scholarly journals Simultaneous Determination of Nε-(carboxymethyl) Lysine and Nε-(carboxyethyl) Lysine in Different Sections of Antler Velvet after Various Processing Methods by UPLC-MS/MS

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3316 ◽  
Author(s):  
Rui-ze Gong ◽  
Yan-hua Wang ◽  
Yu-fang Wang ◽  
Bao Chen ◽  
Kun Gao ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
G Protein ◽  
Processing Methods ◽  
Factors Affecting ◽  
Potential Consumer ◽  
Glycation End Products ◽  
Different Temperatures ◽  
Carboxymethyl Lysine ◽  
Harmful Effects

Nε-(Carboxymethyl) lysine (CML) and Nε-(carboxyethyl) advanced glycation end-products (AGEs) and are frequently used as markers of AGE formation. AGEs, such as CML and CEL, have harmful effects in the human body and have been closely linked to many diseases such as diabetes and uremia. However, details on the contents of CML and CEL after applying different antler velvet processing methods are lacking. In this research, a robust lysine (CEL) are two typical UPLC-MS/MS method has been developed for the simultaneous determination of CML and CEL in various sections of antler velvet processed with different methods. In addition, factors affecting the CML and CEL contents are discussed. The CML contents of antler velvet after freeze-drying, boiling, processing without blood, and processing with blood were 74.55–458.59, 119.44–570.69, 75.36–234.92, and 117.11–456.01 μg/g protein, respectively; the CEL contents were 0.74–12.66, 11.33–35.93, 0.00–6.75, and 0.00–23.41 μg/g protein, respectively. The different contents of CML and CEL in the different samples of antler velvet result from the different interactions of the protein and lysine at different temperatures. These data can be used to estimate the potential consumer intake of CML and CEL from antler velvet and for guiding producers on how to reduce the production of CML and CEL.

scholarly journals The Hydrophobic Effect in the Adsorption Process of Alkyltrimethylammonium Bromides on to Activated Carbon

1998 ◽  
Vol 16 (7) ◽  
pp. 557-564 ◽  
Author(s):  
Jinben Wang ◽  
Yao Lu ◽  
Buxing Han ◽  
Ming Dai ◽  
Haike Yan ◽  
...  
Keyword(s):  
Activated Carbon ◽  
Adsorption Process ◽  
Hydrophobic Effect ◽  
Carbon Surface ◽  
Thermodynamic Quantities ◽  
Factors Affecting ◽  
Alkyltrimethylammonium Bromides ◽  
Different Temperatures ◽  
Surface Measurements

This paper describes the hydrophobic effect in the process of adsorption of alkyltrimethylammonium bromides from aqueous solution on to an activated carbon surface. Measurements of the adsorption isotherms of a series of alkyltrimethylammonium bromides with different hydrocarbon tail lengths at the activated carbon surface were carried out. In order to investigate the temperature dependence of the adsorption process, the determination of the adsorption of cetyltrimethylammonium bromide (CTAB) was undertaken at different temperatures in the range 20–60°C. From the experimental results obtained, the relevant thermodynamic quantities have been calculated. The role played by the hydrophobic effect in the adsorption process of cationic surfactants on to activated carbon and the factors affecting the adsorption mechanism were discussed.

scholarly journals Importance of Advanced Glycation End Products (AGE) in Human Disease and Diagnosis

2018 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Reducing Sugars ◽  
Enzymatic Reaction ◽  
Advanced Glycation ◽  
High Risk Disease ◽  
Glycation End Products ◽  
End Products ◽  
Carboxymethyl Lysine ◽  
Method Of Measurement ◽  
Near Future

Advanced Glycation End (AGE) is a product of non-enzymatic reaction between reducing sugars of carbonyls and proteins. AGE accumulates with age. It may be derived from methylglyoxal (MG), glyoxal, and 3-deoxyglucosone (3DG) groups. AGEs are chemically heterogeneous group of compounds with only 25 AGE structures fully characterized. Among these Nε-carboxymethyl-lysine (CML) is the simplest and best characterized AGE. It is involved in the pathophysiological conditions like diabetes, cardiovascular diseases, atherosclerosis and aging disease like Alzheimer disease. So, it is important to measure the AGE level in human diseases. Skin autofluorescence (AF) is a noninvasive measurement of AGE accumulation. In this review, different methods of determination of AGE level in human will be discussed because of standard method of measurement of AGE have not been established yet. It is suggested that AGE may play an important in assessing diagnosis, prognosis and prevention of some high risk disease in near future.

scholarly journals Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Lilla Knels ◽  
Monika Valtink ◽  
Jamlec De la Vega Marin ◽  
Gerald Steiner ◽  
Cora Roehlecke ◽  
...  
Keyword(s):  
Annexin V ◽  
Vital Staining ◽  
Irradiation Damage ◽  
3T3 Fibroblasts ◽  
Resazurin Assay ◽  
Glycation End Products ◽  
Different Temperatures ◽  
Effects Of Temperature ◽  
Exact Temperature

