scholarly journals Production of Lutein-enriched Biomass by Growing Coccomyxa sp. (strain onubensis; Chlorophyta) under Spring Outdoor Conditions of Southwest Spain

2016 ◽  
Vol 6 (1) ◽  
pp. 828-834 ◽  
Author(s):  
Isabel Vaquero ◽  
M. Carmen Ruiz-Domínguez ◽  
Benito Mogedas ◽  
Carlos Vílchez ◽  
José M Vega
Keyword(s):  
Light Intensity ◽  
Large Scale ◽  
Biomass Productivity ◽  
Dry Weight ◽  
Acidic Environment ◽  
Intensity Maximum ◽  
Tubular Photobioreactor ◽  
Outdoor Culture ◽  
Environmental Light ◽  
Intracellular Content

Coccomyxa sp. (strain onubensis) is an acidic environment microalga, which was grown under spring outdoor conditions of Huelva province (southwest, Spain). A 6 L pilot tubular photobioreactor was used to determine the influence of the environmental light and temperature conditions on the microalga growth and its intracellular content of carotenoids. Cloudy days supplied the adequate light intensity (maximum 1070 μmol photons m-2 s-1) and temperature (maximum 32ºC) regimes for a biomass productivity of 0.28 g L-1 d-1 with an intracellular content of lutein (4.8 mg g-1 dry weight) and eventually β-carotene (0.8 mg g-1) obtained at the end of the day evening. This work supplies guidelines for a large-scale outdoor culture of Coccomyxa. sp. to supply lutein-enriched biomass, with the advantage of the growth at pH 2.5, which preserves cultures from contamination.  

scholarly journals Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Marine Drugs ◽  
2019 ◽  
Vol 17 (8) ◽  
pp. 460 ◽  
Author(s):  
Juan Eduardo Sosa-Hernández ◽  
Laura Isabel Rodas-Zuluaga ◽  
Carlos Castillo-Zacarías ◽  
Magdalena Rostro-Alanís ◽  
Reynaldo de la Cruz ◽  
...  
Keyword(s):  
Light Intensity ◽  
Biomass Yield ◽  
Nitrogen Concentration ◽  
Biomass Productivity ◽  
Dry Weight ◽  
High Light Intensity ◽  
High Biomass ◽  
Porphyridium Purpureum ◽  
Nitrogen Concentrations ◽  
Low Nitrogen

Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity.

scholarly journals Cultivating Microalgae in Desert Conditions: Evaluation of the Effect of Light-Temperature Summer Conditions on the Growth and Metabolism of Nannochloropsis QU130

2021 ◽  
Vol 11 (9) ◽  
pp. 3799
Author(s):  
Hareb Al Jabri ◽  
Aumaya Taleb ◽  
Raphaelle Touchard ◽  
Iman Saadaoui ◽  
Vincent Goetz ◽  
...  
Keyword(s):  
Light Intensity ◽  
Large Scale ◽  
Cell Function ◽  
Biomass Productivity ◽  
Interactive Effects ◽  
Cell Membrane Permeability ◽  
Outdoor Cultivation ◽  
Cultivation Conditions ◽  
Light Fluctuations ◽  
The Impact

Temperature and light are two of the most crucial factors for microalgae production. Variations in these factors alter their growth kinetics, macromolecular composition and physiological properties, including cell membrane permeability and fluidity. The variations define the adaptation mechanisms adopted by the microalgae to withstand changes in these environmental factors. In the Qatar desert the temperature varies widely, typically between 10° and 45 °C There are also wide variations in light intensity, with values of over 1500 μmolhν.m−2s−1 in summer. A study of the effects of these thermal and light fluctuations is therefore essential for large-scale outdoor production systems, especially during the summer when temperature and light fluctuations are at their highest. The aim of this work is to study the impact of temperature and light intensity variations as encountered in summer period on the Nannochloropsis QU130 strain, which was selected for its suitability for outdoor cultivation in the harsh conditions of the Qatar desert. It was carried out using lab-scale photobioreactors enabling simulation of both constant and dynamic temperature and light regimes. Biomass productivity, cell morphology and biochemical compositions were examined first in constant conditions, then in typical outdoor cultivation conditions to elucidate the adjustments in cell function in respect of fluctuations. The dynamic light and temperature were shown to have interactive effects. The application of temperature cycles under constant light led to a 13.6% increase in biomass productivity, while a 45% decrease was observed under light and temperature regimes due to the combined stress. In all cases, the results proved that N. sp. QU130 has a high level of adaptation to the wide fluctuations in light and temperature stress. This was shown through its ability to easily change its physiology (cell size) and metabolic process in response to different cultivation conditions.

