Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability

DNA nanostructures, owing to their controllable and adaptable nature, have been considered as highly attractive nanoplatforms for biomedical applications in recent years. However, their use in the biological environment has been restricted by low cellular transfection efficiency in mammalian cells, weak stability under physiological conditions, and endonuclease degradation. Herein, we demonstrate an effective approach to facilitate fast transfection of DNA nanostructures and enhance their stability by encapsulating DNA origami with a biocompatible cationic protein (cHSA) via electrostatic interaction. The coated DNA origami is found to be stable under physiological conditions. Moreover, the cHSA coating could significantly improve the cellular transfection efficiency of DNA origami, which is essential for biological applications.

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How SIMS microscopy can be used in medicine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100132649 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1602-1603

Author(s):

Philippe Fragu

Keyword(s):

Mass Spectrometry ◽

Biomedical Applications ◽

Secondary Ion Mass Spectrometry ◽

Chemical Elements ◽

Biological Applications ◽

The Past ◽

Potential Source ◽

Secondary Ion ◽

Chemical Integrity ◽

Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

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Secreted CXCL12 (SDF-1) forms dimers under physiological conditions

Biochemical Journal ◽

10.1042/bj20111341 ◽

2012 ◽

Vol 442 (2) ◽

pp. 433-442 ◽

Cited By ~ 35

Author(s):

Paramita Ray ◽

Sarah A. Lewin ◽

Laura Anne Mihalko ◽

Sasha-Cai Lesher-Perez ◽

Shuichi Takayama ◽

...

Keyword(s):

Breast Cancer ◽

Extracellular Space ◽

Mammalian Cells ◽

Cell Signalling ◽

Xenograft Model ◽

Physiological Conditions ◽

Cxc Chemokine ◽

Chemokine Ligand ◽

Chemokine Cxcl12 ◽

And Function

Chemokine CXCL12 (CXC chemokine ligand 12) signalling through CXCR (CXC chemokine receptor) 4 and CXCR7 has essential functions in development and underlies diseases including cancer, atherosclerosis and autoimmunity. Chemokines may form homodimers that regulate receptor binding and signalling, but previous studies with synthetic CXCL12 have produced conflicting evidence for homodimerization. We used bioluminescence imaging with GL (Gaussia luciferase) fusions to investigate dimerization of CXCL12 secreted from mammalian cells. Using column chromatography and GL complementation, we established that CXCL12 was secreted from mammalian cells as both monomers and dimers. Secreted CXCL12 also formed homodimers in the extracellular space. Monomeric CXCL12 preferentially activated CXCR4 signalling through Gαi and Akt, whereas dimeric CXCL12 more effectively promoted recruitment of β-arrestin 2 to CXCR4 and chemotaxis of CXCR4-expressing breast cancer cells. We also showed that CXCR7 preferentially sequestered monomeric CXCL12 from the extracellular space and had minimal effects on dimeric CXCL12 in cell-based assays and an orthotopic tumour xenograft model of human breast cancer. These studies establish that CXCL12 secreted from mammalian cells forms homodimers under physiological conditions. Since monomeric and dimeric CXCL12 have distinct effects on cell signalling and function, our results have important implications for ongoing efforts to target CXCL12 pathways for therapy.

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Oligonucleotides carrying nucleoside antimetabolites as potential prodrugs

Current Medicinal Chemistry ◽

10.2174/0929867328666211129124039 ◽

2021 ◽

Vol 28 ◽

Author(s):

Carme Fàbrega ◽

Anna Clua ◽

Ramon Eritja ◽

Anna Aviñó

Keyword(s):

