scholarly journals Sodium Alginate/Gelatine Hydrogels for Direct Bioprinting—The Effect of Composition Selection and Applied Solvents on the Bioink Properties

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2669 ◽  
Author(s):  
Dorota Bociaga ◽  
Mateusz Bartniak ◽  
Jacek Grabarczyk ◽  
Karolina Przybyszewska
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Sodium Alginate ◽  
Medical Devices ◽  
Degradation Rate ◽  
Culture Medium ◽  
Biological Response ◽  
Degradation Process ◽  
Biological Properties ◽  
Proliferation Ability

Hydrogels tested and evaluated in this study were developed for the possibility of their use as the bioinks for 3D direct bioprinting. Procedures for preparation and sterilization of hydrogels and the speed of the bioprinting were developed. Sodium alginate gelatine hydrogels were characterized in terms of printability, mechanical, and biological properties (viability, proliferation ability, biocompatibility). A hydrogel with the best properties was selected to carry out direct bioprinting tests in order to determine the parameters of the bioink, adapted to print with use of the designed and constructed bioprinter and provide the best conditions for cell growth. The obtained results showed the ability to control mechanical properties, biological response, and degradation rate of hydrogels through the use of various solvents. The use of a dedicated culture medium as a solvent for the preparation of a bioink, containing the predicted cell line, increases the proliferation of these cells. Modification of the percentage of individual components of the hydrogel gives the possibility of a controlled degradation process, which, in the case of printing of temporary medical devices, is a very important parameter for the hydrogels’ usage possibility—both in terms of tissue engineering and printing of tissue elements replacement, implants, and organs.

Hybrid nanostructured hydroxyapatite–chitosan composite scaffold: bioinspired fabrication, mechanical properties and biological properties

2015 ◽  
Vol 3 (23) ◽  
pp. 4679-4689 ◽  
Author(s):  
Ya-Ping Guo ◽  
Jun-Jie Guan ◽  
Jun Yang ◽  
Yang Wang ◽  
Chang-Qing Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Biological Properties ◽  
Chitosan Composite

A bioinspired strategy has been developed to fabricate a hybrid nanostructured hydroxyapatite–chitosan composite scaffold for bone tissue engineering.

scholarly journals Additive manufacturing of PLA-based scaffolds intended for bone regeneration and strategies to improve their biological properties

e-Polymers ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 571-599
Author(s):  
Ricardo Donate ◽  
Mario Monzón ◽  
María Elena Alemán-Domínguez
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Additive Manufacturing ◽  
Bone Regeneration ◽  
Polylactic Acid ◽  
State Of The Art ◽  
Biological Properties ◽  
Design Stage ◽  
Bone Scaffolds ◽  
Regeneration Of Bone

AbstractPolylactic acid (PLA) is one of the most commonly used materials in the biomedical sector because of its processability, mechanical properties and biocompatibility. Among the different techniques that are feasible to process this biomaterial, additive manufacturing (AM) has gained attention recently, as it provides the possibility of tuning the design of the structures. This flexibility in the design stage allows the customization of the parts in order to optimize their use in the tissue engineering field. In the recent years, the application of PLA for the manufacture of bone scaffolds has been especially relevant, since numerous studies have proven the potential of this biomaterial for bone regeneration. This review contains a description of the specific requirements in the regeneration of bone and how the state of the art have tried to address them with different strategies to develop PLA-based scaffolds by AM techniques and with improved biofunctionality.

scholarly journals Porous Cellulose-Collagen Scaffolds For Soft Tissue Regeneration: Influence of Cellulose Derivatives On Mechanical Properties And Compatibility With Adipose-Derived Stem Cells.

2022 ◽  
Author(s):  
Katarína Kacvinská ◽  
Martina Trávničková ◽  
Lucy Vojtová ◽  
Petr Poláček ◽  
Jana Dorazilová ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Adhesion ◽  
Soft Tissue ◽  
Vascular Tissue ◽  
Biological Properties ◽  
Cellulose Derivatives ◽  
Adipose Derived Stem Cells ◽  
Soft Tissue Regeneration

