scholarly journals Effect of Protracted Free-Choice Chlortetracycline-Medicated Mineral for Anaplasmosis Control on Escherichia coli Chlortetracycline Resistance Profile from Pastured Beef Cattle

2021 ◽  
Vol 9 (12) ◽  
pp. 2495
Author(s):  
Alyssa R. Toillion ◽  
Emily J. Reppert ◽  
Raghavendra G. Amachawadi ◽  
K. C. Olson ◽  
Johann F. Coetzee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Treatment Period ◽  
Free Choice ◽  
Current Control ◽  
Control Group ◽  
Fecal Samples ◽  
E Coli ◽  
Control Options ◽  
Cattle Herds

Anaplasmosis is an economically-significant, hemolytic, tick-borne disease of cattle caused by Anaplasma marginale which can cause clinical anemia and death. Current control options are limited, and FDA-approved antimicrobial control options do not have a defined duration of use. A practical and routinely used anaplasmosis control method involves feeding free-choice chlortetracycline (CTC)-medicated mineral to pastured cattle for several months. Constant antimicrobial use poses the risk of expediting the development and dissemination of antimicrobial resistance in off-target commensal bacteria in the bovine gastrointestinal tract. The objective of this study was to determine the CTC-susceptibility of Escherichia coli isolated from anaplasmosis endemic beef cattle herds provided different FDA-approved free-choice CTC-medicated mineral formulations, all intended to provide cattle a dosage of 0.5 to 2.0 mg CTC/lb bodyweight per day. A closed-herd, comprised of Hereford-Angus cows, naturally endemic for anaplasmosis, were grazed in five different pastures with one herd serving as an untreated control group. The other cattle herds were randomly assigned one of four FDA-approved CTC-medicated mineral formulations (700, 5000, 6000, and 8000 g CTC/ton) labeled for “the control of active anaplasmosis” and provided their respective CTC-medicated mineral formulation for five consecutive months. Fecal samples were collected monthly from a subset of cows (n = 6 or 10) per pasture. Fecal samples were cultured for E. coli isolates and the minimal inhibitory concentration of CTC was determined. Baseline CTC-susceptibility of E. coli was variable among all treatment and control groups. The susceptibility of E. coli isolates was significantly different between study herds over the treatment period (p = 0.0037 across time and 0.009 at the final sampling time). The interaction between study herds and treatment period was not significant (p = 0.075).

Comparison of Sampling Methods for Microbiological Testing of Beef Animal Rectal/Colonal Feces, Hides, and Carcasses

2002 ◽  
Vol 65 (4) ◽  
pp. 621-626 ◽  
Author(s):  
J. R. RANSOM ◽  
K. E. BELK ◽  
R. T. BACON ◽  
J. N. SOFOS ◽  
J. A. SCANGA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Sampling Method ◽  
Sampling Methods ◽  
Detection Method ◽  
Escherichia Coli O157 ◽  
Fecal Samples ◽  
E Coli ◽  
Microbiological Testing ◽  
Fecal Sampling

This study compared sampling methods for detecting Escherichia coli O157:H7 and Salmonella in beef cattle feces and on hides and carcasses and for enumerating E. coli biotype I counts (ECC) on carcasses. Fecal samples were collected by rectal/colonal palpation and colonal sponge swabbing. Hides were sampled by sponge swabbing three sites, hair clipping, excision, rinsing, and gauze swabbing, whereas carcasses were sampled by three-site thoracic and pattern-mark sponge swabbing and tissue excision. Overall, irrespective of sampling method, 36.7, 13.3, and 0.0% of lots contained at least one E. coli O157:H7-positive hide, fecal, and carcass sample, respectively, while the corresponding prevalence of Salmonella was 70.0, 16.7, and 6.7%, respectively. For hide sampling, excision and gauze swabbing yielded the fewest (13.3%) E. coli O157:H7-positive samples, while hair clipping and sponge swabbing yielded the most (23.3%). None of the carcass-sampling methods detected E. coli O157:H7 or differed (P > 0.05) in their ability to enumerate ECC. Colonal swabbing was the most effective (10.0%) method for detecting E. coli O157:H7 in feces. No differences (P > 0.05) in Salmonella prevalence were observed between carcass-sampling methods, although three-site sponge swabbing and tissue excision detected the most (3.3%). Hide rinsing was the most effective (P < 0.05) Salmonella detection method (63.3%), but dangers associated with its application may preclude its use by industry; there were no differences (P > 0.05) among other hide-sampling methods. No differences (P > 0.05) in Salmonella detection were observed between fecal-sampling methods. Overall, three-site sponge swabbing was the most feasible and effective sampling method for the detection of E. coli O157:H7 and Salmonella on hides and carcasses.

