scholarly journals Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate Amphidinium carterae (Subclade III)

2021 ◽  
Vol 22 (22) ◽  
pp. 12196
Author(s):  
Maria Elena Barone ◽  
Elliot Murphy ◽  
Rachel Parkes ◽  
Gerard T. A. Fleming ◽  
Floriana Campanile ◽  
...  
Keyword(s):  
Solid Phase ◽  
Phylogenetic Inference ◽  
Bioactive Metabolites ◽  
The West ◽  
Fractionation Process ◽  
Amphidinium Carterae ◽  
Biological Assays ◽  
Marine Dinoflagellate ◽  
Stage 1 ◽  
Metabolomic Approach

Microalgae have received growing interest for their capacity to produce bioactive metabolites. This study aimed at characterising the antimicrobial potential of the marine dinoflagellate Amphidinium carterae strain LACW11, isolated from the west of Ireland. Amphidinolides have been identified as cytotoxic polyoxygenated polyketides produced by several Amphidinium species. Phylogenetic inference assigned our strain to Amphidinium carterae subclade III, along with isolates interspersed in different geographic regions. A two-stage extraction and fractionation process of the biomass was carried out. Extracts obtained after stage-1 were tested for bioactivity against bacterial ATCC strains of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa. The stage-2 solid phase extraction provided 16 fractions, which were tested against S. aureus and E. faecalis. Fractions I, J and K yielded minimum inhibitory concentrations between 16 μg/mL and 256 μg/mL for both Gram-positive. A targeted metabolomic approach using UHPLC-HRMS/MS analysis applied on fractions G to J evidenced the presence of amphidinol type compounds AM-A, AM-B, AM-22 and a new derivative dehydroAM-A, with characteristic masses of m/z 1361, 1463, 1667 and 1343, respectively. Combining the results of the biological assays with the targeted metabolomic approach, we could conclude that AM-A and the new derivative dehydroAM-A are responsible for the detected antimicrobial bioactivity.

scholarly journals A Cross-Metabolomic Approach Shows that Wheat Interferes with Fluorescent Pseudomonas Physiology through Its Root Metabolites

Metabolites ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 84
Author(s):  
Laura Rieusset ◽  
Marjolaine Rey ◽  
Florence Gerin ◽  
Florence Wisniewski-Dyé ◽  
Claire Prigent-Combaret ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Host Plant ◽  
Soil Microorganisms ◽  
Wheat Root ◽  
Bioactive Metabolites ◽  
Root Extracts ◽  
Fluorescent Pseudomonas ◽  
Health And Development ◽  
The Impact ◽  
Metabolomic Approach

Roots contain a wide variety of secondary metabolites. Some of them are exudated in the rhizosphere, where they are able to attract and/or control a large diversity of microbial species. In return, the rhizomicrobiota can promote plant health and development. Some rhizobacteria belonging to the Pseudomonas genus are known to produce a wide diversity of secondary metabolites that can exert a biological activity on the host plant and on other soil microorganisms. Nevertheless, the impact of the host plant on the production of bioactive metabolites by Pseudomonas is still poorly understood. To characterize the impact of plants on the secondary metabolism of Pseudomonas, a cross-metabolomic approach has been developed. Five different fluorescent Pseudomonas strains were thus cultivated in the presence of a low concentration of wheat root extracts recovered from three wheat genotypes. Analysis of our metabolomic workflow revealed that the production of several Pseudomonas secondary metabolites was significantly modulated when bacteria were cultivated with root extracts, including metabolites involved in plant-beneficial properties.

scholarly journals Characterization of the Volatile Profile of Cultivated and Wild-Type Italian Celery (Apium graveolens L.) Varieties by HS-SPME/GC-MS

2021 ◽  
Vol 11 (13) ◽  
pp. 5855
Author(s):  
Samantha Reale ◽  
Valter Di Cecco ◽  
Francesca Di Donato ◽  
Luciano Di Martino ◽  
Aurelio Manzi ◽  
...  
Keyword(s):  
Solid Phase ◽  
Principal Component ◽  
Apium Graveolens ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Metabolites ◽  
Chemical Components ◽  
Volatile Profile ◽  
Wild Type ◽  
Multivariate Statistical ◽  
Clear Differentiation

