scholarly journals A Novel Hybrid Nanosystem Integrating Cytotoxic and Magnetic Properties as a Tool to Potentiate Melanoma Therapy

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 693 ◽  
Author(s):  
Nuno Cruz ◽  
Jacinta Oliveira Pinho ◽  
Graça Soveral ◽  
Lia Ascensão ◽  
Nuno Matela ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Cellular Proliferation ◽  
Health Concern ◽  
Major Health ◽  
Transmission Electron ◽  
Mean Size ◽  
Melanoma Treatment ◽  
The Mean ◽  
Hybrid Nanosystem ◽  
Melanoma Therapy

Cancer is a major health concern and the prognosis is often poor. Significant advances in nanotechnology are now driving a revolution in cancer detection and treatment. The goal of this study was to develop a novel hybrid nanosystem for melanoma treatment, integrating therapeutic and magnetic targeting modalities. Hence, we designed long circulating and pH-sensitive liposomes loading both dichloro(1,10-phenanthroline) copper (II) (Cuphen), a cytotoxic metallodrug, and iron oxide nanoparticles (IONPs). The synthetized IONPs were characterized by transmission electron microscopy and dynamic light scattering. Lipid-based nanoformulations were prepared by the dehydration rehydration method, followed by an extrusion step for reducing and homogenizing the mean size. Liposomes were characterized in terms of incorporation parameters and mean size. High Cuphen loadings were obtained and the presence of IONPs slightly reduced Cuphen incorporation parameters. Cuphen antiproliferative properties were preserved after association to liposomes and IONPs (at 2 mg/mL) did not interfere on cellular proliferation of murine and human melanoma cell lines. Moreover, the developed nanoformulations displayed magnetic properties. The absence of hemolytic activity for formulations under study demonstrated their safety for parenteral administration. In conclusion, a lipid-based nanosystem loading the cytotoxic metallodrug, Cuphen, and displaying magnetic properties was successfully designed.

scholarly journals MicroRNA Profiling of Exosomes Derived from Red Blood Cell Units: Implications in Transfusion-Related Immunomodulation

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Haobo Huang ◽  
Jinfeng Zhu ◽  
Liping Fan ◽  
Qiuyan Lin ◽  
Danhui Fu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Bioinformatic Analysis ◽  
Mirna Microarray ◽  
Qrt Pcr ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean ◽  
Polymerase Chain

Purpose. To elucidate the microRNAs existent in exosomes derived from stored red blood cell (RBC) unit and their potential function. Materials and Methods. Exosomes were isolated from the supernatant derived from stored RBC units by sequential centrifugation. Isolated exosomes were characterized by TEM (transmission electron microscopy), western blotting, and DLS (dynamic light scattering). MicroRNA (miRNA) microarray was performed to detect the expression of miRNAs in 3 exosome samples. Results revealed miRNAs that were simultaneously expressed in the 3 exosome samples and were previously reported to exist in mature RBCs. Functions and potential pathways of some detected miRNAs were illustrated by bioinformatic analysis. Validation of the top 3 abundant miRNAs was carried out by qRT-PCR (quantitative reverse transcription‐polymerase chain reaction). Results. TEM and DLS revealed the mean size of the exosomes (RBC-derived) as 64.08 nm. These exosomes exhibited higher abundance of short RNA than the long RNA. 78 miRNAs were simultaneously detected in 3 exosome samples and mature RBCs. Several biological processes might be impacted by these miRNAs, through their target gene(s) enriched in a particular signalling pathway. The top 3 (abundant) miRNAs detected were as follows: miR-125b-5p, miR-4454, and miR-451a. qRT-PCR revealed higher abundance of miR-451a than others. Only miR-4454 and miR-451a abundance tended to increase with increasing storage time. Conclusion. Exosomes derived from stored RBC units possessed multiple miRNAs and, hence, could serve various functions. The function of exosomes (RBC-derived) might be implemented partly by the predominantly enriched miR-451a.