scholarly journals Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Indrayani Indrayani ◽  
Haslianti Haslianti ◽  
Asmariani Asmariani ◽  
Wellem Muskita ◽  
Abdul Muis Balubi
Keyword(s):  
Growth Rate ◽  
Light Intensity ◽  
High Growth ◽  
Biomass Productivity ◽  
Dry Weight ◽  
High Light Intensity ◽  
High Growth Rate ◽  
Lipid Productivity ◽  
Marine Diatom ◽  
Light Intensities

Abstract. Indrayani I, Haslianti H, Asmariani A, Muskita WH, Balubi M. 2020. Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities. Biodiversitas 21: 1498-1503. Light is one of the important factors affecting growth and biochemical composition of microalgae. The aim of this study was to determine growth, biomass and lipid productivity of a newly isolated marine diatom, Skeletonema sp.UHO29 under different light intensities. The Skeletonema sp. was cultured in 300 mL conical flasks containing 150 mL of f/2 medium under three different light intensities (28, 60 and 110 μmol photons m-2 s-1 ) with three replications. The cultures were incubated under ambient room temperatures, and a cycle of 12 hours light and 12 hours dark, in batch modes for two weeks. The results showed that the highest specific growth rate (0.56 d-1) was achieved at the highest light intensity (110 μmol photons m-2 s-1) and the lowest (0.11 d-1) was obtained at the lowest light intensity (28 μmol photons m-2 s-1). The highest biomass yield (0.62 g L-1) and biomass productivity (0.34 g L-1 d-1) was achieved at the highest light intensity. However, the highest lipid yield (0.175 g L-1), lipid content (28.78% ash-free dry weight) and lipid productivity (0.067 g L-1 d-1) were achieved at medium light intensity. The alga prefers high light intensity for higher growth rate and biomass productivity but it produces more lipids at medium light intensity. This study indicated that the Skeletonema sp.UHO29 is a potential species for outdoor mass cultivation and is a promising microalgal strain for use as a biodiesel feedstock due to its high growth rate, high biomass and lipid productivity.

Fine structure of the retina of the giant scallop, Placopecten magellanicus

1984 ◽  
Vol 42 ◽  
pp. 312-313
Author(s):  
C.V.L. Powell
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Light Intensity ◽  
Scanning Electron Microscope ◽  
Eye Development ◽  
Preliminary Observation ◽  
Columnar Epithelium ◽  
Placopecten Magellanicus ◽  
Environmental Light ◽  
Scanning Electron

The overall fine structure of the eye in Placopecten is similar to that of other scallops. The optic tentacle consists of an outer columnar epithelium which is modified into a pigmented iris and a cornea (Fig. 1). This capsule encloses the cellular lens, retina, reflecting argentea and the pigmented tapetum. The retina is divided into two parts (Fig. 2). The distal retina functions in the detection of movement and the proximal retina monitors environmental light intensity. The purpose of the present study is to describe the ultrastructure of the retina as a preliminary observation on eye development. This is also the first known presentation of scanning electron microscope studies of the eye of the scallop.

scholarly journals Growth of Haematococcus pluvialis on a Small-Scale Angled Porous Substrate Photobioreactor for Green Stage Biomass

2021 ◽  
Vol 11 (4) ◽  
pp. 1788
Author(s):  
Thanh-Tri Do ◽  
Binh-Nguyen Ong ◽  
Tuan-Loc Le ◽  
Thanh-Cong Nguyen ◽  
Bich-Huy Tran-Thi ◽  
...  
Keyword(s):  
Light Intensity ◽  
Algal Biomass ◽  
Haematococcus Pluvialis ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Small Scale ◽  
Porous Substrate ◽  
Low Light ◽  
Green Algal ◽  
Green Stage

