scholarly journals Separation and Identification of Resveratrol Butyrate Ester Complexes and Their Bioactivity in HepG2 Cell Models

2021 ◽  
Vol 22 (24) ◽  
pp. 13539
Author(s):  
Ming-Kuei Shih ◽  
You-Lin Tain ◽  
Chiu-Min Cheng ◽  
Chien-Ning Hsu ◽  
Yu-Wei Chen ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Silica Gel ◽  
High Performance ◽  
Antioxidant Activities ◽  
Cell Models ◽  
Practical Application ◽  
Cell Survival Rate ◽  
Successful Isolation ◽  
Key Characteristics ◽  
1H Nuclear Magnetic Resonance

Resveratrol butyrate ester (RBE) complexes have demonstrated higher antioxidant capacity and anti-fat accumulation activity in previous studies. In this study, silica gel, high-performance liquid chromatography, and 1H nuclear magnetic resonance were used for separation and identification of RBE complex components. With the exception of resveratrol, five different structures of ester derivatives were separated from silica gel: 3,4′-di-O-butanoylresveratrol (ED2, 18.8%), 3-O-butanoylresveratrol (ED4, 35.7%), 4′-O-butanoylresveratrol (ED5, 4.4%), 3,5,4′-tri-O-butanoylresveratrol (ED6, 1.5%), and 3,5-di-O-butanoylresveratrol (ED7, 0.7%). Among the ester derivatives obtained, ED2 and ED4 were the main ester derivatives in the RBE complex. Thus, the cellular antioxidant activities of the RBE mixture, ED2, and ED4 were evaluated. Results showed that the antioxidant capacity of ED2 and ED4 was higher than that of the RBE mixture, demonstrating that the number and position of butyrate esterification sites are related to cell survival rate and antioxidant capacity. This study is the first to report the successful isolation, structural identification, and cellular biological antioxidant activity of RBE complex derivatives, which are key characteristics for the potential practical application of RBE complexes.

scholarly journals PHYTOCHEMICAL CONSTITUENTS, PHENOLIC CONTENTS, AND ANTIOXIDANT ACTIVITY OF CRATAEGUS AZAROLUS EXTRACTS

2018 ◽  
Vol 11 (4) ◽  
pp. 133 ◽  
Author(s):  
Boudjada Amina ◽  
Touil Ahmed ◽  
Bendif Hamdi ◽  
Bensouici Chawki ◽  
Rhouati Salah
Keyword(s):  
Antioxidant Activity ◽  
Volatile Compounds ◽  
Silica Gel ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Isolation And Identification ◽  
Phenolic Contents ◽  
Butanol Extract ◽  
Chcl3 Extract ◽  
1H Nuclear Magnetic Resonance

 Objective: The aim of this study was the isolation and identification of secondary metabolites from Crataegus azarolus (L.) and the antioxidant evaluation of its extracts and compounds.Methods: The air-dried powdered parts of the plant were extracted with 70% methanol and fractionated by chloroform, ethyl acetate, and n-butanol. The n-butanol extract was separated using polyamide SC6 column and silica gel TLC. In addition, a fraction of silica gel column of the CHCl3 extract was analyzed by gas chromatography–mass spectrometer (GC–MS). The total phenolic and total flavonoid contents of CHCl3 and n-butanol extracts were estimated. Furthermore, the antioxidant activities of CHCl3, n-butanol extracts, and two flavonoids were evaluated according to five different methods.Results: Eight compounds were identified in CHCl3 and n-butanol extracts, among them, five volatile compounds identified by GC–MS for the 1st time from the species, as well as three known flavonol glycosides identified by spectral analysis (ultraviolet,1H-nuclear magnetic resonance [NMR], and13C-NMR) and by comparison with literature data. The n-butanol extract showed the higher content of polyphenols (307.33 ± 2.33 mg (gallic acid equivalents)/g extract) and flavonoids (143.0 ± 2.12 mg QE/g extract) and it proves the highest antioxidant activity with all assays used.Conclusion: Five volatile compounds were identified for the 1st time from the C. azarolus and the antioxidant potential of plant extracts was measured using five different methods.

