scholarly journals Autologous Platelet Gel (APG): A Preliminary Evaluation of the Mechanical Properties after Activation with Autologous Thrombin and Calcium Chloride

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3941
Author(s):  
Antonio Scarano ◽  
Calogero Bugea ◽  
Lucia Leo ◽  
Pablo Santos de Oliveira ◽  
Felice Lorusso
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Calcium Chloride ◽  
Preliminary Evaluation ◽  
Loading Test ◽  
Platelet Gel ◽  
Group I ◽  
Group Ii ◽  
Autologous Platelet

The tensional and mechanical behavior of regenerative components, grafts, and blood clots represent an essential condition for the success of bone regeneration protocols. Autologous platelet growth factors represent a useful protocol to enhance the soft and hard tissue healing in several fields of medicine and craniofacial surgery. Different protocols for blood concentrates with and without activation have been proposed in literature. The aim of the present study was to investigate in vitro the mechanical properties of autologous platelet gel (APG) with autologous thrombin and calcium chloride. Materials and Methods: A total of 20 APG samples were evaluated; 10 samples were activated by autologous thrombin and calcium chloride (Group I) and 10 samples were non-activated (Group II). The tensile strength and modulus of elasticity were calculated through a static loading test (Lloyd 30 K, Lloyd Instruments Ltd., Segensworth, UK). Results: Group I (activated) reported a tensile strength of 373.5 ± 14.3 MPa, while Group II showed a significantly lower value of of 360.5 ± 16.3 MPa (p < 0.05). The Young’s modulus was 145.3 ± 10.4 MPa for Group I and 140.3 ± 15.3 MPa for Group II (p < 0.05). Conclusions: The effectiveness of the present in vitro simulation showed that the APG activation protocol is able to increase the mechanical characteristics of the blood derivates and could be clinically useful to enhance regenerative procedures.

scholarly journals The Effect of Immersion Time in Three Kinds of Carbonated Beverages on Orthodontic Elastic Latex’s Tensile Strength (In Vitro)

2017 ◽  
Vol 1 ◽  
Author(s):  
Christandi Prana Yuwana ◽  
Christnawati Christnawati ◽  
Cenderawasih Farmasyanti
Keyword(s):  
Tensile Strength ◽  
Immersion Time ◽  
Artificial Saliva ◽  
Treatment Groups ◽  
Carbonated Beverages ◽  
Group I ◽  
Carbonated Beverage ◽  
Group Ii ◽  
Post Hoc

<p class="AbstractContent"><strong>Objective</strong>: To study the effects of immersion time in three kinds of carbonated beverages on orthodontic elastic latex’s tensile strength</p><p class="AbstractContent"><strong>Methods</strong>: Subjects consist of 32 specimens orthodontic elastic latex ¼" and 4.5 oz of power, divided into 8 groups (IA, IB, IC, ID, IIA, IIB, IIC, and IID). Each group are immersed in artificial saliva (pH7) and stored in incubator (37 °C) for 24hrs for group I and 48hrs for group II. Each treatment groups will be immersed in three different carbonated beverages for 90s per day. Elastics being measured its tensile strength for group I at 24th hour, and 48th hour for group II using tension gauge. The data was tested with two way anova and post hoc Tukey.</p><p class="AbstractContent"><strong>Results:</strong> The result shows immersion time decreases elastic latex’s tensile strength (<em>p</em> &lt; 0.05), and carbonated beverages affect elastic latex’s tensile strength (<em>p</em> &lt; 0.05).</p><p class="AbstractContent"><strong>Conclusion:</strong> (1) There is a difference of orthodontic elastic latex tensile strength in the immersion in carbonated beverages for 24 and 48 hours, (2) there is a difference of elastic latex tensile strength orthodontic in the immersion in three kinds of carbonated beverages, and (3) there is interaction between immersion time in carbonated beverages and carbonated beverage brands</p>

Effects of Rest and Exercise on Cardiac Blood Volume Determinations

1997 ◽  
Vol 36 (08) ◽  
pp. 259-264
Author(s):  
N. Topuzović
Keyword(s):  
Radionuclide Ventriculography ◽  
Left Ventricular ◽  
Peak Exercise ◽  
Volume Determination ◽  
Group I ◽  
Lv Volumes ◽  
Group Ii ◽  
Lv Volume

