scholarly journals Timbe (Acaciella angustissima) Pods Extracts Reduce the Levels of Glucose, Insulin and Improved Physiological Parameters, Hypolipidemic Effect, Oxidative Stress and Renal Damage in Streptozotocin-Induced Diabetic Rats

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2812 ◽  
Author(s):  
Adriana Rodríguez-Méndez ◽  
Wendy Carmen-Sandoval ◽  
Consuelo Lomas-Soria ◽  
Ramón Guevara-González ◽  
Rosalía Reynoso-Camacho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Damage ◽  
Methanol Extract ◽  
Serum Insulin ◽  
Diabetic Rats ◽  
Physiological Parameters ◽  
Hypolipidemic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Uptake Activity

In Mexico one in 14 deaths are caused by diabetes mellitus (DM) or by the macro and microvascular disorders derived from it. A continuous hyperglycemic state is characteristic of DM, resulting from a sustained state of insulin resistance and/or a dysfunction of β-pancreatic cells. Acaciella angustissima is a little studied species showing a significant antioxidant activity that can be used as treatment of this disease or preventive against the complications. The objective of this study was to explore the effect of oral administration of A. angustissima methanol extract on physiological parameters of streptozotocin-induced diabetic rats. The results indicated a significant reduction in blood glucose levels, an increase in serum insulin concentration, a decrease in lipid levels and an improvement in the parameters of kidney damage by applying a concentration of 100 mg/Kg B.W. However, glucose uptake activity was not observed in the adipocyte assay. Moreover, the extract of A. angustissima displayed potential for the complementary treatment of diabetes and its complications likely due to the presence of bioactive compounds such as protocatechuic acid. This study demonstrated that methanol extract of Acacciella angustissima has an antidiabetic effect by reducing the levels of glucose, insulin and improved physiological parameters, hypolipidemic effect, oxidative stress and renal damage in diabetic rats.

scholarly journals Effects of Apocynin on Heart Muscle Oxidative Stress of Rats with Experimental Diabetes: Implications for Mitochondria

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 335
Author(s):  
Estefanía Bravo-Sánchez ◽  
Donovan Peña-Montes ◽  
Sarai Sánchez-Duarte ◽  
Alfredo Saavedra-Molina ◽  
Elizabeth Sánchez-Duarte ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Sensitivity ◽  
Cardiac Tissue ◽  
Diabetic Rats ◽  
Oxidase Inhibitor ◽  
Beneficial Effects ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Transport Chain ◽  
Male Wistar Rats

Diabetes mellitus (DM) constitutes one of the public health problems today. It is characterized by hyperglycemia through a defect in the β-cells function and/or decreased insulin sensitivity. Apocynin has been tasted acting directly as an NADPH oxidase inhibitor and reactive oxygen species (ROS) scavenger, exhibiting beneficial effects against diabetic complications. Hence, the present study’s goal was to dissect the possible mechanisms by which apocynin could mediate its cardioprotective effect against DM-induced oxidative stress. Male Wistar rats were assigned into 4 groups: Control (C), control + apocynin (C+A), diabetes (D), diabetes + apocynin (D+A). DM was induced with streptozotocin. Apocynin treatment (3 mg/kg/day) was applied for 5 weeks. Treatment significantly decreased blood glucose levels and insulin resistance in diabetic rats. In cardiac tissue, ROS levels were higher, and catalase enzyme activity was reduced in the D group compared to the C group; the apocynin treatment significantly attenuated these responses. In heart mitochondria, Complexes I and II of the electron transport chain (ETC) were significantly enhanced in the D+A group. Total glutathione, the level of reduced glutathione (GSH) and the GSH/ oxidized glutathione (GSSG) ratio were increased in the D+A group. Superoxide dismutase (SOD) and the glutathione peroxidase (GSH-Px) activities were without change. Apocynin enhances glucose uptake and insulin sensitivity, preserving the antioxidant defense and mitochondrial function.

