scholarly journals Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method

Nanomaterials ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 887 ◽  
Author(s):  
Jakob Blomgren ◽  
Fredrik Ahrentorp ◽  
Dag Ilver ◽  
Christian Jonasson ◽  
Sobhan Sepehri ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Nanoparticle ◽  
Limit Of Detection ◽  
Measurement Techniques ◽  
Magnetic Signal ◽  
Rolling Circle ◽  
Induction Technique ◽  
Data Points ◽  
Balanced Detection ◽  
Complete Frequency

We developed a novel biodetection method for influenza virus based on AC magnetic susceptibility measurement techniques (the DynoMag induction technique) together with functionalized multi-core magnetic nanoparticles. The sample consisting of an incubated mixture of magnetic nanoparticles and rolling circle amplified DNA coils is injected into a tube by a peristaltic pump. The sample is moved as a plug to the two well-balanced detection coils and the dynamic magnetic moment in each position is read over a range of excitation frequencies. The time for making a complete frequency sweep over the relaxation peak is about 5 minutes (10 Hz–10 kHz with 20 data points). The obtained standard deviation of the magnetic signal at the relaxation frequency (around 100 Hz) is equal to about 10−5 (volume susceptibility SI units), which is in the same range obtained with the DynoMag system. The limit of detection with this method is found to be in the range of 1 pM.

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

scholarly journals Simultaneous Determination of Penicillin G and Chloramphenicol in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay

2020 ◽  
Vol 14 (1) ◽  
pp. 59-69
Author(s):  
Milka Atanasova ◽  
Yavor Ivanov ◽  
Elena Zvereva ◽  
Anatoly Zherdev ◽  
Tzonka Godjevargova
Keyword(s):  
Magnetic Nanoparticles ◽  
Simultaneous Determination ◽  
Magnetic Nanoparticle ◽  
Limit Of Detection ◽  
Animal Health ◽  
Simultaneous Detection ◽  
Penicillin G ◽  
Frequent Use ◽  
Simultaneous Immunoassay

Background: Antibiotic residues are a problem of increasing importance and have direct consequences for human and animal health. The frequent use of antibiotics in veterinary practice causes their excretion in milk in dairy cattle. This way, they can easily enter the human body through the consumption of milk and dairy products. Objectives: This induces the need for accurate and sensitive methods to monitor antibiotic levels in milk. The aim of this study was to develop a rapid and sensitive magnetic nanoparticle-based fluorescence immunoassay for the simultaneous detection of chloramphenicol and penicillin G in milk. Methods: Magnetic nanoparticles were synthesized and functionalized with (3-aminopropyl)triethoxysilane. Chloramphenicol-Ovalbumin and Chloramphenicol-Ovalbumin-Fluorescein-5-isothiocyanate conjugates were prepared. Penicillin G – ATTO 633 fluorescent conjugate was synthesized. Antibodies against chloramphenicol and penicillin G were immobilized onto the magnetic nanoparticles. The competitive fluorescent immunoassay was developed. The optimal concentration of the antibody-magnetic nanoparticles and the fluorescent conjugates for the assay was determined. The calibration curves for the antibiotics in buffer and milk were plotted. Fluorescent immunoassay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed. Results: The limit of detection by the simultaneous immunoassay of chloramphenicol and penicillin G in milk was 0.85 ng/mL and 1.6 ng/mL, respectively. The recovery of different concentrations of chloramphenicol and penicillin G in milk samples varied from 98% to 106%. Conclusions: A rapid and sensitive magnetic nanoparticle-based immunofluorescent assay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed. The magnetic nanoparticles ensured rapid and easy procedure.

scholarly journals Understanding intracellular nanoparticle trafficking fates through spatiotemporally resolved magnetic nanoparticle recovery

2021 ◽  
Author(s):  
Emily Sheridan ◽  
Silvia Vercellino ◽  
Lorenzo Cursi ◽  
Laurent Adumeau ◽  
James A. Behan ◽  
...  
Keyword(s):  
Decision Making ◽  
Magnetic Nanoparticles ◽  
Magnetic Nanoparticle ◽  
Magnetic Extraction

We describe how magnetic nanoparticles can be used to study intracellular nanoparticle trafficking, and how magnetic extraction may be integrated with downstream analyses to investigate nanoscale decision-making events.