Abstract This study deals with cellulose derivatives in relation to the collagen fibrils in composite collagen-cellulose scaffolds for soft tissue engineering. Two types of cellulose, i.e., oxidized cellulose (OC) and carboxymethyl cellulose (CMC), were blended with collagen (Col) to enhance its elasticity, stability and sorptive biological properties, e.g. hemostatic and antibacterial features. The addition of OC supported the resistivity of the Col fibrils in a dry environment, while in a moist environment OC caused a radical drop. The addition of CMC reduced the mechanical strength of the Col fibrils in both environments. The elongation of the Col fibrils was increased by both types of cellulose derivatives in both environments, which is closely related to tissue like behaviour. In these various mechanical environments, the ability of human adipose-derived stem cells (hADSCs) to adhere and proliferate was significantly greater in the Col and Col/OC scaffolds than in the Col/CMC scaffold. This is explained by deficient mechanical support and loss of stiffness due to the high swelling capacity of CMC. Although Col/OC and Col/CMC acted differently in terms of mechanical properties, both materials were observed to be cytocompatible, with varying degrees of further support for cell adhesion and proliferation. While Col/OC can serve as a scaffolding material for vascular tissue engineering and for skin tissue engineering, Col/CMC seems to be more suitable for moist wound healing, e.g. as a mucoadhesive gel for exudate removal, since there was almost no cell adhesion.

scholarly journals Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1930 ◽  
Author(s):  
Ming-You Shie ◽  
Jian-Jr Lee ◽  
Chia-Che Ho ◽  
Ssu-Yin Yen ◽  
Hooi Yee Ng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physical Properties ◽  
Dermal Fibroblast ◽  
Sol Gel ◽  
Biological Properties ◽  
Human Dermal Fibroblast ◽  
Natural Material ◽  
Matrix Remodeling ◽  
Proliferation Markers

Gelatin-methacryloyl (GelMa) is a very versatile biomaterial widely used in various biomedical applications. The addition of methacryloyl makes it possible to have hydrogels with varying mechanical properties due to its photocuring characteristics. In addition, gelatin is obtained and derived from natural material; thus, it retains various cell-friendly motifs, such as arginine-glycine-aspartic acid, which then provides implanted cells with a friendly environment for proliferation and differentiation. In this study, we fabricated human dermal fibroblast cell (hDF)-laden photocurable GelMa hydrogels with varying physical properties (5%, 10%, and 15%) and assessed them for cellular responses and behavior, including cell spreading, proliferation, and the degree of extracellular matrix remodeling. Under similar photocuring conditions, lower concentrations of GelMa hydrogels had lower mechanical properties than higher concentrations. Furthermore, other properties, such as swelling and degradation, were compared in this study. In addition, our findings revealed that there were increased remodeling and proliferation markers in the 5% GelMa group, which had lower mechanical properties. However, it was important to note that cellular viabilities were not affected by the stiffness of the hydrogels. With this result in mind, we attempted to fabricate 5–15% GelMa scaffolds (20 × 20 × 3 mm3) to assess their feasibility for use in skin regeneration applications. The results showed that both 10% and 15% GelMa scaffolds could be fabricated easily at room temperature by adjusting several parameters, such as printing speed and extrusion pressure. However, since the sol-gel temperature of 5% GelMa was noted to be lower than its counterparts, 5% GelMa scaffolds had to be printed at low temperatures. In conclusion, GelMa once again was shown to be an ideal biomaterial for various tissue engineering applications due to its versatile mechanical and biological properties. This study showed the feasibility of GelMa in skin tissue engineering and its potential as an alternative for skin transplants.

scholarly journals TiO2 doped chitosan/hydroxyapatite/halloysite nanotube membranes with enhanced mechanical properties and osteoblast-like cell response for application in bone tissue engineering

RSC Advances ◽  
2019 ◽  
Vol 9 (68) ◽  
pp. 39768-39779
Author(s):  
Sarim Khan ◽  
Viney Kumar ◽  
Partha Roy ◽  
Patit Paban Kundu
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Response ◽  
Halloysite Nanotubes ◽  
Biological Properties ◽  
Halloysite Nanotube ◽  
Nanotube Membranes ◽  
Hydroxyapatite Composite

This two-stage study aims to optimize the amount of halloysite nanotubes and TiO2 in a chitosan/nano-hydroxyapatite composite to tailor the mechanical and biological properties for application in bone tissue engineering.