Surface Modification and Preparation of High Purity Nanometer Magnesium Hydroxide Using Impinging Stream

2011 ◽  
Vol 675-677 ◽  
pp. 847-851
Author(s):  
Jian Hai Zhao ◽  
Yan Ping Lai
Keyword(s):  
Surface Modification ◽  
Magnesium Hydroxide ◽  
High Purity ◽  
X Ray Diffraction ◽  
Control Factors ◽  
Operation Conditions ◽  
Crystallization Method ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean

High purity nanometer magnesium hydroxide is produced by impinging stream reaction crystallization method using bischofite as feedstock. Effects of operation conditions on the impinging stream of Mg (OH)2 are reported and the control factors of purity are confirmed. The morphology of the powder was characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Result shows that purity of Mg (OH)2 can reach 99% and the mean size of crystal is 13.5nm. Surface modification of nano-sized magnesium hydroxide using four surface modifiers such as sodium steatite, sodium laurylsulfonate, sodium oleate and sodium silicate were investigated in this paper. The modified magnesium hydroxide has smaller particle size, larger powder contact angle, slower sedimentation velocity, the less in-oil capacity than unmodified sample

Magnetic Properties of Magnetic Hydrogels

2015 ◽  
Vol 233-234 ◽  
pp. 310-313
Author(s):  
L.V. Nikitin ◽  
A.A. Gladkov ◽  
A.E. Korovushkin
Keyword(s):  
Magnetic Properties ◽  
Coercive Field ◽  
Remanent Magnetization ◽  
Magnetic Particles ◽  
Polyacrylamide Gel ◽  
Magnetization Curves ◽  
Iron Particles ◽  
Mean Size ◽  
The Mean

Several samples of magnetic hydrogels (also known as ferrogels) are prepared, and their magnetic properties are examined. Polyacrylamide gel is used as the base. Gel matrices are filled with the nanopowder: iron particles with the mean size about 30 nm. The magnetization curves of the samples are measured at different stages of de-swelling. The dependencies of such parameters of magnetization curves as coercive field, remanent magnetization from concentration of magnetic particles in the sample along the process of drying were investigated.

Preparation of Paclitaxel-Loaded Microspheres with Fe3O4 Nanoparticles and Study of their Target-Orientation and Sustained-Release Property

2012 ◽  
Vol 535-537 ◽  
pp. 521-524
Author(s):  
Sheng Cui ◽  
Ben Lan Lin ◽  
Xiao Dong Shen
Keyword(s):  
Sustained Release ◽  
Loading Rate ◽  
Drug Loading ◽  
Target Orientation ◽  
Mtt Method ◽  
Transmission Electron ◽  
Release Property ◽  
Mean Size ◽  
Magnetic Fe3o4 Nanoparticles ◽  
The Mean

Paclitaxel-loaded microspheres with magnetic Fe3O4 nanoparticles were synthesized with albumin as carrier. The morphology was characterized by high-resolution transmission electron microscopy (HRTEM). The drug-loading rate and entrapment rate of the microspheres were researched by ultraviolet and visible spectrophotometer. The inhibition action of taxol microspheres to growth of esophagus cancer Eca109 cell were examined by MTT method. The result shows that it is regular spherical. The mean size and size distribution of microspheres are 275.6nm and 144.3nm by Zetasizer3000. The drug-loading rate is 3.013% and the entrapment rate is 35.26%. It has excellent target-orientation and sustained-release property which can prevent the growth of Eca109 cell.