In the production of astaxanthin from Haematococcus pluvialis, the process of growing algal biomass in the vegetative green stage is an indispensable step in both suspended and immobilized cultivations. The green algal biomass is usually cultured in a suspension under a low light intensity. However, for astaxanthin accumulation, the microalgae need to be centrifuged and transferred to a new medium or culture system, a significant difficulty when upscaling astaxanthin production. In this research, a small-scale angled twin-layer porous substrate photobioreactor (TL-PSBR) was used to cultivate green stage biomass of H. pluvialis. Under low light intensities of 20–80 µmol photons m−2·s−1, algae in the biofilm consisted exclusively of non-motile vegetative cells (green palmella cells) after ten days of culturing. The optimal initial biomass density was 6.5 g·m−2, and the dry biomass productivity at a light intensity of 80 µmol photons m−2·s−1 was 6.5 g·m−2·d−1. The green stage biomass of H. pluvialis created in this small-scale angled TL-PSBR can be easily harvested and directly used as the source of material for the inoculation of a pilot-scale TL-PSBR for the production of astaxanthin.

scholarly journals Large-scale screening of natural genetic resource in the hydrocarbon-producing microalga Botrycoccus braunii identified novel fast-growing strains

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Koji Kawamura ◽  
Suzune Nishikawa ◽  
Kotaro Hirano ◽  
Ardianor Ardianor ◽  
Rudy Agung Nugroho ◽  
...  
Keyword(s):  
Large Scale ◽  
Biomass Productivity ◽  
Botryococcus Braunii ◽  
Algal Biofuel ◽  
Fast Growing ◽  
Wild Strains ◽  
Glass Tubes ◽  
The Tropics ◽  
Large Scale Screening ◽  
Hydrocarbon Productivity

AbstractAlgal biofuel research aims to make a renewable, carbon–neutral biofuel by using oil-producing microalgae. The freshwater microalga Botryococcus braunii has received much attention due to its ability to accumulate large amounts of petroleum-like hydrocarbons but suffers from slow growth. We performed a large-scale screening of fast-growing strains with 180 strains isolated from 22 ponds located in a wide geographic range from the tropics to cool-temperate. A fast-growing strain, Showa, which recorded the highest productivities of algal hydrocarbons to date, was used as a benchmark. The initial screening was performed by monitoring optical densities in glass tubes and identified 9 wild strains with faster or equivalent growth rates to Showa. The biomass-based assessments showed that biomass and hydrocarbon productivities of these strains were 12–37% and 11–88% higher than that of Showa, respectively. One strain, OIT-678 established a new record of the fastest growth rate in the race B strains with a doubling time of 1.2 days. The OIT-678 had 36% higher biomass productivity, 34% higher hydrocarbon productivity, and 20% higher biomass density than Showa at the same cultivation conditions, suggesting the potential of the new strain to break the record for the highest productivities of hydrocarbons.

scholarly journals Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Bubble Column ◽  
Growth Parameters ◽  
Growth Index ◽  
Dry Weight ◽  
Growth Conditions ◽  
Good Growth ◽  
Gentiana Dinarica ◽  
Hairy Root Clones

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

scholarly journals Potential Use of Organic- and Hard-Rock Mine Wastes on Aided Phytostabilization of Large-Scale Mine Tailings under Semiarid Mediterranean Climatic Conditions: Short-Term Field Study

2012 ◽  
Vol 2012 ◽  
pp. 1-15 ◽  
Author(s):  
Claudia Santibañez ◽  
Luz María de la Fuente ◽  
Elena Bustamante ◽  
Sergio Silva ◽  
Pedro León-Lobos ◽  
...  
Keyword(s):  
Mine Tailings ◽  
Large Scale ◽  
Management Practices ◽  
Hard Rock ◽  
Mine Waste ◽  
Organic Amendments ◽  
Dry Weight ◽  
Climatic Conditions ◽  
Goat Manure ◽  
Site Rehabilitation

