scholarly journals Chemical Profile and Antibacterial Activity of a Novel Spanish Propolis with New Polyphenols also Found in Olive Oil and High Amounts of Flavonoids

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3318 ◽  
Author(s):  
María Coronada Fernández-Calderón ◽  
María Luisa Navarro-Pérez ◽  
María Teresa Blanco-Roca ◽  
Carolina Gómez-Navia ◽  
Ciro Pérez-Giraldo ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Olive Oil ◽  
Staphylococcus Epidermidis ◽  
Biological Activities ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Chemical Profile ◽  
Therapeutic Benefits ◽  
New Type ◽  
Ethanolic Extract Of Propolis

Propolis is a natural product obtained from hives. Its chemical composition varies depending on the flora of its surroundings, but nevertheless, common for all types of propolis, they all exhibit remarkable biological activities. The aim of this study was to investigate the chemical composition and antimicrobial activity of a novel Spanish Ethanolic Extract of Propolis (SEEP). It was found that this new SEEP contains high amounts of polyphenols (205 ± 34 mg GAE/g), with unusually more than half of this of the flavonoid class (127 ± 19 mg QE/g). Moreover, a detailed analysis of its chemical composition revealed the presence of olive oil compounds (Vanillic acid, 1-Acetoxypinoresinol, p-HPEA-EA and 3,4-DHPEA-EDA) never detected before in propolis samples. Additionally, relatively high amounts of ferulic acid and quercetin were distinguished, both known for their important therapeutic benefits. Regarding the antimicrobial properties of SEEP, the minimal inhibitory and bactericidal concentrations (MIC and MBC) against Staphylococcus epidermidis strains were found at the concentrations of 240 and 480 µg/mL, respectively. Importantly, subinhibitory concentrations were also found to significantly decrease bacterial growth. Therefore, the results presented here uncover a new type of propolis rich in flavonoids with promising potential uses in different areas of human health.

scholarly journals Chemical Composition of the Essential Oil and Antimicrobial Properties of Crude Extract From Tanacetum Corymbosum (L.) Shi. Bip.

2021 ◽  
Author(s):  
Alexandru Ciocarlan ◽  
Lucian Lupascu ◽  
Aculina Aricu ◽  
Ion Dragalin ◽  
Nina Ciocarlan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Chemical Composition ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Biological Activities ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Phytopathogenic Bacteria ◽  
Germacrene D

The GC-MS analysis of the essential oil from Tanacetum corymbosum revealed the presence of 38 compounds, including terpenes - germacrene D, (Z)-β-farnesene, g-elemene, β-caryophyllene, aliphatic - palmitic and linoleic fatty acids, fatty alcohol n-octadecanol, higher alkane n-heneicosane as the major constituents. The in vitro antimicrobial assessment of the ethanolic extract showed promising antibacterial/antifungal activities against five Gram-(+), Gram-(-) and phytopathogenic bacteria species and two fungi strains. The data obtained in this study may be useful both for researchers and for producers interested in new or less studied species of medicinal plants in healthcare and their biological activities.

scholarly journals Pharmacological Potential and Chemical Composition of Crocus sativus Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 10
Author(s):  
Olha Mykhailenko ◽  
Vilma Petrikaite ◽  
Michal Korinek ◽  
Fang-Rong Chang ◽  
Mohamed El-Shazly ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Crocus Sativus ◽  
Leaf Extracts ◽  
Hplc Fingerprint ◽  
Health Related ◽  
Pharmacological Potential

Crocus sativus L. (saffron) has been traditionally used as a food coloring or flavoring agent, but recent research has shown its potent pharmacological activity to tackle several health-related conditions. Crocus sp. leaves, and petals are the by-products of saffron production and are not usually used in the medicine or food industries. The present study was designed to determine the chemical composition of the water and ethanolic extracts of C. sativus leaves and test their cytotoxic activity against melanoma (IGR39) and triple-negative breast cancer (MDA-MB-231) cell lines by MTT assay. We also determined their anti-allergic, anti-inflammatory, and anti-viral activities. HPLC fingerprint analysis showed the presence of 16 compounds, including hydroxycinnamic acids, xanthones, flavonoids, and isoflavonoids, which could contribute to the extracts’ biological activities. For the first time, compounds such as tectoridin, iristectorigenin B, nigricin, and irigenin were identified in Crocus leaf extracts. The results showed that mangiferin (up to 2 mg/g dry weight) and isoorientin (8.5 mg/g dry weight) were the major active ingredients in the leaf extracts. The ethanolic extract reduced the viability of IGR39 and MDA-MB-231 cancer cells with EC50 = 410 ± 100 and 330 ± 40 µg/mL, respectively. It was more active than the aqueous extract. Kaempferol and quercetin were identified as the most active compounds. Our results showed that Crocus leaves contain secondary metabolites with potent cytotoxic and antioxidant activities.

