scholarly journals Changes in Inflammatory Markers after Administration of Tocilizumab in COVID-19: A Single-Center Retrospective Study

2021 ◽  
Vol 11 (1) ◽  
pp. 107
Author(s):  
Anna Olewicz-Gawlik ◽  
Barbara Ginter-Matuszewska ◽  
Mikołaj Kamiński ◽  
Agnieszka Adamek ◽  
Maciej Bura ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Lactate Dehydrogenase ◽  
Prognostic Factor ◽  
Confidence Interval ◽  
Serum Concentration ◽  
Inflammatory Markers ◽  
Serum Levels ◽  
Serum Concentrations ◽  
Single Center ◽  
Area Under Roc Curve

The COVID-19 pandemic requires the development of effective methods for the treatment of severe cases. We aimed to describe clinical outcomes and changes in inflammatory markers in Polish patients treated with tocilizumab. The medical charts of SARS-CoV-2-positive patients treated in the Department of Infectious Diseases between 4 March and 2 September 2020 were retrospectively analyzed. The patients who received tocilizumab according to the Polish Association of Epidemiologists and Infectiologists guidelines were selected for the study. We identified 29 individuals who received tocilizumab, out of whom 11 (37.9%) died. The individuals who died had significantly higher maximal interleukin-6 (IL-6) and lactate dehydrogenase (LDH) serum levels than survivors. After administration of tocilizumab, further increase in LDH and IL-6 was a prognostic factor for unfavorable outcomes. Among inflammatory markers, 7-day mean of IL-6 serum concentration was the best predictor of death (cut-off: ≥ 417 pg/mL; area under ROC curve = 0.81 [95% Confidence Interval: 0.63–0.98]). The serum concentrations of inflammatory markers before administration of tocilizumab did not predict the outcome, whereas IL-6 and LDH measurements after administration of tocilizumab seemed to be of predictive value.

scholarly journals THU0277 THE EXPRESSION OF IFNΑ, INFΒ AND INFΓ AND SERUM LEVELS OF THOSE CYTOKINES IN SJÖGREN’S SYNDROME PATIENTS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 365.1-365
Author(s):  
M. Maślińska ◽  
A. Paradowska-Gorycka ◽  
A. Wajda ◽  
K. Kostyra-Grabczak ◽  
B. Kwiatkowska
Keyword(s):  
Serum Concentration ◽  
Rheumatoid Factor ◽  
Mrna Level ◽  
Serum Levels ◽  
Type I ◽  
Type I Ifn ◽  
Serum Concentrations ◽  
Expression Levels ◽  
Schirmer’S Test ◽  
Non Parametric

Background:In the pathogensis of autoimmune mediated diseases, such as Sjögren’s syndrome (SS), interferons (IFN) and IFN pathway activation play a vital role.Objectives:We planned to assess IFNα, INFβ and INFγ expression and IFNs serum levels in SS patients and correlation of these parameters with: autoantibodies specific for SS, serum concentration of C3, C4 component of complement (C3, C4), rheumatoid factor (RF), gammaglobulins, focus score (FS) and eye dryness symptoms.Methods:Whole blood RNA was isolated from 77 SS patients [F91%vsM9%]; mean age 49,69±15.36; SS diagnosis according to EULAR/ACR 2016 criteria. The analysis of INFα, - β and - γ expression levels was based on validated TaqMan probes by ΔCT methods. Serum concentrations of rheumatoid factor (RF), C3- and C4 complement components (mg/dL) and gammaglobulins (g/dL), were assessed. Anti-Ro/SSA and/or anti-La/SSB autoantibodies were assessed by semiquantitative immunoblotting evaluation. The eye dryness and keratoconjunctivitis sicca were confirmed with Schirmer’s test (score of less than 5 mm/5’) and the ocular staining score (OSS) using lissamine green and fluorescein staining. The biopsy of minor salivary gland was performed with the histopathological evaluation of FS. The study was approved by the Bioethics Committee. Differences between groups of patients were determined using non-parametric Mann-Whitney U test or Kruskall-Wallis test with Dunn’s post hoc. Correlations were determined using non-parametric Spearman test. The level of statistical significance was set at p < 0.05.Results:IFNβ had the highest expression levels among IFNs and IFNβ serum concentrations were higher than those of IFNα and -γ. In cases with high IFNβ serum concentration lower IFNβ expression was observed. There was a highly significant correlation between IFNα and IFNβ expression (r =0.6;p=0.001). IFNβ expression (p=0.059) was higher in the group of younger (<45 y.o.) patients (n= 23; 29.9%) as compared to the group of older individuals (at least 45 y.o.). In patients with SS-A / Ro antibodies with strong antigen binding affinity (3) IFN β expression and IFNβ serum levels were highest of all IFNs. The presence of anti La/SS-B antibodies was associated with the increased IFNβ expression while not with the increased IFNβ serum concentration. In terms of IFNα expression and protein level, RF(+)patients had average higher values compared to RF(-) patients. The average mRNA level of IFNα was about 3 times lower in patients with low C3 serum concentration compared to patients with normal C3 serum concentration values. IFNβ mRNA level was 2.5 times lower in patients with low Schirmer’s test (<5mm/5’) in comparison to patients with Schirmer’s test>5mm/5’; Schirmer’s test <5mm/5’ was associated with higher IFNβ serum concentration.Conclusion:Type I IFN signature predominates in the peripheral blood of studied patients. Presented results confirmed the pivotal role of type I IFN in the disease process. The serum concentration of IFNβ and the expression of IFNβ were the highest values of those parameters for cytokines assessed in this study. A positive correlation between IFNα and IFNβ mRNA levels has been observed.Disclosure of Interests:None declared