Antisense Oligonucleotides ◽

Biomedical Applications ◽

Enzymatic Degradation ◽

Research Effort ◽

Synergetic Effect ◽

Chemotherapeutic Agents ◽

Dna Nanostructures ◽

Chemically Modified ◽

Near Future ◽

Large Research

Background: Nucleoside and nucleobase antimetabolites are an important class of chemotherapeutic agents for the treatment of cancer as well as other diseases. Introduction: In order to avoid undesirable side effects, several prodrug strategies have been developed for that purpose. In the present review, we describe a relatively unknown strategy that consists in the use of oligonucleotides modified with nucleoside antimetabolites as prodrugs. Method: The active nucleotides are generated by enzymatic degradation once incorporated into cells. This strategy has attracted large interest and is very active at present due to the continuous developments made on therapeutic oligonucleotides and the recent advances in the field of nanomaterials and nanomedicine. Results: A large research effort was done mainly in the improvement of the antiproliferative properties of nucleoside homopolymers, but recently, chemically modified aptamers, antisense oligonucleotides and/or siRNA carrying antiproliferative nucleotides have demonstrated a great potential due to the synergetic effect of both therapeutic entities. In addition, DNA nanostructures with interesting properties have been built to combine antimetabolites and enhancers of cellular uptake in the same scaffold. Finally, protein nanoparticles functionalized with receptor-binders and antiproliferative oligomers represent a new avenue for a more effective treatment in cancer therapy. Conclusion: It is expected that oligonucleotides carrying nucleoside antimetabolites will be considered as potential drugs in the near future for biomedical applications.

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Improved dispersibility of nano-graphene oxide by amphiphilic polymer coatings for biomedical applications

RSC Advances ◽

10.1039/c6ra15531f ◽

2016 ◽

Vol 6 (81) ◽

pp. 77818-77829 ◽

Cited By ~ 10

Author(s):

Rana Imani ◽

Wei Shao ◽

Shahriar Hojjati Emami ◽

Shahab Faghihi ◽

Satya Prakash

Keyword(s):

Graphene Oxide ◽

Biomedical Applications ◽

Aqueous Media ◽

Polymer Coatings ◽

Amphiphilic Polymer ◽

Nano Materials ◽

Biological Applications ◽

The Poor ◽

Nano Graphene

The poor dispersibility of graphene-based nano-materials in aqueous media is a crucial limitation in their biological applications.

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Overview of DNA Self-Assembling: Progresses in Biomedical Applications

Pharmaceutics ◽

10.3390/pharmaceutics10040268 ◽

2018 ◽

Vol 10 (4) ◽

pp. 268 ◽

Cited By ~ 6

Author(s):

Andreia Jorge ◽

Ramon Eritja

Keyword(s):

Programming Languages ◽

Biomedical Applications ◽

Great Promise ◽

Dna Nanostructures ◽

Modular Assembly ◽

Molecular Programming ◽

Self Assembling ◽

Theranostic Agents ◽

Assembly Principles

Molecular self-assembling is ubiquitous in nature providing structural and functional machinery for the cells. In recent decades, material science has been inspired by the nature’s assembly principles to create artificially higher-order structures customized with therapeutic and targeting molecules, organic and inorganic fluorescent probes that have opened new perspectives for biomedical applications. Among these novel man-made materials, DNA nanostructures hold great promise for the modular assembly of biocompatible molecules at the nanoscale of multiple shapes and sizes, designed via molecular programming languages. Herein, we summarize the recent advances made in the designing of DNA nanostructures with special emphasis on their application in biomedical research as imaging and diagnostic platforms, drug, gene, and protein vehicles, as well as theranostic agents that are meant to operate in-cell and in-vivo.

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Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites

10.1101/845420 ◽

2019 ◽

Author(s):

Helen L. Miller ◽

Sonia Contera ◽

Adam J.M. Wollman ◽

Adam Hirst ◽

Katherine E. Dunn ◽

...

Keyword(s):

Drug Delivery ◽

Single Molecule ◽

Targeted Drug Delivery ◽

Binding Sites ◽

Dna Origami ◽

Target Cells ◽

Dna Nanostructures ◽

Synthetic Dna ◽

Drug Molecules ◽

Targeted Drug

AbstractIntercalation of drug molecules into synthetic DNA nanostructures formed through self-assembled origami has been postulated as a valuable future method for targeted drug delivery. This is due to the excellent biocompatibility of synthetic DNA nanostructures, and high potential for flexible programmability including facile drug release into or near to target cells. Such favourable properties may enable high initial loading and efficient release for a predictable number of drug molecules per nanostructure carrier, important for efficient delivery of safe and effective drug doses to minimise non-specific release away from target cells. However, basic questions remain as to how intercalation-mediated loading depends on the DNA carrier structure. Here we use the interaction of dyes YOYO-1 and acridine orange with a tightly-packed 2D DNA origami tile as a simple model system to investigate intercalation-mediated loading. We employed multiple biophysical techniques including single-molecule fluorescence microscopy, atomic force microscopy, gel electrophoresis and controllable damage using low temperature plasma on synthetic DNA origami samples. Our results indicate that not all potential DNA binding sites are accessible for dye intercalation, which has implications for future DNA nanostructures designed for targeted drug delivery.

