scholarly journals Antioxidant Capacity Assessment of Plant Extracts for Green Synthesis of Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1679
Author(s):  
María Martínez-Cabanas ◽  
Marta López-García ◽  
Pilar Rodríguez-Barro ◽  
Teresa Vilariño ◽  
Pablo Lodeiro ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Metal Nanoparticles ◽  
Metallic Nanoparticles ◽  
Metal Salt ◽  
Radical Scavenging ◽  
Kinetic Studies ◽  
Synthesis Of Nanoparticles ◽  
Transmission Electron ◽  
Scavenging Capacity ◽  
Capacity Method

In this work, water extracts from different bio-based products of plant origin were studied to evaluate their antioxidant capacity and their potential to form metal nanoparticles from aqueous solutions. Two traditional tests, the Folin–Ciocalteu assay and the DPPH radical scavenging capacity method were compared with a more recent one, SNPAC, based on the formation of silver nanoparticles. The silver nanoparticle antioxidant capacity method (SNPAC) was optimized for its application in the characterization of the extracts selected in this work; kinetic studies and extract concentration were also evaluated. The extracts were obtained from leaves of oak, eucalyptus, green tea, white and common thyme, white cedar, mint, rosemary, bay, lemon, and the seaweed Sargassum muticum. The results demonstrate that any of these three methods can be used as a quick test to identify an extract to be employed for nanoparticle formation. Additionally, we studied the synthesis of Cu, Fe, Pb, Ni, and Ag nanoparticles using eucalyptus extracts demonstrating the efficiency of this plant extract to form metallic nanoparticles from aqueous metal salt solutions. Metal nanoparticles were characterized by transmission electron microscopy and dynamic light scattering techniques.

scholarly journals Effects of Fruit Maturity on Physicochemical Properties, Sugar Accumulation and Antioxidant Capacity of Wild Harvested Kakadu Plum (Terminalia ferdinandiana)

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 48
Author(s):  
Anh Dao Thi Phan ◽  
Maral Seidi Damyeh ◽  
Saleha Akter ◽  
Mridusmita Chaliha ◽  
Michael E. Netzel ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Soluble Sugars ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Maturity Stages ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Kakadu Plum ◽  
Terminalia Ferdinandiana

Terminalia ferdinandiana (Kakadu plum), belonging to the family Combretaceae, is endemic to Australia and has a long history of traditional medicinal applications and food cuisine by the Australian Indigenous people. This study investigated the effects of maturity stages on the morphology, physicochemical parameters (total soluble solids (TSS), total acid content (TAC), and pH), soluble sugar profile and antioxidant capacity of Kakadu plum (KP) fruits that were wild harvested from different trees and classified into four different maturity stages (immature to mature). TSS and TAC were determined by standard assays/procedures, main sugars by UHPLC–MS/MS and antioxidant capacity (total phenolic content (TPC) and DPPH free radical scavenging capacity) by spectrophotometry. The results showed that soluble sugars (glucose, sucrose and fructose) ranging from 1.3 to 17.7% dry weight (DW), TSS (17.0–52.7% DW) and TAC (1.3–6.7% DW) increased with maturity. However, antioxidant capacity (TPC in the range of 7.4–21.9% DW and DPPH free radical scavenging capacity from 22 to 76% inhibition at the extract concentration of 20 g·L−1) did not follow the same trend as the one observed for soluble sugars, TSS and TAC. These differences were associated with the tree-to-tree variability as a consequence of the wild harvest condition. This study provides important information to both the KP industry and Indigenous enterprises regarding the selection of the appropriate maturity stage to harvest KP fruit to target for different markets (e.g., low-sugar vs. high-sugar fruit).

scholarly journals Solvent polarity mediates phytochemical yield and antioxidant capacity of Isatis tinctoria