In Vitro Cytotoxicity Study of the Nano-Hydroxyapatite/Bacterial Cellulose Nanocomposites

2009 ◽  
Vol 610-613 ◽  
pp. 1011-1016 ◽  
Author(s):  
Yan Mei Chen ◽  
Ting Fei Xi ◽  
Yu Dong Zheng ◽  
Yi Zao Wan
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Biological Response ◽  
Biological Properties ◽  
In Vitro Cytotoxicity ◽  
National Standard ◽  
Target Cells

The nanocomposite of nano-hydroxyapatite/bacterial cellulose (nHA/BC) obtained by depositing in simulated body fluid (SBF), incorporating their excellent mechanical and biological properties, is expected to have potential applications in bone tissue engineering. However, the biological response evaluation of biomaterials is required to provide useful information to improve their design and application. In this article, the in vitro cytotoxicity of composites nHA/BC as well as its degradation residues was studied. Scanning electron microscopy (SEM) was used to observe the morphology of original materials and their degradation residues. The degree of degradation was evalued by measuring the concentration of reducing sugar (glucose) by ultraviolet spectrophotometer. Bone-forming osteoblasts (OB) and infinite culture cell line L929 fibroblasts were used to measure the cytotocixity of materials with MTT assay. Both kinds of cells in infusion proliferate greatly in a normal form and their relative growth rate (RGR) exceeds by 75%, which shows the cytotoxicity of materials is graded as 0~1, according to the national standard. Nevertheless, bone-forming OB cells, as a kind of target cells, are more susceptive on the cytotoxicity than infinite culture fibroblast cells L929. The results suggest the nanocomposite of nHA/BC without cytotoxicity is greatly promising as a kind of scaffold materials for bone tissue engineering and tissue functional cells are more suited to evaluate the cytotoxicity of biomedical materials.

scholarly journals Synergistic Effect of Carbon Nanotubes and Graphene on Diopside Scaffolds

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Tingting Liu ◽  
Ping Wu ◽  
Chengde Gao ◽  
Pei Feng ◽  
Tao Xiao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Network Architecture ◽  
Body Fluid ◽  
Degradation Rate ◽  
Synergetic Effect ◽  
Cell Culturing ◽  
3D Network ◽  
The Matrix

A synergetic effect between carbon nanotubes (CNTs) and graphene on diopside (Di) scaffolds was demonstrated. 3D network architecture in the matrix was formed through the 1D CNTs inlaid among the 2D graphene platelets (GNPs). The mechanical properties of the CNTs/GNPs/Di scaffolds were significantly improved compared with the CNTs/Di scaffolds and GNPs/Di scaffolds. In addition, the scaffolds exhibited excellent apatite-forming ability, a modest degradation rate, and stable mechanical properties in simulated body fluid (SBF). Moreover, cell culturing tests indicated that the scaffolds supported the cells attachment and proliferation. Taken together, the CNTs/GNPs/Di scaffolds offered great potential for bone tissue engineering.

scholarly journals Peracetic Acid Sterilization Induces Divergent Biological Response in Polymeric Tissue Engineering Scaffolds

2019 ◽  
Vol 9 (18) ◽  
pp. 3682
Author(s):  
Suyog Yoganarasimha ◽  
Al Best ◽  
Parthasarathy A. Madurantakam
Keyword(s):  
Tissue Engineering ◽  
Cell Survival ◽  
Peracetic Acid ◽  
Degradation Kinetics ◽  
Biological Effects ◽  
Sodium Thiosulfate ◽  
Biological Response ◽  
Biological Properties ◽  
Tissue Engineering Scaffolds ◽  
Polymeric Scaffolds

Synthetic polymers offer control over composition, architecture, mechanical properties and degradation kinetics. Predictable sterilization of synthetic polymeric scaffolds made from low temperature melting polymers, remains a challenge to clinical translation. We previously demonstrated successful room temperature sterilization of electrospun polycaprolactone scaffolds (ePCL) using peracetic acid (PA). The current paper investigates the effects of PA sterilization on two different scaffolds types—ePCL and commercially available porous polystyrene (Alvetex®) scaffolds using mouse calvarial osteoblasts cell line (MC3T3) and Live-Dead Assay. We report cytotoxicity in PA-treated ePCL scaffolds (PA-ePCL), while control scaffolds strongly supported cell survival. Treatment of PA-ePCL scaffolds with known methods of PA residual elimination (sodium thiosulfate, catalase, washing and aeration) had minimal effect on MC3T3 survival. However, incubation in 80% ethanol for 30 min successfully eliminated the toxic PA residuals and restored scaffold cytocompatibility. On the other hand, PA treatment of Alvetex® scaffolds induced diametrically opposite effects: cell survival and proliferation was enhanced after PA exposure and these responses were reversed following ethanol wash. These results suggest that PA treatment can induce different biological effects based on polymer chemistry and scaffold architecture and presents interesting opportunities to modulate biological properties of tissue engineering scaffolds.

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