Weight gain in antipsychotic-naive patients: a review and meta-analysis

2009 ◽  
Vol 40 (2) ◽  
pp. 187-200 ◽  
Author(s):  
I. Tarricone ◽  
B. Ferrari Gozzi ◽  
A. Serretti ◽  
D. Grieco ◽  
D. Berardi
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
High Probability ◽  
Drug Exposure ◽  
Meta Analysis ◽  
Health Concern ◽  
Adjunctive Treatment ◽  
Review Of The Literature ◽  
Major Health ◽  
The Mean

BackgroundWeight gain is a long-recognized side-effect of antipsychotic (AP) drugs and a major health concern in the treatment of psychosis. The strength of the causal relationship between AP drug exposure and weight gain can only be gauged by a drugs trial conducted on AP-naive patients.MethodWe conducted a review of the literature regarding the amount of weight gain induced by APs in AP-naive patients and carried out a meta-analysis of mean weight gains.ResultsWe found 11 primary studies reporting the effects of APs on body weight or body mass index (BMI) in AP-naive patients. The mean body weight and BMI gains in AP-naive patients were highly significant from the first weeks of treatment. When we limited the analysis to studies conducted on patients hospitalized and without any adjunctive treatment potentially affecting weight, the resultant sample showed less heterogeneity and confirmed the final picture of weight gain at around 3.8 kg and 1.2 points BMI.ConclusionsWeight gain associated with AP therapy in AP-naive patients occurs rapidly in the first few weeks and continues during the following months. Clinicians should be aware of the high probability of causing weight gain in AP-naive patients and should strictly monitor such patients.

scholarly journals Antifungal and Anti-Inflammatory Potential of Bupleurum rigidum subsp. paniculatum (Brot.) H.Wolff Essential Oil

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 592
Author(s):  
Mónica Zuzarte ◽  
Pedro M. P. Correia ◽  
Jorge M. Alves-Silva ◽  
Maria J. Gonçalves ◽  
Carlos Cavaleiro ◽  
...  
Keyword(s):  
Essential Oil ◽  
Fungal Infections ◽  
Health Concern ◽  
Major Health ◽  
Transmission Electron ◽  
Anti Inflammatory ◽  
Bioactive Potential ◽  
Effective Drugs ◽  
First Time ◽  
Oxide Inhibition

Fungal infections remain a major health concern with aromatic plants and their metabolites standing out as promising antifungal agents. The present study aims to assess, for the first time, the antifungal and anti-inflammatory potential of Bupleurum subsp. paniculatum (Brot.) H.Wolff essential oil from Portugal. The oil obtained by hydrodistillation and characterized by GC-MS, showed high amounts of monoterpene hydrocarbons, namely α-pinene (29.0–36.0%), β–pinene (26.1–30.7%) and limonene (10.5–13.5%). The antifungal potential was assessed, according to CLSI guidelines, against several clinical and collection strains. The essential oil showed a broad fungicidal effect being more potent against Cryptococcus neoformans and dermatophytes. Moreover, a significant germ tube inhibition was observed in Candida albicans as well as a disruption of mature biofilms, thus pointing out an effect of the oil against relevant virulent factors. Furthermore, fungal ultrastructural modifications were detected through transmission electron microscopy, highlighting the nefarious effect of the oil. Of relevance, the oil also evidenced anti-inflammatory activity through nitric oxide inhibition in macrophages activated with lipopolysaccharide. In addition, the essential oil’s bioactive concentrations did not present toxicity towards macrophages. Overall, the present study confirmed the bioactive potential of B. rigidum subsp. paniculatum essential oil, thus paving the way for the development of effective drugs presenting concomitantly antifungal and anti-inflammatory properties.

Preparation of Ultrafine Polyethylene-Silica Composite Particle with Core-Shell Structure

2012 ◽  
Vol 557-559 ◽  
pp. 554-557 ◽  
Author(s):  
Lei Zu ◽  
Shun Yu Han ◽  
Kai Gu ◽  
Xiu Guo Cui
Keyword(s):  
Shell Structure ◽  
Composite Particle ◽  
Sol Gel ◽  
Core Shell ◽  
Silica Composite ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean ◽  
Core Shell Structure ◽  
Electronic Microscope

A novel ultrafine polyethylene/silica composite particle with core-shell structure was prepared by the sol-gel method in the presence of the melt polyethylene emulsion. A series of samples with different polyethylene content were prepared to investigate the unique characteristics of this original composite particle. The core-shell structure and composition of the composite particle was proved by the transmission electron microscopy observation and Fourier transform infrared spectra. The composite particles possess a spherical morphology and the mean size is about 160nm, presented by the scanning electronic microscope observation and nanoparticle tracking analysis, respectively.

