scholarly journals Comparison of Functional Properties of the Bael Fruit at Various Levels of Maturity

2020 ◽  
pp. 137-142
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Capacity ◽  
Functional Properties ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Ripe Fruit ◽  
Scavenging Capacity ◽  
Anti Inflammatory ◽  
Hydroxyl Hydrogen

Aegle marmelos fruit is packed with immense medicinal properties. The main objective of the studywas to assess and compare the phytochemical potency, antioxidant capacity and anti-inflammatory activity of unripe, ripe and overripe fruits. The fruit pulp was dried and extract (ripe fruit) was prepared in various solvents. Phytochemical assessment was done and the solvent which exhibited maximum potency was chosen as unripe and ripe fruit solvents. Phytochemical assessment, estimation of phenols (FolinCiocalteau method), antioxidant capacity (Molybdate method), anti-inflammatory activity (membrane stabilization property method) and Free radical scavenging capacity for hydroxyl, hydrogen peroxide and DPPH free radicals were carried out. The phytochemical screening of the ripe fruit showed maximum intensity in hydroethanolic extract. On comparison, ripe fruit extract possessed a greater phytochemical potency, antioxidant capacity and inhibition against hydroxyl, hydrogen peroxide, DPPH radicals. The total phenolic content of the over ripe fruit was high. The anti-inflammatory activity of unripe fruit was slightly higher than ripe fruit. This signifies that phytochemical potency and functional properties of the fruit differs with maturity stages.

scholarly journals Constituents of French Marigold (Tagetes patulaL.) Flowers Protect Jurkat T-Cells against Oxidative Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Irakli Chkhikvishvili ◽  
Tamar Sanikidze ◽  
Nunu Gogia ◽  
Maia Enukidze ◽  
Marine Machavariani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
T Cells ◽  
Radical Scavenging ◽  
Jurkat Cells ◽  
Cellular Mechanisms ◽  
Jurkat T Cells ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Inflammatory

The flowers of French marigold (Tagetes patulaL.) are widely used in folk medicine, in particular for treating inflammation-related disorders. However, cellular mechanisms of this activity demand further investigation. In the present work, we studied the potential ofT. patulacompounds to alleviate the oxidative stress in hydrogen peroxide-challenged human lymphoblastoid Jurkat T-cells. Crude extracts of marigold flowers and purified fractions containing flavonoids patuletin, quercetagetin, and quercetin and their derivatives, as well as the carotenoid lutein, were brought in contact with Jurkat cells challenged with 25 or 50 μM H2O2. Hydrogen peroxide caused oxidative stress in the cells, manifested as generation of superoxide and peroxyl radicals, reduced viability, arrested cell cycle, and enhanced apoptosis. The stress was alleviated by marigold ingredients that demonstrated high radical-scavenging capacity and enhanced the activity of antioxidant enzymes involved in neutralization of reactive oxygen species. Flavonoid fraction rich in quercetin and quercetagetin showed the highest cytoprotective activity, while patuletin in high dose exerted a cytotoxic effect associated with its anticancer potential.T. patulacompounds enhanced the production of anti-inflammatory and antioxidant interleukin-10 (IL-10) in Jurkat cells. Both direct radical-scavenging capacity and stimulation of protective cellular mechanisms can underlay the anti-inflammatory properties of marigold flowers.

scholarly journals Antioxidant Capacity of “Mexican Arnica”Heterotheca inuloidesCass Natural Products and Some Derivatives: Their Anti-Inflammatory Evaluation and Effect onC. elegansLife Span

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
José Luis Rodríguez-Chávez ◽  
Elvia Coballase-Urrutia ◽  
Antonio Nieto-Camacho ◽  
Guillermo Delgado-Lamas
Keyword(s):  
Life Span ◽  
Antioxidant Capacity ◽  
Harmful Effect ◽  
Inflammatory Activity ◽  
Biological Processes ◽  
Ros Scavenging ◽  
C Elegans ◽  
Scavenging Capacity ◽  
Anti Inflammatory ◽  
Stable Free Radicals

