scholarly journals The Effect of Commercially Available Desensitizers on Bond Strength Following Cementation of Zirconia Crowns Using Self-Adhesive Resin Cement—An In Vitro Study

Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 514
Author(s):  
Harisha Dewan ◽  
Mohammed E. Sayed ◽  
Nasser M. Alqahtani ◽  
Tariq Alnajai ◽  
Abdulaziz Qasir ◽  
...  
Keyword(s):  
Tensile Strength ◽  
In Vitro Study ◽  
Resin Cement ◽  
Control Group ◽  
P Value ◽  
Dentin Hypersensitivity ◽  
Luting Cement ◽  
Zirconia Crowns ◽  
Group 2

The improvement of the tensile strength of zirconia crowns after the application of commercially available desensitizers can provide added advantages for the durability and strength of zirconia prostheses. We assessed the retention of zirconia crowns when Gluma, Shield Force Plus, and Telio CS desensitizers were used with resin luting cement. Four groups with ten specimens each (n = 10) were considered as Group 1 (Control group, with no desensitizer application before crown cementation with resin cement) and Groups 2, 3, and 4 (with a single coat of Gluma dentin desensitizer, Telio CS desensitizer, or Shield Force Plus desensitizer applied before crown cementation, respectively). Thermocycling was then carried out, and each group was tested to determine the associated retentive forces and type of failure. The data were statistically analyzed, which showed that the mean tensile-strength values were significantly higher in Group 2 (p-value = 0.001), Group 3 (p-value = 0.027), and Group 4 (p-value = 0.014), when compared with the Control group. Clinicians should consider the application of any of these three desensitizers, as they can successfully abate dentin hypersensitivity after tooth preparation, as well as increase the durability and strength of the zirconia prosthesis.

Evaluation of physical and adhesive properties of enamel after a therapeutic dose of radiation and bonding of orthodontic metal brackets: an in vitro study

2020 ◽  
pp. 1-5
Author(s):  
A. Anushree ◽  
Ashutosh Shetty ◽  
Crystal Runa Soans ◽  
M. N. Kuttappa ◽  
Akhil Shetty ◽  
...  
Keyword(s):  
Testing Machine ◽  
In Vitro Study ◽  
Tooth Surface ◽  
Control Group ◽  
Adhesive Properties ◽  
Adhesive Resin ◽  
Adhesive Remnant Index ◽  
Group 3 ◽  
Group 2

Abstract Aim: The study aimed to evaluate the shear bond strength (SBS) of metal brackets and adhesive properties of bonded irradiated and non-irradiated teeth. Methods: Sixty-six extracted premolar samples were randomly divided into three groups—(a) Control group consisting of 22 non-irradiated, non-aged teeth (Group 1), (b) 22 non-irradiated, aged samples (Group 2) and (c) 22 irradiated, aged samples (Group 3). Irradiation was done using gamma irradiation with a fractionated dose of 60 Gy for 5 consecutive days per week over 6 weeks. Metal brackets were bonded on all samples with light cure adhesive and subjected to SBS test using universal testing machine. The samples were assessed under the scanning electron microscope to check for the adhesive remnant index (ARI) and tag depth. Results: There was a statistically significant decrease in the mean SBS of the irradiated samples compared to the non-irradiated teeth. The non-irradiated, aged samples showed a majority of ARI scoring 1 and 2. Whereas, the irradiated samples showed ARI scoring 2 and 3. Approximately, 77·3% of the non-irradiated samples showed no adhesive present on the tooth surface, and 27·2% of the irradiated samples had more than 50% adhesive present on the enamel surface. Conclusion: There is a statistically significant decrease in SBS of irradiated enamel compared to that of non-irradiated teeth. However, the SBS observed in the three groups was well above the ideal SBS for orthodontic bonding, that is, 5·6–6·8 MPa. The adhesive remnant was found on all samples of the irradiated group. Deeper adhesive resin tags were found in the irradiated group in the resin–enamel interface.

scholarly journals Analysis of Surface Roughness and 3D Scanning Topography of Zirconia Implants Before and After Photofunctionalisation by 3D Optical Profilometry- An Invitro Study