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7857
Author(s):  
Abdul Wakeel ◽  
Sohail Ahmad Jan ◽  
Ikram Ullah ◽  
Zabta Khan Shinwari ◽  
Ming Xu
Keyword(s):  
Free Radical ◽  
Secondary Metabolites ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Solvent Polarity ◽  
Free Radical Scavenging ◽  
Isatis Tinctoria ◽  
Polarity Index ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Secondary metabolites have been extensively used in the treatment of various health problems. The role of solvent polarity on the phytochemical isolation and antioxidant capacity of Isatis tinctoria (woad) is elusive. In the present study, 14 solvents with different polarity were used in the extraction and total phenolic and flavonoid content (TPC and TFC) investigation. Ferricyanide, phosphomolybdenum, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods were used to calculate and compare the antioxidant/free radical scavenging capacity. Our results showed that solvent polarity greatly affects TPC and TFC yield, which is mainly increasing with increasing solvent polarity index and suddenly decreasing at very high polarity. The comparative results showed that TPC is directly correlated with reducing power, antioxidant, and free radical scavenging capacity. Taken together, we conclude that different woad plant parts contain different level of secondary metabolites with a specific polarity that requires a particular solvent with an appropriate polarity index for the extraction. The identification of these biologically active crude extracts and fractions are very important for the basic biological sciences, pharmaceutical applications, and future research for HPLC based active compounds isolation.

scholarly journals Comparison of Functional Properties of the Bael Fruit at Various Levels of Maturity

2020 ◽  
pp. 137-142
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Capacity ◽  
Functional Properties ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Ripe Fruit ◽  
Scavenging Capacity ◽  
Anti Inflammatory ◽  
Hydroxyl Hydrogen

Aegle marmelos fruit is packed with immense medicinal properties. The main objective of the studywas to assess and compare the phytochemical potency, antioxidant capacity and anti-inflammatory activity of unripe, ripe and overripe fruits. The fruit pulp was dried and extract (ripe fruit) was prepared in various solvents. Phytochemical assessment was done and the solvent which exhibited maximum potency was chosen as unripe and ripe fruit solvents. Phytochemical assessment, estimation of phenols (FolinCiocalteau method), antioxidant capacity (Molybdate method), anti-inflammatory activity (membrane stabilization property method) and Free radical scavenging capacity for hydroxyl, hydrogen peroxide and DPPH free radicals were carried out. The phytochemical screening of the ripe fruit showed maximum intensity in hydroethanolic extract. On comparison, ripe fruit extract possessed a greater phytochemical potency, antioxidant capacity and inhibition against hydroxyl, hydrogen peroxide, DPPH radicals. The total phenolic content of the over ripe fruit was high. The anti-inflammatory activity of unripe fruit was slightly higher than ripe fruit. This signifies that phytochemical potency and functional properties of the fruit differs with maturity stages.

First Report on Better Functional Property of Black Chicken Meat from India

2021 ◽  
Author(s):  
Renuka Sehrawat ◽  
Rekha Sharma ◽  
Sonika Ahlawat ◽  
Vivek Sharma ◽  
Mohan Singh Thakur ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Breast Meat ◽  
Vitro Assay ◽  
Antioxidant Power ◽  
Meat Extract ◽  
Scavenging Capacity

