scholarly journals Bacterial Biofilm Formation Using PCL/Curcumin Electrospun Fibers and Its Potential Use for Biotechnological Applications

Materials ◽  
2020 ◽  
Vol 13 (23) ◽  
pp. 5556
Author(s):  
Daniella Alejandra Pompa-Monroy ◽  
Paulina Guadalupe Figueroa-Marchant ◽  
Syed G. Dastager ◽  
Meghana Namdeo Thorat ◽  
Ana Leticia Iglesias ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Food Industry ◽  
Electrospun Nanofibers ◽  
Bacterial Biofilm ◽  
Bacterial Biofilms ◽  
Scanning Calorimetry ◽  
Metabolites Production ◽  
Pathogenic Biofilms ◽  
Tga And Dsc ◽  
Potential Use

Electrospun nanofibers are used for many applications due to their large surface area, mechanical properties, and bioactivity. Bacterial biofilms are the cause of numerous problems in biomedical devices and in the food industry. On the other hand, these bacterial biofilms can produce interesting metabolites. Hence, the objective of this study is to evaluate the efficiency of poly (Ɛ- caprolactone)/Curcumin (PCL/CUR) nanofibers to promote bacterial biofilm formation. These scaffolds were characterized by scanning electron microscopy (SEM), which showed homogeneous fibers with diameters between 441–557 nm; thermogravimetric analysis and differential scanning calorimetry (TGA and DSC) demonstrated high temperature resilience with degradation temperatures over >350 °C; FTIR and 1H-NMR serve as evidence of CUR incorporation in the PCL fibers. PCL/CUR scaffolds successfully promoted the formation of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa biofilms. These results will be valuable in the study of controlled harvesting of pathogenic biofilms as well as in metabolites production for biotechnological purposes.

Plasma-treated water affects Listeria monocytogenes vitality and biofilm formation

2020 ◽  
Author(s):  
Oliver Handorf ◽  
Viktoria Isabella Pauker ◽  
Thomas Weihe ◽  
Uta Schnabel ◽  
Eric Freund ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Food Industry ◽  
Microwave Plasma ◽  
Industrial Applications ◽  
Bacterial Biofilm ◽  
Treated Water ◽  
Force Microscopy ◽  
Cell Vitality ◽  
Pretreatment Time

Abstract Plasma-generated compounds (PGCs) such as plasma-processed air (PPA) or plasma-treated water (PTW) offer an increasingly important alternative for the treatment of microorganisms in hard-to-reach areas found in several industrial applications including the food industry. To this end, we studied the antimicrobial capacity of plasma-treated water on the vitality and biofilm formation of Listeria monocytogenes, a common food spoilage microorganism. Using a microwave plasma (MidiPLexc), 10 ml of deionized water was treated for 100 s, 300 s and 900 s (pretreatment time) and the bacterial biofilm was subsequently exposed to the PTW for 1 min, 3 min and 5 min (posttreatment time) for each pretreatment time separately. Colony-forming units (CFU), metabolic activity, and cell vitality were reduced for 4.7 log10, 47.9%, and 69.5%, respectively. Live/dead staining and fluorescence microscopy showed a positive correlation between treatment and incubation times and reduction in vitality. Atomic force microscopy indicated a change in the plasticity of the bacteria. These results suggest a promising antimicrobial impact of plasma-treated water on Listeria monocytogenes, which may lead to more targeted applications of plasma decontamination in the food industry in the future.

scholarly journals Antibacterial and antibiofilm effects of garlic (Allium sativum), ginger (Zingiber officinale) and mint (Mentha piperta) on Escherichia coli biofilms

2021 ◽  
Vol 4 (2) ◽  
pp. 166
Author(s):  
Ndaindila Haindongo ◽  
Amara Anyogu ◽  
Osmond Ekwebelem ◽  
Christian Anumudu ◽  
Helen Onyeaka
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Food Industry ◽  
Allium Sativum ◽  
Zingiber Officinale ◽  
Bacterial Survival ◽  
Planktonic Cells ◽  
E Coli ◽  
Foodborne Outbreaks ◽  
Potential Use

Biofilms are a significant concern in the food industry because of their potential to enhance bacterial survival and cause foodborne outbreaks. Escherichia coli (E. coli) is among the leading pathogens responsible for foodborne outbreaks and this can be attributed to its ability to form biofilms in food containers and food preparatory surfaces. The purpose of this study was to investigate the antibacterial and antibiofilm properties of garlic, ginger and mint and their potential to inhibit E.coli and biofilm formation. Disc diffusion assays and 96-well plate crystal violet-based methods were used to achieve these objectives. The plant extracts were diluted from 1 mg/ml to 0.1 mg/ml and incubated 25°C and 37°C to investigate the antimicrobial and antibiofilm effects on E. coli. The findings of this study showed that low temperatures induced the formation of E. coli biofilms and all tested extracts contain a broad spectrum of antibacterial and antibiofilm properties. This study provided new insights on the combined antimicrobial and antibiofilm properties of garlic, ginger and mint against planktonic cells and biofilms of E. coli MG 1655 and highlight the potential use of these extracts in the food industry to prevent biofilm formation by E. coli. 

