An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface Chemistries

Indium phosphide quantum dots (QDs) passivated with zinc sulphide in a core/shell architecture (InP/ZnS) with different surface chemistries were introduced to RAW 264.7 murine “macrophage-like” cells to understand their potential toxicities. The InP/ZnS quantum dots were conjugated with an oligonucleotide, a carboxylic acid, or an amino-polyethylene glycol ligand, and cell viability and cell proliferation were investigated via a metabolic assay. Membrane integrity was measured through the production of lactate dehydrogenase. Fluorescence microscopy showed cellular uptake. All quantum dots exhibited cytotoxic behaviour less than that observed from cadmium- or lead-based quantum dots; however, this behaviour was sensitive to the ligands used. In particular, the amino-polyethylene glycol conjugated quantum dots proved to possess the highest cytotoxicity examined here. This provides quantitative evidence that aqueous InP/ZnS quantum dots can offer a safer alternative for bioimaging or in therapeutic applications.

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In vitro investigation of individual and combined cytotoxic effects of aflatoxin B1 and other selected mycotoxins on the cell line porcine kidney 15

Experimental and Toxicologic Pathology ◽

10.1016/j.etp.2013.05.007 ◽

2013 ◽

Vol 65 (7-8) ◽

pp. 1149-1157 ◽

Cited By ~ 46

Author(s):

Mingyan Lei ◽

Niya Zhang ◽

Desheng Qi

Keyword(s):

Cell Line ◽

Aflatoxin B1 ◽

Porcine Kidney ◽

Cytotoxic Effects ◽

In Vitro Investigation

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In vitro investigation of individual and combined cytotoxic effects of ochratoxin A and other selected mycotoxins on renal cells

Toxicology in Vitro ◽

10.1016/j.tiv.2005.08.003 ◽

2006 ◽

Vol 20 (3) ◽

pp. 332-341 ◽

Cited By ~ 84

Author(s):

A.H. Heussner ◽

D.R. Dietrich ◽

E. O’Brien

Keyword(s):

Ochratoxin A ◽

Renal Cells ◽

Cytotoxic Effects ◽

In Vitro Investigation

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Characteristic synergistic cytotoxic effects toward cells in graphene oxide dressing with cadmium and copper ions

Toxicology Research ◽

10.1039/c9tx00146h ◽

2019 ◽

Vol 8 (6) ◽

pp. 908-917 ◽

Cited By ~ 2

Author(s):

Yiyang Dong ◽

Yulin Chang ◽

Haidi Gao ◽

Victoria Arantza León Anchustegui ◽

Qiang Yu ◽

...

Keyword(s):

Graphene Oxide ◽

Copper Ions ◽

Membrane Integrity ◽

In Vitro Toxicity ◽

Oxygen Species ◽

Human Beings ◽

Cell Toxicity ◽

Cytotoxic Effects

Abstract The increasing applications of graphene oxide (GO) in bio-medicine, environment and other fields enhance the exposure possibility of human beings to GO. Studies have been performed to address the in vitro toxicity of GO; however, little information on the in vivo biological consequence of GO with other common disasters is available, especially when cells are co-exposed to GO and common metal ions. To explore the influence and possible mechanisms of such co-exposure scenarios, a series of tests of cell viability, membrane integrity, reactive oxygen species (ROS), cell morphology, and Cd2+ distribution, were conducted. The results showed that the synergistic toxic mechanisms of GO and Cd2+, initiated from the adhesion of GO on HeLa cells, and followed by the recruitment of Cd2+ ions around the cell membrane, impaired the membrane integrity, morphology and adhesion capability, and triggered cell toxicity. The synergistic toxic mechanism of GO and Cu2+ mainly correlated to ROS, while no obvious relationship with membrane integrity was observed. The findings are envisaged to facilitate the application of GO in biology and related fields.

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In Vitro Investigation of the Cytotoxic Effects of Different Detergent-Containing Children's Toothpastes on Human Gingival Epithelial Cells

10.21203/rs.3.rs-885708/v1 ◽

2021 ◽

Author(s):

Sinem Birant ◽

Yazgul Duran ◽

Tunc Akkoc ◽

Figen Seymen

Keyword(s):

Epithelial Cells ◽

Third Molar ◽

Live Cell ◽

Control Group ◽

Dead Cell ◽

Cytotoxic Effects ◽

Gingival Epithelial Cells ◽

In Vitro Investigation ◽

Human Gingival Epithelial Cells

Abstract Background: This study aimed to evaluate possible cytotoxic effects to gingival epithelial cells exposed to children toothpastes containing different detergent. Methods: Tissues required fort he isolation of human gingival epithelial cells were obtained by biopsy during the extraction of the impacted third molar tooth. Toothpaste solutions of different concentrations were prepared from five different children’s toothpastes with different detergent contents. Isolated gingival epithelial cells were stimulated with experimental groups consisting of toothpaste solutions (Colgate, Sensodyne, Splat, Nenedent, Perlodent) at different concentrations and a control group consissting of complete Dulbocco’s modified eagle medium. After the experiments, cell viability was evaluated using flow cytometry. Data analysis were done using One Way ANOVA test and Tukey post-hoc test. Results: In all experimental groups, there was a decrease in live cell rates and an increase in dead cell rates due to increased concentration. The statistically highest live cell ratios were detected in Splat’s toothpaste solutions after the control group and the group with the lowest viability values was determined in Colgate group (p<0.05). Conclusions: According to the results of the study, it was observed that toothpastes containing SLS affected the viability of cells more negatively than toothpastes with other detergent contents.