Phenolic composition and antioxidant capacity of hawthorn (Crataegus oxyacantha L.) flowers and fruits grown in Algeria

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ouarda Issaadi ◽  
Marta Fibiani ◽  
Valentina Picchi ◽  
Roberto Lo Scalzo ◽  
Khodir Madani
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Paramagnetic Resonance ◽  
Array Detection ◽  
The Relationship

AbstractBackgroundTo the best of our knowledge, up to now, there are no reports on the antioxidant activity of phenolic compounds of Crataegus oxyacantha flowers and fruits. Such detailed information is essential to advance the existing knowledge and to promote the use of this species growing in Africa.Therefore, the objective of this investigation was to analyze the content of phenolic compounds as well as the antioxidant activities of flowers and fruits of C. oxyacantha from Bejaia (Northeastern Algeria) by different analytical methods, and to determine the relationship between them.MethodsPhenolic compounds and antioxidant activity of Algerian hawthorn flowers and fruits (C. oxyacantha L.) were studied. Total phenolics, flavonoids and flavonols were determined using colorimetric methods. The phenolic profile was analyzed by high-performance liquid chromatography with diode array detection, and the in vitro antioxidant activity was measured using scavenging assay (FRAP) and Fremy’s salt, using electronic paramagnetic resonance.ResultsDifferent classes of phenolic compounds were identified and quantified. Hawthorn flowers contained higher levels of hydroxycinnamic acids, vitexin derivatives and flavonols compared to fruits, while anthocyanins were present only in fruits. Significant correlations were found between phenolic content and antioxidant activity. The results also showed that although the phenolic content of the two parts was different, their antioxidant capacity was not statistically different.ConclusionsThe results of this study indicate that hawthorn flowers and fruits of C. oxyacantha may be considered as a natural source of bioactive compounds.

scholarly journals Synergies of herbal teas on improved plasma antioxidant capacity and postprandial hypoglycemic response in healthy adults

2021 ◽  
Vol 13 ◽  
Author(s):  
Anoma Chandrasekara ◽  
Apeksha Herath ◽  
Rupika Abeynayake ◽  
Adriano Costa De Camargo ◽  
Fereidoon Shahidi
Keyword(s):  
Antioxidant Capacity ◽  
High Performance ◽  
Antioxidant Activities ◽  
Healthy Adults ◽  
Herbal Tea ◽  
Plasma Antioxidant Capacity ◽  
Liquid Chromatography Tandem Mass ◽  
Food Cultures ◽  
Plasma Antioxidant ◽  
Glucose Reduction

Herbal teas play a major role in the global traditional food cultures.  There are a variety of herbal teas used for general wellness and prevention and management of chronic diseases including diabetes mellitus. The present study aimed to determine the phenolic content and antioxidant activities of herbal teas prepared with matured jackfruit (Artocarpus heterophyllus) leaves after senescence and abscission, and dried Cassia (Cassia auriculata) flowers and buds.  The sensory evaluation was performed using 50 untrained panelists to determine the amount of herbal ingredient to be used in the preparation of herbal tea. Furthermore, the potential single dose efficacy of three herbal teas, namely, Jackfruit leaves tea (JLT), Cassia flower tea (CFT) and mixed jackfruit leaves and Cassia flower tea (JCT) on postprandial glycemic response and plasma antioxidant capacity (PAC) of healthy adults were investigated. Phenolics were identified using high performance liquid chromatography–tandem mass spectrometry. A randomized crossover study was led with 16 healthy adults who consumed 250 mL of freshly prepared herbal beverage with 50 g glucose and 50 g glucose in 250 mL water as the control randomly within three visits with a washout period of four days between two visits. Blood samples were collected using microcapillary tubes at the baseline and postprandial at 30, 45, 60, 90, and 120 min. The plasma was analyzed for glucose concentration (PGC) and PAC.  JLT and JCT showed a significant reduction of PGC compared to the control at 30, 45, and 60 min time points.  JLT and JCT showed higher efficacy in plasma glucose reduction than CFT.  All herbal teas significantly increased PAC at the end of 120 min post ingestion.  Further research is warranted to examine the synergistic effect of two mixed ingredients for herbal tea and the long-term efficacy of multiple dose ingestion of respective herbal teas in the prevention and management of hyperglycemia and type 2 diabetes.

scholarly journals Screening and Characterization of Phenolic Compounds from Australian Grown Bananas and Their Antioxidant Capacity