scholarly journals Inactivation of Efflux Pumps Abolishes Bacterial Biofilm Formation

2008 ◽  
Vol 74 (23) ◽  
pp. 7376-7382 ◽  
Author(s):  
Malin Kvist ◽  
Viktoria Hancock ◽  
Per Klemm
Keyword(s):  
Health Care ◽  
Biofilm Formation ◽  
Efflux Pump ◽  
Efflux Pumps ◽  
Bacterial Biofilm ◽  
Bacterial Biofilms ◽  
Toxic Substances ◽  
Highly Active ◽  
Efflux Pump Inhibitors ◽  
New Treatments

ABSTRACT Bacterial biofilms cause numerous problems in health care and industry; notably, biofilms are associated with a large number of infections. Biofilm-dwelling bacteria are particularly resistant to antibiotics, making it hard to eradicate biofilm-associated infections. Bacteria rely on efflux pumps to get rid of toxic substances. We discovered that efflux pumps are highly active in bacterial biofilms, thus making efflux pumps attractive targets for antibiofilm measures. A number of efflux pump inhibitors (EPIs) are known. EPIs were shown to reduce biofilm formation, and in combination they could abolish biofilm formation completely. Also, EPIs were able to block the antibiotic tolerance of biofilms. The results of this feasibility study might pave the way for new treatments for biofilm-related infections and may be exploited for prevention of biofilms in general.

scholarly journals A Novel Autoantibody Induced by Bacterial Biofilm Conserved Components Aggravates Lupus Nephritis

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenyan Fu ◽  
Yu Liu ◽  
Fangjie Liu ◽  
Chenghua Liu ◽  
Jingjing Li ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Biofilm Formation ◽  
Protein Disulfide Isomerase ◽  
Bacterial Biofilm ◽  
Systemic Autoimmune Disease ◽  
Bacterial Biofilms ◽  
Autoantibody Production ◽  
Disulfide Isomerase ◽  
Protein Disulfide

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease with multiple autoantibody production and often affects the kidneys, known as lupus nephritis. However, the mechanism underlying lupus nephritis development is unclear. Biofilms that protect bacteria from stress are ubiquitous in almost every environment. Here, we identified that a conserved peptide (HU1) derived from DNABII proteins, one of major bacterial biofilm components, was specifically recognized by sera from about 47% patients with SLE. Moreover, the serum anti-HU1 levels showed a significant positive correlation with lupus nephritis occurrence. Presence of antibodies against HU1 in pristane-induced mice aggravated lupus nephritis, although these antibodies also attenuated bacterial biofilm formation. We further identified that antibodies against HU1 cross-recognized protein disulfide isomerase (P4HB) located on the renal cell surface and inhibited the activities of this enzyme. Our findings reveal a novel mechanism underlying the development of lupus nephritis triggered by bacterial biofilms.

scholarly journals Rhamnolipids and surfactin inhibit the growth or formation of oral bacterial biofilm

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ryota Yamasaki ◽  
Aki Kawano ◽  
Yoshie Yoshioka ◽  
Wataru Ariyoshi
Keyword(s):  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Oral Bacteria ◽  
Bacterial Biofilm ◽  
Industrial Processes ◽  
Oral Drug ◽  
Bacterial Biofilms ◽  
Effective Inhibition ◽  
Medical Instruments ◽  
Scanning Electron

Abstract Background Bacteria survive in various environments by forming biofilms. Bacterial biofilms often cause significant problems to medical instruments and industrial processes. Techniques to inhibit biofilm formation are essential and have wide applications. In this study, we evaluated the ability of two types of biosurfactants (rhamnolipids and surfactin) to inhibit growth and biofilm formation ability of oral pathogenic bacteria such as Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Streptococcus sanguinis. Results Rhamnolipids inhibited the growth and biofilm formation ability of all examined oral bacteria. Surfactin showed effective inhibition against S. sanguinis ATCC10556, but lower effects toward A. actinomycetemcomitans Y4 and S. mutans UA159. To corroborate these results, biofilms were observed by scanning electron microscopy (SEM) and confocal microscopy. The observations were largely in concordance with the biofilm assay results. We also attempted to determine the step in the biofilm formation process that was inhibited by biosurfactants. The results clearly demonstrated that rhamnolipids inhibit biofilm formation after the initiation process, however, they do not affect attachment or maturation. Conclusions Rhamnolipids inhibit oral bacterial growth and biofilm formation by A. actinomycetemcomitans Y4, and may serve as novel oral drug against localized invasive periodontitis.

