Antimicrobial evaluation of flowering stalks of Cistanche violacea, a holoparasitic plant collected from arid region in Qassim, Saudi Arabia

Objective: Cistanche violacea has been found in Qassim region (Saudi Arabia), growing as a parasitic plant on Haloxylon salicornicum. The flowering stalk part of this plant was evaluated for antimicrobial potential against various referenced bacteria and fungi.Methods: The flowering stalk parts were collected from the field, dried, extracted by maceration in 80% methanol and 80% Ethanol, The crude extracts were examined against the tested microorganisms using agar-well diffusion method.Results: The antimicrobial testing showed that among 10 referenced bacterial isolates, only one exhibited significant susceptibility to the methanol and ethanol extracts of C. violacea, which was Staphylococcus epidermidis ATCC® 12228TM, recorded 15 and 16 mm inhibition zones, respectively. While other bacteria recorded weak or no susceptibility and no antifungal activity was detected.Conclusions: In general, the methanolic and ethanolic extracts of the flowering stalks of C. violacea exhibited weak or no antimicrobial properties except with one bacterial strain, further studies on the other biological activities are recommended as well as investigating the chemical constituents. To the best of the author’s knowledge, this is the first report evaluating the antimicrobial potential of the flowering stalks of C. violacea in Qassim area, Saudi Arabia.

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THE EFFECTIVENESS TEST OF MATOA (PometiaPinnata) ENDOPHYTIC BACTERIA TOWARDS BACTERIA NOSOCOMIAL INFECTION

Journal of Health, Technology and Science (JHTS) ◽

10.47918/jhts.v1i1.23 ◽

2020 ◽

Vol 1 (1) ◽

pp. 37-40

Author(s):

Denny Chandra Halid

Keyword(s):

Pseudomonas Aeruginosa ◽

Nosocomial Infection ◽

Staphylococcus Epidermidis ◽

Endophytic Bacteria ◽

Positive Control ◽

Negative Control ◽

Bacterial Isolates ◽

Series Design ◽

Inhibition Zones ◽

The One

This study aims to determine the effectiveness of Matoa (Pometiapin-nata) endophytic bacteria towards bacteria nosocomial infection namely Pseudomonas aeruginosa and Staphylococcus epidermidis. The subjects in the study were Matoa plant endophytic bacterial isolates on the stem (tw-igs). The positive control used is meropenem & negative control of aquades. This type of research uses quasi-experiments with a research design us-ing the One-Group Time-Series Design. The result of the study shows that there are 2 endophytic bacterial iso-lates in Matoa plants namely BEM 1 and BEM 2. Both endophytic bacterial isolates can kill and inhibits bacterial nosocomial infections Pseudomonas aeruginosa and Staphylococcus epider-midis with inhibition zones in the range of 16mm-22mm with a strong category very strong that it has the po-tential to be used as an antibacterial

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Isolation of Lasiodiplodin and Evaluation of some Biological Activities of the Stem Barks of Phyllanthus Albizzioides (Kurz) Hook.f.

SciMedicine Journal ◽

10.28991/scimedj-2019-0104-5 ◽

2019 ◽

Vol 1 (4) ◽

pp. 199-216 ◽

Cited By ~ 4

Author(s):

Khine Zar Wynn Lae ◽

Su Swe Su ◽

Nwet Nwet Win ◽

Ni Ni Than ◽

Hla Ngwe

Keyword(s):

Biological Activities ◽

Chemical Constituents ◽

Ethanol Extract ◽

2D Nmr ◽

Stem Bark ◽

Chloroform Extract ◽

Traditional System ◽

Spectroscopic Analyses ◽

Ethanol Extracts ◽

Relative Structure

A large number of the plants are claimed to possess the antibiotic properties in the traditional system and are also used extensively by the tribal people throughout the world. It is now mostly thought that nature has given the cure of every disease in one form or another. Plants have been known to cure people from various diseases in Ayurveda. This research focused on the screening of phytochemicals and some biological activities of Phyllanthus albizzioides. The research showed that the ethanol extracts were found to be greater chemical constituents than watery extracts. Among the chemical constituents, steroid compounds were observed in highest amount in ethanol extract and the second highest in flavonoid compounds followed by tannin compounds and pheolic compounds. Furthermore, the ethanol extracts were more effective than the watery extracts in all tested biological activities such as antioxidant, anti-diabetic, cytotoxicity, antimicrobial, antitumor and NO inhibition activities. A cyclohexanone compound was isolated from the chloroform extract of the stem bark of P. albizzioides. The relative structure was determined to be 4,5-dihydroxy-3-methyl-cyclohex-2-enone on the basic of HRESIMS, 1H NMR and 13C NMR spectroscopic analyses, including 2D NMR experiments.