It has been suggested that the accumulation of biomolecular damage caused by reactive oxygen species (ROS) contributes to aging. The antioxidant activity is related to the ability of certain compounds to protect against the potentially harmful effect of processes or reactions involving ROS. This ability is associated with the termination of free radical propagation in biological systems. FromHeterotheca inuloidesvarious compounds which have shown to possess antioxidant capacity and scavenging ROS. The aim of this study was to determine the antioxidant capacity of additional natural components isolated fromH. inuloidesand some semisynthetic derivatives, their anti-inflammatory activity and the effect onCaenorhabditis elegansnematode life span. Compounds showed ability to inhibit various biological processes such as lipid peroxidation, scavenge nonbiological important oxidants such as1O2,OH∙, H2O2, and HOCl and scavenge non biological stable free radicals (DPPH). Some cadinane type compounds showed possess antioxidant, ROS scavenging capacity, anti-inflammatory activity, and effect on theC. eleganslife span. Flavonoid type compounds increased the life of the nematode and quercetin was identified as the compound with the greatest activity. The modification of chemical structure led to a change in the antioxidant capacity, the anti-inflammatory activity, and the survival of the worm.

scholarly journals Characterization and Uptake of Strawberry-Derived Exosome-Like Nanovesicles by Human Aortic Endothelial Cells

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 310-310
Author(s):  
Jessica Danh ◽  
Brandon Canup ◽  
Rami Najjar ◽  
Maureen Meister ◽  
Hamed Laroui ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Aortic Endothelial Cells ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Human Aortic Endothelial Cells ◽  
Dpph Radical Scavenging

Abstract Objectives To characterize strawberry (SB)-derived exosome-like nanovesicles (ELNs), assess the total phenolic content, total flavonoid content, and total antioxidant capacity as well as its uptake by human aortic endothelial cells (HAECs). Methods SB ELNs were extracted using differential centrifugation. After final ultracentrifugation at 100,000 × g for 1 h, pellets were collected and washed in PBS. Characterization was performed using dynamic light scattering measurements. Total phenolic content (TPC) and flavonoid content (TFC) were determined using Folin-Ciocalteu and aluminum chloride, respectively. Antioxidant capacity was determined using the Trolox equivalent antioxidant capacity (TEAC) and ferric reducing ability of plasma (FRAP) assay while free radical scavenging power was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay. Cell viability and uptake were assessed in HAECs. Cell viability was measured after 24-h incubation with SB ELNs using MTT reagent. Cell uptake was measured after 12-h incubation with 100 μg/mL coumarin-6 (C-6) labelled SB ELNs. Fluorescent microscopy and flow cytometry were used to detect cellular uptake of C-6 labelled SB ELNs on a LSR II. Results SB ELNs were sized at 119.4 ± 28.3 nm (PDI = 0.29 ± 0.06). TPC and TFC of SB ELNs were 158.9 ± 22.6 μmol GAE/L and 5.1 ± 0.4 μg QE/mL, respectively. Antioxidant capacity of SB ELNs was 211.38 ± 6.3 μmol TE/L and 118.0 ± 7.6 μmol Fe2+/L by TEAC and FRAP, respectively. DPPH radical scavenging capacity was 181.3 ± 2.5 μmol TE/L in SB ELNs. No cytotoxic effects were observed for SB ELNs in HAECs. Uptake of SB ELNs by HAECs was 15.3% higher compared to baseline levels. Conclusions We report, for the first time, the presence of phenolics and flavonoids in the cargo of SB ELNs, SB ELNs antioxidant capacity, and demonstrate their uptake by HAECs. Taken together, these findings support the need to further characterize and explore the antioxidant potential of SB ELNs in vitro and in vivo. Funding Sources None.

scholarly journals Antioxidation and Tyrosinase Inhibitory Ability of Coffee Pulp Extract by Ethanol

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Cheng-You Chen ◽  
Chia-Hung Shih ◽  
Tzu-Che Lin ◽  
Ji-Hong Zheng ◽  
Chien-Chieh Hsu ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Economic Value ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Tyrosinase Inhibition ◽  
Coffee Pulp ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