The study evaluated the efficacy of organic- and hard-rock mine waste type materials on aided phytostabilization of Cu mine tailings under semiarid Mediterranean conditions in order to promote integrated waste management practices at local levels and to rehabilitate large-scale (from 300 to 3,000 ha) postoperative tailings storage facilities (TSFs). A field trial with 13 treatments was established on a TSF to test the efficacy of six waste-type locally available amendments (grape and olive residues, biosolids, goat manure, sediments from irrigation canals, and rubble from Cu-oxide lixiviation piles) during early phases of site rehabilitation. Results showed that, even though an interesting range of waste-type materials were tested, biosolids (100 t ha-1dry weight, d.w.) and grape residues (200 t ha-1d.w.), either alone or mixed, were the most suitable organic amendments when incorporated into tailings to a depth of 20 cm. Incorporation of both rubble from Cu-oxide lixiviation piles and goat manure into upper tailings also had effective results. All these treatments improved chemical and microbiological properties of tailings and lead to a significant increase in plant yield after three years from trial establishment. Longer-term evaluations are, however required to evaluate self sustainability of created systems without further incorporation of amendments.

scholarly journals Salt-tolerance screening in Limonium sinuatum varieties with different flower colors

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaojing Xu ◽  
Yingli Zhou ◽  
Ping Mi ◽  
Baoshan Wang ◽  
Fang Yuan
Keyword(s):  
Salt Tolerance ◽  
Photosynthetic Rate ◽  
Large Scale ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Salt Gland ◽  
Salt Glands ◽  
Chlorophyll Contents ◽  
Relative Water ◽  
Whole Plants

AbstractLimonium sinuatum, a member of Plumbaginaceae commonly known as sea lavender, is widely used as dried flower. Five L. sinuatum varieties with different flower colors (White, Blue, Pink, Yellow, and Purple) are found in saline regions and are widely cultivated in gardens. In the current study, we evaluated the salt tolerance of these varieties under 250 mmol/L NaCl (salt-tolerance threshold) treatment to identify the optimal variety suitable for planting in saline lands. After the measurement of the fresh weight (FW), dry weight (DW), contents of Na+, K+, Ca2+, Cl−, malondialdehyde (MDA), proline, soluble sugars, hydrogen peroxide (H2O2), relative water content, chlorophyll contents, net photosynthetic rate, and osmotic potential of whole plants, the salt-tolerance ability from strongest to weakest is identified as Pink, Yellow, Purple, White, and Blue. Photosynthetic rate was the most reliable and positive indicator of salt tolerance. The density of salt glands showed the greatest increase in Pink under NaCl treatment, indicating that Pink adapts to high-salt levels by enhancing salt gland formation. These results provide a theoretical basis for the large-scale planting of L. sinuatum in saline soils in the future.

Analyzing and optimizing the carbon utilization and lipid yield at different light intensities for Scenedesmus arcuatus

2021 ◽  
Vol 25 (11) ◽  
pp. 1-10
Author(s):  
K. Vasumathi ◽  
Raja Vadivu G. Nadana ◽  
E.M. Nithiya ◽  
K. Sundar ◽  
M. Premalatha
Keyword(s):  
Light Intensity ◽  
Large Scale ◽  
Biomass Yield ◽  
Osmotic Shock ◽  
High Light Intensity ◽  
Carbon Utilization ◽  
Lipid Yield ◽  
The Face ◽  
Photosynthetic Microorganism ◽  
Pre Treatment

Microalgae, the photosynthetic microorganism growing abundantly in marine and aquatic ecosystems, are potential source for biological sequestration of CO2. The carbon uptake differs in the presence of other nutrients, light intensity etc. The biomass yield of Scenedesmus arcuatus var capitatus was studied based on the Face Centred Central Composite design (FCCD) of Response Surface Methodology (RSM) for nitrate, phosphate and carbonate under different conditions (laboratory, room and sunlight conditions). Various pre-treatments (osmotic shock, autoclaving, microwave and ultrasonication) were employed to find the best method for maximum lipid yield. The biomass yield reached a maximum of 1 g/L under sunlight conditions of nitrate concentration 500 ppm and carbonate 2000 ppm. The laboratory conditions resulted in a biomass yield of 0.59 g/L at 500 ppm nitrate, 1000 ppm carbonate and 250 ppm phosphate. Under room conditions, the yield was very low (0.11 g/L). Osmotic shock resulted in higher lipid yield than the other pre-treatment methods. The ability of Scenedesmus arcuatus to uptake high carbon under sunlight conditions and to adapt to high light intensity and fluctuations in light intensity concludes that this species is suitable for large-scale open pond cultivation for CO2 sequestration and production of metabolites.

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