Background: Indian domesticated chickens have a wide variety of phenotypes. Unlike other chicken breeds, Kadaknath chicken has black meat and is used by indigenous tribal people for its invigorating and therapeutic properties. To look for the functional traits that might be contributing towards the acclaimed benefits, free radical scavenging capacity and metal chelating ability of Kadaknath meat were explored for the first time in comparison to the commercial Cobb broiler. Methods: During the period 2018-2020, breast and thigh meat were collected from chickens (n=20/ group) at their commercial slaughter age. Meat extract was used for qualitative evaluation of protein as well as the antioxidant capacity utilizing diverse in vitro methods corresponding to different antioxidation mechanisms.Result: Protein concentration (g/100g of tissue) in Kadaknath breast (25.25 ± 0.31) and thigh (19.98 ± 0.29) meat was significantly (P less than 0.05) higher than the Cobb. Breast meat had better scavenging capacity than the thigh. The superiority of the antioxidant capacity of Kadaknath meat was explicitly established by more than one in vitro assay. Free radical scavenging assays viz. 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS); 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Cupric reducing antioxidant capacity (CUPRAC) had significantly (P less than 0.05) higher values for Kadaknath in comparison to the Cobb broiler meat. Ferric reducing antioxidant power (FRAP) values (mM Fe2+/g of tissue) were also more in breast meat of Kadaknath (26.97±0.37) than the Cobb (15.24±0.40). Similarly, FRAP values were higher in Kadaknath (33.85±0.47) than the Cobb (19.2±0.31) thigh meat. Kadaknath had higher antioxidant capacity as reflected by metal chelation inhibition value. These findings help to explain the unique nutritional and functional characteristics of Kadaknath black-bone chicken and provide basic research data for exploring the commercial potential of its meat in the fields of functional foods, cosmeceuticals and nuutraceuticals.

scholarly journals Comparison of Radical Scavenging Capacities of Eleven Sargassum Species Grown off the Korean Coast (P06-082-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Su-Hyeon Baek ◽  
Seung-Jin Jeong ◽  
Chai-Hyeon Lee ◽  
Hyeung-Rak Kim ◽  
Bohkyung Kim ◽  
...  
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Antioxidant Capacities ◽  
Scavenging Capacity ◽  
Radical Generation ◽  
Dpph Radical Scavenging

Abstract Objectives Sargassum (S) species, a marine brown alga consumed in Asian countries, have various health benefits, such as improving the conditions of cardiovascular disease, osteoarthritis, and hypopigmentation. And these benefits are associated with their strong antioxidant capacities. However, antioxidant capacities of S. species have not been thoroughly explored and compared. Therefore, the purpose of this study was to compare the antioxidant capacities of eleven S. species grown off the Korean coast. Methods Ethanol extracts of eleven S. Species (S. hemiphyllum, S. coreanum, S. horneri, S. Confusum, S. siliquastrum, S. miyabei, S. Serratifolium, S. filicinum, S. Micracanthum, S. macrocarpum, and S. yendoi) provided by National Marine Biodiversity Institute of Korea were evaporated. The powdered samples were dissolved in 80% methanol as 100 mg/L to measure ABTS, DPPH, and superoxide radical scavenging activities. For the ABTS and DPPH radical scavenging capacities, the antioxidant capacities were expressed as mg vitamin C equivalent antioxidant capacity (VCEAC)/100 mg. For the superoxide radical scavenging capacity, inhibition rate of superoxide radical generation (%) was calculated. Results Among eleven species, four showed notable radical scavenging capacities including S. miyabei, S. hemiphyllum, S. filicinum, and S. serratifolium. For DPPH radical scavenging capacity, S. miyabei showed the greatest antioxidant capacity (193.6 mg VCEAC/100 mg) followed by S. hemiphyllum, (137.9), S. filicinum (78.2), and S. serratifolium (75.4). For ABTS radical scavenging capacity, S. hemiphyllum showed the greatest capacity as 205.7 mg VCEAC/100 mg followed by S. miyabei (186.2), S. filicinum (140.1), and S. serratifolium (99.5). S. hemiphyllum inhibited about 52% of superoxide radical generation followed by S. miyabei (43.7%), S. filicinum (40.4%) and S. serratifolium (34.8%). Vitamin C as positive control inhibited 34.7% of superoxide radical generation. Conclusions Our results exhibited that various S. species showed different antioxidant capacities, indicating the different compositions and proportions of antioxidant compounds in the species. Therefore, further study is warranted to investigate bioactive compounds of the S. species based on our screening. Funding Sources This research was supported by the Pukyong National University Research Fund (2018).

