scholarly journals An Intramuscular Multivalent Vaccine Against Pathogenic Escherichia Coli Injected in Suckling Piglets Reduced the Prevalence of Pathogenic E. Coli and Lawsonia Intracellularis Until Slaughter

2021 ◽  
Author(s):  
Lívia Mendonça Pascoal ◽  
Sarah Rodrigues Chagas ◽  
Francisco J. Pallarés ◽  
Juan J. Quereda ◽  
Juan Manuel Herrero Medrano ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Intestinal Mucosa ◽  
Animal Health ◽  
Control Group ◽  
Lawsonia Intracellularis ◽  
Colon Tissue ◽  
Fecal Samples ◽  
E Coli ◽  
Suckling Piglets

Abstract Background: The present study aims to evaluate the efficacy of an intramuscular multivalent Escherichia coli vaccine for suckling piglets against infection not only by pathogenic E. coli but also by pathogens involved in Porcine Enteric Disease Complex (PEDC). Vaccinated Group had piglets vaccinated at days 10 and 20 of life with Colidex-C® (Vetia Animal Health, Spain), and Control Group had piglets that received sterile saline solution injection at the same days of life. We collected fecal samples in the farm from animals presenting diarrhea and intestinal mucosa swabs and ileum and colon tissue at slaughter and then performed PCR to identify E. coli virulence factors genes. Furthermore, we performed PCR to identify Lawsonia intracellularis, Brachyspira hyodisenteriae, and Salmonella spp.Results: Regarding fecal samples, 0% from Vaccinated Group was positive for E. coli, while Control Group had 94.1% of positive samples (p<0.0001). With respect to intestinal mucosa swab, 0% of the samples from Vaccinated Group were positive for E. coli, while 100% from Control Group were positive (p=0.001). Regarding ileum and colon tissue samples, 35% were positive for E. coli in Vaccinated Group and 85% in Control Group (p=0.001); Gcnt had a higher frequency of F41 (p=0.018), LT (p=0.018) and Sta (p=0.028) virulence factors genes. No sample was positive for Salmonella spp. nor for B. hyodisenteriae, but there were positive samples L. intracellularis; real-time PCR was performed and the frequencies found were 40% and 20% of ileum and colon positive samples in Vaccinated Group and 100% for ileum and 70% for colon in Control Group (p<0.001 for ileum and p=0.001 for colon).Conclusion: The results indicate that the E. coli vaccine for piglets may be a strategy to control E. coli infection. E. coli vaccines emerge as a probable strategy to help control L. intracellularis and, maybe, other enteric pathogens of pigs not evaluated in this study.

scholarly journals Multiple acquisitions of CTX-M plasmids in the rare D2 genotype of Escherichia coli provide evidence for convergent evolution

Microbiology ◽  
2009 ◽  
Vol 155 (5) ◽  
pp. 1656-1668 ◽  
Author(s):  
Catherine Deschamps ◽  
Olivier Clermont ◽  
Marie Claire Hipeaux ◽  
Guillaume Arlet ◽  
Erick Denamur ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Convergent Evolution ◽  
Fine Tuning ◽  
Control Group ◽  
M Gene ◽  
Major Health Problem ◽  
Major Health ◽  
E Coli ◽  
The Relationship

Over the last decade, CTX-M enzymes have become the most prevalent extended-spectrum β-lactamases (ESBLs) worldwide, mostly in Escherichia coli, causing a major health problem. An epidemiological relationship has been established between a rare genotype of E. coli, the D2 genotype, and the presence of CTX-M genes. We investigated this striking association by exploring the genetic backgrounds of 18 D2 genotype CTX-M-producing strains and of the plasmids encoding CTX-M enzymes. The 18 strains had different genetic backgrounds, as assessed by multilocus sequence and O typing, and were associated with various plasmids bearing diverse CTX-M genes. The region encompassing the genetic marker of the D2 genotype (TSPE4.C2) was not correlated with the presence of CTX-M genes. CTX-M-producing D2 strains had far fewer virulence factors than a control group of 8 non-ESBL-producing D2 strains, and an inverse relationship was found between the number of co-resistances associated with the CTX-M gene and the number of virulence factors found in the strain. These findings provide evidence for multiple acquisitions of plasmids carrying CTX-M genes in different D2 genotype strains. They strongly suggest that convergent evolution has occurred, and indicate that there has been selection for the association of a specific genetic background of the strain and the CTX-M gene. This fine-tuning of the relationship between the D2 genotype and CTX-M genes presumably increases the fitness of the strain, indicating a role for the host cell in the acquisition and dissemination of CTX-M genes.