From Input to Output: The Lap/c-di-GMP Biofilm Regulatory Circuit

2020 ◽  
Vol 74 (1) ◽  
pp. 607-631 ◽  
Author(s):  
Alan J. Collins ◽  
T. Jarrod Smith ◽  
Holger Sondermann ◽  
George A. O'Toole
Keyword(s):  
Biofilm Formation ◽  
Secretion System ◽  
Bacterial Biofilm ◽  
Classical Type ◽  
Bacterial Biofilms ◽  
Type I ◽  
Metabolizing Enzymes ◽  
Regulatory Circuit ◽  
The Subject ◽  
And Function

Biofilms are the dominant bacterial lifestyle. The regulation of the formation and dispersal of bacterial biofilms has been the subject of study in many organisms. Over the last two decades, the mechanisms of Pseudomonas fluorescens biofilm formation and regulation have emerged as among the best understood of any bacterial biofilm system. Biofilm formation by P. fluorescens occurs through the localization of an adhesin, LapA, to the outer membrane via a variant of the classical type I secretion system. The decision between biofilm formation and dispersal is mediated by LapD, a c-di-GMP receptor, and LapG, a periplasmic protease, which together control whether LapA is retained or released from the cell surface. LapA localization is also controlled by a complex network of c-di-GMP-metabolizing enzymes. This review describes the current understanding of LapA-mediated biofilm formation by P. fluorescens and discusses several emerging models for the regulation and function of this adhesin.

scholarly journals Lucilia sericata Chymotrypsin Disrupts Protein Adhesin-Mediated Staphylococcal Biofilm Formation

2012 ◽  
Vol 79 (4) ◽  
pp. 1393-1395 ◽  
Author(s):  
Llinos G. Harris ◽  
Yamni Nigam ◽  
James Sawyer ◽  
Dietrich Mack ◽  
David I. Pritchard
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Lucilia Sericata ◽  
Bacterial Biofilm ◽  
Bacterial Biofilms ◽  
Formation Mechanisms ◽  
Chronic Infections ◽  
Content Type ◽  
Effective Activity

ABSTRACTStaphylococcus aureusandStaphylococcus epidermidisbiofilms cause chronic infections due to their ability to form biofilms. The excretions/secretions ofLucilia sericatalarvae (maggots) have effective activity for debridement and disruption of bacterial biofilms. In this paper, we demonstrate how chymotrypsin derived from maggot excretions/secretions disrupts protein-dependent bacterial biofilm formation mechanisms.

scholarly journals Inkjet based 3D Printing of bespoke medical devices that resist bacterial biofilm formation

2020 ◽  
Author(s):  
Yinfeng He ◽  
Belen Begines ◽  
Jeni Luckett ◽  
Jean-Frédéric Dubern ◽  
Andrew L. Hook ◽  
...  
Keyword(s):  
3D Printing ◽  
Cell Fate ◽  
Biofilm Formation ◽  
Bacterial Attachment ◽  
Bacterial Biofilm ◽  
Bacterial Biofilms ◽  
Printing Inks ◽  
A Cell ◽  
3D Printed

AbstractWe demonstrate the formulation of advanced functional 3D printing inks that prevent the formation of bacterial biofilms in vivo. Starting from polymer libraries, we show that a biofilm resistant object can be 3D printed with the potential for shape and cell instructive function to be selected independently. When tested in vivo, the candidate materials not only resisted bacterial attachment but drove the recruitment of host defences in order to clear infection. To exemplify our approach, we manufacture a finger prosthetic and demonstrate that it resists biofilm formation – a cell instructive function that can prevent the development of infection during surgical implantation. More widely, cell instructive behaviours can be ‘dialled up’ from available libraries and may include in the future such diverse functions as the modulation of immune response and the direction of stem cell fate.

scholarly journals Bacterial Biofilms and Their Implications in Pathogenesis and Food Safety

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2117
Author(s):  
Xingjian Bai ◽  
Cindy H. Nakatsu ◽  
Arun K. Bhunia
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
Food Processing ◽  
Biofilm Formation ◽  
Molecular Level ◽  
Bacterial Biofilm ◽  
Bacterial Biofilms ◽  
Microbial Life ◽  
Physiological Processes ◽  
Food Contact Surfaces

Biofilm formation is an integral part of the microbial life cycle in nature. In food processing environments, bacterial transmissions occur primarily through raw or undercooked foods and by cross-contamination during unsanitary food preparation practices. Foodborne pathogens form biofilms as a survival strategy in various unfavorable environments, which also become a frequent source of recurrent contamination and outbreaks of foodborne illness. Instead of focusing on bacterial biofilm formation and their pathogenicity individually, this review discusses on a molecular level how these two physiological processes are connected in several common foodborne pathogens such as Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Escherichia coli. In addition, biofilm formation by Pseudomonas aeruginosa is discussed because it aids the persistence of many foodborne pathogens forming polymicrobial biofilms on food contact surfaces, thus significantly elevating food safety and public health concerns. Furthermore, in-depth analyses of several bacterial molecules with dual functions in biofilm formation and pathogenicity are highlighted.

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