scholarly journals Chemical Composition, Antifungal, and Cytotoxicity Activities of Inga laurina (Sw.) Willd Leaves

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Carla de Moura Martins ◽  
Sérgio A. L. de Morais ◽  
Mário M. Martins ◽  
Luís C. S. Cunha ◽  
Cláudio V. da Silva ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Gallic Acid ◽  
Total Phenolics ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Vero Cells ◽  
Quercetin Glycosides ◽  
Cytotoxicity Activities ◽  
First Time

The species Inga laurina is native to the Brazilian Cerrado. There are no studies about the chemical composition and biological activities of extracts of this endangered species. The ethanolic extract and its successive fractions are rich in phenolic compounds and presented good antifungal activities. HPLC/MS-MS/MS and H1/C13 analysis led to the identification of seventeen compounds, most of which are gallic acid derivatives, myricetin and quercetin glycosides. The ethyl acetate fraction (EAF) contained high levels of total phenolics, expressed in milligrams of gallic acid equivalents per gram of extract (475.3 ± 1.9 mg GAE gextract-1) and flavonoids expressed in milligrams of quercetin equivalents per gram of extract (359.3 ± 10.6 mg QE gextract-1). This fraction was active against fungi of the Candida genus. The EAF showed MIC value 11.7 μg mL−1 against C. glabrata and a selectivity index of 1.6 against Vero cells. The flavonol glycoside myricetin-3-O-rhamnoside was isolated for the first time from the Inga laurina. These results make I. laurina a promising plant as a source of pharmaceutical and biological active antifungal compounds.

scholarly journals In Vitro Antifungal and Antivirulence Activities of Biologically Synthesized Ethanolic Extract of Propolis-Loaded PLGA Nanoparticles against Candida albicans

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Anupon Iadnut ◽  
Ketsaya Mamoon ◽  
Patcharin Thammasit ◽  
Sudjai Pawichai ◽  
Singkome Tima ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Virulence Factors ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Vero Cells ◽  
Plga Nanoparticles ◽  
Water Soluble ◽  
Free Form ◽  
Ethanolic Extract Of Propolis

Propolis is a natural substance and consists of bioactive compounds, which gives it antioxidant and antimicrobial properties. However, the use of propolis is limited by the low solubility in aqueous solutions. Thus, nanoparticles may be likely to accomplish enhanced delivery of poorly water-soluble phytomedicine. The aim of the present study was to fabricate and evaluate the biological activity of ethanolic extract of propolis-loaded poly(lactic-co-glycolic acid) nanoparticles (EEP-NPs). The EEP-NPs were prepared using the oil-in-water (o/w) single-emulsion solvent evaporation technique. The physicochemical properties of EEP-NPs were characterized and tested on their cytotoxicity, antifungal activity, and impact on key virulence factors that contribute to pathogenesis of C. albicans. EEP-NPs were successfully synthesized and demonstrated higher antifungal activity than EEP in free form. Moreover, EEP-NPs exhibited less cytotoxicity on Vero cells and suppressed the virulence factors of C. albicans, including adhesion, hyphal germination, biofilm formation, and invasion. Importantly, EEP-NPs exhibited a statistical decrease in the expression of hyphal adhesion-related genes, ALS3 and HWP1, of C. albicans. The results of this study revealed that EEP-NPs mediates a potent anticandidal activity and key virulence factors by reducing the gene-encoding virulence-associated hyphal- adhesion proteins of C. albicans and, thereby, disrupting the morphologic presence and attenuating their virulence.

scholarly journals Chemical Composition, Antioxidant, Antimicrobial and Antidiabetic Potential of Philodendron Bipinnatifidum Schott ex Endl

2019 ◽  
Vol 8 (2) ◽  
pp. 103-111
Author(s):  
Jaqueline Scapinello ◽  
Monica S. Z. Schindler ◽  
Leila Zanatta ◽  
Laura Cassol Mohr ◽  
Ana Paula Capelezzo ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
South American ◽  
High Antimicrobial Activity ◽  
Popular Medicine