Redox Behavior and Radical Scavenging Capacity of Hepatoprotective Nutraceutical Preparations

2021 ◽  
Vol 02 ◽  
Author(s):  
Douglas Vieira Thomaz ◽  
Pierre Alexandre dos Santos
Keyword(s):  
Physical Chemistry ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Similar Response ◽  
Redox Behavior ◽  
Herbal Extracts ◽  
Over The Counter ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Oxidation Of Methionine

Background: Over-the-counter hepatoprotective nutraceuticals are highly commercialized preparations worldwide. However, their alleged antioxidant capacity and health benefits are still not fully understood. Objective: This work showcased the first investigation of the redox behavior of hepatoprotective nutraceuticals by spectrophotometric and electrochemical approaches. Method: The samples were segregated into two groups, namely: A, B, and C based on isolated compounds (IC); and D, E, and F based on standardized herbal extracts (SHE). Results: Results evidenced that IC showcase similar response and distinctions could be attributed to varying concentrations of choline. In SHE, the slopes showcased superimposition due to the presence of Peumus boldus. The electrochemical assays showcased that samples A and C exhibited a single anodic peak at Ep1a ≈ +0.7 V, which could be attributed to the oxidation of methionine; while samples D, E and F, showcased two anodic peaks at Ep1a ≈ +0.35V and Ep2a ≈ +0.7 V, suggesting the oxidation of phenolic and amine moieties respectively. Furthermore, the first two principal components explained 84.8% of all variance in the model, thereby suggesting statistical reproducibility. Conclusion: This work showcased the first investigation of the redox behavior of hepatoprotective nutraceuticals, thereby shedding light on their antioxidant capacity and physical chemistry.

The emerging roles of arthropods and their metabolites in the green synthesis of metallic nanoparticles

2016 ◽  
Vol 5 (6) ◽  
Author(s):  
Agbaje Lateef ◽  
Sunday A. Ojo ◽  
Joseph A. Elegbede
Keyword(s):  
Cost Effectiveness ◽  
Green Synthesis ◽  
Metal Nanoparticles ◽  
Metallic Nanoparticles ◽  
Animal Species ◽  
Chemical Methods ◽  
Synthesis Of Nanoparticles ◽  
Safe Handling ◽  
Nanoscience And Nanotechnology ◽  
Physical And Chemical

AbstractNanotechnology has remained relevant as a multifacet discipline, which cuts across different areas of science and technology. Several successful attempts had been documented regarding the involvement of biological materials in the green synthesis of various metal nanoparticles (MeNPs) because of their eco-friendliness, cost-effectiveness, safe handling, and ultimately less toxicity as opposed to the physical and chemical methods with their concomitant problems. Biological agents, including bacteria, fungi, algae, enzymes, plants, and their extracts, have been implicated in most cases by several authors. Moreover, nanotechnology in recent times has also made an inroad for animal species, specifically arthropods and metabolites thereof to be used as excellent candidates for the green synthesis of MeNPs. The increasing literature on the use of metabolites of arthropods for the green synthesis of nanoparticles has necessitated the need to document a review on their relevance in nanobiotechnology. The review, which represents the first of its kind, seeks to underscore the importance of arthropods in the multidisciplinary subject of nanoscience and nanotechnology.

scholarly journals Antioxidants: Classification, Natural Sources, Activity/Capacity Measurements, and Usefulness for the Synthesis of Nanoparticles

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4135
Author(s):  
Jolanta Flieger ◽  
Wojciech Flieger ◽  
Jacek Baj ◽  
Ryszard Maciejewski
Keyword(s):  
Antioxidant Activity ◽  
Green Synthesis ◽  
Antioxidant Capacity ◽  
Metallic Nanoparticles ◽  
Food Additives ◽  
Free Radical Damage ◽  
Synthesis Of Nanoparticles ◽  
Natural Extracts ◽  
Reducing Substances