scholarly journals Effects of Flavophospholipol on Resistance in Fecal Escherichia coli and Enterococci of Fattening P

2002 ◽  
Vol 46 (1) ◽  
pp. 110-118 ◽  
Author(s):  
A. E. van den Bogaard ◽  
M. Hazen ◽  
M. Hoyer ◽  
P. Oostenbach ◽  
E. E. Stobberingh
Keyword(s):  
Escherichia Coli ◽  
Intestinal Flora ◽  
Cross Resistance ◽  
Control Group ◽  
Growth Promoter ◽  
Experimental Conditions ◽  
Fecal Samples ◽  
E Coli ◽  
Vancomycin Resistant ◽  
Group B

ABSTRACT A “plasmid-curing effect” of multiresistant Escherichia coli by flavophospholipol, an antibiotic used as an antimicrobial growth promoter (AMGP) in animal feeds, has been reported to occur in vitro and in vivo under experimental conditions. In this study, the effect of flavophospholipol under field conditions was studied. The prevalence and degree (proportion of resistant strains to the total numbers present per gram of feces) of resistance of indicator bacteria, E. coli and enterococci, was determined in fecal samples from three groups of pigs that were fed a commercial finisher feed without any AMGP. Group A was the negative control group without any AMGP, group B received the same feed with 9 mg of flavophospholipol/kg of feed (study group), and group C received the same feed with 15 mg of avoparcin/kg (positive control). Fecal samples from each pig were collected at the start and at the end of the study and assessed for the prevalence and degree of resistance against antibiotics commonly used either for therapy in pig medicine or as an AMGP. Before the start of the study, all pigs were colonized with multiresistant E. coli by mixing three resistant pig isolates through their feed after disturbance of the colonization resistance of the intestinal flora by a 3-day course of lincomycin and spectinomycin. At the end of the study, the overall prevalence and degree of resistance of E. coli in the fecal flora had increased significantly in groups A and C but remained at the same level as at the start of the study in group B. The prevalence of vancomycin resistance was 44 and 41% in groups A and B, respectively, but only very low numbers of vancomycin-resistant enterococci (VRE) per gram of feces were found. In the avoparcin-fed group, the prevalence was 72%, and in 57% of the samples, more than 50% of all enterococci present were vancomycin resistant. The prevalence of resistant Enterococcus faecalis increased only in the flavophospholipol-exposed group, from 23% before the start of the study to 43% at the end of the study. It was concluded that flavophospholipol effectively suppressed the augmentation and dissemination of multiresistant E. coli in the intestinal flora of fattening pigs. Avoparcin use strongly selected for VRE carriage and excretion. Therefore, as neither flavophospholipol nor any related molecule is used therapeutically, no cross-resistance with therapeutic antibiotics exists and no transmissible resistance has been shown; the major decrease in resistance in intestinal E. coli of flavophospholipol-fed animals seemed to outweigh the small increase in the risk of transfer of flavophospholipol-resistant E. faecalis from animals to humans via the food chain.

scholarly journals Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms

2013 ◽  
Vol 79 (9) ◽  
pp. 3027-3032 ◽  
Author(s):  
A. Schmid ◽  
S. Hörmansdorfer ◽  
U. Messelhäusser ◽  
A. Käsbohrer ◽  
C. Sauter-Louis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Dairy Cows ◽  
Fecal Samples ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Study Population ◽  
Cattle Farms ◽  
Group 1