Treatment with N-acetylcysteine does not alter blood glucose levels and the oxidative stress status in diabetic rats

2013 ◽  
Vol 4 (1) ◽  
pp. 5 ◽  
Author(s):  
AndréValle de Bairros ◽  
Fernando de Freitas ◽  
Mirna Leal ◽  
Cinthia Mazzanti ◽  
AnaPaula Moreira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Oxidative Stress Status

scholarly journals Decrease of Klotho in the Kidney of Streptozotocin-Induced Diabetic Rats

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Meng-Fu Cheng ◽  
Li-Jen Chen ◽  
Juei-Tang Cheng
Keyword(s):  
Oxidative Stress ◽  
High Glucose ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Renal Functions ◽  
Blood Glucose Levels ◽  
Convoluted Tubules ◽  
The Brain ◽  
Darby Canine Kidney

Theklothogene is expressed in a limited number of tissues, most notably in distal convoluted tubules in the kidney and choroid plexus in the brain. A previous study suggested that Klotho increases resistance to oxidative stress. However, changes of Klotho expression in high glucose-induced oxidative stress remain unclear. In the present study, we used streptozotocin-induced diabetic rats (STZ rats) to examine the effects of insulin, phloridzin or antioxidant, tiron on diabetic nephropathy. Both insulin and phloridzin reversed the lower Klotho expression levels in kidneys of STZ rats by the correction of hyperglycemia. Also, renal functions were improved by these treatments. In addition to the improvement of renal functions, the decrease of Klotho expression in kidney of STZ rats was also reversed by tiron without changing blood glucose levels. The reduction of oxidative stress induced by high glucose can be considered for this action of tiron. This view was further confirmed in vitro using high glucose-exposed Madin-Darby canine kidney (MDCK) epithelial cells. Thus, we suggest that decrease of oxidative stress is not only responsible for the improvement of renal function but also for the recovery of Klotho expression in kidney of STZ rats.

scholarly journals Ameolirative effects of garlic (Allium sativum L.,) in streptozotocin induced oxidative stress in diabetic brain of rat

2020 ◽  
Vol 11 (4) ◽  
pp. 7950-7957
Author(s):  
Pratap Reddy K ◽  
Bhaskar Nagilla ◽  
Varija K
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Polyol Pathway ◽  
Diabetic Rats ◽  
Garlic Extract ◽  
Advanced Glycation ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Glycation End Products ◽  
End Products

The aim of the present study was to evaluate the protective effect of methanol garlic extract on the enzymes related with polyol pathway, advanced glycation end products, markers of oxidative stress and antioxidant status in brain of streptozotocin induced diabetic rats. Antioxidant capability of methanol extract of garlic was evaluated by 2,2-diphenyl-2-picrylhydrazyl hydrate radical and FOX (ferrous ion oxidation-xylenol orange) H2O2 scavenging test. Diabetes was induced by single i.p injection of STZ (32mg/kg per body Wt.,). Blood glucose levels and body wt, were measured on every 7th day over a period of 30 days. The diabetic rats treated with garlic extract at two doses 250mg/kg and 500mg/kg body wt., by oral administration. Diabetic rats showed significant increase in food and water intake, decrease in blood glucose levels, body weights, but could not show any recovery by garlic treatment. Garlic treatment significantly decreased aldose reductase (AR); sorbitol dehydrogenase (SD) and glutathione S-transferase (GST) enzyme activities. A decrease of malndialdehyde (MDA), Protein carbonyls (PC), Pentosidine advance oxidation protein products (AOPP), Advanced glycation end products (AGEs) was also observed. Additionally garlic administration produced a restoration of brain superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) in diabetic rats. Garlic treatment also reduced the free radical formation and progression of hyperglycemia induced diabetic complications by decreasing influx of glucose into the polyol pathway and increased activity of antioxidant enzymes. The current study reveals exerts, efficiently, an attenuating effect of methanol garlic extract exterted antihyperlgycemic, antioxidant and anti-glycating effects in a dose dependent manner in diabetic rats.