Summary Aim: The purpose of this study was to investigate the changes in blood activity during rest, exercise and recovery, and to assess its influence on left ventricular (LV) volume determination using the count-based method requiring blood sampling. Methods: Forty-four patients underwent rest-stress radionuclide ventriculography; Tc-99m-human serum albumin was used in 13 patients (Group I), red blood cells was labeled using Tc-99m in 17 patients (Group II) in vivo, and in 14 patients (Group III) by modified in vivo/in vitro method. LV volumes were determined by a count-based method using corrected count rate in blood samples obtained during rest, peak exercise and after recovery. Results: In group I at stress, the blood activity decreased by 12.6 ± 5.4%, p <0.05, as compared to the rest level, and increased by 25.1 ± 6.4%, p <0.001, and 12.8 ± 4.5%, p <0.05, above the resting level in group II and III, respectively. This had profound effects on LV volume determinations if only one rest blood aliquot was used: during exercise, the LV volumes significantly decreased by 22.1 ± 9.6%, p <0.05, in group I, whereas in groups II and III it was significantly overestimated by 32.1 ± 10.3%, p <0.001, and 10.7 ± 6.4%, p <0.05, respectively. The changes in blood activity between stress and recovery were not significantly different for any of the groups. Conclusion: The use of only a single blood sample as volume aliquot at rest in rest-stress studies leads to erroneous estimation of cardiac volumes due to significant changes in blood radioactivity during exercise and recovery.

Antioxidant activities and lipid peroxidation status in human follicular fluid: age-dependent change

Zygote ◽  
2021 ◽  
pp. 1-5
Author(s):  
H. Debbarh ◽  
N. Louanjli ◽  
S. Aboulmaouahib ◽  
M. Jamil ◽  
L. Ahbbas ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Follicular Fluid ◽  
Maternal Age ◽  
Antioxidant Activities ◽  
Human Follicular Fluid ◽  
Age Related ◽  
Group I ◽  
Group Ii ◽  
Scavenging Efficiency

Summary Maternal age is a significant factor influencing in vitro fertilization (IVF) outcomes. Oxidative stress (OS) is one of the major causes of age-related cellular and molecular damage. The purpose of this work was to investigate the correlation between maternal age with intrafollicular antioxidants and OS markers in follicular fluid (FF), and also to determine the OS status in patients of advanced age. This study was a prospective study including 201 women undergoing IVF whose age was between 24 and 45 years old. FF samples were obtained from mature follicles at the time of oocyte retrieval. After treatment of FF, lipid peroxidation levels (MDA) and enzyme activities such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione (GSH) level were evaluated using spectrophotometry. The results indicated that the age cutoff point for increasing the MDA level was fixed at 37 years, allowing the study to be differentiated into two age groups. Group I included patients whose age was less than 37 years, and group II included patients whose age was greater than or equal 37 years. Statistical analysis revealed that MDA and GSH levels and GR activity were significantly higher in group II compared with group I. The SOD and CAT activities were significantly less in group II compared with group I. We concluded that from 37 years old a reproductive ageing was accompanied by a change in the antioxidant pattern in FF that impaired reactive oxygen species scavenging efficiency.

Comparative analysis of the effectiveness of surgical techniques for the treatment of Miller Class I gingival recessions

2021 ◽  
Vol 26 (2) ◽  
pp. 150-157
Author(s):  
R. R. Farkhshatova ◽  
L. P. Gerasimova ◽  
I. T. Yunusov
Keyword(s):  
Connective Tissue ◽  
Soft Tissues ◽  
Platelet Rich Plasma ◽  
Class I ◽  
Study Group ◽  
Connective Tissue Graft ◽  
Group I ◽  
Autologous Platelet Rich Plasma ◽  
Group Ii ◽  
Autologous Platelet