ABSTRACTExtended-spectrum β-lactamase (ESBL)-producingEscherichia colistrains are believed to be widely distributed among humans and animals; however, to date, there are only few studies that support this assumption on a regional or countrywide scale. Therefore, a study was designed to assess the prevalence of ESBL-producingE. coliin dairy cows and beef cattle in the southern part of Bavaria, Germany. The study population included 30 mixed dairy and beef cattle farms and 15 beef cattle farms. Fecal samples, boot swabs, and dust samples were analyzed for ESBL-producingE. coliusing selective media. PCR was performed to screen for CTX-M andampCresistance genes. A total of 598 samples yielded 196 (32.8%) that contained ESBL-producingE. coli, originating from 39 (86.7%) of 45 farms. Samples obtained from mixed farms were significantly more likely to be ESBL-producingE. colipositive than samples from beef cattle farms (fecal samples,P< 0.001; boot swabs,P= 0.014; and dust samples,P= 0.041). A total of 183 isolates (93.4%) of 196 ESBL-producingE. coli-positive strains harbored CTX-M genes, CTX-M group 1 being the most frequently found group. Forty-six additional isolates containedampCgenes, and 5 of the 46 isolates expressed ablaCMY-2gene. The study shows that ESBL-producingE. colistrains are commonly found on Bavarian dairy and beef cattle farms. Moreover, to our knowledge, this is the first report of the occurrence ofblaCMY-2in cattle in Germany.

National Survey of Shiga Toxin–Producing Escherichia coli Serotypes O26, O45, O103, O111, O121, O145, and O157 in Australian Beef Cattle Feces

2016 ◽  
Vol 79 (11) ◽  
pp. 1868-1874 ◽  
Author(s):  
GLEN E. MELLOR ◽  
NARELLE FEGAN ◽  
LESLEY L. DUFFY ◽  
KATE E. McMILLAN ◽  
DAVID JORDAN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Shiga Toxin ◽  
Immunomagnetic Separation ◽  
The United States ◽  
Pcr Analysis ◽  
Fecal Samples ◽  
E Coli ◽  
Beef Products ◽  
Low Prevalence

ABSTRACT Escherichia coli O157 and six non-O157 Shiga toxin–producing E. coli (STEC) serotypes (O26, O45, O103, O111, O121, and O145, colloquially referred to as the “big 6”) have been classified as adulterants of raw nonintact beef products in the United States. While beef cattle are a known reservoir for the prototype STEC serotype, E. coli O157, less is known about the dissemination of non-O157 STEC serotypes in Australian cattle. In the present study, 1,500 fecal samples were collected at slaughter from adult (n =628) and young (n =286) beef cattle, adult (n =128) and young (n =143) dairy cattle, and veal calves (n = 315) across 31 Australian export-registered processing establishments. Fecal samples were enriched and tested for E. coli O157 and the big 6 STEC serotypes using BAX System PCR and immunomagnetic separation methods. Pathogenic STEC (pSTEC; isolates that possess stx, eae, and an O antigen marker for O157 or a big 6 serotype) were isolated from 115 samples (7.7%), of which 100 (6.7%) contained E. coli O157 and 19 (1.3%) contained a big 6 serotype. Four of the 115 samples contained multiple pSTEC serotypes. Among samples confirmed for big 6 pSTEC, 15 (1%) contained E. coli O26 and 4 (0.3%) contained E. coli O111. pSTEC of serotypes O45, O103, O121, and O145 were not isolated from any sample, even though genes indicative of E. coli belonging to these serotypes were detected by PCR. Analysis of animal classes revealed a higher pSTEC prevalence in younger animals, including veal (12.7%), young beef (9.8%), and young dairy (7.0%), than in adult animals, including adult beef (5.1%) and adult dairy (3.9%). This study is the largest of its kind undertaken in Australia. In contrast to E. coli O157 and consistent with previous findings, this study reports a relatively low prevalence of big 6 pSTEC serotypes in Australian cattle populations.

scholarly journals An Intramuscular Multivalent Vaccine Against Pathogenic Escherichia Coli Injected in Suckling Piglets Reduced the Prevalence of Pathogenic E. Coli and Lawsonia Intracellularis Until Slaughter