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1521
Author(s):  
Yasmeen M. Bashmil ◽  
Akhtar Ali ◽  
Amrit BK ◽  
Frank R. Dunshea ◽  
Hafiz A. R. Suleria
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Antioxidant Activities ◽  
Banana Peel ◽  
Array Detector ◽  
Total Polyphenols ◽  
Photodiode Array Detector ◽  
Photodiode Array

Bananas are an essential source of staple food and fruit worldwide and are widely regarded as the world’s largest fruit crop, with more than 100 million tons total annual production. Banana peel, a by-product that represents about 40% of the entire banana’s weight, and pulp are rich in bioactive compounds and have a high antioxidant capacity. As the production of polyphenols in fruit and vegetables is highly dependent on environmental conditions, genetic factors, and the level of maturity, this study aims to characterize six Australian banana cultivars in various stages of ripening for their phenolic compounds using the liquid chromatography-electrospray ionization quadrupole time of flight mass spectrometry (LC-ESI-QTOF-MS/MS), polyphenols quantification with the high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA), and their antioxidant capacity. All bananas were analysed for total polyphenols content (TPC), total flavonoids content (TFC), and total tannin content (TTC) and their antioxidant activities. Ripe Ducasse peel and pulp contained the highest amounts of total polyphenols content (1.32 and 1.28 mg gallic acid equivalent (GAE) per gram of sample), total tannin contents (3.34 mg catechin equivalent (CE) per gram of sample), and free radical scavenging capacity (106.67 mg ascorbic acid equivalent (AAE) per g of sample). In contrast, ripe Plantain peel had the greatest total flavonoids (0.03 mg quercetin equivalent (QE) per g of sample). On the other hand, unripe Ladyfinger pulp possessed the highest total antioxidant activity (1.03 mg AAE/g of sample). There was a positive correlation between flavonoids and antioxidant activities. By using LC-ESI-QTOF-MS/MS, a total of 24 phenolic compounds were tentatively characterized in this research, including six phenolic acids, 13 flavonoids, and five other polyphenols. Quantification of phenolic compounds by the high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA) revealed a higher content of phenolic acids. These findings confirmed that banana peel and pulp have considerable antioxidant activity and can be employed in human food and animal feed for variant health enhancement uses.

scholarly journals Bioactive compounds and quality characteristics of five apples cultivars

2015 ◽  
Vol 45 (11) ◽  
pp. 1972-1979 ◽  
Author(s):  
Moises Zucoloto ◽  
Kang-Mo Ku ◽  
Mosbah M. Kushad ◽  
Jamal Sawwan
Keyword(s):  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Bioactive Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Quality Characteristics ◽  
Total Phenolic ◽  
Liquid Chromatograph ◽  
Antioxidant Power

The aim of this study was to evaluate bioactive compounds in five apple cultivars and to analyze correlation of their quality characteristics with concentration of bioactive compounds. Phenolic compounds measurements were made in a spectrophotometer compared to a standard curve of gallic acid and expressed as gallic acid equivalent (GAE) per 100g of dry weight. Sugar and organics acids in five cultivars were quantified using high-performance liquid chromatograph (HPLC). Antioxidant activities were evaluated using three complementary tests 2,2-diphenyl-1-picrylhidrazyl (DPPH) scavenging activity, ferric reducing antioxidant power (FRAP), and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). 'GoldRush' had the highest total phenolic compared to the other four cultivars. Additionally, 'GoldRush' had slightly higher, DPPH activity followed by 'Crimson Crisp' and 'Wine Crisp'. 'GoldRush' and 'Crimson Crisp' cultivars also have higher antioxidant capacity based on the ABTS and FRAP methods. The antioxidant capacity was significantly correlated with total polyphenols present in the different cultivars, while organic acids and fruit color showed slightly significant correlation to total phenols

scholarly journals Optimization of sunflower head pectin extraction by ammonium oxalate and the effect of drying conditions on properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xuemei Ma ◽  
Jiayi Yu ◽  
Jing Jing ◽  
Qian Zhao ◽  
Liyong Ren ◽  
...  
Keyword(s):  
High Performance ◽  
Freeze Drying ◽  
Antioxidant Activities ◽  
Structural Characteristics ◽  
Radical Scavenging ◽  
Ammonium Oxalate ◽  
Extraction Process ◽  
Size Exclusion ◽  
Pharmaceutical Industries