Celery (Apium graveolens L.) is a vegetable belonging to the Apiaceae family that is widely used for its distinct flavor and contains a variety of bioactive metabolites with healthy properties. Some celery ecotypes cultivated in specific territories of Italy have recently attracted the attention of consumers and scientists because of their peculiar sensorial and nutritional properties. In this work, the volatile profiles of white celery “Sedano Bianco di Sperlonga” Protected Geographical Indication (PGI) ecotype, black celery “Sedano Nero di Torricella Peligna” and wild-type celery were investigated using head-space solid-phase microextraction combined with gas-chromatography/mass spectrometry (HS-SPME/GC-MS) and compared to that of the common ribbed celery. Exploratory multivariate statistical analyses were conducted using principal component analysis (PCA) on HS-SPME/GC-MS patterns, separately collected from celery leaves and petioles, to assess similarity/dissimilarity in the flavor composition of the investigated varieties. PCA revealed a clear differentiation of wild-type celery from the cultivated varieties. Among the cultivated varieties, black celery “Sedano Nero di Torricella Peligna” exhibited a significantly different composition in volatile profile in both leaves and petioles compared to the white celery and the prevalent commercial variety. The chemical components of aroma, potentially useful for the classification of celery according to the variety/origin, were identified.

scholarly journals Effect of 2CaO·SiO2 particles addition on dephosphorization behavior

2020 ◽  
Vol 39 (1) ◽  
pp. 219-227
Author(s):  
Aijun Deng ◽  
Yunjin Xia ◽  
Jie Li ◽  
Dingdong Fan
Keyword(s):  
Liquid Slag ◽  
Solid Phase ◽  
Solid Particles ◽  
Hot Metal ◽  
Liquid Silicate ◽  
Phase Complex ◽  
Dephosphorization Slag ◽  
Stage 1 ◽  
Two Stages ◽  
Complex Liquid

AbstractThe effect of the addition of 2CaO·SiO2 solid particles on dephosphorization behavior in carbon-saturated hot metal was investigated. The research results showed that the addition of 2CaO·SiO2 particles have little influence on desilication and demanganization, and the removal of [Si] and [Mn] occurred in the first 5 min with different conditions where the contents of 2CaO·SiO2 particles addition for the conditions 1, 2, 3, 4, and 5 are 0, 2.2, 6.4, 8.6, and 13.0 g, respectively. The final dephosphorization ratios for the conditions 1, 2, 3, 4, and 5 are 61.2%, 66.9%, 79.6%, 63.0%, and 78.1%, respectively. The dephosphorization ratio decreases with the increase of 2CaO·SiO2 particles in the first 3 min. The reason for this is that the dephosphorization process between hot metal and slag containing C2S phase consisted of two stages: Stage 1, [P] transfers from hot metal to liquid slag and Stage 2, the dephosphorization production (3CaO·P2O5) in liquid slag reacts with 2CaO·SiO2 to form C2S–C3P solid solution. The increase of 2CaO·SiO2 particles increases the viscosity of slag and weakens the dephosphorization ability of the stage 1. The SEM and XRD analyses show that the phase of dephosphorization slag with the addition of different 2CaO·SiO2 particles is composed of white RO phase, complex liquid silicate phase, and black solid phase (C2S or C2S–C3P). Because the contents of C2S–C3P and 2CaO·SiO2 in slag and the dephosphorization ability of the two stages are different, the dephosphorization ability with different conditions is different.

Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae

2020 ◽  
Vol 313 ◽  
pp. 123518
Author(s):  
M. López-Rodríguez ◽  
M.C. Cerón-García ◽  
L. López-Rosales ◽  
E. Navarro-López ◽  
A. Sánchez-Mirón ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Amphidinium Carterae ◽  
Marine Dinoflagellate

scholarly journals A New Unsaturated Glycoglycerolipid from a Cultured Marine Dinoflagellate Amphidinium carterae

2005 ◽  
Vol 53 (3) ◽  
pp. 330-332 ◽  
Author(s):  
Jun Wu ◽  
Lijuan Long ◽  
Yang Song ◽  
Si Zhang ◽  
Qingxin Li ◽  
...  
Keyword(s):  
Amphidinium Carterae ◽  
Marine Dinoflagellate

Some properties of a fluoride-resistant mutant of the marine dinoflagellate Amphidinium carterae

Phycologia ◽  
1984 ◽  
Vol 23 (3) ◽  
pp. 301-310 ◽  
Author(s):  
M. Emilia Klut ◽  
Naval J. Antia ◽  
Thana Bisalputra
Keyword(s):  
Resistant Mutant ◽  
Amphidinium Carterae ◽  
Marine Dinoflagellate