scholarly journals Therapeutic Potential of Magnetic Nanoparticle-Based Human Adipose-Derived Stem Cells in a Mouse Model of Parkinson’s Disease

2021 ◽  
Vol 22 (2) ◽  
pp. 654
Author(s):  
Ka Young Kim ◽  
Keun-A Chang
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Magnetic Nanoparticles ◽  
Substantia Nigra ◽  
Mouse Model ◽  
Magnetic Nanoparticle ◽  
Imaging System ◽  
Therapeutic Potential ◽  
Adipose Derived Stem Cells

Parkinson’s disease (PD) is a progressive neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra. Several treatments for PD have focused on the management of physical symptoms using dopaminergic agents. However, these treatments induce various adverse effects, including hallucinations and cognitive impairment, owing to non-targeted brain delivery, while alleviating motor symptoms. Furthermore, these therapies are not considered ultimate cures owing to limited brain self-repair and regeneration abilities. In the present study, we aimed to investigate the therapeutic potential of human adipose-derived stem cells (hASCs) using magnetic nanoparticles in a 6-hydroxydopamine (6-OHDA)-induced PD mouse model. We used the Maestro imaging system and magnetic resonance imaging (MRI) for in vivo tracking after transplantation of magnetic nanoparticle-loaded hASCs to the PD mouse model. The Maestro imaging system revealed strong hASCs signals in the brains of PD model mice. In particular, MRI revealed hASCs distribution in the substantia nigra of hASCs-injected PD mice. Behavioral evaluations, including apomorphine-induced rotation and rotarod performance, were significantly recovered in hASCs-injected 6-OHDA induced PD mice when compared with saline-treated counterparts. Herein, we investigated whether hASCs transplantation using magnetic nanoparticles recovered motor functions through targeted brain distribution in a 6-OHDA induced PD mice. These results indicate that magnetic nanoparticle-based hASCs transplantation could be a potential therapeutic strategy in PD.

scholarly journals Phthalocyanine-Functionalized Magnetic Silica Nanoparticles as Anion Chemosensors

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1632
Author(s):  
João M. M. Rodrigues ◽  
Andreia S. F. Farinha ◽  
Zhi Lin ◽  
José A. S. Cavaleiro ◽  
Augusto C. Tome ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Dimethyl Sulfoxide ◽  
Silica Nanoparticles ◽  
Hybrid Material ◽  
Limit Of Detection ◽  
Active Material ◽  
Anion Detection ◽  
Anionic Species ◽  
Magnetic Silica ◽  
Magnetic Silica Nanoparticles

Anionic species are one of the most common pollutants in residual and freshwaters. The presence of anthropogenic anions in water drastically increases the toxicity to living beings. Here, we report the preparation of a new optical active material based on tri(tosylamino)phthalocyanines grafted to ferromagnetic silica nanoparticles for anion detection and removal. The new unsymmetrical phthalocyanines (Pcs) proved to be excellent chemosensors for several anions (AcO−, Br−, Cl−, CN−, F−, H2PO4−, HSO4−, NO2−, NO3−, and OH−) in dimethyl sulfoxide (DMSO). Furthermore, the Pcs were grafted onto magnetic nanoparticles. The resulting novel hybrid material showed selectivity and sensitivity towards CN−, F−, and OH− anions in DMSO with limit of detection (LoD) of ≈4.0 µM. In water, the new hybrid chemosensor demonstrated selectivity and sensitivity for CN− and OH− anions with LoD of ≈0.2 µM. The new hybrids are easily recovered using a magnet, allowing recyclability and reusability, after acidic treatment, without losing the sensing proprieties.

Simulated Clustering Dynamics of Colloidal Magnetic Nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Frederik Laust Durhuus ◽  
Lau Halkier Wandall ◽  
Mathias Hoeg Boisen ◽  
Mathias Kure ◽  
Marco Beleggia ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Self Assembly ◽  
Biomedical Applications ◽  
Magnetic Nanoparticle ◽  
Bottom Up ◽  
Novel Materials ◽  
Nanoparticle Clusters ◽  
Clustering Dynamics

Magnetically guided self-assembly of nanoparticles is a promising bottom-up method to fabricate novel materials and superstructures, such as, for example, magnetic nanoparticle clusters for biomedical applications. The existence of assembled...