Natural extracts are the source of many antioxidant substances. They have proven useful not only as supplements preventing diseases caused by oxidative stress and food additives preventing oxidation but also as system components for the production of metallic nanoparticles by the so-called green synthesis. This is important given the drastically increased demand for nanomaterials in biomedical fields. The source of ecological technology for producing nanoparticles can be plants or microorganisms (yeast, algae, cyanobacteria, fungi, and bacteria). This review presents recently published research on the green synthesis of nanoparticles. The conditions of biosynthesis and possible mechanisms of nanoparticle formation with the participation of bacteria are presented. The potential of natural extracts for biogenic synthesis depends on the content of reducing substances. The assessment of the antioxidant activity of extracts as multicomponent mixtures is still a challenge for analytical chemistry. There is still no universal test for measuring total antioxidant capacity (TAC). There are many in vitro chemical tests that quantify the antioxidant scavenging activity of free radicals and their ability to chelate metals and that reduce free radical damage. This paper presents the classification of antioxidants and non-enzymatic methods of testing antioxidant capacity in vitro, with particular emphasis on methods based on nanoparticles. Examples of recent studies on the antioxidant activity of natural extracts obtained from different species such as plants, fungi, bacteria, algae, lichens, actinomycetes were collected, giving evaluation methods, reference antioxidants, and details on the preparation of extracts.

Hydrazine Based Facile Synthesis and Ordered Assembly of Metal Nanoparticles (Au, Ag) on a Bacterial Surface Layer Protein Template

2008 ◽  
Vol 8 (7) ◽  
pp. 3565-3569 ◽  
Author(s):  
Sarang S. Puranik ◽  
Hrushikesh M. Joshi ◽  
S. B. Ogale ◽  
K. M. Paknikar
Keyword(s):  
Silver Nanoparticles ◽  
Metal Nanoparticles ◽  
Metal Salt ◽  
Chemical Reduction ◽  
Average Diameter ◽  
Protein Array ◽  
Gold And Silver Nanoparticles ◽  
Bacterial Surface ◽  
Transmission Electron ◽  
Ordered Assembly

An efficient and facile procedure is developed for concurrent in situ synthesis and ordered assembly of metal nanoparticles on a periodic two dimensional protein array. The S-layer protein of Bacillus subtilis exhibiting uniform pore size is used as template. Synthesis of gold and silver nanoparticles anchoring on the pores of S-layer is achieved by chemical reduction of respective metal salt laden protein template. Transmission electron microscopy reveals formation of well ordered and separated gold and silver nanoparticles with an average diameter of 6 ± 1 nm and 4 ± 1 nm, respectively. The periodic arrangement of nanoparticles is dictated by the native structure of S-layer protein array as the nanoparticle locations are found to be correlated to the nanosized pores of the crystalline S-layer array.

scholarly journals Synthesis and Catalytic and Biological Activities of Silver and Copper Nanoparticles Using Cassia occidentalis

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Manjul Gondwal ◽  
Geeta Joshi nee Pant
Keyword(s):  
Electron Microscopy ◽  
Metal Nanoparticles ◽  
Copper Nanoparticles ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Salmonella Typhi ◽  
Cassia Occidentalis ◽  
X Ray ◽  
Transmission Electron

An ecofriendly and green method for the synthesis of silver and copper nanoparticles has developed using aqueous leaves extract of Cassia occidentalis. The formation of AgNPs and CuNPs was monitored by measuring the UV-Vis spectra. The morphology and crystalline phase of the metal nanoparticles were determined using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) with X-ray energy dispersive spectrophotometer (EDX) and X-ray diffraction (XRD). The synthesized metal nanoparticles were generally found to be spherical and oval in shape. The AgNPs and CuNPs showed highly potent antibacterial activity against Escherichia coli and Salmonella typhi bacteria, respectively. The CuNPs showed higher radical scavenging activity than AgNPs. The AgNPs showed lower haemolysis (1.7%) exhibiting lesser toxicity as compared to CuNPs. The CuNPs have better catalytic ability for the reduction of 4-nitrophenol and 2-nitrophenol as compared to AgNPs.

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