AbstractPectin is a kind of natural and complex carbohydrates which is extensively used in food, chemical, cosmetic, and pharmaceutical industries. Fresh sunflower (Helianthus annuus L.) heads were utilized as a novel source of pectin extracted by ammonium oxalate. The conditions of the extraction process were optimized implementing the response surface methodology. Under optimal extraction parameters (extraction time 1.34 h, liquid–solid ratio 15:1 mL/g, ammonium oxalate concentration 0.76% (w/v)), the maximum experimental yield was 7.36%. The effect of spray-drying and freeze-drying on the physiochemical properties, structural characteristics, and antioxidant activities was investigated by FT-IR spectroscopy, high performance size exclusion chromatography, and X-ray diffraction. The results showed freeze-drying lead to decrease in galacturonic acid (GalA) content (76.2%), molecular weight (Mw 316 kDa), and crystallinity. The antioxidant activities of pectin were investigated utilizing the in-vitro DPPH and ABTS radical-scavenging systems. This study provided a novel and efficient extraction method of sunflower pectin, and confirmed that different drying processes had an effect on the structure and properties of pectin.

Thermal properties of polybenzoxazine exhibiting improved toughness: Blending with cyclodextrin and its derivatives

2021 ◽  
pp. 095400832110130
Author(s):  
Hailong Li ◽  
Sipei Zhao ◽  
Li Pei ◽  
Zihe Qiao ◽  
Ding Han ◽  
...  
Keyword(s):  
Thermal Properties ◽  
Hydrogen Bonds ◽  
High Performance ◽  
Practical Application ◽  
Scanning Calorimetry ◽  
Thermoset Resins ◽  
Chemical Structures ◽  
Microscopy Techniques ◽  
Fractured Surface ◽  
Scanning Electron

Polybenzoxazines are emerging as a class of high-performance thermoset polymers that can find their applications in various fields. However, its practical application is limited by its low toughness. The cyclic β-cyclodextrin and a newly synthesized derivative (β-cyclodextrin-MAH) were separately blended with benzoxazine to improve the toughness of polybenzoxazine. The results revealed that the maximum impact strength of the blend was 12.24 kJ·m−2 and 14.29 kJ·m−2 when 1 wt.% of β-Cyclodextrin and β-Cyclodextrin-MAH, respectively, were used. The strengths were 53% and 86% higher than that of pure polybenzoxazine. The curing reaction, possible chemical structures, and fractured surface were examined using differential scanning calorimetry, Fourier transform infrared spectroscopy, and scanning electron microscopy techniques to understand the mechanism of generation of toughness. The results revealed that the sea-island structure and the presence of hydrogen bonds between polybenzoxazine and β-cyclodextrin and β-cyclodextrin-MAH resulted in the generation of toughness. Furthermore, the curves generated during thermogravimetric analysis did not significantly change, revealing the good thermal properties of the system. The phase-separated structure and the hydrogen bonds present in the system can be exploited to prepare synergistically tough polybenzoxazine exhibiting excellent thermal properties. This can be a potential way of modifying the thermoset resins.

Investigating the total antioxidant capacity of gluten-free grains with miniaturized near-infrared spectrometer

NIR news ◽  
2019 ◽  
Vol 30 (5-6) ◽  
pp. 35-38
Author(s):  
Verena Wiedemair ◽  
Christian Wolfgang Huck
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Near Infrared ◽  
Least Squares Regression ◽  
Test Set ◽  
Total Antioxidant ◽  
Infrared Spectrometer ◽  
Near Infrared Spectrometer ◽  
Infrared Instruments

The use of ever smaller near-infrared instruments is becoming more and more prevalent, since they are cheaper, more versatile and often advertised as high-performance spectrometer. The last claim is rarely verified by independent researchers, which is why the presented work evaluates the performance of three hand-held spectrometers in comparison to a benchtop instrument. Seventy-seven samples comprising buckwheat, millet and oat were investigated for their total antioxidant capacity using Folin–Ciocalteu and near-infrared spectroscopy. Partial least squares regression models were established using cross- and test set validation. Results showed that all instruments were able to predict total antioxidant capacity to some extent. The coefficients of determinations ranged from 0.823 to 0.951 for cross-validated and from 0.849 to 0.952 for test set validated models. Errors for cross-validated models ranged from 1.11 to 2.08 mgGAE/g and for test set validated models from 1.02 to 1.86 mgGAE/g.

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