scholarly journals Incidence of postoperative, major, adverse cardiac events in patients undergoing carotid endarterectomy: A single-center, retrospective study

2022 ◽  
Vol 10 ◽  
pp. 205031212110703
Author(s):  
Aphichat Suphathamwit ◽  
Chutima Leewatchararoongjaroen ◽  
Pongprueth Rujirachun ◽  
Kittipatr Poopong ◽  
Apichaya Leesakul ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Heart Failure ◽  
Congestive Heart Failure ◽  
Retrospective Study ◽  
Confidence Interval ◽  
Carotid Endarterectomy ◽  
Major Adverse Cardiac Events ◽  
Single Center ◽  
Cardiac Events ◽  
Adverse Cardiac Events

Objective: This study aimed to determine the incidence of postoperative major adverse cardiac events for patients undergoing carotid endarterectomy. Methods: This single-center, retrospective study recruited 171 carotid endarterectomy patients between January 1999 and June 2018. Patients who received a carotid endarterectomy in conjunction with other surgery were excluded. The primary outcomes were the incidences of major adverse cardiac events (comprising myocardial infarction, significant arrhythmias, congestive heart failure, and cardiac death) within 7 days, 7–30 days, and > 30 days–1 year, postoperatively. The secondary outcomes were the factors related to major adverse cardiac events and the incidence of postoperative stroke. The patients’ charts were reviewed, and direct contact was made with them to obtain information on their status post discharge. Results: The incidences of major adverse cardiac events within 7 days, 7–30 days, and >30 days–1 year of the carotid endarterectomy were 3.5% of patients (95% confidence interval: 0.008–0.063), 1.2% (95% confidence interval: 0.004–0.028), and 1.8% (95% confidence interval: 0.002–0.037), respectively. The major adverse cardiac events occurring within 7 days were arrhythmia (2.3% of patients), cardiac arrest (1.8%), myocardial infarction (1.2%), and congestive heart failure (1.2%), while the corresponding postoperative stroke rate was 4.7%. Conclusion: The 7-day incidence of major adverse cardiac events after the carotid endarterectomy was 3.5%. The most common major adverse cardiac event during that period was cardiac arrhythmia.

scholarly journals TNF-α serum levels are elevated in women with clinically symptomatic uterine fibroids

2018 ◽  
Vol 32 ◽  
pp. 205873841877946 ◽  
Author(s):  
Michał Ciebiera ◽  
Marta Włodarczyk ◽  
Małgorzata Wrzosek ◽  
Cezary Wojtyła ◽  
Błażej Męczekalski ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Female Genital Tract ◽  
Uterine Fibroids ◽  
Serum Levels ◽  
Control Group ◽  
Study Group ◽  
Phase Reaction ◽  
Serum Concentrations ◽  
Tnf Α ◽  
The Impact