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Stimuli Responsive, Programmable DNA Nanodevices for Biomedical Applications

Frontiers in Chemistry ◽

10.3389/fchem.2021.704234 ◽

2021 ◽

Vol 9 ◽

Author(s):

Udisha Singh ◽

Vinod Morya ◽

Bhaskar Datta ◽

Chinmay Ghoroi ◽

Dhiraj Bhatia

Keyword(s):

Biomedical Applications ◽

Recent Progress ◽

Dna Nanotechnology ◽

Research Area ◽

Biological Applications ◽

Stimuli Responsive ◽

Future Prospects ◽

Dna Structures ◽

Challenges And Opportunities

Of the multiple areas of applications of DNA nanotechnology, stimuli-responsive nanodevices have emerged as an elite branch of research owing to the advantages of molecular programmability of DNA structures and stimuli-responsiveness of motifs and DNA itself. These classes of devices present multiples areas to explore for basic and applied science using dynamic DNA nanotechnology. Herein, we take the stake in the recent progress of this fast-growing sub-area of DNA nanotechnology. We discuss different stimuli, motifs, scaffolds, and mechanisms of stimuli-responsive behaviours of DNA nanodevices with appropriate examples. Similarly, we present a multitude of biological applications that have been explored using DNA nanodevices, such as biosensing, in vivo pH-mapping, drug delivery, and therapy. We conclude by discussing the challenges and opportunities as well as future prospects of this emerging research area within DNA nanotechnology.

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Synthesis and characterizations of nano-silica for biomedical applications

Vietnam Journal of Catalysis and Adsorption ◽

10.51316/jca.2021.039 ◽

2021 ◽

Vol 10 (2) ◽

pp. 114-118

Author(s):

Huyen Nguyen Thi ◽

Tam Lai Thi Thanh ◽

Yudy Paola Monreno Gonzalez ◽

Thinh Nguyen Ngoc ◽

Mai Nguyen Thi Tuyet ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Biomedical Applications ◽

Cetyltrimethylammonium Bromide ◽

Average Length ◽

Biological Applications ◽

Nano Silica ◽

Facile Synthesis ◽

Structure Morphology ◽

Ft Ir

This paper presents a facile synthesis of nano-silica by hydrothermal treatment assisted by cetyltrimethylammonium bromide (CTAB). The effect of CTAB on the morphology of the material was also investigated. Structure, morphology, and composition of the material were studied byvarious methods such as XRD, SEM, FT-IR, and EDX.The results showed that a sample of nanosilica with amount of 1,0 g CTAB at pH 10-11 reached the most appropriate size, with the average length and width are 231,34±48,98 nm và 113,05±16,45 nm, respectively. In addition, the results indicated that the nanoparticles are completely pure, with many silanol groups on the surface, suitable for applications in bone tissue engineering and other biological applications.

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Utilization of Near IR Absorbing Gold Nanocolloids by Green Synthesis

Materials Science Forum ◽

10.4028/www.scientific.net/msf.915.213 ◽

2018 ◽

Vol 915 ◽

pp. 213-219

Author(s):

Beste Elveren ◽

Ümit Hakan Yildiz ◽

Ahu Arslan Yildiz

Keyword(s):

Drug Delivery ◽

Tissue Engineering ◽

Gold Nanoparticles ◽

Binding Kinetics ◽

Biological Applications ◽

Near Ir ◽

Novel Synthesis ◽

Low Toxicity ◽

Biological Environment ◽

Unique Surface

The rapid developments in nanoscience, and its applications on biomedical areas have a large impact on drug delivery, tissue engineering, sensing, and diagnosis. Gold is widely investigated nanomaterial for the last couple of decades, since it has unique surface properties and very low toxicity to biological environment. In this work, we present a novel synthesis of gold nanoparticles (GNPs) exhibiting both visible and near-IR absorbance without agglomeration. The surface of GNPs were analyzed by routine methods and the binding kinetics were investigated by Surface Plasmon Resonance (SPR) Spectroscopy. The unique optical properties of near-IR asorbing GNP colloids hold promise for biological applications.

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