexpression of GAP43.ConclusionOur findings provide a novel mechanistic insight of GAP43 in CRC. Transcriptome profiling of overexpressed GAP43 in CRC uncovered the functional roles of GAP43 in the development of human CRC.

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