scholarly journals Physicochemical Characterization and Paper Spray Mass Spectrometry Analysis of Myrciaria floribunda (H. West ex Willd.) O. Berg Accessions

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7206
Author(s):  
Yesenia Mendoza García ◽  
Ana Luiza Coeli Cruz Ramos ◽  
Afonso Henrique de Oliveira Júnior ◽  
Ana Cardoso Clemente Filha Ferreira de Paula ◽  
Angelita Cristine de Melo ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Physicochemical Characterization ◽  
Antimicrobial Properties ◽  
Mass Spectrometry Analysis ◽  
Total Phenolic ◽  
Pulp Yield ◽  
Average Weight ◽  
Spectrometry Analysis ◽  
Content Ratio ◽  
Ionization Mode

Myrciaria floribunda, also known as rumberry, is a tree native to the Brazilian Atlantic Forest, where its fruits have the potential for commercial use. This study evaluates the antioxidant potential, determines the phytochemical profile, and chemically characterizes the rumberry fruit. Accessions were sampled from the Rumberry Active Germplasm Bank of the Federal University of Alagoas, Brazil. Physical characteristics, chemical characteristics, and phenolic compound content were analyzed. Chemical profile characterization was carried out using PSMS. Accessions had an average weight of 0.86 g. Accession AC137 presented a higher pulp yield (1.12 g). AC132 and AC156 had larger fruits, AC137 showed greater firmness (5.93 N), and AC160 had a higher and total phenolic content ratio (279.01 ± 11.11). Orange-colored accessions scored higher in evaluated parameters, except for AC160 for phenolic content. Thirty-two compounds were identified on positive ionization mode and 42 compounds on negative ionization mode using PSMS. Flavonoids, followed by the derivatives of benzoic acid, sugars, and phenylpropanoids, were the most prominent. Myricitrin, quercitrin, and catechin stand out as flavonoids that have been reported in previous studies with antioxidant and antimicrobial properties, in addition to health and therapeutical benefits, demonstrating the potential of the rumberry fruit.

scholarly journals Chemical Characterization and Biological Properties of Royal Jelly Samples From the Mediterranean Area

2020 ◽  
Vol 15 (2) ◽  
pp. 1934578X2090808 ◽  
Author(s):  
Soukaïna El-Guendouz ◽  
Alexandra M. Machado ◽  
Smail Aazza ◽  
Badiaâ Lyoussi ◽  
Maria G. Miguel ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Total Protein ◽  
Royal Jelly ◽  
Chemical Characterization ◽  
Biological Properties ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Decenoic Acid ◽  
Spectrometry Analysis

Royal jelly (RJ) is a bee product that has high nutritional value and is beneficial for the human health, earning importance as a functional food. Thus, the characterization of its main biological properties is with high importance. In this work, 6 RJ samples obtained in Morocco, Portugal, and Spain were evaluated in terms of total phenol and flavone/flavonol contents; total protein; 10-hydroxy-2-decenoic acid (10-HDA); volatiles composition; antioxidant and anti-inflammatory properties; and inhibition of tyrosinase, xanthine oxidase (XO), and acetylcholinesterase (AChE) activities. Total phenolic content ranged from 3 to 9 mg gallic acid equivalent/g RJ, and flavone/flavonol content from 0.1 to 0.5 mg quercetin equivalent/g RJ. 10-Hydroxy-2-decenoic acid content varied from 0.9% to 1.2% and total protein from 5.5% to 29.7%. Gas chromatography-flame ionization detector and gas chromatography-mass spectrometry analysis showed RJ volatiles dominated by linolenic acid, 2-decenoic acid, and octanoic acid in variable amounts. The antioxidant activity was monitored through nitric oxide (NO) scavenging activity and hydrogen peroxide (H2O2) scavenging capacity, where the IC50 ranged from 2.3 to 3.4 and 0.2 to 1.5 mg/mL, respectively. Anti-AChE activity IC50 ranged from 0.7 to 4.6 mg/mL, while XO inhibition IC50 ranged from 3.3 to 11.9 mg/mL. The results showed that phenols and flavonoids highly contributed to the RJ biological properties in contrast to 10-HDA and proteins.

scholarly journals Effect of Chitosan–Tomato Plant Extract Edible Coating on the Quality, Shelf Life, and Antioxidant Capacity of Pork during Refrigerated Storage

Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 827 ◽  
Author(s):  
Saraí Chaparro-Hernández ◽  
Saul Ruiz-Cruz ◽  
Enrique Márquez-Ríos ◽  
José de Jesús Ornelas-Paz ◽  
Carmen L. Del-Toro-Sánchez ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Plant Extract ◽  
Tomato Plant ◽  
Microbial Population ◽  
Phenolic Content ◽  
Antimicrobial Properties ◽  
Edible Coating ◽  
Total Phenolic ◽  
Natural Extracts ◽  
Microbiological Analyses