Therapeutic insights of hydromehtanolic leaf extract of Xanthium indi-cum and α-tocopherol in streptozotocin induced diabetic renal oxidative stress

2017 ◽  
Vol 6 (5) ◽  
pp. 158-166
Author(s):  
Anila Devi Meruva ◽  
Ravi Sahukari ◽  
Venkata Subbaiah Ganjikunta ◽  
Shanmugam Bhasha ◽  
Sathyavelu Reddy Kesireddy
Keyword(s):  
Blood Glucose ◽  
Serum Insulin ◽  
Microvascular Complications ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Insulin Levels ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Body Weights ◽  
Mda Content

Diabetic nephropathy (DN) is one of the important microvascular complications of diabetes mellitus. Several phytochemicals have been reported to show beneficial consequences in Xanthium indicum leaves and α-tocopherol is a well known antioxidant, putting a halt to the fatality of renal dysfunc-tions in DN. In the current study, seven groups of male albino rats six in each group, received the following treatment scheduled for 4 weeks: Normal control, Xanthium indicum, α-tocopherol, Diabetes control, Glibenclamaide treated diabetic, α-tocopherol treated diabetic and Xanthium treated diabetic. Evaluations were made for blood glucose levels, body weights, serum insulin levels, MDA content, creatintne in urine as well as in serum and the histopathological changes were monitored kidney tissues in all experimental rats. Blood glucose levels were significantly (***P<0.001) decreased whereas serum insulin levels and body weights were significantly (***P< 0.001) in-creased, MDA content, serum creatinine levels were significantly (***P< 0.01), (***P<0.001), decreased and urine creatinine levels were increased with the treatment of plant extract and α-tocopherol in diabetic rats. Overall, the findings of this study indicated that the hydromethanolic extract of X. indicum leaves and α-tocopherol possesses a potent capacity that attenuates the renal damage to minimize the deleterious effects of free radicals by maintaining renal hemodynamics in diabetic conditions probably through its antioxidative and hypoglycaemic activity.

Fenugreek seed extract ameliorates cognitive deficits in streptozotocin-induced diabetic rats

2019 ◽  
Vol 30 (4) ◽  
Author(s):  
Praveen K. Kodumuri ◽  
Christofer Thomas ◽  
Raghu Jetti ◽  
Anil Kumar Pandey
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Cognitive Dysfunction ◽  
Memory Impairment ◽  
Neuronal Loss ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
And Oxidative Stress

Abstract Background Natural medicinal plants have been the focus of current research for developing neuroprotective agents to be used in the diabetes-linked cognitive dysfunction. Trigonella foenum-graecum seeds (known as fenugreek, methi in Hindi), is a well-known traditional medicinal herb and possesses anti-diabetic, anti-oxidant, and anti-inflammatory properties. Purpose This study was undertaken to explore the ameliorative effects of T. foenum-graecum seed extract on diabetes-induced cognitive dysfunction. Methods Experimental diabetes was induced by administering a single dose of streptozotocin (60 mg/kg) through intraperitoneal dose. Cognitive function was assessed using a T-maze and the Morris water maze. Lipid peroxidation levels and oxidative stress in the hippocampus was measured. Quantification of hippocampal CA1 and CA3 regions was done using cresyl violet stain. Results Diabetic rats demonstrated learning and memory impairment, which was evident from poor performance in behavioral tasks, i.e. T-maze and Morris water maze tasks. Learning and memory impairment in diabetic animals is associated with increased blood glucose levels, increased oxidative stress in the hippocampus and decreased number of neurons in the CA1 and CA3 regions of the hippocampus. The diabetic rats administered with T. foenum-graecum showed improved performance in behavioral tasks, and these changes were associated with decreased blood glucose levels, decreased oxidative stress in the hippocampus, and decreased neuronal loss from the CA1 and CA3 regions of the hippocampus. Conclusion In conclusion, administration of T. foenum-graecum seed extract ameliorates diabetes-linked cognitive dysfunction in rats by decreasing blood glucose levels, reducing lipid peroxidation and oxidative stress in the hippocampus, and preventing neuronal loss from the hippocampus.