scholarly journals Transient cell stiffening triggered by magnetic nanoparticle exposure

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Jose E. Perez ◽  
Florian Fage ◽  
David Pereira ◽  
Ali Abou-Hassan ◽  
Sophie Asnacios ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Magnetic Nanoparticles ◽  
Magnetic Nanoparticle ◽  
Early Time ◽  
Population Level ◽  
Short Time Scale ◽  
Initial Contact ◽  
Almost All ◽  
The Impact ◽  
Stiffening Effect

Abstract Background The interactions between nanoparticles and the biological environment have long been studied, with toxicological assays being the most common experimental route. In parallel, recent growing evidence has brought into light the important role that cell mechanics play in numerous cell biological processes. However, despite the prevalence of nanotechnology applications in biology, and in particular the increased use of magnetic nanoparticles for cell therapy and imaging, the impact of nanoparticles on the cells’ mechanical properties remains poorly understood. Results Here, we used a parallel plate rheometer to measure the impact of magnetic nanoparticles on the viscoelastic modulus G*(f) of individual cells. We show how the active uptake of nanoparticles translates into cell stiffening in a short time scale (< 30 min), at the single cell level. The cell stiffening effect is however less marked at the cell population level, when the cells are pre-labeled under a longer incubation time (2 h) with nanoparticles. 24 h later, the stiffening effect is no more present. Imaging of the nanoparticle uptake reveals almost immediate (within minutes) nanoparticle aggregation at the cell membrane, triggering early endocytosis, whereas nanoparticles are almost all confined in late or lysosomal endosomes after 2 h of uptake. Remarkably, this correlates well with the imaging of the actin cytoskeleton, with actin bundling being highly prevalent at early time points into the exposure to the nanoparticles, an effect that renormalizes after longer periods. Conclusions Overall, this work evidences that magnetic nanoparticle internalization, coupled to cytoskeleton remodeling, contributes to a change in the cell mechanical properties within minutes of their initial contact, leading to an increase in cell rigidity. This effect appears to be transient, reduced after hours and disappearing 24 h after the internalization has taken place.

Magnetic Nanoparticle Arrays with Ultra-Uniform Length Electrodeposited in Highly Ordered Alumina Nanopores(“Alumite”)

2000 ◽  
Vol 636 ◽  
Author(s):  
Robert M. Metzger ◽  
Ming Sun ◽  
Giovanni Zangari ◽  
Mohammad Shamsuzzoha
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Nanoparticle ◽  
Great Promise ◽  
Nanometer Scale ◽  
Nanoparticle Arrays ◽  
Low Aspect Ratio ◽  
Ordered Arrays ◽  
High Uniformity ◽  
Pulse Reverse ◽  
Hexagonally Ordered

AbstractWe report nanometer-scale ordered arrays of cylindrical magnetic nanoparticles with low aspect ratio and ultra-high uniformity. Protracted anodization provides hexagonally ordered nanopores in amorphous Al2O3. For instance, pulsed electrochemical deposition grows Co particles of uniform length from the bottoms of these pores: these particles are polycrystalline and randomly oriented. The magnetism of the array is dominated by particle shape and by inter-particle magnetostatic interactions. A very clear transition of the anisotropy from perpendicular to in-plane is observed at a height to radius ratio of about 2. This pulse-reverse electrodeposition shows great promise for a reliable synthesis of uniform nanostructures of many metals.

scholarly journals Magnetic nanoparticle-conjugated polymeric micelles for combined hyperthermia and chemotherapy

Nanoscale ◽  
2015 ◽  
Vol 7 (39) ◽  
pp. 16470-16480 ◽  
Author(s):  
Hyun-Chul Kim ◽  
Eunjoo Kim ◽  
Sang Won Jeong ◽  
Tae-Lin Ha ◽  
Sang-Im Park ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Synergistic Effect ◽  
Cancer Cells ◽  
Anticancer Drug ◽  
Magnetic Nanoparticle ◽  
Polymeric Micelles ◽  
Alternating Magnetic Field ◽  
Rapid Release

The cytotoxicity of magnetic nanoparticles-conjugated polymeric micelles encapsulated with an anticancer drug on cancer cells was enhanced by the synergistic effect of heat and the rapid release of the drug under an alternating magnetic field.

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