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Phytochemical Screening, Phenolic Compounds Content, Antioxidant, Anti-inflammatory, and Antimicrobial Properties of Pachylobus balsamifera Guillaum from Gabon

Phytothérapie ◽

10.3166/phyto-2018-0059 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S65-S73 ◽

Cited By ~ 1

Author(s):

C. Sima-Obiang ◽

R.L. Ngoua-Meye-Misso ◽

G.R. Ndong-Atome ◽

J. Privat Ondo ◽

L.C. Obame-Engonga ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Activities ◽

Antimicrobial Properties ◽

Water Extract ◽

Antioxidant Property ◽

Salmonella Typhi ◽

Antimicrobial Activities ◽

Antimicrobial Potential ◽

Anti Inflammatory ◽

Ethanol Extracts

The goal of this work is to evaluate the antioxidant anti-inflammatory properties, the phenolic compounds content, and antimicrobial potential of water–acetone, water–ethanol, and water extracts of Pachylobus balsamifera. The phenolic compounds content was evaluated to estimate their effect on the antioxidant, anti-inflammatory, and antimicrobial potential of the plant. Antioxidant activities were examined by DPPH (2,2-diphenyl-1- picrylhydrazyl) and ABTS (2,2′-azino-bis) assays. Antiinflammatory activity was determined by the proteins denaturation inhibition method. All plant extracts were evaluated against six reference strains, eleven clinical isolates, and two fungal strains. Phenols content was highest in the water–acetone and water–ethanol extracts. The water extract showed strong anti-inflammatory effect. The water–acetone extract presented a strong antioxidant property and the highest antimicrobial activities against Pseudomonas aeruginosa, Acinetobacter baumannii, Salmonella Sp., and Neisseria meningitidis. The tested microorganisms showed sensitivity to all extracts of the plant with the exception of Escherichia coli 105182 CIP, Listeria innocua LMG 135668 BHI, Salmonella enterica, Salmonella typhi, and Neisseria gonorrhea. Our results suggest that Pachylobus balsamifera extracts contain antioxidant, anti-inflammatory, and antimicrobial properties.

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Antimicrobial properties and phytochemical constituents of the metha-nol extracts of Euphorbia retusa Forssk. and Euphorbia terracina L. from Saudi Arabia

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.4(2).p48-53 ◽

2014 ◽

Vol 4 (2) ◽

pp. 48-53

Author(s):

Emad M. Abdallah

Keyword(s):

Saudi Arabia ◽

Candida Albicans ◽

Bacillus Cereus ◽

Staphylococcus Epidermidis ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Klebsiella Pneumonia ◽

Methanol Extracts ◽

Phytochemical Constituents ◽

Antimicrobial Potency

This study aimed to investigate the phytochemical constituents and antimi-crobial activities of the crude methanol extracts of leaves of Euphorbia re-tusa Forssk and Euphorbia terracina L. collected from Saudi Arabia (Al-Rass province). Extracts of both plants showed presence of some bioactive phyto-chemicals such as tannins, saponins, terpenoids and traces of alkaloids for E. terracina; and tannins, saponins, flavonoids, alkaloids and terpenoids for E. retusa. Among investigated microorganisms, methanol extracts exhibited some degree of antimicrobial activity towards some microorganisms; name-ly,Candida albicans ATCC 1405, Staphylococcus epidermidis ATCC 49461, Bacillus cereus ATCC 10876 and Klebsiella pneumonia ATCC 27736 respec-tively for E. retusa and towards Staphylococcus aureus ATCC 25923.Staphylococcus epidermidis ATCC 49461,Candida albicans ATCC 1405,Bacillus cereus ATCC 10876 and Klebsiella pneumonia ATCC 27736 re-spectively for E. terracina. MIC testing was ranged from 100 to 6.2 mg/ml, proving the antimicrobial potency of these plants’ extracts against some mi-croorganisms. Further studies regarding purification and fractionation are required since the antimicrobial activities of tested extracts were not com-petitor when compared to the antibiotics Chloramphenicol 25mg/ml or Clot-rimazole 10 mg/ml.