Many of the species used in popular medicine do not have their biological activities already proven by scientific studies. Among these species, the endemic South American Philodendron bipinnatifidum Schott ex Endl deserves special attention since it is already in use in popular medicine for inflammation cases, such as erysipelas, orchitis and ulcers. This study evaluated the antioxidant, antimicrobial and antidiabetic activities of extracts of the hastes de P. bipinnatifidum. The ethanolic extract showed a significant antioxidant potential. The ethyl acetate extract resulted in high antimicrobial activity against Streptococcus pyogenes. The most significant biological activity of ethyl acetate extract relates to its chemical composition when compared with ethanolic extract, which showed the highest concentration of bioactive compounds. In vitro antidiabetic activity was only evaluated for ethyl acetate extract, resulting in inhibition of intestinal disaccharidases (maltase and sucrase) at concentration of 500 μg/mL.

scholarly journals Chemistry, biological activities and toxic effects of alkaloidal constituents of genus Delphinium - A mini review

2021 ◽  
Vol 10 (4) ◽  
pp. 486-499
Author(s):  
Mohammadreza Lotfaliani ◽  
Seyed Abdulmajid Ayatollahi ◽  
Farzad Kobarfard ◽  
Mustafa ghanadian ◽  
Pardis Mohammadi Pour
Keyword(s):  
Chemical Composition ◽  
North America ◽  
Biological Activities ◽  
Toxic Effects ◽  
Cytotoxic Activities ◽  
Diterpenoid Alkaloids ◽  
Medicinal Uses ◽  
Therapeutic Benefits ◽  
The Family ◽  
Alkaloidal Constituents

The genus Delphinium is one of the essential members of the family Ranunculaceae. These species grow wild in North America, Europe, and Asia. They have demonstrated antioxidant, antimicrobial, and cytotoxic activities. Diterpenoid alkaloids are their main constituents and seem to be responsible for medicinal and toxic properties. The primary purpose of this paper is to review the therapeutic benefits of Delphinium species, chemical composition, and its medicinal uses, in addition to the reported toxic effects of these plants influencing different animals and humans.

scholarly journals Antimicrobial activity of a novel Spanish propolis against planktonic and sessile oral Streptococcus spp

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Luisa Navarro-Pérez ◽  
Virginia Vadillo-Rodríguez ◽  
Irene Fernández-Babiano ◽  
Ciro Pérez-Giraldo ◽  
M. Coronada Fernández-Calderón
Keyword(s):  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Oral Streptococcus ◽  
Ancient Times ◽  
Ethanolic Extract Of Propolis

AbstractIncreased bacterial resistance to traditional antimicrobial agents has prompted the use of natural products with antimicrobial properties such as propolis, extensively employed since ancient times. However, the chemical composition of propolis extracts is extremely complex and has been shown to vary depending on the region and season of collection, due to variations in the flora from which the pharmacological substances are obtained, being therefore essential for their antimicrobial activity to be checked before use. For this purpose, we evaluate the in vitro antimicrobial and anti-biofilm activity of a new and promising Spanish ethanolic extract of propolis (SEEP) on Streptococcus mutans and Streptococcus sanguinis, responsible, as dominant ‘pioneer’ species, for dental plaque. Results reveal that S. sanguinis is more sensitive to SEEP, slowing and retarding its growth considerably with lower concentrations than those needed to produce the same effect in S. mutans. SEEP presents concentration- and time-dependent killing activity and, furthermore, some of the subinhibitory concentrations employed increased biofilm formation even when bacterial growth decreased. Mono and dual-species biofilms were also inhibited by SEEP. Findings obtained clearly show the relevance of using biofilm and subinhibitory concentration models to determine optimal treatment concentrations.

scholarly journals A Review on Avocado Seed: Functionality, Composition, Antioxidant and Antimicrobial Properties

2019 ◽  
pp. 1-10 ◽  
Author(s):  
Tassew Belete Bahru ◽  
Zinabwa Hailu Tadele ◽  
Eyasu Gebrie Ajebe
Keyword(s):  
Chemical Composition ◽  
Total Phenolics ◽  
Biological Activities ◽  
Antimicrobial Properties ◽  
Review Article ◽  
The Other ◽  
Edible Part ◽  
Medicinal Use ◽  
Ecological Problems ◽  
Avocado Seed