Uterine fibroids (UFs) are one of the most common pathologies of the female genital tract. The incidence of UFs has been estimated at 25–80%. Tumor necrosis factor (TNF)-α is a cell-signaling protein involved in systemic inflammation and is one of the cytokines responsible for the acute phase reaction. The aim of the study was to evaluate the impact of clinically symptomatic UFs on TNF-α serum levels. A total of 149 Caucasian women were included: 85 patients admitted for surgery due to clinically symptomatic UFs (n = 85; study group) and 64 age-matched UF-free controls (n = 64). TNF-α serum concentrations between the groups were compared. Receiver operating characteristic (ROC) curves were also used as a statistical model to evaluate TNF-α as a marker for UFs. Mean TNF-α serum concentration in the study group was 0.34 ± 0.14 pg/mL; (in half of the subjects, the level did not exceed 0.39 pg/mL. Mean TNF-α serum concentration in the control group was 0.17 ± 0.09 pg/mL; in half of the subjects, the level did not exceed 0.14 pg/mL. The difference was statistically significant. Using the area under the ROC curve, we found that TNF-α serum concentration of 0.34 pg/mL can be used as a predictor for UFs in selected populations. In our study, we confirmed higher TNF-α serum concentrations in women with clinically symptomatic UFs.

Elevated CXCL10 Serum Levels in Measles Virus Primary Infection and Reinfection Correlate With the Serological Stage and Hospitalization Status

2020 ◽  
Vol 222 (12) ◽  
pp. 2030-2034
Author(s):  
Georg Semmler ◽  
Hannah Griebler ◽  
Stephan W Aberle ◽  
Karin Stiasny ◽  
Lukas Richter ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Primary Infection ◽  
Measles Virus ◽  
Viral Infections ◽  
Area Under The Curve ◽  
Serum Levels ◽  
Hospitalized Patients ◽  
Serum Concentrations ◽  
Vaccine Failure ◽  
Potential Marker

Abstract We quantified serum concentrations of chemokine CXCL10 in 288 patients with measles virus (MeV) primary infection and 16 patients with reinfection (vaccine failure). CXCL10 peaked with emergence of IgM antibodies and was elevated in hospitalized patients (3233 vs 1930 pg/mL, P &lt; .0001). CXCL10 differed between primary and reinfection (1958 vs 932 pg/mL, P = .0402). In comparison to other viral infections with rash-like symptoms, CXCL10 was highly elevated in MeV infection (area under the curve = 0.935; 95% confidence interval, .905–.965; P &lt; .0001). CXCL10 is a potential marker for diagnosis, stage, and severity of MeV infection.

scholarly journals Systemic inflammatory markers and psychophysical olfactory scores in coronavirus disease 2019 patients: is there any correlation?

2021 ◽  
pp. 1-6
Author(s):  
L A Vaira ◽  
A De Vito ◽  
G Deiana ◽  
C Pes ◽  
F Giovanditto ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Clinical Research ◽  
Inflammatory Markers ◽  
Serum Levels ◽  
Neutrophil To Lymphocyte Ratio ◽  
C Reactive Protein ◽  
Lymphocyte Ratio ◽  
Reactive Protein ◽  
Clinical Research Center ◽  
D Dimer

Abstract Objective To analyse the correlations between olfactory psychophysical scores and the serum levels of D-dimer, C-reactive protein, ferritin, lactate dehydrogenase, procalcitonin and neutrophil-to-lymphocyte ratio in coronavirus disease 2019 patients. Methods Patients underwent psychophysical olfactory assessment with the Connecticut Chemosensory Clinical Research Center test, and determination of blood serum levels of the inflammatory markers D-dimer, C-reactive protein, ferritin, lactate dehydrogenase, procalcitonin and neutrophil-to-lymphocyte ratio within 10 days of the clinical onset of coronavirus disease 2019 and 60 days after. Results Seventy-seven patients were included in this study. D-dimer, procalcitonin, ferritin and neutrophil-to-lymphocyte ratio correlated significantly with severe coronavirus disease 2019. No significant correlations were found between baseline and 60-day Connecticut Chemosensory Clinical Research Center test scores and the inflammatory markers assessed. Conclusion Olfactory disturbances appear to have little prognostic value in predicting the severity of coronavirus disease 2019 compared to D-dimer, ferritin, procalcitonin and neutrophil-to-lymphocyte ratio. The lack of correlation between the severity and duration of olfactory disturbances and serum levels of inflammatory markers seems to further suggest that the pathogenetic mechanisms underlying the loss of smell in coronavirus disease 2019 patients are related to local rather than systemic inflammatory factors.

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