Background. Following petroleum, coffee is the second most commonly traded commodity globally. It is also a popular good with economic value, as well as value in terms of leisure and culture. However, coffee processing generates a large amount of waste, resulting in environmental concerns. Therefore, in this study, ethanol was used to extract coffee waste (coffee pulp). High-performance liquid chromatography was conducted to examine the caffeine content and chlorogenic acid content, and the antioxidant capacity (i.e., the total phenolic content, total flavonoid content, DPPH-free radical scavenging capacity, ABTS-free radical scavenging capacity, and reductive capacity) and the tyrosinase inhibition capacity of the coffee pulp extracted using ethanol were comprehensively evaluated. Results. The results showed that the coffee pulp extract obtained using 70% ethanol had the highest tyrosinase inhibition capacity, whereas that obtained using 50% ethanol had the most satisfactory antioxidant capacity (total phenolic content, total flavonoid content, DPPH-free radical scavenging capacity, ABTS-free radical scavenging capacity, and reductive capacity). Conclusion. The results revealed that coffee pulp has superior antioxidant capacity and tyrosinase inhibition capacity when extracted by ethanol. Increasing the economic value of coffee pulp can solve the environmental concerns caused by coffee waste.

scholarly journals Anti-Inflammatory and Antioxidant Activities ofSalvia fruticosa: An HPLC Determination of Phenolic Contents

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Rima Boukhary ◽  
Karim Raafat ◽  
Asser I. Ghoneim ◽  
Maha Aboul-Ela ◽  
Abdalla El-Lakany
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Aerial Parts ◽  
Phenolic Constituents ◽  
Anti Inflammatory

Objectives.Salvia fruticosaMill. (S. fruticosa) is widely used in folk medicine. Accordingly, the present study was designed to evaluate the antioxidant and anti-inflammatory activities ofS. fruticosa, and to determine the phenolic constituents of its extracts.Methods. The antioxidant activity was determined using 2,2-diphenylpicrylhydrazyl assay. Total phenolic contents were estimated using Folin-Ciocalteu reagent, and high-performance liquid chromatography was performed to identify phenolic constituents. To evaluate the anti-inflammatory activity, carrageenan-induced mouse paw edema was determined plethysmographically.Key Findings. Different plant extracts demonstrated strong radical scavenging activity, where the ethyl acetate extract had the highest value in the roots and the lowest in the aerial parts. This antioxidant activity was correlated to the total phenolic content of different extracts, where rutin and luteolin were the most abundant constituents. Interestingly, both the roots and aerial parts revealed a significant anti-inflammatory activity comparable to diclofenac.Conclusions. This study is the first to demonstrate pharmacologic evidence of the potential anti-inflammatory activity ofS. fruticosa. This activity may partly be due to the radical scavenging effects of its polyphenolic contents. These findings warrant the popular use of the East Mediterranean sage and highlight the potential of its active constituents in the development of new anti-inflammatory drugs.

scholarly journals Investigating the Antioxidant Characteristics of Saccharina Latissima and Its Ability to Reduce Inflammation in RAW 264.7 Cells (P06-091-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Abigail Wiegand ◽  
Gretchen Grebe ◽  
Carrie Byron ◽  
Angela Myracle
Keyword(s):  
Gallic Acid ◽  
Radical Scavenging ◽  
Saccharina Latissima ◽  
Total Phenolic ◽  
Raw 264.7 ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Inflammatory ◽  
The University ◽  
Gallic Acid Equivalent

Abstract Objectives The goal of this study is to understand the anti-inflammatory characteristics of Saccharina latissima (sugar kelp). Sugar kelp is a brown macroalga that grows readily off the coast of Maine. Other seaweeds are known to contain bioactive chemicals that reduce inflammation—a contributing factor to metabolic syndrome and type 2 diabetes. It is hypothesized that injuring farmed sugar kelp by trimming the kelp may increase the beneficial bioactives and promote anti-inflammatory effects. The specific aims are (1) to produce extracts of Maine-grown sugar kelp through accelerated solvent extraction (ASE), (2) to measure the samples’ antioxidant capacity, and (3) to investigate the samples’ anti-inflammatory effect in vitro. Methods Sugar kelp samples were freeze-dried and extracts were produced using ASE. Total phenolic content was measured using the Folin-Ciocalteau assay, and radical scavenging capacity was measured with a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The sugar kelp extracts were tested for cytotoxicity in RAW 264.7 macrophages using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to induce inflammation in the cells, and the attenuation effect of extract doses ranging from 12.5–50 µg total phenolics (TP)/mL was observed by measuring nitric oxide (NO) secretion with the Griess assay. Results Preliminary data shows that sugar kelp contains polyphenols (8.4–16.0 mg gallic acid equivalent/g dried biomass) and radical scavenging capacity (IC50 = 61.8–79.5 µg/mL). The data did not demonstrate the trimming the kelp has a beneficial effect to increase phenolic compounds. The polyphenolic contents of the trimmed samples were lower than the untrimmed (9.5 vs. 13.1 mg gallic acid equivalent/g dried biomass). The radical scavenging capacity decreased when the samples were trimmed (14.7%). The greatest decrease in NO production was at a concentration of 50 μg TP/mL for a trimmed sample (up to 78.5%), which supports the hypothesis. Conclusions This data suggests that sugar kelp may be a good source of antioxidants and trimmed samples may contain additional bioactives to help decrease inflammation. Further investigation is needed to confirm the hypothesis. Funding Sources The University of Maine The University of New England