scholarly journals Changes in Oxidative Stress and Antioxidant Enzyme Activities in Streptozotocin-Induced Diabetes Mellitus in Rats: Role ofAlhagi maurorumExtracts

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
S. A. Sheweita ◽  
S. Mashaly ◽  
A. A. Newairy ◽  
H. M. Abdou ◽  
S. M. Eweda
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Glucose Levels ◽  
Blood Glucose Levels

Alhagi maurorum(camel thorn plant) is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. No previous study has been conducted before onA. maurorum extractsas an antioxidative stress and/or antidiabetic herb in STZ-induced DM in rats. Therefore, four groups of rats were allocated as control (C), STZ-induced DM (D), and STZ-induced DM supplemented with 300 mg/kg BW of either aqueous extract (WE) or ethanolic extract (EE) ofA. maurorum. The plasma levels of glucose, TG, TC, LDL-C and VLDL-C, MDA, and bilirubin and the activities of transaminases and GR were significantly increased in the diabetic group. Also, diabetic rats showed severe glucose intolerance and histopathological changes in their livers. In addition, levels of insulin, total proteins, GSH, and HDL-C and the activities of SOD, GPx, and GST were significantly decreased in the diabetic rats compared to those of the control group. The ingestion ofA. maurorumextracts lowered the blood glucose levels during the OGTT compared to the diabetic rats and restored all tested parameters to their normal levels with the exception of insulin level that could not be restored. It is concluded thatA. maurorumextracts decreased elevated blood glucose levels and hyperlipidemia and suppressed oxidative stress caused by diabetes mellitus in rats.

scholarly journals Effect of a novel succinamic acid derivative as potential anti-diabetic agent in experimental diabetic rats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 57-62
Author(s):  
Nikhil Khurana ◽  
Pankaj Sharma ◽  
Sunita Bhagat ◽  
Suman Bala Sharma
Keyword(s):  
Lipid Peroxidation ◽  
Serum Lipid ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Succinamic Acid ◽  
Oxobutanoic Acid

4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid which is a succinamic acid derivative has been synthesized in 3 step reaction with malic acid. Its structure confirmation was done by various techniques like 1H NMR, 13C NMR, & HRMS and is recently proposed as an insulinotropic agent for the treatment of non-insulin dependent diabetes mellitus. In the present study, the effect of 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid on plasma glucose, serum insulin, serum lipid profile and lipid peroxidation in streptozotocin–nicotinamide induced type 2 diabetic model was investigated.  4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid was administered orally (20 mg/kg b.w.) to streptozotocin + nicotinamide (STZ + NAD) induced diabetic rats for 28 days. A significant increase in fasting blood glucose levels, HbA1c levels, Serum lipid profile (TG & TC) and in  the levels of Malonaldialdehyde (MDA, end product of lipid peroxidation) was observed in STZ +NAD diabetic rats whereas the levels of high density lipoprotein-cholesterol (HDL-C) and serum insulin levels were significantly decreased  in STZ + NAD induced diabetic rats The effect of 4-((benzyloxy)amino)-2-hydroxy-4-oxobutanoic acid was compared with glibenclamide, a reference drug. Treatment with 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid and glibenclamide resulted in a significant reduction of fasting blood glucose levels with increase in plasma insulin levels in diabetic treated rats. 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid also resulted in a significant improvement in serum lipids and lipid peroxidation products. Our results suggest the potential role of 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid in the management of type-2 diabetes mellitus experimental rats. Keywords: 4-((benzyloxy) amino)-2-hydroxy-4-oxobutanoic acid, dyslipidemia, streptozotocin induced diabetes, lipid peroxidation

scholarly journals UPLC/ESI-MS Phytochemical Screening of Deverra tortuosa Haematological and Histopathological Studies and Streptozotocin-Induced Diabetes in Rat