2021 ◽  
Author(s):  
Arunjaikumar Ravindran ◽  
Suma Karthigeyan ◽  
Ramesh Bhat ◽  
Madhulika Naidu ◽  
Senthilnathan Natarajan ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Roughness Parameter ◽  
In Vitro Study ◽  
Control Group ◽  
P Value ◽  
Study Group ◽  
Optical Profilometry ◽  
Before And After ◽  
Zirconia Implants

Introduction: Zirconia is considered as an alternative material for the fabrication of implants. Surface roughness of the implant plays a fundamental role in the initial bone formation. The rationale of using 3D optical profilometry in this study was to evaluate the surface roughness of the zirconia implants before and after Ultraviolet (UV) photofunctionalisation. Also, Moreso 3D optical instruments have a better resolution than the mechanical ones like Atomic Force Microscopy (AFM). Aim: To analyse the surface roughness and topography of zirconia implants after photofunctionalisation using optical profilometry. Materials and Methods: This is an in-vitro study conducted over a period of six months from March 2020 to August 2020. Ten commercially machined Zirconia implants, five each in study and control group, were micro analysed at three different regions (abutment, thread and crest) by optical profilometry. Study group was surface treated by UV radiation for 48 hours. Quantitative morphometric analysis was done between two groups and p-value less than 0.05 was considered statistically significant. The statistical test applied in this study was independent t-test. Results: Scanning micrographs of the study group revealed highest density of summits contributing to increased surface area in the study group. Quantitative analysis of surface roughness showed statistically significant higher mean roughness parameter for photofunctionalised implants in abutment, crest and thread region (p<0.05). Conclusion: Photofunctionalisation is a potentially synergistic technique in producing textured zirconia implants.

scholarly journals Fracture Resistance of Primary Molars Restored with Endocrowns Versus Zirconia Crowns (An in Vitro Study)

2021 ◽  
Vol 24 (4) ◽  
Author(s):  
Ayman Sabbah ◽  
Mostafa Kamel
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
In Vitro Study ◽  
Composite Resins ◽  
Primary Molars ◽  
P Value ◽  
Pulp Chamber ◽  
Group I ◽  
Zirconia Crowns

Objective: This study aimed to assess the fracture resistance of primary molars restored with endocrowns compared to prefabricated zirconia crowns. Methods: Twelve sound, defect-free primary second molars were selected and divided into two groups: group I included 6 molars restored with zirconia crowns, while group II included 6 teeth restored with endocrowns. For both groups access cavity was prepared for the teeth to open pulp chamber. Fracture resistance of both groups was tested using a universal testing machine. Results: Zirconia crown showed statistically significantly higher mean fracture resistance than Endocrown (P-value = 0.001, Effect size = 2.72). Conclusion: Pulpotomized primary molars restored with Zirconia crowns showed significantly higher mean fracture resistance compared to primary molars restored with endocrowns, meanwhile both restorations have shown higher mean fracture resistance than the maximum biting force in children. Keywords  Composite resins; Endocrown; Fracture strength; Zirconia crowns.

scholarly journals Effect of lithium disilicate ceramic thickness, shade and translucency on transmitted irradiance and knoop microhardness of a light cured luting resin cement

2021 ◽  
Vol 32 (8) ◽  
Author(s):  
Lincoln Pires Silva Borges ◽  
Gilberto Antônio Borges ◽  
Américo Bortolazzo Correr ◽  
Jeffrey A. Platt ◽  
Sidney Kina ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Lithium Disilicate ◽  
Resin Cement ◽  
Control Group ◽  
Ceramic Disc ◽  
All Ceramic ◽  
Light Curing ◽  
Knoop Microhardness ◽  
Ceramic Thickness

AbstractThis in vitro study evaluates the influence of pressed lithium disilicate thickness, shade and translucency on the transmitted irradiance and the Knoop microhardness (KHN) of a light-cured resin cement at two depths. One hundred and thirty-five ceramic discs of IPS e.max Press (Ivoclar Vivadent) were fabricated and divided into twenty-seven groups (n = 5) according to the association between translucency: HT (hight translucency), LT (low translucency), and MO (medium opacity); shade: BL2, A1 and A3.5; and thickness: 0.5 mm, 1.5 mm, and 2.0 mm. One side of each ceramic disc was finished, polished and glazed. The irradiance (mW/cm²) of a multiwave LED light curing unit (Valo, Ultradent) was evaluated with a potentiometer (Ophir 10ª-V2-SH, Ophir Optronics) without (control group) or with interposition of ceramic samples. The microhardness of Variolink Esthetic LC resin cement (Ivoclar Vivadent) was evaluated after 24 h at two depths (100 μm and 700 μm). Data were submitted to ANOVA followed by Tukey’s test (α = 0.05). Irradiance and KHN were significantly influenced by ceramic thickness (p < 0.0001), shade (p < 0.001), translucency (p < 0.0001) and depth (p < 0.0001). Conclusions: the interposition of increasing ceramic thicknesses significantly reduced the irradiance and microhardness of resin cement. Increased depth in the resin cement showed significantly reduced microhardness for all studied groups. Increased ceramic opacity reduced the KHN of the resin cement at both depths for all ceramic thicknesses and shades.