scholarly journals Assessment of Antioxidant Capacity and Phytochemical Composition of Brown and Red Seaweeds Sampled off Red Sea Coast

2021 ◽  
Vol 11 (23) ◽  
pp. 11079
Author(s):  
Abla A. M. Farghl ◽  
Zaki M. Al-Hasawi ◽  
Mostafa M. El-Sheekh
Keyword(s):  
Amino Acids ◽  
Antioxidant Capacity ◽  
Essential Fatty Acids ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Total Phenolic ◽  
Red Seaweeds ◽  
Scavenging Capacity ◽  
Total Antioxidant

Seaweeds are becoming a viable source of biologically active composites with a hopeful application as nutraceuticals, functional food components, and medicinal agents. In the present study, the antioxidant capacity and biochemical compositions of four seaweeds; Polycladia indica and Turbinaria ornata (Phaeophyceae) and Laurencia obtusa and Sarconema scinaioides (Rhodophyceae), were estimated. The results indicated that T. ornata showed the maximum value of total phenolic compound (TPC), flavonoid content, β-carotene, carbohydrate and has maximum percentage of DPPH radical scavenging capacity, total antioxidant capacity (TAC), and total reducing capacity (TRC) (72.48%, 15.02%, and 53.24% inhibition, respectively), while the highest contents of ascorbic acid, lipid, calcium, and zinc were observed in L. obtusa. P. indica showed the highest protein contents, dietary fibers, sodium, potassium, magnesium, and total amino acids. Glutamic, aspartic, proline, and methionine were the most frequent amino acids in the four selected seaweeds. Brown seaweeds (T. ornata and P. indica) attained the highest percent of the total polyunsaturated (ω6 and ω3) essential fatty acids. The biochemical content of these seaweed species, as well as their antioxidant properties, make them interesting candidates for nutritional, pharmacological, and therapeutic applications.

scholarly journals Extraction, Phenolic Content and Hydrogen Peroxide Scavenging Capacity of Extracts from Some Honey Samples, Propolis and Bee Pollen

2021 ◽  
Vol 10 (1) ◽  
pp. 27-32
Author(s):  
Zohra Mohammedi
Keyword(s):  
Hydrogen Peroxide ◽  
Radical Scavenging Activity ◽  
Local Population ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Natural Food ◽  
Scavenging Activity ◽  
Bee Pollen ◽  
Scavenging Capacity ◽  
Phenolic Extracts

Honey and propolis is natural food, produced by honey bees (Apis mellifera) and largely used by the local population for its medicinal properties. Our work aims to extract and evaluate the hydrogen peroxide scavenging capacity of different phenolic extracts from some bee products. Phenolic compounds from honey samples, propolis, and bee pollen were extracted by methanol and subjected to radical scavenging activity towards hydrogen peroxide. The results showed the highest values for the total phenolic and total flavonoïd contents in propolis and bee pollen, and a great hydrogen peroxide (H2O2) inhibition (IC50: 0.205 - 2.210 µg/mL) with honey extracts, while sample “multiflower” is the better antioxidant, more than ascorbic acid used as control. The less scavenging activity was observed with the extract from bee pollen (IC50: 39.383 µg/mL). To combat the harmful effects of free radicals, especially reactive oxygen species including hydrogen peroxide, it is important to use phenolic extracts instead of using honey as it is, and extracts from the honey of different types are excellent antioxidants compared to other bee products.

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