scholarly journals Effect of Protracted Free-Choice Chlortetracycline-Medicated Mineral for Anaplasmosis Control on Escherichia coli Chlortetracycline Resistance Profile from Pastured Beef Cattle

2021 ◽  
Vol 9 (12) ◽  
pp. 2495
Author(s):  
Alyssa R. Toillion ◽  
Emily J. Reppert ◽  
Raghavendra G. Amachawadi ◽  
K. C. Olson ◽  
Johann F. Coetzee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Treatment Period ◽  
Free Choice ◽  
Current Control ◽  
Control Group ◽  
Fecal Samples ◽  
E Coli ◽  
Control Options ◽  
Cattle Herds

Anaplasmosis is an economically-significant, hemolytic, tick-borne disease of cattle caused by Anaplasma marginale which can cause clinical anemia and death. Current control options are limited, and FDA-approved antimicrobial control options do not have a defined duration of use. A practical and routinely used anaplasmosis control method involves feeding free-choice chlortetracycline (CTC)-medicated mineral to pastured cattle for several months. Constant antimicrobial use poses the risk of expediting the development and dissemination of antimicrobial resistance in off-target commensal bacteria in the bovine gastrointestinal tract. The objective of this study was to determine the CTC-susceptibility of Escherichia coli isolated from anaplasmosis endemic beef cattle herds provided different FDA-approved free-choice CTC-medicated mineral formulations, all intended to provide cattle a dosage of 0.5 to 2.0 mg CTC/lb bodyweight per day. A closed-herd, comprised of Hereford-Angus cows, naturally endemic for anaplasmosis, were grazed in five different pastures with one herd serving as an untreated control group. The other cattle herds were randomly assigned one of four FDA-approved CTC-medicated mineral formulations (700, 5000, 6000, and 8000 g CTC/ton) labeled for “the control of active anaplasmosis” and provided their respective CTC-medicated mineral formulation for five consecutive months. Fecal samples were collected monthly from a subset of cows (n = 6 or 10) per pasture. Fecal samples were cultured for E. coli isolates and the minimal inhibitory concentration of CTC was determined. Baseline CTC-susceptibility of E. coli was variable among all treatment and control groups. The susceptibility of E. coli isolates was significantly different between study herds over the treatment period (p = 0.0037 across time and 0.009 at the final sampling time). The interaction between study herds and treatment period was not significant (p = 0.075).

scholarly journals Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh

2019 ◽  
Vol 12 (10) ◽  
pp. 1578-1583 ◽  
Author(s):  
Md Samun Sarker ◽  
Abdul Ahad ◽  
Saurav Kumar Ghosh ◽  
Md Shahriar Mannan ◽  
Arup Sen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Water Samples ◽  
Animal Health ◽  
Significant Proportion ◽  
Water Sources ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Fecal Samples ◽  
E Coli

Background and Aim: The emergence and rapid dissemination of multidrug-resistant (MDR) bacteria in different ecosystems is a growing concern to human health, animal health, and the environment in recent years. The study aimed to determine the antibiotic resistance in Escherichia coli from deer and nearby water sources at two different Safari parks in Bangladesh. Materials and Methods: A number of 55 fresh fecal samples of deer and six water samples from nearby lakes were collected from two Safari parks. Samples were processed, cultured, and carried out biochemical tests for E. coli. The antibiotic susceptibility was determined by disk diffusion method. To identify the resistance genes, polymerase chain reaction was performed. Results: A total of 32 E. coli isolates from 55 fecal samples and 6 of 6 E. coli isolates from lake water were isolated. From fecal E. coli isolates, ampicillin and sulfamethoxazole were 90.63% (n=29/32) resistant and 87.5% (n=28/32) were resistant to tetracycline and nalidixic acid. High resistance was also observed to other antibiotics. On the contrary, all E. coli isolates from water sources were 100% (n=6/6) resistant to ampicillin, tetracycline, sulfamethoxazole, and nalidixic acid. MDR was revealed in all water samples, whereas 96.88% (n=31/32) was found in fecal isolates. A number of blaTEM, tetA, and Sul2 genes were detected from both isolates. Conclusion: This study for the 1st time highlights, a significant proportion of E. coli isolates in wildlife deer and nearby water sources were MDR in Bangladesh.