scholarly journals A Comparison of Force Decay between Coil Spring, Elastomeric Chain and Tie-backs in Various Alcohol Concentrations found in Mouth Rinse: An In-vitro Study

2021 ◽  
pp. 155-162
Author(s):  
Asma Fatima ◽  
Prasad Konda ◽  
Asiya Fatima ◽  
Hidayathulla Shaikh ◽  
Butool Zohra ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Tooth Movement ◽  
Artificial Saliva ◽  
Alcohol Exposure ◽  
Control Group ◽  
P Value ◽  
Coil Spring ◽  
Mouth Rinses ◽  
Force Decay

Background: The ability to close space efficiently in Orthodontics is of major clinical importance. Elastomeric power chain, coil spring, and tiebacks are commonly used in Orthodontics to achieve tooth movement during the closure of spaces. Many mouth rinses which are used by the patients to achieve good oral hygiene affect the properties of the material used during treatment resulting in force decay if they contain alcohol. Aim: To know the effect of mouth rinses containing different alcohol Concentrations on the force decay of retraction materials. Materials and Methods: A study was carried out to test the effect of alcohol exposure found in mouth rinses on orthodontic NiTi closing coils, elastomeric chains, and tie-back. A total of 135 specimens were divided into one control group and two test groups submerged in artificial saliva at 37⁰ C. Two test groups each of them exposed to different alcohol-containing mouthwashes (Listerine and Povidone-iodine) for 60 seconds twice a day and the control group were exposed only to deionized (DI) water for 28 days. Force measurements were taken at six-time points (initial, 7 days, 14 days, 21 days, and 28 days) using a digital force gauge. Results: The comparison between the tensile strength was made by digital force gauge and the p-value (≤ 0.05) for tensile strength was derived by ANOVA test, multiple comparisons, and Tukey’s correction. Significant force decay was seen in test groups when compared to the control group. Conclusion: Force degradation of retraction products used during orthodontic treatment was effected by mouth rinses containing alcohol.

scholarly journals In Vitro Evaluation of the Effect of Different Luting Cements and Tooth Preparation Angle on the Microleakage of Zirconia Crowns

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Behnaz Ebadian ◽  
Amirhossein Fathi ◽  
Melika Savoj
Keyword(s):  
Resin Cement ◽  
Cement Type ◽  
Digital Photograph ◽  
Luting Cements ◽  
Luting Cement ◽  
Cad Cam ◽  
Zirconia Crowns ◽  
Tooth Preparation ◽  
Cementing Agent

Introduction. Discrepancy between the crown border and prepared tooth margin leads to a microleakage that eases the penetration of microorganisms and causes the dissolution of luting cement consequently. Several factors should be considered to achieve optimal fitness, including tooth preparation taper and type of cementing agent. The study aimed to determine the relation of tooth preparation taper and cement type on the microleakage of zirconia crowns. Materials and Methods. Fifty-six freshly extracted premolars without caries and restorations were selected as the study sample and divided into two groups of different tapering degrees (6 and 12 degrees). Zirconia copings were designed and fabricated by the CAD/CAM system. The samples were divided into four subgroups for cementation, and each subgroup was cemented with a different luting cement (n = 7). After 5000 thermocycles at 5°C–55°C and dye penetration, the specimens were sectioned in the mid-buccolingual direction, and a digital photograph of each section was taken under a stereomicroscope. Data were analyzed by the Kruskal–Wallis and Mann–Whitney tests (α = 0.05). Results. The results showed significant differences among the four types of luting cement in marginal permeability (PV < 0.001). Regardless of the type of cement, the 12-degree tapering resulted in a lower microleakage (46.4% without microleakage) with statistically significant differences from the 6-degree tapering (PV = 0.042). Conclusion. Within the limitations of this study, increasing the tapering degree of the prepared tooth for CAD/CAM zirconia copings improved the marginal fit and decreased the microleakage score. In addition, total-etch resin cement indicated the least microleakage.