Relevance. It is currently relevant to study and compare the effectiveness of the autologous connective tissue grafts and the combination of collagen-based and autologous platelet-rich plasma in the surgical treatment of Miller Class I gingival recessions.Materials and methods. We examined and treated 48 (20 male (41.67%) and 28 female (58.33%)) patients aged from 25 to 40 years with Miller Class I gingival recessions. All gingival recessions were treated surgically using a modified twolayer tunnel technique. The patients were divided into two groups according to the graft type. Group I (24 patients (50%) had a connective tissue graft from the hard palate. Group II (24 patients (50%) used the combination of the autologous platelet-rich plasma and 3D collagen matrix Fibromatrix for the regeneration of oral soft tissues. We removed the sutures on the 14th day. The patients were followed up on the 7th and 14th days and in 1.3 months.Results. 48 Miller Class I gingival recessions were treated between 2018 and 2020. The depth of gingival recessions averaged 3.5 ± 1.13 mm before treatment. The level of the attached keratinized gingiva regarding the cementoenamel junction significantly (p < 0.001) improved in both groups after the surgery. The width and thickness of the keratinized gingiva best increased in group II. The mean effectiveness of gingival recession treatment was 84% in study group I and 96% – in study group II. Pain syndrome, fibrinous plaque and soft tissue edema were insignificant in group II.Conclusion. The combination of the autologous platelet-rich plasma and Fibromatrix, collagen 3D matrix, for the regeneration of the oral soft tissues is a more effective technique for the treatment of Miller Class I gingival recessions. This technique has several advantages. It is minimally invasive, less painful, soft tissue postoperative swelling is less and the received volume of the attached keratinized gums is larger than with a connective tissue graft. 

317 Effect of the Whole Cottonseed with Pre-treatment on the Digestibility, Productivity, and Milk Fat Profile of Dairy Cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 172-173
Author(s):  
Xiaoge Sun ◽  
Yitong Su ◽  
YangYi Hao ◽  
Gaokun Liu ◽  
Yue Gong ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Milk Fat ◽  
Alkali Treatment ◽  
Mixed Alkali ◽  
Group I ◽  
Animal Feeding ◽  
Group Ii ◽  
Whole Cottonseed

Abstract To improve the efficiency of whole cottonseed (WCS) on dairy cattle, crush and alkali treatment were combined (Table 1) in this study. The gastrointestinal tract digestibility of WCS were measured by in situ ruminal incubation and in vitro fermentation, respectively. Finally, animal feeding experiment was conducted to evaluate the effect of processed WCS on dairy cattle. A total of 30 Holstein dairy cows with similar physiological status were allocated to three groups (CON, Group I, and Group II). Ration for the CON group was a basic ration with no WCS, ration with about 8% non-processed WCS was for Group I (DM basis), while the Group II was provided by the ration added with 8% Crush-Alkali (4% mixed alkali solids with NaOH/CaO ratio at 1:1) treated WCS. Results of the in situ and in vitro experiments showed that, compared with non-treated WCS, Crushing-Alkali treatment groups significantly improved WCS ruminal effective degradation rate of dry matter (DM), crude protein (CP), ether extract (EE), and neutral detergent fiber (NDF) (P &lt; 0.05). Furthermore, Crush-Alkali (4% mixed alkali solids with NaOH/CaO ratio at 1:1) was the most beneficial to improve WCS gastrointestinal degradation of DM, CP and NDF, as well as enhance WCS rumen-bypass of EE. With the lowest free gossypol content (P&lt; 0.05). Results of animal feeding experiment showed that DMI, 4% fat corrected milk production (4% FCM), milk protein, milk fat, and content of short-chain saturated fatty acids in milk in Group II significantly increased (P &lt; 0.05) compared with CON group. DMI, ω-6 polyunsaturated fatty acids (PUFA) in milk, and the ratio of ω-6 to ω-3 PUFA were significantly higher in Group II than that in Group I (P &lt; 0.05). Additionally, free gossypol concentration in serum and milk, as well as parameters reflecting liver function were not notably different among the three groups (P &gt; 0.05).

scholarly journals Assessment of shear bond strength of brackets bonded by direct and indirect techniques: an in vitro study

2012 ◽  
Vol 17 (4) ◽  
pp. 1-7 ◽  
Author(s):  
Roberto Hideo Shimizu ◽  
Karlos Giovani Grando ◽  
Isabela Almeida Shimizu ◽  
Augusto Ricardo Andriguetto ◽  
Ana Cláudia Moreira Melo ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Adhesive System ◽  
Vitro Study ◽  
Group Iii ◽  
Indirect Bonding ◽  
Group I ◽  
Group Ii