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Fahad A. Alhumaydhi ◽  
Abdullah S. M. Aljohani ◽  
Eman R. Elsharkawy
Keyword(s):  
Blood Glucose ◽  
Blood Cells ◽  
Methanol Extract ◽  
Positive Impact ◽  
White Blood Cells ◽  
Diabetic Rats ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Histopathological Studies

Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of Deverra tortuosa in streptozotocin-induced diabetic rats were investigated in vivo. A single intraperitoneal injection of 160 mg/kg bodyweights of streptozotocin was used to cause diabetes. Blood glucose levels were tested with an AccuCheck Advantage II glucometer and blood glucose test strips. After diabetes was confirmed, animals were orally treated with the extract, metformin, and insulin according to the experimental design. After extract therapy, histological alterations in the pancreas of diabetic rats were investigated. When compared to a control group, daily oral administration of D. tortuosa extract (300 mg/kg body weight) plus metformin (100 mg/kg) had a positive effect on blood glucose levels as well as showed an increased number of white blood cells (WBCs) and red blood cells (RBCs). The treatment with the extract for two weeks showed a positive impact on pancreatic histopathological changes in the groups with the diabetic rats. Phenolic fraction of the methanol extract was screened by the liquid chromatography-mass spectroscopy (LC-MS) method, which unveiled the existence of flavonoid compounds and phenolics as kaempferol, rutin, isorhamnetin-3-O-rutinoside, caffeic acid, and 4-hydroxybenzoic acid 4-O-glucoside. The results confirmed the use of the plant as an antidiabetic agent; the research recommended further studies on the plant to use the plant as an antidiabetic drug, where the plant extract also showed improvement in blood parameters.

scholarly journals Hypoglycemic Activity and Antioxidative Stress of Extracts and Corymbiferin fromSwertia bimaculata In VitroandIn Vivo

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Zhaoxia Liu ◽  
Luosheng Wan ◽  
Yuedong Yue ◽  
Zuoqi Xiao ◽  
Yutang Zhang ◽  
...  
Keyword(s):  
Serum Insulin ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Glucose Consumption ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Alternative Agent

The present study was to investigate the anti-diabetic activities ofSwertia bimaculata. Based on the glucose consumption ofS. bimaculataextractsand different fractions (petroleum, dichloromethane, ethyl acetate,n-butanol and water extracts) in 3T3-L1 adipocyte assay, ethanol (ETH) and dichloromethane (DTH) extracts had the most effective potency. Furthermore, ETH, DTH and corymbiferin (the most abundant component of DTH) were evaluated for anti-diabetic effects in high fat and sucrose fed combined with low dose streptozocin induced diabetic rats. DTH and corymbiferin displayed remarkable anti-diabetic activities. The fasting blood glucose levels were significantly decreased, while the serum insulin levels were obviously increased. The oral glucose tolerance was also improved. The lowed serum total cholesterol, low density lipoprotein (LDL) and triglyceride levels and increased ratio of HDL (high density lipoprotein)/LDL were observed. The insulin sensitivity was improved on the basis of increased expressions of insulin-receptor substrate-2, phosphatidylinositol 3-kinase and Ser/Thr kinase AKT2. And also DTH and corymbiferin improved antioxidant capacity and carbohydrate metabolism in diabetic rats, along with the improvement of histopathology of livers and pancreaticβcells. Corymbiferin was one of active constituents, responsible for anti-diabetic properties. Therefore,S. bimaculatacould be considered as an alternative agent against diabetes mellitus.

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