scholarly journals Evaluation of equine corneal endothelium after exposure to 0.05% brilliant blue - an in vitro study

2019 ◽  
Vol 71 (4) ◽  
pp. 1158-1164
Author(s):  
M.C.C. Andrade ◽  
T.M. Moreno ◽  
M.S. Muccillo ◽  
J.A.T. Pigatto ◽  
E.V. Camilo
Keyword(s):  
Corneal Endothelium ◽  
In Vitro Study ◽  
Brilliant Blue ◽  
Control Group ◽  
Chi Square ◽  
Uniform Size ◽  
Chi Square Test ◽  
Group 2 ◽  
Scanning Electron

ABSTRACT The aim of this study was to evaluate the immediate effects of 0.05% brilliant blue on corneal endothelium of horses. Thirty-eight corneas of 19 horses, male or female, of different ages were studied. Corneas were randomly divided into two groups. Group 1: Corneal endothelium was covered with 0.3mL of brilliant blue 0.05% for 60 seconds followed by rinsing with a balanced salt solution. Group 2: Corneal endothelium was covered with BSS for 60 seconds. The corneas were excised with an 8mm trephine and prepared to analyze posterior endothelial surface using a light microscope (24 corneas) and a scanning electron microscope (14 corneas). The equine posterior corneal endothelium surface observed by optical microscopy and scanning electron microscopy revealed a continuous layer of polygonal cells of uniform size and shape in both the control and treatment groups. Due to non-normal residuals at ANOVA mean comparison, a generalized linear model was utilized at 5% level of significance. The chi-square test stated that treatment and control group were not different statistically. The 0.05% brilliant blue did not cause damage to equine corneal endothelium.

Comparison of the Effects of Cytotoxicity and Antimicrobial Activities of Self-adhesive, Eugenol and Noneugenol Temporary and Traditional Cements on Gingiva and Pulp Living Cells

2019 ◽  
Vol 10 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Murat Alkurt ◽  
Zeynep Yesil Duymus ◽  
Tugay Sisci
Keyword(s):  
Cell Viability ◽  
Brain Heart Infusion ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Resin Cement ◽  
Control Group ◽  
Pulp Cell ◽  
Luting Cement ◽  
Agar Diffusion Test

Aims and objectives: The aim of this in vitro study was to investigate the antibacterial and cytotoxic effect of cements: zinc polycarboxylate, glass ionomer, self-adhesive resin cement, eugenol-containing and eugenol-free temporary cements. Materials and methods: The agar-diffusion test was carried out on 4 types of bacteria: Streptococcus mutans ( S. mutans; ATCC 35668), Streptococcus salivarius ( S. salivarius; ATCC 13419), Streptococcus sangius ( S. sangius; ATCC 10556), and Lactobacillus casei ( L. casei; ATCC 27139). Freshly prepared cement samples were placed on a brain heart infusion medium and left at 37°C for 24 hours in a CO2 incubator. Ampicillin disks of 10 mg were used as positive controls. Antimicrobial effects were determined using the zone of inhibition measurement in millimeters at 24 and 48 hours. Cytotoxicity was assessed through a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (SERVA Electrophoresis GmbH, Heidelberg, Germany). To evaluate cell viability, an optical density microplate reader (Tecan™ Sunrise, Männedorf, Switzerland) was used at 24 and 48 hours at 570 and 630 nm, respectively. Results: Except the positive control group, the Adhesor luting cement showed a higher inhibitory effect on S. sangius and L. casei. RelyX Temp E temporary cement showed an inhibitory effect on S. sangius and L. casei, whereas the Cavex temporary cement showed an inhibitory effect on only S. sangius at 24 and 48 hours. At 100% concentration, Cavex temporary cement showed the least toxicity (23.6% pulp cell and 9.9% gingival cell) and highest cell viability (76.4% pulp cell and 90.1% gingival cell) at 24 hours. However, Meron, Adhesor, Panavia SA luting, and RelyX Temp E temporary cements showed the highest toxicity (above 94% gingival cell and 95% pulp cell) at 100% concentration. Conclusions: Meron, Adhesor, Panavia SA luting, and RelyX Temp E temporary cements may have a cytotoxic potential. None of the cements showed an inhibitory effect on S. mutan and S. salivarius, whereas Adhesor luting cement has a higher inhibitory effect than that of S. sangius and L. casei.