OBJECTIVE: This in vitro study was designed to evaluate the shear bond strength (SBS) of orthodontic metal brackets bonded by direct and indirect techniques. METHODS: Thirty healthy human maxillary premolar teeth were used. The teeth were divided into three groups of 10 teeth each: Group I - indirect bonding with SondhiTM Rapid-Set system (3M/Unitek), Group II - indirect bonding with TransbondTM XT adhesive system (3M/Unitek) and Group III - direct bonding with TransbondTM XT adhesive system (3M/Unitek). After bonding and obtaining the specimens for the study, the specimens were subjected to SBS testing in a universal testing machine (Emic, model DL-500). The Kolmogorov-Smirnov test was applied to ascertain that the data had a normal distribution and the Bartlett test to check whether there was homogeneity of variance. One-factor analysis of variance was performed and, subsequently, Tukey's test for paired means. A 5% significance level was adopted. RESULTS: The results of Group I were 67.6 (N) and 5.9 (MPa); Group II, 68.9 (N) and 6.1 (MPa) and Group III (control), 92.5 (N) and 8.1 (MPa). CONCLUSION: It can therefore be concluded that the means for Group III were significantly higher compared with Groups I and II in both Newton (N) and Megapascal (MPa) values. The means attained by the indirect bonding technique used in Groups I and II, however, exhibited no statistically significant differences.

Effects of Cyclic Stress on the Mechanical Properties of Cultured Collagen Fascicles from the Rabbit Patellar Tendon

2003 ◽  
Vol 125 (6) ◽  
pp. 893-901 ◽  
Author(s):  
Ei Yamamoto ◽  
Susumu Tokura ◽  
Kozaburo Hayashi
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Patellar Tendon ◽  
Control Level ◽  
Quadratic Function ◽  
Peak Stress ◽  
Cyclic Stress ◽  
Original Strength

Effects of cyclic stress on the mechanical properties of collagen fascicles were studied by in vitro tissue culture experiments. Collagen fascicles (approximately 300 μm in diameter) obtained from the rabbit patellar tendon were applied cyclic load at 4 Hz for one hour per day during culture period for one or two weeks, and then their mechanical properties were determined using a micro-tensile tester. There was a statistically significant correlation between tensile strength and applied peak stress in the range of 0 to 5 MPa, and the relation was expressed by a quadratic function. The maximum strength (19.4 MPa) was obtained at the applied peak stress of 1.8 MPa. The tensile strength of fascicles were within a range of control values, if they were cultured under peak stresses between 1.1 and 2.6 MPa. Similar results were also observed in the tangent modulus, which was maintained at control level under applied peak stresses between 0.9 and 2.8 MPa. The stress of 0.9 to 1.1 MPa is equivalent to approximately 40% of the in vivo peak stress which is developed in the intact rabbit patellar tendon by running, whereas that of 2.6 to 2.8 MPa corresponds to approximately 120% of the in vivo peak stress. Therefore, the fascicles cultured under applied peak stresses of lower than 40% and higher than 120% of the in vivo peak stress do not keep the original strength and modulus. These results indicate that the mechanical properties of cultured collagen fascicles strongly depend upon the magnitude of the stress applied during culture, which are similar to our previous results observed in stress-shielded and overstressed patellar tendons in vivo.

scholarly journals Results of modern tuboperitoneal infertility treatment

2009 ◽  
Vol 66 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Drenka Turjacanin-Pantelic ◽  
Dragana Bojovic-Jovic ◽  
Biljana Arsic ◽  
Eliana Garalejic
Keyword(s):  
Statistical Analysis ◽  
Surgical Treatment ◽  
Infertility Treatment ◽  
P Value ◽  
Modern Approach ◽  
Vitro Fertilization ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