2021 ◽  
Author(s):  
Lívia Mendonça Pascoal ◽  
Sarah Rodrigues Chagas ◽  
Francisco J. Pallarés ◽  
Juan J. Quereda ◽  
Juan Manuel Herrero Medrano ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Intestinal Mucosa ◽  
Animal Health ◽  
Control Group ◽  
Lawsonia Intracellularis ◽  
Colon Tissue ◽  
Fecal Samples ◽  
E Coli ◽  
Suckling Piglets

Abstract Background: The present study aims to evaluate the efficacy of an intramuscular multivalent Escherichia coli vaccine for suckling piglets against infection not only by pathogenic E. coli but also by pathogens involved in Porcine Enteric Disease Complex (PEDC). Vaccinated Group had piglets vaccinated at days 10 and 20 of life with Colidex-C® (Vetia Animal Health, Spain), and Control Group had piglets that received sterile saline solution injection at the same days of life. We collected fecal samples in the farm from animals presenting diarrhea and intestinal mucosa swabs and ileum and colon tissue at slaughter and then performed PCR to identify E. coli virulence factors genes. Furthermore, we performed PCR to identify Lawsonia intracellularis, Brachyspira hyodisenteriae, and Salmonella spp.Results: Regarding fecal samples, 0% from Vaccinated Group was positive for E. coli, while Control Group had 94.1% of positive samples (p<0.0001). With respect to intestinal mucosa swab, 0% of the samples from Vaccinated Group were positive for E. coli, while 100% from Control Group were positive (p=0.001). Regarding ileum and colon tissue samples, 35% were positive for E. coli in Vaccinated Group and 85% in Control Group (p=0.001); Gcnt had a higher frequency of F41 (p=0.018), LT (p=0.018) and Sta (p=0.028) virulence factors genes. No sample was positive for Salmonella spp. nor for B. hyodisenteriae, but there were positive samples L. intracellularis; real-time PCR was performed and the frequencies found were 40% and 20% of ileum and colon positive samples in Vaccinated Group and 100% for ileum and 70% for colon in Control Group (p<0.001 for ileum and p=0.001 for colon).Conclusion: The results indicate that the E. coli vaccine for piglets may be a strategy to control E. coli infection. E. coli vaccines emerge as a probable strategy to help control L. intracellularis and, maybe, other enteric pathogens of pigs not evaluated in this study.

scholarly journals Antimicrobial resistance pattern of Escherichia coli strains isolated from poultry farmers and poultry slaughterers in Morocco

10.3823/820 ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
CHAIBA Abdellah ◽  
Rhazi Filali Fouzia
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Antimicrobial Agents ◽  
Resistance Pattern ◽  
Control Group ◽  
Fecal Samples ◽  
E Coli ◽  
Poultry Workers ◽  
Poultry Farmers

Aim: The objective of this study is to characterize the prevalence of antibiotic resistance in Escherichia coli isolates from the fecal samples of poultry workers, and to study the possible dissemination of resistant E. coli  from  poultry to humans. Methodology: Sixty four E. coli strains isolated from the fecal samples of poultry workers and 35 isolates from a control group workers were tested for antibiotic resistance by agar disk diffusion with 11 antimicrobial agents. Results: Resistance of E. coli isolated from poultry workers to tetracycline, ampicillin and norfloxacin were significantly (p < 0,05) higher than those isolated from the control group. All E. coli isolates were susceptible to cefotaxime, and most of them are susceptible to gentamycin, amikacin, cefoxitin and ertapenem. Multidrug resistance is alarmingly high in all groups, but was highest in poultry farmers isolates (84%) and poultry slaughterers isolates (80%). Approximately 25 % of the isolates of poultry workers showed resistance to four or more antibiotics. Conclusion: This study implies  that occupational exposure to antimicrobial-resistant E. coli from animal contact in the broiler chicken industry may be an important route of entry for antimicrobial-resistant E. coli into the community. Keywords : Escherichia coli ; Poultry Workers ; Antibiotic Resistance ; Multidrug Resistance ; Morocco.