Synthesis of Ce0.8Gd0.05Y0.15O1.9 Nanopowders

2007 ◽  
Vol 336-338 ◽  
pp. 2005-2007
Author(s):  
Xiang Feng Guan ◽  
Zhi Hui Liu ◽  
He Ping Zhou
Keyword(s):  
Electron Microscopy ◽  
Solid Oxide Fuel Cells ◽  
Single Phase ◽  
Fluorite Structure ◽  
Solid Oxide ◽  
Xrd Pattern ◽  
X Ray ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean

Nanosized Ce0.8Gd0.05Y0.15O1.9 powders were synthesized using glycine nitrate process for use as the electrolyte of intermediate temperature solid oxide fuel cells. The powders were characterized by simultaneous thermogravimetry analysis (TGA) and differential thermal analysis (DTA), X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The XRD pattern indicated that the powders exhibited a single phase with cubic fluorite structure. The TEM studies showed that the calcined powders exhibited an almost spherical morphology and the mean size of the particles was 30nm, which is in agreement with the calculated result of XRD.

THE SIZE CONTROL OF UNIFORM NANOCRYSTALLINE Si GRAINS BY CONSTRAINED GROWTH MODEL

2005 ◽  
Vol 19 (15n17) ◽  
pp. 2751-2756 ◽  
Author(s):  
KUNJI CHEN ◽  
KAI CHEN ◽  
PEIGAO HAN ◽  
HECHENG ZOU ◽  
ZHONGYUAN MA ◽  
...  
Keyword(s):  
Size Control ◽  
Nanocrystalline Silicon ◽  
Annealing Conditions ◽  
Crystallization Method ◽  
Transmission Electron ◽  
Mean Size ◽  
Sublayer Thickness ◽  
The Mean ◽  
Size Controlled ◽  
Nanocrystalline Si

Size-controlled nanocrystalline silicon ( nc - Si ) has been prepared from a - SiN x / a - Si:H/a - SiN x ( 'a' standing for amorphous) sandwich structures by thermal annealing. Transmission electron microscope analyses show that the mean size and the grain size distribution (GSD) of the nc - Si are controlled by the annealing conditions and the a - Si sublayer thickness. Based on our theoretical model of constrained crystallization, we interpret the phenomena of the growth halt of nc - Si and higher crystallization temperature for the thinner a - Si sublayers. The experimental results show that constrained crystallization method is promising to achieve uniform and high density nc - Si array which can be used in the future nano-devices.

scholarly journals Модификация структурно-морфологических свойств германия в многослойной нанопериодической структуре Al-=SUB=-2-=/SUB=-O-=SUB=-3-=/SUB=-/Ge с промежуточными слоями Si при отжиге

2021 ◽  
Vol 55 (10) ◽  
pp. 882
Author(s):  
М.В. Байдакова ◽  
Н.А. Берт ◽  
В.Ю. Давыдов ◽  
А.В. Ершов ◽  
А.А. Левин ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Heat Treatment ◽  
Annealing Temperature ◽  
Electron Beam Evaporation ◽  
X Ray Diffraction ◽  
Bimodal Size Distribution ◽  
X Ray ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean

Multi-layered nanosized Al2O3/Ge/Si structures manufactured by electron-beam evaporation and annealed at a temperature within the range 700−900◦C are examined using transmission electron microscopy, Raman spectroscopy and X-ray diffraction techniques. The periodic structure with a good layer planarity is confirmed to retain after heat treatment up to 900◦C. At an annealing temperature above 700◦C, nanocrystallites with a bimodal size distribution start to form within initially amorphous Ge layers, the mean size of small crystallites being determined by Ge layer thickness and annealing temperature. An essential loss of Ge from multi-layered structure after 900oC anneal and development of Ge1−x Six solid solution with x up to 0.07 in the nanocrystallites is revealed.

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