scholarly journals Adiponectin: Structure, Physiological Functions, Role in Diseases, and Effects of Nutrition

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1180
Author(s):  
Kayvan Khoramipour ◽  
Karim Chamari ◽  
Amirhosein Ahmadi Hekmatikar ◽  
Amirhosein Ziyaiyan ◽  
Shima Taherkhani ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Amino Acids ◽  
Molecular Weight ◽  
Energy Regulation ◽  
Treatment Interventions ◽  
Adipose Tissues ◽  
Physiological Functions ◽  
Cellular Events ◽  
Healthy Nutrition

Adiponectin (a protein consisting of 244 amino acids and characterized by a molecular weight of 28 kDa) is a cytokine that is secreted from adipose tissues (adipokine). Available evidence suggests that adiponectin is involved in a variety of physiological functions, molecular and cellular events, including lipid metabolism, energy regulation, immune response and inflammation, and insulin sensitivity. It has a protective effect on neurons and neural stem cells. Adiponectin levels have been reported to be negatively correlated with cancer, cardiovascular disease, and diabetes, and shown to be affected (i.e., significantly increased) by proper healthy nutrition. The present review comprehensively overviews the role of adiponectin in a range of diseases, showing that it can be used as a biomarker for diagnosing these disorders as well as a target for monitoring the effectiveness of preventive and treatment interventions.

scholarly journals Reconstitution in Proteoliposomes of the Recombinant Human Riboflavin Transporter 2 (SLC52A2) Overexpressed in E. coli

2019 ◽  
Vol 20 (18) ◽  
pp. 4416 ◽  
Author(s):  
Lara Console ◽  
Maria Tolomeo ◽  
Matilde Colella ◽  
Maria Barile ◽  
Cesare Indiveri
Keyword(s):  
Cardiovascular Disease ◽  
Amino Acids ◽  
Risk Factor ◽  
Biologically Active ◽  
Suitable Model ◽  
Native Protein ◽  
E Coli ◽  
Few Data ◽  
Riboflavin Transport

Background: the SLC52A2 gene encodes for the riboflavin transporter 2 (RFVT2). This transporter is ubiquitously expressed. It mediates the transport of Riboflavin across cell membranes. Riboflavin plays a crucial role in cells since its biologically active forms, FMN and FAD, are essential for the metabolism of carbohydrates, amino acids, and lipids. Mutation of the Riboflavin transporters is a risk factor for anemia, cancer, cardiovascular disease, neurodegeneration. Inborn mutations of SLC52A2 are associated with Brown-Vialetto-van Laere syndrome, a rare neurological disorder characterized by infancy onset. In spite of the important metabolic and physio/pathological role of this transporter few data are available on its function and regulation. Methods: the human recombinant RFVT2 has been overexpressed in E. coli, purified and reconstituted into proteoliposomes in order to characterize its activity following the [3H]Riboflavin transport. Results: the recombinant hRFVT2 showed a Km of 0.26 ± 0.07 µM and was inhibited by lumiflavin, FMN and Mg2+. The Riboflavin uptake was also regulated by Ca2+. The native protein extracted from fibroblast and reconstituted in proteoliposomes also showed inhibition by FMN and lumiflavin. Conclusions: proteoliposomes represent a suitable model to assay the RFVT2 function. It will be useful for screening the mutation of RFVT2.

scholarly journals Plasma Branched-Chain Amino Acids and Incident Cardiovascular Disease in the PREDIMED Trial

2016 ◽  
Vol 62 (4) ◽  
pp. 582-592 ◽  
Author(s):  
Miguel Ruiz-Canela ◽  
Estefania Toledo ◽  
Clary B Clish ◽  
Adela Hruby ◽  
Liming Liang ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Amino Acids ◽  
Cardiovascular Death ◽  
Branched Chain Amino Acids ◽  
Control Group ◽  
Cvd Risk ◽  
Hazard Ratios ◽  
Increased Risk ◽  
Branched Chain