Background/Aim. A modern approach to surgical treatment of tuboperitoneal infertility is based on laporascopic techniques. The aim of this study was to compare results of tuboperitoneal infertility treatment by the use of laparoscopy and classical laparotomy. Methods. A retrospectiveprospective study on 66 women treated operatively form tuboperitoneal infertility was performed. Data from patient's anamnesis and those related to the surgical treatment results, obtained by the use of an inquiry, were used in retrospective and prospective analysis, respectively. Chi-square test was used in statistical analysis. P value < 0.05 was considered significant. Results. Classical laparotomy was used on 34 women in a period from 1996 to 1997, while 32 women were operated laparoscopically in a period from 1999 to 2000. The results were as follows: a total number of conceived women was 16 (24%), seven in the group I (20.6%) and nine in the group II (28.1%); 13 women were with one pregnancy, six in the group I (17.6%) and seven in the group II (22%). Twice pregnant were three women, one in the group I (2.9%) and two in the group II (6.2%). The resulting pregnancies were: five women with abortion spontaneous, two in the group I (5.9%) and three in the group II (9.4%); two women with extrauterine pregnancy in the group I (5.9%); three with pretemporal birth, one in the group I (2.9%) and two in the group II (6.2%), while six women were with the temporal birth, two in the group I (5.9%) and four in the group II (12.5%). Statistical analysis showed that there was no significant difference in the results between these two groups. Conclusion. Surgical treatment of tubeperitoneal infertility, regardless of the used methods (classical laparotomy or laparoscopy) was successful in a great number of women. These methods have a great advantage over in vitro fertilization, and they should not be ignored.

An in-vitro Comparison of the Bleaching Efficacy of 35 Percent Carbamide Peroxide with Established Intracoronal Bleaching Agents

2020 ◽  
Author(s):  
Nandita Bansal
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Perborate ◽  
Distilled Water ◽  
Carbamide Peroxide ◽  
Human Teeth ◽  
Anterior Teeth ◽  
Group I ◽  
Group Ii ◽  
Control Evaluation

Introduction: Intracoronal bleaching is the simplest, least invasive, and least expensive means available to lighten non-vital discolored teeth. The objective of the study: To evaluate the intra coronal bleaching efficacy of 35% Carbamide peroxide relative to 35% Hydrogen peroxide and Sodium perborate using artificially stained extracted human teeth. Materials and Methods: Eighty extracted human permanent maxillary and mandibular anterior teeth and single-rooted premolars were artificially stained using whole human blood and root canal treatment was completed. They were bleached twice 7 days apart using: Group I (n=20) – 35% Carbamide peroxide gel, Group II (n=20) – 35% Hydrogen peroxide gel, Group III (n=20) – 2 gm Sodium perborate mixed with per ml of distilled water, Group IV (n=20)– Distilled water only (control). Evaluation of shade was performed on day 0,7,14. Results: On day 7, In Groups I and Group II, there was a reduction in the shade by 2 vita tab positions, whereas Group IIIlightened by 1-tab position only. On day 14, CP and HP lightened by overall 3-tab positions and SP by 2-tab positions. There were no statistical differences between the groups on day 7 and day 14. Conclusion: 35% Carbamide peroxide, 35% Hydrogen peroxide and Sodiumperborate were equally effective for Intra coronal bleaching.

scholarly journals Are Patients with Polycystic Ovarian Syndrome Ideal Candidates for Oocyte Donation?

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
George Queiroz Vaz ◽  
Alessandra Viviane Evangelista ◽  
Cassio Alessandro Paganoti Sartorio ◽  
Maria Cecilia Almeida Cardoso ◽  
Maria Cecilia Erthal ◽  
...  
Keyword(s):  
Polycystic Ovarian Syndrome ◽  
Oocyte Donation ◽  
Good Choice ◽  
Pregnancy Rates ◽  
Egg Donation ◽  
Vitro Fertilization ◽  
Group I ◽  
Group Ii ◽  
Ovarian Syndrome

Background.The use of donated oocytes for in vitro fertilization treatment in patients with ovarian failure is universally recognized. But would patients with polycystic ovarian syndrome (PCOS) be a good choice for egg donation programs?Objective.Comparing the pregnancy rates of egg receptors from donor patients diagnosed with PCOS to receptors from donors without PCOS.Design.Retrospective cohort study.Methods.A total of 234 patients who had undergone egg reception program were separated into two groups: Group I, receptors from PCOS donors (n=36); Group II, receptors from donors without PCOS (n=198). Medical records were reviewed and the fertilization, implantation, and pregnancy rates were calculated.Results.PCOS patients had an average of 3.23 more oocytes retrieved, but there were no differences in the number of mature oocytes that were used for donation between the groups. We also observed that the number of transferred embryos was also not significantly different, as well as the fertilization and implantation rates. The clinical pregnancy rates were not significantly different: 28% and 26% in Group I and Group II, respectively.Conclusions.Women with PCOS should not be excluded from egg donation programs.

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