The seed of avocado is considered as one of the non-edible part of the fruit, which are usually discarded as residues and can cause ecological problems. Exploring the possible dietary and therapeutic potentials of especially such underutilized wastes will in addition reduce the possible environmental waste burden. The objectives of this review article is to alert the functionality, chemical composition, antioxidant and antimicrobial properties of avocado seed for its use as food and justification for its medicinal use. The composition like proximate (protein, fat, ash, moisture, fiber and carbohydreate), minerals (Ca, Zn, K, Na, P, Fe, Cu, Pb and Co), phytochemicals (Flavinoid, Tanine, Saponine, Total phenolics, Antioxidnt capacity, Oxalates, Phytates, Alkaloids) and Vitamins (A, B1, B2, B3, C and E) were reported to be present in the avocado seed in different proportions. Some poisonous phytochemicals are present in fresh seeds of avocado and not in the dry seeds, hence recommended to dry the seeds before processing for consumption. Its biological activities such as antioxidant, antihypertensive, fungicidal, larvicidal, hypolipidemic, amoebicidal and giardicidal activities had reported. Therefore, it can be concluded that, the avocado seed is nutritionally valuable as the other parts of the plant based on the phytochemical and nutrients it constitutes. The consumption of avocado seed is recommendable since it has high nutritional contents that make it enough for possible dietary and ethno-medicinal use.

scholarly journals Antimicrobial evaluation of flowering stalks of Cistanche violacea, a holoparasitic plant collected from arid region in Qassim, Saudi Arabia

2017 ◽  
Vol 4 (6) ◽  
pp. 239
Author(s):  
Emad Mohamed Abdallah
Keyword(s):  
Saudi Arabia ◽  
Staphylococcus Epidermidis ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Antimicrobial Properties ◽  
Bacterial Isolates ◽  
Antimicrobial Potential ◽  
Antimicrobial Evaluation ◽  
Inhibition Zones ◽  
Ethanol Extracts

Objective: Cistanche violacea has been found in Qassim region (Saudi Arabia), growing as a parasitic plant on Haloxylon salicornicum. The flowering stalk part of this plant was evaluated for antimicrobial potential against various referenced bacteria and fungi.Methods: The flowering stalk parts were collected from the field, dried, extracted by maceration in 80% methanol and 80% Ethanol, The crude extracts were examined against the tested microorganisms using agar-well diffusion method.Results: The antimicrobial testing showed that among 10 referenced bacterial isolates, only one exhibited significant susceptibility to the methanol and ethanol extracts of C. violacea, which was Staphylococcus epidermidis ATCC® 12228TM, recorded 15 and 16 mm inhibition zones, respectively. While other bacteria recorded weak or no susceptibility and no antifungal activity was detected.Conclusions: In general, the methanolic and ethanolic extracts of the flowering stalks of C. violacea exhibited weak or no antimicrobial properties except with one bacterial strain, further studies on the other biological activities are recommended as well as investigating the chemical constituents. To the best of the author’s knowledge, this is the first report evaluating the antimicrobial potential of the flowering stalks of C. violacea in Qassim area, Saudi Arabia.

scholarly journals Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum

2014 ◽  
Vol 50 (2) ◽  
pp. 309-320 ◽  
Author(s):  
Alessandra Tesch da Silva ◽  
Ewelyne Miranda de Lima ◽  
Isabela Faco Caliman ◽  
Leonardo Luiz Souza Porto ◽  
Andrews Marques do Nascimento ◽  
...  
Keyword(s):  
Vascular Reactivity ◽  
Inorganic Material ◽  
Micronucleus Test ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Hypotensive Effect ◽  
Chemical Profile ◽  
Response Curves ◽  
Cytotoxic Effects ◽  
Alpinia Purpurata

The aims of this study were to evaluate the chemical profile, vascular reactivity, and acute hypotensive effect (AHE) of the ethanolic extract of leaves of Alpinia purpurata (Vieill) K. Schum (EEAP). Its chemical profile was evaluated using HPLC-UV, ICP-OES, and colorimetric quantification of total flavonoids and polyphenols. The vascular reactivity of the extract was determined using the mesenteric bed isolated from WKY. AHE dose-response curves were obtained for both EEAP and inorganic material isolated from AP (IAP) in WKY and SHR animals. Cytotoxic and mutagenic safety levels were determined by the micronucleus test. Rutin-like flavonoids were quantified in the EEAP (1.8 ± 0.03%), and the total flavonoid and polyphenol ratios were 4.1 ± 1.8% and 5.1 ± 0.3%, respectively. We observed that the vasodilation action of EEAP was partially mediated by nitric oxide (·NO). The IAP showed the presence of calcium (137.76 ± 4.08 μg mg-1). The EEAP and IAP showed an AHE in WKY and SHR animals. EEAP did not have cytotoxic effects or cause chromosomic alterations. The AHE shown by EEAP could result from its endothelium-dependent vascular action. Rutin-like flavonoids, among other polyphenols, could contribute to these biological activities, and the calcium present in EEAP could act in a synergistic way.

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