Long-term culture of the marine dinoflagellate microalga Amphidinium carterae in an indoor LED-lighted raceway photobioreactor: Production of carotenoids and fatty acids

2018 ◽  
Vol 265 ◽  
pp. 257-267 ◽  
Author(s):  
A. Molina-Miras ◽  
L. López-Rosales ◽  
A. Sánchez-Mirón ◽  
M.C. Cerón-García ◽  
S. Seoane-Parra ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Amphidinium Carterae ◽  
Long Term Culture ◽  
Marine Dinoflagellate

scholarly journals Shift of Aromatic Profile in Probiotic Hemp Drink Formulations: A Metabolomic Approach

2019 ◽  
Vol 7 (11) ◽  
pp. 509 ◽  
Author(s):  
Nissen ◽  
Demircan ◽  
Taneyo-Saa ◽  
Gianotti
Keyword(s):  
Solid Phase ◽  
Industrial Applications ◽  
Bifidobacterium Bifidum ◽  
Gas Chromatography Mass Spectrometry ◽  
Hemp Seed ◽  
Nutritional Values ◽  
Novel Approach ◽  
Milk Substitutes ◽  
Metabolomic Approach

Vegetal drinks as a substitute for milk consumption are raising striking interest in the food industry. Soy and rice drinks are the most successful milk substitutes but are low in protein and fiber contents, are rich in sugars, and their cultivation systems are unsustainable; thus, alternative vegetal sources to resolve these limits must be found. A winning candidate could be hemp seed, which is a powerhouse of nutrients, is sugarless, rich in fiber and proteins, and little land and nutrients demanding. The aim is to develop novel drinks obtained from hemp seeds mixed or not with soy and rice and fermented with probiotics (Lactobacillus fermentum, Lb. plantarum, and Bifidobacterium bifidum). The drinks were characterized for their microbial growth, by means of culture-dependent and -independent techniques, and for their volatilome, by means of solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis. The results showed that hemp seed drinks have a specific aroma and its compounds are dependent on the type of formulation and to the probiotic used. For example, in hemp seed drinks, 2-heptanol, 2-methyl, 2,4-decadienal, 2-butanone, 3-hydroxy, 2,3-butanedione, and propanoic acid were fine descriptors of probiotics fermentations. Multivariate analysis of volatile metabolites and their correlation to some physiological parameters and nutritional values offered a novel approach to assess the quality of functional hemp drinks which could result in a decisional tool for industrial applications.

scholarly journals Colonic In Vitro Model Assessment of the Prebiotic Potential of Bread Fortified with Polyphenols Rich Olive Fiber

Nutrients ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 787
Author(s):  
Lorenzo Nissen ◽  
Flavia Casciano ◽  
Elena Chiarello ◽  
Mattia Di Nunzio ◽  
Alessandra Bordoni ◽  
...  
Keyword(s):  
Solid Phase ◽  
Bacterial Species ◽  
Critical Evaluation ◽  
Value Added ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Metabolites ◽  
Model Assessment ◽  
Olive Pomace ◽  
Positive Effects

The use of olive pomace could represent an innovative and low-cost strategy to formulate healthier and value-added foods, and bakery products are good candidates for enrichment. In this work, we explored the prebiotic potential of bread enriched with Polyphenol Rich Fiber (PRF), a defatted olive pomace byproduct previously studied in the European Project H2020 EcoProlive. To this aim, after in vitro digestion, the PRF-enriched bread, its standard control, and fructo-oligosaccharides (FOS) underwent distal colonic fermentation using the in vitro colon model MICODE (multi-unit colon gut model). Sampling was done prior, over and after 24 h of fermentation, then metabolomic analysis by Solid Phase Micro Extraction Gas Chromatography Mass Spectrometry (SPME GCMS), 16S-rDNA genomic sequencing of colonic microbiota by MiSeq, and absolute quantification of main bacterial species by qPCR were performed. The results indicated that PRF-enriched bread generated positive effects on the host gut model: (i) surge in eubiosis; (ii) increased abundance of beneficial bacterial groups, such as Bifidobacteriaceae and Lactobacillales; (iii) production of certain bioactive metabolites, such as low organic fatty acids; (iv) reduction in detrimental compounds, such as skatole. Our study not only evidenced the prebiotic role of PRF-enriched bread, thereby paving the road for further use of olive by-products, but also highlighted the potential of the in vitro gut model MICODE in the critical evaluation of functionality of food prototypes as modulators of the gut microbiota.

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