The aim of this study was to evaluate the effectiveness of chitosan–tomato plant extract (C-TPE) edible coating (EC) on the physicochemical, microbiological, sensory, and antioxidant capacity changes of pork during storage. Edible coatings prepared with chitosan 1%, acetic acid 1%, glycerol, and TPE (0.1% and 0.3%) were tested. Slices of pork were submerged in different treatments (T1: C 1%; T2: C 1% + TPE 0.1%; T3: C 1% + TPE 0.3%; T4: control) and stored at 4 °C. The different treatments showed the best results in physicochemical and microbiological analyses, with reduced microbial population relative to the control. The highest antioxidant capacity and total phenolic content were shown in T3, and the overall acceptance was better in T2. The results show that the application of C with the addition of natural extracts, such as the tomato plant with antioxidant and antimicrobial properties, can be an alternative method for preserving pork.

scholarly journals Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae)

2020 ◽  
Vol 11 ◽  
Author(s):  
María José Larrazábal-Fuentes ◽  
Carlos Fernández-Galleguillos ◽  
Jenifer Palma-Ramírez ◽  
Javier Romero-Parra ◽  
Kevin Sepúlveda ◽  
...  
Keyword(s):  
Native Species ◽  
Total Flavonoid ◽  
Mass Spectrometry Analysis ◽  
Cholinesterase Inhibition ◽  
Total Phenolic ◽  
Protective Effects ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Spectrometry Analysis ◽  
Chemical Profiling

Artemisia copa Phil. (Asteraceae) (known as copa-copa) is a native species of Chile used as an infusion in traditional medicine by Atacameños people in the Altiplano, highlands of northern Chile. In this research, we have investigated for the first time the cholinesterase inhibition potential against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and the chemical profiling of the infusions prepared from the aerial parts of A. copa by high resolution spectrometry. In addition, total phenolic, total flavonoid content, antioxidant (DPPH, FRAP, and ORAC) and antiprozoal activity were tested. Artemisia copa showed good inhibitory activity against AChE and BChE (3.92 ± 0.08 µg/ml and 44.13 ± 0.10 µg/ml). The infusion displayed a total phenolics content of 155.6 ± 2.9 mg of gallic acid equivalents/g and total flavonoid content of 5.5 ± 0.2 mg quercetin equivalents/g. Additionally, trypanocidal activity against Trypanosoma cruzi was found (LD50 of 131.8 µg/ml). Forty-seven metabolites were detected in the infusion of A. copa including several phenolic acids and flavonoids which were rapidly identified using ultrahigh performance liquid chromatography orbitrap mass spectrometry analysis (UHPLC-Orbitrap-MS) for chemical profiling. The major compounds identified in the infusions were studied by molecular docking against AChE and BChE. The UHPLC-MS fingerprints generated can be also used for the authentication of these endemic species. These findings reveal that A. copa infusions can be used as beverages with protective effects.

scholarly journals Physicochemical characterization of fruits of Campomanesia guazumifolia (Cambess.) O. Berg (Myrtaceae)

2019 ◽  
Vol 41 ◽  
pp. e45923
Author(s):  
Jonas Goldoni ◽  
Clevison Luiz Giacobbo ◽  
Leandro Galon ◽  
Caroline Zarzzeka ◽  
Alison Uberti ◽  
...  
Keyword(s):  
Vitamin C ◽  
Physicochemical Characterization ◽  
Titratable Acidity ◽  
Calcium Content ◽  
Fruit Trees ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Pulp Yield ◽  
Native Flora ◽  
Protein Levels

Belonging to the family Myrtaceae, ‘sete capotes’ is a plant native to the Atlantic Forest biome. It produces yellow-green fruits between February and May. Although used for consumption by local populations, studies of the fruit are rare. The present work aimed at characterize the fruit in terms of physicochemical properties. We quantified mass, volume, moisture, pulp yield, pH, titratable acidity, soluble solids/titratable acidity, proteins, lipids, total sugars, reducing sugars, ash, macronutrients, micronutrients, pectin, vitamin C and phenolic compounds. Compared with commercial fruits and other native fruit trees, sete capotes is unusual for its pulp yield, vitamin C levels, total phenolic compound content, protein levels, calcium content, potassium levels and iron content. Through the results it is verified of the fruit as a functional food, mainly because of its nutritive content and because of its bioactive compounds. Our data emphasize the importance of study of the constituents of native flora.

scholarly journals Antioxidant and antimicrobial properties of ethanolic extracts of guarana, boldo, rosemary and cinnamon

2017 ◽  
Vol 20 (0) ◽  
Author(s):  
Jeannine Bonilla ◽  
Paulo Jose do Amaral Sobral
Keyword(s):  
Food Packaging ◽  
Phenolic Content ◽  
Antimicrobial Properties ◽  
Aureus Strain ◽  
Rosemary Extract ◽  
Total Phenolic ◽  
Food Industries ◽  
Cinnamon Extract ◽  
Active Food Packaging ◽  
Ethanolic Extracts