scholarly journals Pulp tissue dissolution capacity of QMix 2in1 irrigation solution

2015 ◽  
Vol 09 (03) ◽  
pp. 423-427 ◽  
Author(s):  
Dilara Arslan ◽  
Mehmet Burak Guneser ◽  
Alper Kustarci ◽  
Kursat Er ◽  
Seyda Herguner Siso
Keyword(s):  
Saline Solution ◽  
In Vitro Study ◽  
Control Group ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Group 2

ABSTRACT Objective: The aim of this study was to evaluate the tissue dissolution efficacy of four root canal irrigation solutions (sodium hypochlorite [NaOCl], chlorhexidine gluconate [CHX], Octenidine [OCT], and QMix 2in1) on bovine pulp tissue. Materials and Methods: Fifty bovine pulp tissue samples, each weighing 6.55 mg, were prepared and randomly divided into four experimental groups and one control group (n = 10) according to the dissolution irrigants used: (1) 5.25% NaOCl group; (2) 2% CHX group; (3) OCT group; (4) QMix 2in1 group; and (5) control group (saline solution). These samples were then placed into special bovine dentin reservoir models and immersed for 1 h with each test solution (0.1 mL of each) at room temperature. The pulp samples were then blotted dry and weighed again. The percentage of weight loss was calculated. Statistically analyzed with one-way analysis of variance and post-hoc Tukey tests (P = 0.05). Results: Saline solution did not dissolve the bovine pulp tissue. All groups, except OCT, dissolved pulp samples more effectively than the control group (P < 0.05). The highest tissue dissolution was observed in 5.25% NaOCl group (P < 0.05). No statistically significant difference was found between the tissue-dissolving effect between QMix 2in1 and those of 2% CHX. Conclusions: Within the limitations of this in vitro study, NaOCl exhibited the best tissue-dissolving effect out of all solutions tested. CHX and QMix 2in1 were able to dissolve pulp tissue but less than NaOCl. OCT and saline solutions could not exhibit significantly tissue-dissolving effectiveness. This study shown that QMix 2in1 has little capacity to dissolve pulp tissue therefore used alone is not sufficient for this purpose.

scholarly journals Aesculus hippocastanum L. Extract Does Not Induce Fibroblast to Myofibroblast Conversion but Increases Extracellular Matrix Production In Vitro Leading to Increased Wound Tensile Strength in Rats

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1917
Author(s):  
Ivan Kováč ◽  
Nikola Melegová ◽  
Matúš Čoma ◽  
Peter Takáč ◽  
Katarína Kováčová ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tensile Strength ◽  
Smooth Muscle Actin ◽  
In Vitro Study ◽  
Dermal Fibroblasts ◽  
Control Group ◽  
Horse Chestnut ◽  
Sprague Dawley

The ability of horse chestnut extract (HCE) to induce contraction force in fibroblasts, a process with remarkable significance in skin repair, motivated us to evaluate its wound healing potential in a series of experiments. In the in vitro study of the ability of human dermal fibroblasts to form myofibroblast-like cells was evaluated at the protein level (Western blot and immunofluorescence). The in vivo study was conducted on male Sprague-Dawley rats with inflicted wounds (one open circular and one sutured incision) on their backs. Rats were topically treated with two tested HCE concentrations (0.1% and 1%) or sterile water. The control group remained untreated. The incisions were processed for wound tensile strength (TS) measurement whereas the open wounds were subjected to histological examination. On the in vitro level the HCE extract induced fibronectin-rich extracellular matrix formation, but did not induced α-smooth muscle actin (SMA) expression in dermal fibroblasts. The animal study revealed that HCE increased wound TS and improved collagen organization. In conclusion, the direct comparison of both basic wound models demonstrated that the healing was significantly increased following HCE, thus this extract may be found useful to improve healing of acute wounds. Nevertheless, the use of an experimental rat model warrants a direct extrapolation to the human clinical situation.

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