scholarly journals Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups

2011 ◽  
Vol 26 (2) ◽  
pp. 144-148
Author(s):  
Eliana Maria Ferreira Gouveia ◽  
Iandara Schettert Silva ◽  
Gerson Nakazato ◽  
Flábio Ribeiro de Araujo ◽  
Marilene Rodrigues Chang
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Experimental Infection ◽  
Control Group ◽  
Enteropathogenic Escherichia Coli ◽  
Enteric Coating ◽  
Coating Solution ◽  
E Coli ◽  
Polymerase Chain ◽  
Enteric Coated

PURPOSE: To verify the possibility of an experimental infection with enteropathogenic Escherichia coli and to confirm by PCR that the symptoms manifested after infection were due to the virulence factors of the studied bacteria. METHODS: Experimental units were 14 healthy pups of Boxer breed, aged 60 days. The animals were divided into three groups. One animal from each litter was included in a control group and the remaining animals were divided into two groups: one inoculated with strain 4083, and another one inoculated with strain SPA14. Gelatinous capsules coated with enteric-coating solution were used for the inoculation of strains. E. coli isolation from feces was performed for all tested animals, and the extracted DNA was subjected to Polymerase Chain Reaction (PCR). RESULTS: All infected animals presented diarrhea and had the gene eae amplified by PCR. CONCLUSION: The efficiency of PCR for the studied strains indicates that this technique can be recommended for the diagnosis of enteropathogenic Escherichia coli as a differential from other pathogens causing diarrhea. It may also be used in the future to verify whether other virulence factors (bfpA gene and EAF plasmid) persist after infection and to assess the pathogenicity of these bacteria.

scholarly journals Effects of Flavophospholipol on Resistance in Fecal Escherichia coli and Enterococci of Fattening P

2002 ◽  
Vol 46 (1) ◽  
pp. 110-118 ◽  
Author(s):  
A. E. van den Bogaard ◽  
M. Hazen ◽  
M. Hoyer ◽  
P. Oostenbach ◽  
E. E. Stobberingh
Keyword(s):  
Escherichia Coli ◽  
Intestinal Flora ◽  
Cross Resistance ◽  
Control Group ◽  
Growth Promoter ◽  
Experimental Conditions ◽  
Fecal Samples ◽  
E Coli ◽  
Vancomycin Resistant ◽  
Group B

ABSTRACT A “plasmid-curing effect” of multiresistant Escherichia coli by flavophospholipol, an antibiotic used as an antimicrobial growth promoter (AMGP) in animal feeds, has been reported to occur in vitro and in vivo under experimental conditions. In this study, the effect of flavophospholipol under field conditions was studied. The prevalence and degree (proportion of resistant strains to the total numbers present per gram of feces) of resistance of indicator bacteria, E. coli and enterococci, was determined in fecal samples from three groups of pigs that were fed a commercial finisher feed without any AMGP. Group A was the negative control group without any AMGP, group B received the same feed with 9 mg of flavophospholipol/kg of feed (study group), and group C received the same feed with 15 mg of avoparcin/kg (positive control). Fecal samples from each pig were collected at the start and at the end of the study and assessed for the prevalence and degree of resistance against antibiotics commonly used either for therapy in pig medicine or as an AMGP. Before the start of the study, all pigs were colonized with multiresistant E. coli by mixing three resistant pig isolates through their feed after disturbance of the colonization resistance of the intestinal flora by a 3-day course of lincomycin and spectinomycin. At the end of the study, the overall prevalence and degree of resistance of E. coli in the fecal flora had increased significantly in groups A and C but remained at the same level as at the start of the study in group B. The prevalence of vancomycin resistance was 44 and 41% in groups A and B, respectively, but only very low numbers of vancomycin-resistant enterococci (VRE) per gram of feces were found. In the avoparcin-fed group, the prevalence was 72%, and in 57% of the samples, more than 50% of all enterococci present were vancomycin resistant. The prevalence of resistant Enterococcus faecalis increased only in the flavophospholipol-exposed group, from 23% before the start of the study to 43% at the end of the study. It was concluded that flavophospholipol effectively suppressed the augmentation and dissemination of multiresistant E. coli in the intestinal flora of fattening pigs. Avoparcin use strongly selected for VRE carriage and excretion. Therefore, as neither flavophospholipol nor any related molecule is used therapeutically, no cross-resistance with therapeutic antibiotics exists and no transmissible resistance has been shown; the major decrease in resistance in intestinal E. coli of flavophospholipol-fed animals seemed to outweigh the small increase in the risk of transfer of flavophospholipol-resistant E. faecalis from animals to humans via the food chain.