scholarly journals Genomic evolution of antimicrobial resistance in Escherichia coli

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pimlapas Leekitcharoenphon ◽  
Markus Hans Kristofer Johansson ◽  
Patrick Munk ◽  
Burkhard Malorny ◽  
Magdalena Skarżyńska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Mobile Genetic Elements ◽  
Whole Genome ◽  
Sequencing Analysis ◽  
Metagenomic Sequencing ◽  
Fecal Samples ◽  
E Coli ◽  
Genetic Elements

AbstractThe emergence of antimicrobial resistance (AMR) is one of the biggest health threats globally. In addition, the use of antimicrobial drugs in humans and livestock is considered an important driver of antimicrobial resistance. The commensal microbiota, and especially the intestinal microbiota, has been shown to have an important role in the emergence of AMR. Mobile genetic elements (MGEs) also play a central role in facilitating the acquisition and spread of AMR genes. We isolated Escherichia coli (n = 627) from fecal samples in respectively 25 poultry, 28 swine, and 15 veal calf herds from 6 European countries to investigate the phylogeny of E. coli at country, animal host and farm levels. Furthermore, we examine the evolution of AMR in E. coli genomes including an association with virulence genes, plasmids and MGEs. We compared the abundance metrics retrieved from metagenomic sequencing and whole genome sequenced of E. coli isolates from the same fecal samples and farms. The E. coli isolates in this study indicated no clonality or clustering based on country of origin and genetic markers; AMR, and MGEs. Nonetheless, mobile genetic elements play a role in the acquisition of AMR and virulence genes. Additionally, an abundance of AMR was agreeable between metagenomic and whole genome sequencing analysis for several AMR classes in poultry fecal samples suggesting that metagenomics could be used as an indicator for surveillance of AMR in E. coli isolates and vice versa.

Multiplex Quantitative PCR Assays for the Detection and Quantification of the Six Major Non-O157 Escherichia coli Serogroups in Cattle Feces†

2016 ◽  
Vol 79 (1) ◽  
pp. 66-74 ◽  
Author(s):  
P. B. SHRIDHAR ◽  
L. W. NOLL ◽  
X. SHI ◽  
B. AN ◽  
N. CERNICCHIARO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quantitative Pcr ◽  
Foodborne Pathogens ◽  
Virulence Genes ◽  
Target Genes ◽  
Culture Methods ◽  
Fecal Samples ◽  
E Coli ◽  
Cattle Feces ◽  
Before And After

ABSTRACT Shiga toxin–producing Escherichia coli (STEC) serogroups O26, O45, O103, O111, O121, and O145, called non-O157 STEC, are important foodborne pathogens. Cattle, a major reservoir, harbor the organisms in the hindgut and shed them in the feces. Although limited data exist on fecal shedding, concentrations of non-O157 STEC in feces have not been reported. The objectives of our study were (i) to develop and validate two multiplex quantitative PCR (mqPCR) assays, targeting O-antigen genes of O26, O103, and O111 (mqPCR-1) and O45, O121, and O145 (mqPCR-2); (ii) to utilize the two assays, together with a previously developed four-plex qPCR assay (mqPCR-3) targeting the O157 antigen and three virulence genes (stx1, stx2, and eae), to quantify seven serogroups and three virulence genes in cattle feces; and (iii) to compare the three mqPCR assays to a 10-plex conventional PCR (cPCR) targeting seven serogroups and three virulence genes and culture methods to detect seven E. coli serogroups in cattle feces. The two mqPCR assays (1 and 2) were shown to be specific to the target genes, and the detection limits were 4 and 2 log CFU/g of pure culture–spiked fecal samples, before and after enrichment, respectively. A total of 576 fecal samples collected from a feedlot were enriched in E. coli broth and were subjected to quantification (before enrichment) and detection (after enrichment). Of the 576 fecal samples subjected, before enrichment, to three mqPCR assays for quantification, 175 (30.4%) were quantifiable (≥4 log CFU/g) for at least one of the seven serogroups, with O157 being the most common serogroup. The three mqPCR assays detected higher proportions of postenriched fecal samples (P &lt; 0.01) as positive for one or more serogroups compared with cPCR and culture methods. This is the first study to assess the applicability of qPCR assays to detect and quantify six non-O157 serogroups in cattle feces and to generate data on fecal concentration of the six serogroups.