Abstract BACKGROUND The role of branched-chain amino acids (BCAAs) in cardiovascular disease (CVD) remains poorly understood. We hypothesized that baseline BCAA concentrations predict future risk of CVD and that a Mediterranean diet (MedDiet) intervention may counteract this effect. METHODS We developed a case-cohort study within the Prevención con Dieta Mediterránea (PREDIMED), with 226 incident CVD cases and 744 noncases. We used LC-MS/MS to measure plasma BCAAs (leucine, isoleucine, and valine), both at baseline and after 1 year of follow-up. The primary outcome was a composite of incident stroke, myocardial infarction, or cardiovascular death. RESULTS After adjustment for potential confounders, baseline leucine and isoleucine concentrations were associated with higher CVD risk: the hazard ratios (HRs) for the highest vs lowest quartile were 1.70 (95% CI, 1.05–2.76) and 2.09 (1.27–3.44), respectively. Stronger associations were found for stroke. For both CVD and stroke, we found higher HRs across successive quartiles of BCAAs in the control group than in the MedDiet groups. With stroke as the outcome, a significant interaction (P = 0.009) between baseline BCAA score and intervention with MedDiet was observed. No significant effect of the intervention on 1-year changes in BCAAs or any association between 1-year changes in BCAAs and CVD were observed. CONCLUSIONS Higher concentrations of baseline BCAAs were associated with increased risk of CVD, especially stroke, in a high cardiovascular risk population. A Mediterranean-style diet had a negligible effect on 1-year changes in BCAAs, but it may counteract the harmful effects of BCAAs on stroke.

scholarly journals Integrated Role of Nutrition and Physical Activity for Lifelong Health

Nutrients ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1437 ◽  
Author(s):  
Karsten Koehler ◽  
Clemens Drenowatz
Keyword(s):  
Physical Activity ◽  
Diabetes Mellitus ◽  
Cardiovascular Disease ◽  
Body Composition ◽  
Metabolic Diseases ◽  
Musculoskeletal Health ◽  
Healthy Nutrition ◽  
Improve Body Composition ◽  
Nutrition And Physical Activity

It is well established that healthy nutrition and physical activity (PA) are key lifestyle factors that modulate lifelong health through their ability to improve body composition, musculoskeletal health, and physical and cognitive performance, as well as to prevent metabolic diseases including obesity, diabetes mellitus, and cardiovascular disease across the lifespan [...]

Excretion by the Malpighian Tubules of the Stick Insect, Dixippus Morosus (Orthoptera, Phasmidae): Amino Acids, Sugars and Urea

1958 ◽  
Vol 35 (4) ◽  
pp. 871-891 ◽  
Author(s):  
J. A. RAMSAY
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Stick Insect ◽  
Passive Diffusion ◽  
Malpighian Tubules ◽  
Excretory System ◽  
Low Molecular Weight ◽  
Kinetics Of

1. Contrary to expectation, it is found that metabolically useful substances pass into the urine and are reabsorbed in the rectum. 2. The kinetics of penetration have been investigated for six amino acids, three sugars and urea. 3. The evidence indicates that these substances enter the urine by passive diffusion. 4. The role of the Malpighian tubules in the insect's excretory system is discussed in the light of these findings. It is suggested that the tubule is primarily a means whereby all soluble substances of low molecular weight are removed from the haemolymph and that in this respect it has analogies with the glomerulus as well as with the tubule of the vertebrate nephron.

Effect of homo poly(L-amino acids) on fibrin assembly: role of charge and molecular weight

Biochemistry ◽  
1989 ◽  
Vol 28 (3) ◽  
pp. 1384-1388 ◽  
Author(s):  
Marcus E. Carr ◽  
Roy Cromartie ◽  
Don A. Gabriel
Keyword(s):  
Amino Acids ◽  
Molecular Weight

scholarly journals Properties of inorganic pyrophosphatase of pig scapula cartilage

1975 ◽  
Vol 147 (1) ◽  
pp. 111-118 ◽  
Author(s):  
R Felix ◽  
H Fleisch
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Alkaline Phosphatase ◽  
Metal Ions ◽  
Ammonium Molybdate ◽  
Inorganic Pyrophosphatase ◽  
Ph Optimum ◽  
Km Value ◽  
Calcification Process

The properties of a highly purified inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC 3.6.1.1) from pig scapula cartilage were studied. The enzyme had a molecular weight of 66 000 and a pH optimum of 7-8. It was markedly activated by magnesium, but not, or only to a much smaller degree, by other metal ions. PP1 was the only substrate found and had a Km value of 11 muM. The enzyme was not inhibited by phosphate and other inhibitors of alkaline phosphatase such as CN- minus, amino acids and theophylline; it was slightly inhibited by tartrate, formaldehyde and ammonium molybdate and strongly inhibited by F- minus, Ca2+ and other metal ions. The properties of the enzyme in the presence of concentrations of PP1 present in plasma (3.5 muM) were similar to those found at higher (2 mM) concentrations of PP1. The diphosphonates ethane-1-hydroxy-1,1-diphosphonate and dichloromethylenediphosphonate inhibited the enzyme in the presence of low PP1 concentrations. The characteristics of this enzyme are therefore similar to pyrophosphatases from other sources, such as from yeast and erythrocytes, and do not support a specific role of this enzyme in the calcification process.

scholarly journals Dietary Composition and Cardiovascular Risk: A Mediator or a Bystander?