scholarly journals Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia

2020 ◽  
Vol 76 (01) ◽  
pp. 6326-2020
Author(s):  
JADRANKA ŽUTIĆ ◽  
OLIVERA VALČIĆ ◽  
VESNA MILIĆEVIĆ ◽  
LJUBIŠA VELJOVIĆ ◽  
JASNA KURELJUŠIĆ ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Enterotoxigenic Escherichia Coli ◽  
Gene Encoding ◽  
E Coli ◽  
Genes Encoding ◽  
Virulence Pattern ◽  
Suckling Piglets ◽  
Asta Gene

A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.

scholarly journals Identification of Virulence Factors of Escherichia coli Isolates from Fecal Samples of Calves in Southern Brazil

2017 ◽  
Vol 45 (1) ◽  
pp. 6
Author(s):  
Sergio Farias Vargas Júnior ◽  
Rodrigo Casquero Cunha ◽  
Daniela Isabel Brayer Pereira ◽  
Sônia De Ávila Botton ◽  
Silvia Regina Leal Ladeira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Virulence Factor ◽  
Rio Grande ◽  
Southern Region ◽  
Susceptibility Test ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Fecal Samples ◽  
E Coli

Background: Escherichia coli (E. coli) is an enteropathogen that commonly causes diarrhea in calves. However, not all E. coli isolates are pathogenic. The aim of this study was to identify E. coli virulence factors derived from fecal samples collected in the southern region of Rio Grande do Sul state (RS) from calves with and without diarrhea, as well as investigate the antimicrobial susceptibility of E. coli isolates from calves with diarrhea.Materials, Methods & Results: Forty stool samples were collected in 12 farms, each one from calves having one day to six months of age, with and without diarrhea. The total DNA of from these isolates was extracted and a PCR using primers specific for the virulence factors Stx1, Eae, F41, F5 and STa was conducted. The susceptibility testing used the disk diffusion method and the susceptibility profile was evaluated against the following antimicrobials: ampicillin, penicillin, chloramphenicol, enrofloxacin, gentamicin, trimethoprim, sulfonamide, tetracycline and streptomycin. From all calves, 15 (15/40, 37.5%) had diarrheal stools and 25 (25/40, 62.5%) had normal or semi-liquid stools. Twelve (12/40; 30%) E. coli isolates showed at least one virulence factor. These factors were found in four isolates (4/15; 26.6%) from diarrheal stools and eight isolates (8/25; 28.5%) from normal stool. The Stx1 factor was identified in five isolates (5/40; 12.5%), and the Eae and the Sta factors in one (1/40; 0.2%) and in atypical associations between Stx1 and Eae and also between Eae and F41 in two isolates (2/40; 0.5%). Also, the Eae and Sta factors were identified in one isolate (1/40; 0.2%). The susceptibility test showed resistance to penicillin and tetracycline in 93% and 80% of the tested isolates, respectively.Discussion: The identification of virulence factors is necessary because E. coli is an enterobacterium present in calves gastrointestinal tract, to prove its pathogenicity. The virulence factor most commonly found in E. coli isolates derived from feces of calves with and without diarrhea in the southern region of the RS was the Stx1 (Shiga toxin-producing E. coli STEC). It is likely that the highest occurrence of E. coli isolates positive for the Stx1 virulence factor was due to the fact that cattle were the main reservoirs of this type of bacteria. The occurrence of enterohemorrhagic E. coli (EHEC) in animals of nine and 34 days of life, respectively, is highlighted. Studies have shown that contamination of animal foods with EHEC can cause enteric disorders, hemorrhagic colitis, and uremic hemolytic syndrome (UHS) in humans. Although in the present study the identification of the Stx2 factor was not performed, authors describe that the presence of the genes encoding Stx2 and Eae is determinant in the occurrence of UHS. In the susceptibility test, it was observed that E. coli isolates from diarrheal stools showed resistance to antimicrobials penicillin (10 mg) and tetracycline (30 mg) [93% and 80%, respectively], ampicillin (10 mg) [47%], streptomycin (10 mg) [47%], trimethoprim (5 mg) [47%] and sulfonamide (300 mg) [53%]. Although the percentage of antimicrobial resistance varies among studies, it is believed that the indiscriminate use of antimicrobial therapies as a common practice among rural properties contributes to bacterial resistance to these drugs. The sensitivity profile to antimicrobials showed that the analyzed Escherichia coli isolates are resistant to the antimicrobials commonly used for diarrhea treatment in the southern region of the Rio Grande do Sul state, Southern Brazil.