Long-Haul Transport and Lack of Preconditioning Increases Fecal Shedding of Escherichia coli and Escherichia coli O157:H7 by Calves†

2004 ◽  
Vol 67 (4) ◽  
pp. 672-678 ◽  
Author(s):  
S. J. BACH ◽  
T. A. McALLISTER ◽  
G. J. MEARS ◽  
K. S. SCHWARTZKOPF-GENSWEIN
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Fecal Samples ◽  
Fecal Shedding ◽  
E Coli ◽  
Susceptibility To Infection

The effects of weaning and transport on fecal shedding of Escherichia coli and on E. coli O157:H7 were investigated using 80 Angus and 94 Charolais range steer calves blocked by breed and assigned to four treatments. The calves were or were not preconditioned before transport on commercial cattle liner to the feedlot via long (15 h) or short (3 h) hauling duration, yielding preconditioned long haul (P-L; n = 44), preconditioned short haul (P-S; n = 44), nonpreconditioned long haul (NP-L; n = 43), and nonpreconditioned short haul (NP-S; n = 43). Preconditioned calves were vaccinated and weaned 29 and 13 days, respectively, before transport. Nonpreconditioned calves were weaned 1 day before long or short hauling, penned for 24 h and hauled again for 2 h, and vaccinated on arrival at the feedlot. Fecal samples were collected from calves while on pasture, at weaning, at loading for transport, on arrival at the feedlot, twice in the first week, and on days 7, 14, 21, and 28 for enumeration of total E. coli (biotype 1) and detection of E. coli O157:H7. No calves were positive for E. coli O157:H7 before transport. Following transport, more (P &lt; 0.005) NP-L calves (6 of 43) tested positive for E. coli O157:H7 than did P-L (1 of 44), NP-S (1 of 43), or P-S (0 of 44) calves, and on days 0, 1, 7, and 21, their levels of shedding of E. coli were higher (P &lt; 0.005). The calves' susceptibility to infection from the environment (possibly the holding facilities or feedlot pens) was likely elevated by the stresses of weaning, transport, and relocation. Lack of preconditioning and long periods of transport (NP-L) increased fecal shedding of E. coli and E. coli O157:H7. Preconditioning may serve to reduce E. coli O157:H7 shedding by range calves on arrival at the feedlot.

scholarly journals Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

2016 ◽  
Vol 14 (1) ◽  
pp. 63-68 ◽  
Author(s):  
MM Akter ◽  
S Majumder ◽  
KH MNH Nazir ◽  
M Rahman
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Molecular Detection ◽  
Chain Reaction ◽  
Specific Primers ◽  
Fecal Samples ◽  
E Coli ◽  
Uremic Syndrome ◽  
Polymerase Chain ◽  
Positive Isolate

Shiga toxin-producing Escherichia coli (STEC) are zoonotically important pathogen which causes hemorrhagic colitis, diarrhea, and hemolytic uremic syndrome in animals and humans. The present study was designed to isolate and identify the STEC from fecal samples of diarrheic cattle. A total of 35 diarrheic fecal samples were collected from Bangladesh Agricultural University (BAU) Veterinary Teaching Hospital. The samples were primarily examined for the detection of E. coli by cultural, morphological and biochemical characteristics, followed by confirmation of the isolates by Polymerase Chain Reaction (PCR) using gene specific primers. Later, the STEC were identified among the isolated E. coli through detection of Stx-1 and Stx-2 genes using duplex PCR. Out of 35 samples, 25 (71.43%) isolates were confirmed to be associated with E. coli, of which only 7 (28%) isolates were shiga toxin producers, and all of them were positive for Stx-1. However, no Stx-2 positive isolate could be detected. From this study, it may be concluded that cattle can act as a reservoir of STEC which may transmit to human or other animals.J. Bangladesh Agril. Univ. 14(1): 63-68, June 2016

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