Water-filtered infrared-A (wIRA) radiation has been described as supportive for tissue regeneration. We sought to investigate in detail the wIRA effect at different temperatures in 3T3 fibroblasts that were treated with glyoxal to induce formation of advanced glycation end products (AGEs). Nonirradiated and nonglyoxal-treated cells served as controls. Experiments were carried out over a range of 37∘–45∘C with exact temperature monitoring to distinguish between temperature and wIRA effects. Metabolic activity was assessed by resazurin assay. Mitochondrial membrane potential was assessed by JC-1 vital staining. Apoptotic changes were determined by vital staining with annexin V and YO-PRO-1 and determination of subG1 DNA content. Temperature had a dominant effect overriding effects exerted by wIRA or glyoxal treatment. The number of apoptotic cells was significantly higher at 45∘C, while the percentage of healthy cells was significantly lower at 45∘C. WIRA irradiation itself or in combination with glyoxal treatment exerted no damaging effects on the fibroblasts at physiological (37∘–40∘C) or higher (42∘–45∘C) temperatures compared to untreated controls. Temperatures of 45∘C, which can occur during inappropriate application of infrared irradiation, damage cells even in the absence of wIRA or glyoxal application.

scholarly journals A quantitative HPLC method for simultaneous determination of prodrug of voriconazole and voriconazole in beagle plasma, and its application to a toxicokinetic study

2021 ◽  
Author(s):  
Yufa Wen ◽  
Shuang Chen ◽  
Yanjuan Yuan ◽  
Qing Shao ◽  
Xuejun He ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Potassium Dihydrogen Phosphate ◽  
Internal Standard ◽  
Storage Conditions ◽  
Hplc Method ◽  
Dihydrogen Phosphate ◽  
Different Temperatures ◽  
Reproducible Method

AbstractA simple, rapid, efficient and reproducible method based on High Performance Liquid Chromatography (HPLC) for simultaneous determination of prodrug of voriconazole (POV) and voriconazole in beagle plasma has been established and validated. Omeprazole was utilized as the sole internal standard. Analytes and internal standards were extracted through protein precipitation and separated on a Venusil XBP C18 chromatography column (4.6 × 250 mm, 5 µm). The mobile phase was methanol and 20 mmol/L potassium dihydrogen phosphate. Chromatographic separation was achieved by using an isocratic elution procedure that used 65% methanol and a flow rate of 1 mL/min. The ultraviolet (UV) detection wavelength was 256 nm and the total running time was 15 min. This method showed good linear ranges of 100–75,000 ng/mL for voriconazole prodrug and 200–100,000 ng/mL for voriconazole respectively. The precision and accuracy were acceptable. Analytes in plasma samples are stable under different temperatures and storage conditions. The developed HPLC method has been successfully applied to the studies of toxicokinetics of POV after intravenous drip in beagle and provided important information for the further development and application.

scholarly journals Mathematical Model of New Type of Train Buffer Made of Polymer Absorber—Determination of Dynamic Impact Curve for Different Temperatures

Machines ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 47
Author(s):  
Hristijan Mickoski ◽  
Ivan Mickoski ◽  
Marjan Djidrov ◽  
Filip Zdraveski
Keyword(s):  
Mathematical Model ◽  
Influential Factors ◽  
Dynamic Impact ◽  
Factors Affecting ◽  
Impact Speed ◽  
Environment Temperature ◽  
Different Temperatures ◽  
New Type ◽  
The Impact

Previous experimental knowledge has confirmed that one of the most influential factors affecting the performance of polymer friction absorbers embedded in buffer housing as part of the buffer and chain coupler is the temperature. This paper defines a mathematical model of a friction-type polymer absorber, PMKP-110. The presented mathematical model specifically includes the influence of the environment temperature on the dynamic impact curve for −60 °C and 15 °C. The dependence between the initial pre-tension of the buffer and the ambient temperature is calculated. The model involves an equation of motion for moving parts of the absorber, and the solution of the differential equation is achieved in Matlab. Results are given as diagrams of the impact deformation and impact speed of the polymer block, with assumed zero initial impact speed. The model can be used to analyze the action of the longitudinal forces that occur during transient conditions of the movement of the carriages.

scholarly journals Multicomponent spectrometric analysis of riboflavin and photoproducts and their kinetic applications

2014 ◽  
Vol 12 (6) ◽  
pp. 635-642 ◽  
Author(s):  
Muhammad Sheraz ◽  
Sadia Kazi ◽  
Sofia Ahmed ◽  
Kiran Qadeer ◽  
Marium Khan ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Spectrometric Analysis ◽  
Factors Affecting ◽  
Kinetics Of Degradation ◽  
Analytical Procedures ◽  
Kinetics Of ◽  
Kinetic Treatment ◽  
Visible Spectrometry

AbstractRiboflavin (RF) is a light sensitive compound and is known to form a number of photoproducts. These photoproducts possess the same nucleus and may interfere in the analysis of RF by UV and visible spectrometry. Therefore, it is necessary to apply the methods of multicomponent spectrometric analysis to quantify the vitamin and its photoproducts accurately. Such methods are useful in the study of the kinetics of photodegradation reactions of RF to obtain accurate and reliable results. Any interference in these methods due to linear or nonlinear irrelevant absorption of the minor unknown products can be accounted for by the application of appropriate correction procedures prior to kinetic treatment. Various factors affecting the accuracy, precision and selectivity of these analytical procedures are also discussed. This review highlights the principles and applications of multicomponent spectrometric methods and their application to the simultaneous determination of RF and its major photoproducts in degraded solutions to evaluate the kinetics of degradation.

Simultaneous determination of N ε-(carboxymethyl) lysine and N ε-(carboxyethyl) lysine in cereal foods by LC–MS/MS

2013 ◽  
Vol 238 (3) ◽  
pp. 367-374 ◽  
Author(s):  
Jialiang He ◽  
Maomao Zeng ◽  
Zongpin Zheng ◽  
Zhiyong He ◽  
Jie Chen
Keyword(s):  
Simultaneous Determination ◽  
Carboxymethyl Lysine
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