scholarly journals Effects of In-Feed Chlortetracycline Prophylaxis in Beef Cattle on Animal Health and Antimicrobial-Resistant Escherichia coli

2016 ◽  
Vol 82 (24) ◽  
pp. 7197-7204 ◽  
Author(s):  
Getahun E. Agga ◽  
John W. Schmidt ◽  
Terrance M. Arthur
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Human Health ◽  
Bacterial Infections ◽  
Animal Health ◽  
Agricultural Practices ◽  
Control Group ◽  
Content Type ◽  
E Coli ◽  
The Impact

ABSTRACTConcerns have been raised that in-feed chlortetracycline (CTC) may increase antimicrobial resistance (AMR), specifically tetracycline-resistant (TETr)Escherichia coliand third-generation cephalosporin-resistant (3GCr)E. coli. We evaluated the impact of a 5-day in-feed CTC prophylaxis on animal health, TETrE. coli, and 3GCrE. coli. A control group of cattle (n= 150) received no CTC, while a CTC group (n= 150) received in-feed CTC (10 mg/lb of body weight/day) from the 5th to the 9th day after feedlot arrival. Over 25% (38/150) of the animals in the control group developed illnesses requiring therapeutic treatment with antimicrobials critically important to human medicine. Only two animals (1.3%) in the CTC group required such treatments. Fecal swab and pen surface occurrences of genericE. coli(isolated on media that did not contain antimicrobials of interest and were not isolated based on any specific resistance), TETrE. coli, and 3GCrE. coliwere determined on five sampling occasions: arrival at the feedlot, 5 days posttreatment (5 dpt), 27 dpt, 75 dpt, and 117 dpt. On 5 dpt, TETrE. coliconcentrations were higher for the CTC group than the control group (P< 0.01). On 27 dpt, 75 dpt, and 117 dpt, TETrE. coliconcentrations did not differ between groups. 3GCrE. colioccurrences did not differ between control and CTC groups on any sampling occasion. For both groups, generic, TETr, and 3GCrE. colioccurrences were highest on 75 dpt and 117 dpt, suggesting that factors other than in-feed CTC contributed more significantly to antimicrobial-resistantE. colioccurrence.IMPORTANCEThe occurrence of human bacterial infections resistant to antimicrobial therapy has been increasing. It has been postulated that antimicrobial resistance was inevitable, but the life span of the antimicrobial era has been prematurely compromised due to the misuse of antimicrobials in clinical and agricultural practices. Direct evidence relating the use of antimicrobials in livestock production to diminished human health outcomes due to antimicrobial resistance is lacking, and the U.S. Food and Drug Administration has taken an approach to maximize therapeutic efficacy and minimize the selection of resistant microorganisms through judicious use of antimicrobials. This study demonstrated that prophylactic in-feed treatment of chlortetracycline administered for 5 days to calves entering feedlots is judicious, as this therapy reduced animal morbidity, reduced the use of antimicrobials more critical to human health, and had no long-term impact on the occurrence of antimicrobial-resistantE. coli.