Nutrients ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1912 ◽  
Author(s):  
Emmanouil Korakas ◽  
George Dimitriadis ◽  
Athanasios Raptis ◽  
Vaia Lambadiari
Keyword(s):  
Cardiovascular Disease ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Treatment Interventions ◽  
Metabolic Derangement ◽  
Metabolic Markers ◽  
Low Carbohydrate ◽  
New Treatment ◽  
Fat Diets

The role of nutrition in the pathogenesis of cardiovascular disease has long been debated. The established notion of the deleterious effects of fat is recently under question, with numerous studies demonstrating the benefits of low-carbohydrate, high-fat diets in terms of obesity, diabetes, dyslipidemia, and metabolic derangement. Monounsaturated and polyunsaturated fatty acids, especially n-3 PUFAs (polyunsaturated fatty acids), are the types of fat that favor metabolic markers and are key components of the Mediterranean Diet, which is considered an ideal dietary pattern with great cardioprotective effects. Except for macronutrients, however, micronutrients like polyphenols, carotenoids, and vitamins act on molecular pathways that affect oxidative stress, endothelial function, and lipid and glucose homeostasis. In relation to these metabolic markers, the human gut microbiome is constantly revealed, with its composition being altered by even small dietary changes and different microbial populations being associated with adverse cardiovascular outcomes, thus becoming the target for potential new treatment interventions. This review aims to present the most recent data concerning different dietary patterns at both the macro- and micronutrient level and their association with atherosclerosis, obesity, and other risk factors for cardiovascular disease.

scholarly journals What is the Actual Molecular Weight of Irisin Hormone According to Western Blot Analysis?

2019 ◽  
pp. 40-41
Author(s):  
Kader Ugur ◽  
Suleyman Aydin
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Molecular Weight ◽  
Tandem Mass Spectrometry ◽  
Western Blot ◽  
Blot Analysis ◽  
Tandem Mass ◽  
Adipose Tissues ◽  
Cardiac Muscles ◽  
Dimeric Protein

Irisin hormone, secreted mainly in skeletal, cardiac muscles, is proteolytically cleaved from the C-terminal moiety and secreted from the fibronectin domain-containing protein 5(FNDC5) receptor. This hormone carries carbohydrate moieties, which are glycosylated, and is a dimeric protein, and released as a hormone of 112 amino acids [1]. The dimerization of this hormone is not affected by glycosylation, although N-glycosylation is necessary for the stabilization of FNDC5 and secretion of irisin [2]. Quantitation of circulating human irisin by Tandem Mass Spectrometry was ∼ 3.6 ng/ml in sedentary individuals [3]. Irisin is secreted mainly in skeletal, cardiac muscles and adipose tissues.

E. coli Maltodextrin Phosphorylase: Primary Structure and Deletion Mapping of the C-Terminal Site

1987 ◽  
Vol 42 (4) ◽  
pp. 394-400 ◽  
Author(s):  
D. Palm ◽  
R. Goerl ◽  
G. Weidinger ◽  
R. Zeier ◽  
B. Fischer
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Pyridoxal Phosphate ◽  
Deletion Mapping ◽  
E Coli ◽  
Calculated Molecular Weight ◽  
C Terminus ◽  
Maltodextrin Phosphorylase ◽  
Terminal Site

Abstract The complete 796 residue amino acid sequence of maltodextrin phosphorylase was deduced from the E. coli malP nucleotide sequence. The calculated molecular weight of 90 500, including pyridoxal phosphate, is significantly larger than experimentally determined values. Enzymatically active and inactive mutants following deletion or exchange of up to 8 codons (7 amino acids) at the 3′ end (C-terminus) confirm the size of the mature native enzyme and disclose the essential functional or structural role of the highly conserved C-terminal region of phosphorylases.

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