Development and Validation of 697 Novel Polymorphic Genomic and EST-SSR Markers in the American Cranberry (Vaccinium macrocarpon Ait.)

The flavonoids of american cranberry (Vaccinium macrocarpon Ait.) are documented to be beneficial for human health. Among their benefits is a high antioxidant potential, with anthocyanin glycosides being the main contributors. Flavonoid glucose conjugates are reported to be more bioavailable than those with other sugar conjugates. The anthocyanin glycosides of V. macrocarpon fruit are mainly galactosides and arabinosides of the aglycones, cyanidin and peonidin, with less than 8% glucosides. In contrast, the fruit anthocyanins of another cranberry species, V. oxycoccus L. were found to be largely glucosides of cyanidin and peonidin. Interspecific hybrids between these two species were intermediate to the parental species in the proportion of fruit anthocyanin glucosides. About half the progeny (1:1 segregation) in a backcross population (to V. macrocarpon) maintained the relatively high anthocyanin glucoside ratio. In this study, we demonstrate the genetic manipulation of anthocyanin glycosylation in cranberry using interspecific hybridization, resulting in dramatically increased glucose-conjugated anthocyanins.

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Seed lipids of the American cranberry (vaccinium macrocarpon)

Phytochemistry ◽

10.1016/s0031-9422(00)88185-6 ◽

1969 ◽

Vol 8 (11) ◽

pp. 2219-2222 ◽

Cited By ~ 9

Author(s):

Rodney Croteau ◽

Irving S. Fagerson

Keyword(s):

Vaccinium Macrocarpon ◽

Seed Lipids ◽

American Cranberry

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Antibacterial activities of a polyphenolic-rich extract prepared from American cranberry (Vaccinium macrocarpon) fruit pomace against Listeria spp.

LWT ◽

10.1016/j.lwt.2020.109056 ◽

2020 ◽

Vol 123 ◽

pp. 109056 ◽

Cited By ~ 4

Author(s):

Moussa S. Diarra ◽

Yousef I. Hassan ◽

Glenn S. Block ◽

John C.G. Drover ◽

Pascal Delaquis ◽

...

Keyword(s):

Antibacterial Activities ◽

Vaccinium Macrocarpon ◽

American Cranberry ◽

Fruit Pomace

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Variability in the Qualitative and Quantitative Composition and Content of Phenolic Compounds in the Fruit of Introduced American Cranberry (Vaccinium macrocarpon Aiton)

Plants ◽

10.3390/plants9101379 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1379

Author(s):

Ieva Gudžinskaitė ◽

Elicija Stackevičienė ◽

Mindaugas Liaudanskas ◽

Kristina Zymonė ◽

Vaidotas Žvikas ◽

...

Keyword(s):

Phenolic Compounds ◽

Vaccinium Macrocarpon ◽

Quantitative Composition ◽

Qualitative And Quantitative ◽

American Cranberry ◽

Fruit Samples ◽

Phytochemical Profile ◽

Ethanol Extracts ◽

The Individual

The aim of this study was to determine the composition and content of phenolic compounds in ethanol extracts of eight different cultivars of American cranberry (Vaccinium macrocarpon Aiton) fruit using spectrophotometric and UPLC-ESI-MS/MS analysis and to evaluate the antioxidant activity in vitro of these extracts. The highest total amount of phenolic compounds evaluated via Folin–Ciocalteu spectrophotometry was detected in American cranberry fruit samples of the ‘Bain’ clone, and the highest total amount of flavonoids was found in samples of the ‘Drever’ and ‘Baiwfay’ cultivars. The highest total amount of the individual phenolic compounds (519.53 ± 25.12 mg/g DW) identified and quantitatively evaluated via chromatography was detected in samples of the ‘Searles’ cranberry cultivar. In the studied cranberry samples, the predominant phenolic compounds were hyperoside, quercetin, and procyanidin A2, while the amounts of other compounds were significantly lower. HCA and PCA revealed that ‘Woolman’, ‘Holliston’, ‘Pilgrim, and ‘Searles’ fruit samples had different quantitative content of phenolic compounds from other cranberry cultivars. Meanwhile, fruit of ‘Baiwfay’, ‘Drever’, ‘Bain’, and ‘Bergman’ were similar in their phytochemical profile.

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Genome-Wide Novel Genic Microsatellite Marker Resource Development and Validation for Genetic Diversity and Population Structure Analysis of Banana

Genes ◽

10.3390/genes11121479 ◽

2020 ◽

Vol 11 (12) ◽

pp. 1479

Author(s):

Manosh Kumar Biswas ◽

Mita Bagchi ◽

Dhiman Biswas ◽

Jennifer Ann Harikrishna ◽

Yuxuan Liu ◽

...

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Comparative Mapping ◽

In Silico ◽

Large Scale ◽

Crop Improvement ◽

Musa Spp ◽

Population Structure Analysis ◽

A Genome ◽

Development And Validation

Trait tagging through molecular markers is an important molecular breeding tool for crop improvement. SSR markers encoded by functionally relevant parts of a genome are well suited for this task because they may be directly related to traits. However, a limited number of these markers are known for Musa spp. Here, we report 35136 novel functionally relevant SSR markers (FRSMs). Among these, 17,561, 15,373 and 16,286 FRSMs were mapped in-silico to the genomes of Musa acuminata, M. balbisiana and M. schizocarpa, respectively. A set of 273 markers was validated using eight accessions of Musa spp., from which 259 markers (95%) produced a PCR product of the expected size and 203 (74%) were polymorphic. In-silico comparative mapping of FRSMs onto Musa and related species indicated sequence-based orthology and synteny relationships among the chromosomes of Musa and other plant species. Fifteen FRSMs were used to estimate the phylogenetic relationships among 50 banana accessions, and the results revealed that all banana accessions group into two major clusters according to their genomic background. Here, we report the first large-scale development and characterization of functionally relevant Musa SSR markers. We demonstrate their utility for germplasm characterization, genetic diversity studies, and comparative mapping in Musa spp. and other monocot species. The sequences for these novel markers are freely available via a searchable web interface called Musa Marker Database.

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