scholarly journals Virulence Genes Profiles and Phylogenetic Origin of Escherichia coli from Acute and Chronic Intestinal Diseases Revealed by Comparative Genomic Hybridization Microarray

2012 ◽  
Vol 61 (2) ◽  
pp. 105-110
Author(s):  
BEATA SOBIESZCZAŃSKA ◽  
URSZULA KASPRZYKOWSKA ◽  
MICHAŁ TURNIAK ◽  
HENRYK MACIEJEWSKI ◽  
ROMAN FRANICZEK ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Virulence Factors ◽  
Acute Diarrhea ◽  
Virulence Genes ◽  
Comparative Genomic ◽  
Control Group ◽  
Intestinal Diseases ◽  
E Coli

The association between Escherichia coli virulence factors and chronic intestinal disorders is mostly unknown. The presented study compared the distribution of virulence genes and phylogroups among E. coli isolated from chronic intestinal disorders such as Crohn's disease and irritable bowel syndrome (IBS) with strains isolated from patients with acute diarrhea as a control group. The presence of 159 virulence genes corresponding to known E. coli pathotypes was determined among 78 E. coli archive strains isolated from IBS, acute diarrhea and Crohn's disease using CGH microarray. E. coli isolated from IBS demonstrated a mosaic of virulence genes specific to enteropathogenic, enterotoxigenic, enterohemorrhagic E. coli strains and Shigella species. In contrast, virulence factors and phylogroups distribution among E. coli isolated from children with acute diarrhea was similar to extraintestinal E. coli strains that probably acquired some virulence genes. The acquisition of virulence genes might have an impact on diarrheagenic potential of these strains. On the other hand, E. coli isolated from children with Crohn's disease seem to be similar to adherent-invasive E. coli strains (AIEC), as it lack most known virulence genes. The presented study showed that these analyzed groups of E. coli strains differed from each other with the respect to the distribution of virulence genes. The differences in gene content support the idea that the participation of E. coli in chronic intestinal diseases is mostly related to virulence potential of these strains.

scholarly journals Antimicrobial resistance pattern of Escherichia coli strains isolated from poultry farmers and poultry slaughterers in Morocco

10.3823/820 ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
CHAIBA Abdellah ◽  
Rhazi Filali Fouzia
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Antimicrobial Agents ◽  
Resistance Pattern ◽  
Control Group ◽  
Fecal Samples ◽  
E Coli ◽  
Poultry Workers ◽  
Poultry Farmers

Aim: The objective of this study is to characterize the prevalence of antibiotic resistance in Escherichia coli isolates from the fecal samples of poultry workers, and to study the possible dissemination of resistant E. coli  from  poultry to humans. Methodology: Sixty four E. coli strains isolated from the fecal samples of poultry workers and 35 isolates from a control group workers were tested for antibiotic resistance by agar disk diffusion with 11 antimicrobial agents. Results: Resistance of E. coli isolated from poultry workers to tetracycline, ampicillin and norfloxacin were significantly (p < 0,05) higher than those isolated from the control group. All E. coli isolates were susceptible to cefotaxime, and most of them are susceptible to gentamycin, amikacin, cefoxitin and ertapenem. Multidrug resistance is alarmingly high in all groups, but was highest in poultry farmers isolates (84%) and poultry slaughterers isolates (80%). Approximately 25 % of the isolates of poultry workers showed resistance to four or more antibiotics. Conclusion: This study implies  that occupational exposure to antimicrobial-resistant E. coli from animal contact in the broiler chicken industry may be an important route of entry for antimicrobial-resistant E. coli into the community. Keywords : Escherichia coli ; Poultry Workers ; Antibiotic Resistance ; Multidrug Resistance ; Morocco.

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