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In VitroToxicity Evaluation of Engineered Cadmium-Coated Silica Nanoparticles on Human Pulmonary Cells

Journal of Toxicology ◽

10.1155/2013/931785 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 12

Author(s):

Uliana De Simone ◽

Luigi Manzo ◽

Antonella Profumo ◽

Teresa Coccini

Keyword(s):

Silica Nanoparticles ◽

Low Dose ◽

Membrane Integrity ◽

Apoptotic Pathway ◽

Cytotoxic Effects ◽

Pulmonary Cells ◽

Calcein Am ◽

Dose Dependent

Cytotoxicity of cadmium-containing silica nanoparticles Cd-SiO2NPs (0.05–100 µg/mL) versus SiO2NPs and CdCl2was evaluated by anin vitrotest battery in A549 by assessing (i) mitochondrial function, (ii) membrane integrity/cell morphology, (iii) cell growth/proliferation, (iv) apoptotic pathway, (v) oxidative stress, after short- (24–48 h) and long-term (10 days) exposure. Both Cd-SiO2NPs and CdCl2produced dose-dependent cytotoxic effects: (i)MTT-assay: similar cytotoxicity pattern was observed at both 24 and 48 h, with a more Cd-SiO2NPs pronounced effect than CdCl2. Cd-SiO2NPs induced mortality (about 50%) at 1 μg/mL, CdCl2at 25 μg/mL; (ii)calcein-AM/PI staining: decrease in cell viability, noticeable at 25 μg/mL, enhanced markedly at 50 and 100 μg/mL, after 24 h. Cd-SiO2NPs induced higher mortality than CdCl2(25% versus 4%, resp., at 25 μg/mL) with further exacerbation after 48h; (iii)clonogenic assay: exposure for longer period (10 days) compromised the A549 proliferative capacity at very low dose (0.05 μg/mL); (iv) a progressive activation ofcaspase-3 immunolabellingwas detected already at 1 μg/mL; (v) GSH intracellular level was modified by all compounds. In summary,in vitrodata demonstrated that both Cd-SiO2NPs and CdCl2affected all investigated endpoints, more markedly after Cd-SiO2NPs, while SiO2NPs influenced GSH only.

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Colloidal quantum dots suitability for long term cell imaging

10.1101/425850 ◽

2018 ◽

Author(s):

Patricia M. A. Farias ◽

André Galembeck ◽

Raquel Milani ◽

Wilson S. Mendonca ◽

Andreas Stingl

Keyword(s):

Quantum Dots ◽

Polyethylene Glycol ◽

Cell Viability ◽

Glial Cells ◽

Aqueous Suspension ◽

High Rate ◽

Term Monitoring ◽

Observation Period

AbstractFluorescent semiconductor nanoparticles in tree-dimensional quantum confinement, quantum dots (QDs), synthesized in aqueous medium, and functionalized with polyethylene glycol, were used as probes for the long-term imaging of glial cells. In vitro living healthy as well as cancer glial cells were labelled by direct insertion of a small volume of QDs contained in aqueous suspension into the culture wells. A long-term monitoring (over 7 days) of the cells was performed and no evidence of cell fixation and/or damage was observed. Two control groups, healthy and cancer glial cells, were used to compare cell viability. During the observation period, labelled and non labelled cells presented the same dynamics and no difference was observed regarding cell viability. To our knowledge, this is the first report of the viability of hydrophilic prepared quantum dots without any further surface treatment than the polyethylene-glycol coverage for the long-term imaging of living cells. Further, the study also permitted the observation of two distinct interaction mechanisms between cells and QDs. Healthy glial cells were mainly labelled at their surface, while non-healthy glial cells have shown a high rate in the uptake of QDs.

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Aptamer Conjugated Indium Phosphide Quantum Dots with a Zinc Sulphide Shell as Photoluminescent Labels for Acinetobacter baumannii

Nanomaterials ◽

10.3390/nano11123317 ◽

2021 ◽

Vol 11 (12) ◽

pp. 3317

Author(s):

Zeineb Ayed ◽

Shiana Malhotra ◽

Garima Dobhal ◽

Renee V. Goreham

Keyword(s):

Quantum Dots ◽

Indium Phosphide ◽

Acinetobacter Baumannii ◽

Photophysical Properties ◽

Zinc Sulphide ◽

Multi Drug Resistance ◽

Detection Mechanism ◽

Folded Structure ◽

Transmission Electron ◽

Recognition Function

Acinetobacter baumannii is a remarkable microorganism known for its diversity of habitat and its multi-drug resistance, resulting in hard-to-treat infections. Thus, a sensitive method for the identification and detection of Acinetobacter baumannii is vital. However, current methods used for the detection of pathogens have not improved in the past decades and suffer from long process times and low detection limits. A cheap, quick, and easy detection mechanism is needed. In this work, we successfully prepared indium phosphide quantum dots with a zinc sulphide shell, conjugated to a targeting aptamer ligand, to specifically label Acinetobacter baumannii. The system retained both the photophysical properties of the quantum dots and the folded structure and molecular recognition function of the aptamer, therefore successfully targeting Acinetobacter baumannii. Confocal microscopy and transmission electron microscopy showed the fluorescent quantum dots surrounding the Acinetobacter baumannii cells confirming the specificity of the aptamer conjugated to indium phosphide quantum dots with a zinc sulphide shell. Controls were undertaken with a different bacteria species, showing no binding of the aptamer conjugated quantum dots. Our strategy offers a novel method to detect bacteria and engineer a scalable platform for fluorescence detection, therefore improving current methods and allowing for better treatment.

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