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Antimicrobial potential of Polish propolis ethanol extracts towards Staphylococcus epidermidis biofilm

10.26226/morressier.5f3392ca9d1718ca4c8b2eff ◽

2020 ◽

Author(s):

Katarzyna Grecka

Keyword(s):

Staphylococcus Epidermidis ◽

Antimicrobial Potential ◽

Ethanol Extracts

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Synthesis and antimicrobial evaluation of novel quaternary ammonium salts against Staphylococcus Aureus in biofilm and planktonic form

10.5194/biofilms9-34 ◽

2020 ◽

Author(s):

Aneta Markova ◽

Michaela Hympanova ◽

Jan Marek ◽

Lukas Prchal

Keyword(s):

Quaternary Ammonium ◽

High Resolution Mass Spectrometry ◽

Antimicrobial Properties ◽

Quaternary Ammonium Salts ◽

Ammonium Salts ◽

Chronic Infections ◽

Research Activity ◽

Main Research ◽

Antimicrobial Potential ◽

Antimicrobial Evaluation

Introduction: Quaternary ammonium salts (QASs) are widely spread cationic surfactants applicable in various industrial branches. QASs manifesting antibacterial effect contain hydrophilic core and a long alkyl chain in the structure. These compounds have strong antimicrobial activity against certain bacteria strains especially non-spore forming, yeasts, or protozoa. S. Aureus&#160;is a frequent cause of nosocomial infections and&#160;plays an important role in the persistence of chronic infections, especially when it&#8217;s attached to medical implants and host tissue, and developed in mature biofilm.&#160;Either way, this pathogen represents a significant burden on the healthcare system and new antimicrobial compounds are always required in clinical practice. Hypothesis and aims: Our main research activity involves the synthesis of novel compounds based on QASs and evaluating their eradication ability against pathogenic S. Aureus in biofilm and free-floating form and subsequent effectivity comparison against these two different bacterial states.&#160; Methodology: All newly prepared compounds were prepared by the nucleophilic reaction, verified by Nuclear Magnetic Resonance (NMR) and High-Resolution Mass Spectrometry (HR-MS). The antimicrobial properties were evaluated by microdilution broth assay and by Calgary device based assay with MIC, MBC, and MBEC as outcomes. Results: Series of novel QAS were prepared and verified. The MICs, MBCs, and MBECs were measured for all substances and have shown antimicrobial potential against S. Aureus in both states, however higher concentration was needed for biofilm eradication. The activities of newly prepared compounds were comparable to QASs based disinfections already known and commonly used in practice. Conclusion: Series of synthesized compounds underwent antimicrobial evaluation and several compounds have shown antimicrobial potential against S. Aureus in planktonic and biofilm form.

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Chemical Profile and Antibacterial Activity of a Novel Spanish Propolis with New Polyphenols also Found in Olive Oil and High Amounts of Flavonoids

Molecules ◽

10.3390/molecules25153318 ◽

2020 ◽

Vol 25 (15) ◽

pp. 3318 ◽

Cited By ~ 1

Author(s):

María Coronada Fernández-Calderón ◽

María Luisa Navarro-Pérez ◽

María Teresa Blanco-Roca ◽

Carolina Gómez-Navia ◽

Ciro Pérez-Giraldo ◽

...

Keyword(s):

Chemical Composition ◽

Olive Oil ◽

Staphylococcus Epidermidis ◽

Biological Activities ◽

Antimicrobial Properties ◽

Ethanolic Extract ◽

Chemical Profile ◽

Therapeutic Benefits ◽

New Type ◽

Ethanolic Extract Of Propolis

Propolis is a natural product obtained from hives. Its chemical composition varies depending on the flora of its surroundings, but nevertheless, common for all types of propolis, they all exhibit remarkable biological activities. The aim of this study was to investigate the chemical composition and antimicrobial activity of a novel Spanish Ethanolic Extract of Propolis (SEEP). It was found that this new SEEP contains high amounts of polyphenols (205 ± 34 mg GAE/g), with unusually more than half of this of the flavonoid class (127 ± 19 mg QE/g). Moreover, a detailed analysis of its chemical composition revealed the presence of olive oil compounds (Vanillic acid, 1-Acetoxypinoresinol, p-HPEA-EA and 3,4-DHPEA-EDA) never detected before in propolis samples. Additionally, relatively high amounts of ferulic acid and quercetin were distinguished, both known for their important therapeutic benefits. Regarding the antimicrobial properties of SEEP, the minimal inhibitory and bactericidal concentrations (MIC and MBC) against Staphylococcus epidermidis strains were found at the concentrations of 240 and 480 µg/mL, respectively. Importantly, subinhibitory concentrations were also found to significantly decrease bacterial growth. Therefore, the results presented here uncover a new type of propolis rich in flavonoids with promising potential uses in different areas of human health.

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