Abstract In this investigation, the ethanolic extracts of two less known plants, little reported in the literature (guarana and boldo leaves) were studied in comparison with the ethanolic extracts of two well studied plants (cinnamon and rosemary), regarding their colour, GC-MS profile, phenolic content and their antioxidant and antimicrobial properties. The rosemary (59.20 ± 0.28) and guarana (56.63 ± 0.54) extracts showed the highest values for luminosity (L*) and the UV-Vis absorption increased when L* decreased. GC-MS identified a limited number of compounds in the cinnamon and guarana extracts. The cinnamon extract showed the highest value for the total phenolic content (172 mg GA/g extract) as compared to the other extracts. The highest antioxidant capacity was observed for the boldo leaves extract in the TEAC (6.66 ± 0.17 mM) assay and for the rosemary extract in the DPPH (0.80 ± 0.14 mg/L) test. In addition, all the extracts showed antimicrobial activity against the S. aureus strain, indicating that all the extracts studied could be used by food industries to develop new active food packaging materials.

scholarly journals Physicochemical characterization, antioxidant activity and total phenolic content in 'Gala' apples subjected to different UV-C radiation doses

2017 ◽  
Vol 39 (1) ◽  
pp. 67
Author(s):  
Thaís Gabrielle Dias ◽  
Ana Carolina Vilas Boas ◽  
Maria Beatriz Affonso Junqueira ◽  
Luiz Carlos De Oliveira Lima
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Physicochemical Characterization ◽  
Total Phenolic ◽  
Radiation Doses ◽  
Uv C

scholarly journals Kainari, a Unique Greek Traditional Herbal Tea, from the Island of Lesvos: Chemical Analysis and Antioxidant and Antimicrobial Properties

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Evangelia Bampali ◽  
Konstantia Graikou ◽  
Nektarios Aligiannis ◽  
Ioanna Chinou
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Analysis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Total Phenolic ◽  
Herbal Tea

The chemical composition, as well as the total phenolic content (TPC) and the potential antioxidant and antimicrobial activity, of three Kainari-herbal tea samples from different areas of Lesvos Island (Greece) was evaluated. The rich aroma of the mixtures was studied through GC-MS, as well as through Headspace Solid-Phase Microextraction (HS-SPME)/GC-MS analyses. Cinnamon, clove, nutmeg, pepper, and ginger were identified as main ingredients, while, throughout the chemical analysis of the volatiles of one selected sample, several secondary metabolites have been isolated and identified on the basis of GC-MS as well as spectral evidence as eugenol, cinnamic aldehyde and myristicin, cinnamyl alcohol, alpha-terpinyl acetate, and β-caryophyllene. Furthermore, two food dyes, azorubine and amaranth, were also isolated and identified from the infusions. The total phenolic content was estimated and the free radical scavenging activity was determined by DPPH and ABTS assays and the antimicrobial activity of the extracts was tested showing a very interesting profile against all the assayed microorganisms. Due to its very pleasant aroma and taste properties as well as to its bioactivities, Kainari-herbal tea could be further proposed as functional beverage.

scholarly journals Dual-purpose isocyanides produced by Aspergillus fumigatus contribute to cellular copper sufficiency and exhibit antimicrobial activity

2021 ◽  
Vol 118 (8) ◽  
pp. e2015224118
Author(s):  
Nicholas Raffa ◽  
Tae Hyung Won ◽  
Andrew Sukowaty ◽  
Kathleen Candor ◽  
Chengsen Cui ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Inductively Coupled Plasma ◽  
Iron Homeostasis ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Biosynthetic Gene Cluster ◽  
Mass Spectrometry Analysis ◽  
Copper Binding ◽  
Spectrometry Analysis ◽  
Cellular Copper

The maintenance of sufficient but nontoxic pools of metal micronutrients is accomplished through diverse homeostasis mechanisms in fungi. Siderophores play a well established role for iron homeostasis; however, no copper-binding analogs have been found in fungi. Here we demonstrate that, in Aspergillus fumigatus, xanthocillin and other isocyanides derived from the xan biosynthetic gene cluster (BGC) bind copper, impact cellular copper content, and have significant metal-dependent antimicrobial properties. xan BGC-derived isocyanides are secreted and bind copper as visualized by a chrome azurol S (CAS) assay, and inductively coupled plasma mass spectrometry analysis of A. fumigatus intracellular copper pools demonstrated a role for xan cluster metabolites in the accumulation of copper. A. fumigatus coculture with a variety of human pathogenic fungi and bacteria established copper-dependent antimicrobial properties of xan BGC metabolites, including inhibition of laccase activity. Remediation of xanthocillin-treated Pseudomonas aeruginosa growth by copper supported the copper-chelating properties of xan BGC isocyanide products. The existence of the xan BGC in several filamentous fungi suggests a heretofore unknown role of eukaryotic natural products in copper homeostasis and mediation of interactions with competing microbes.

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