Comparative Genomics of Clinical and Environmental Isolates of Vibrio spp. of Colombia: Implications of Traits Associated with Virulence and Resistance

There is widespread concern about the increase in cases of human and animal infections caused by pathogenic Vibrio species due to the emergence of epidemic lineages. In Colombia, active surveillance by the National Institute of Health (INS) has confirmed the presence of Vibrio; however, in routine surveillance, these isolates are not genomically characterized. This study focused on the pangenome analysis of six Vibrio species: V. parahaemolyticus, V. vulnificus, V. alginolyticus, V. fluvialis, V. diabolicus and V. furnissii to determine the genetic architectures of potentially virulent and antimicrobial resistance traits. Isolates from environmental and clinical samples were genome sequenced, assembled and annotated. The most important species in public health were further characterized by multilocus sequence typing and phylogenomics. For V. parahaemolyticus, we found the virulent ST3 and ST120 genotypes. For V. vulnificus, we identified isolates belonging to lineages 1 and 2. Virulence gene homologues between species were found even in non-pathogenic species such as V. diabolicus. Annotations related to the mobilome, integrative mobile and conjugative elements and resistance genes were obtained from environmental and clinical isolates. This study contributes genomic information to the intensified surveillance program implemented by the INS to establish potential sources of vibriosis in Colombia.

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The Impact of Water Intrusion on Pathogenic Vibrio Species to Inland Brackish Waters of China

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17186781 ◽

2020 ◽

Vol 17 (18) ◽

pp. 6781

Author(s):

Qingyao Wang ◽

Songzhe Fu ◽

Qian Yang ◽

Jingwei Hao ◽

Can Zhou ◽

...

Keyword(s):

Seawater Intrusion ◽

Water Area ◽

Vibrio Species ◽

Most Probable Number ◽

Potential Health ◽

Probable Number ◽

Inland River ◽

Pathogenic Vibrio ◽

The Impact ◽

Vibrio Spp

The estuary is the ecological niche of pathogenic Vibrio spp. as it provides abundant organic and inorganic nutrients from seawater and rivers. However, little is known about the ecology of these Vibrio species in the inland brackish water area. In this study, their co-occurrence and relationships to key environmental constraints (salinity and temperature) in the Hun-Tai River of China were examined using the most probable number polymerase chain reaction (MPN-PCR) approach. We hereby report 2-year continuous surveillance based on six water indices of the Hun-Tai River. The results showed that seawater intrusion maximally reached inland as far as 26.5 km for the Hun-Tai River. Pathogenic Vibrio spp. were detected in 21.9% of the water samples. In particular, V. cholerae, V. parahaemolyticus, and V. vulnificus were isolated in 10 (10.4%), 20 (20.8.5%), and 2 (2.08%) samples, respectively. All V. parahaemolyticus strains were tdh gene negative, 10% were positive for the trh gene. Multi-locus sequence typing (MLST) divided V. parahaemolyticus strains into 12 sequence types (STs) for the Hun-Tai River. Five STs were respectively present in various locations along the Hun-Tai River. The PCR assay for detecting six virulence genes and Vibrio seventh pandemic island I and II revealed three genotypes in 12 V. cholerae isolates. The results of our study showed that seawater intrusion and salinity have profound effects on the distribution of pathogenic Vibrio spp. in the inland river, suggesting a potential health risk associated with the waters of the Hun-Tai River used for irrigation and drinking.

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Temporal and Spatial Variability in Culturable Pathogenic Vibrio spp. in Lake Pontchartrain, Louisiana, following Hurricanes Katrina and Rita

Applied and Environmental Microbiology ◽

10.1128/aem.02509-10 ◽

2011 ◽

Vol 77 (15) ◽

pp. 5384-5393 ◽

Cited By ~ 24

Author(s):

Olivia D. Nigro ◽

Aixin Hou ◽

Gayatri Vithanage ◽

Roger S. Fujioka ◽

Grieg F. Steward

Keyword(s):

Virulence Gene ◽

Lake Pontchartrain ◽

Data Set ◽

Content Type ◽

Pathogenic Vibrio ◽

Temporal And Spatial Variability ◽

Hurricanes Katrina And Rita ◽

Pathogenic Vibrios ◽

Vibrio Spp ◽

Sampling Times

ABSTRACTWe investigated the abundance, distribution, and virulence gene content ofVibrio cholerae,V. parahaemolyticus, andV. vulnificusin the waters of southern Lake Pontchartrain in Louisiana on four occasions from October 2005 to September 2006, using selective cultivation and molecular assays. The three targeted pathogenic vibrios were generally below the detection level in January 2006, when the water was cold (13°C), and most abundant in September 2006, when the lake water was warmest (30°C). The maximum values for these species were higher than reported previously for the lake by severalfold to orders of magnitude. The only variable consistently correlated with total vibrio abundance within a single sampling was distance from shore (P= 0.000). Multiple linear regression of the entire data set revealed that distance from shore, temperature, and turbidity together explained 82.1% of the variability in total vibrio CFU. The log-transformed mean abundance ofV. vulnificusCFU in the lake was significantly correlated with temperature (P= 0.014), but not salinity (P= 0.625). Virulence-associated genes ofV. cholerae(ctx) andV. parahaemolyticus(trhandtdh) were not detected in any isolates of these species (n= 128 andn= 20, respectively). In contrast, 16S rRNA typing ofV. vulnificus(n= 298) revealed the presence of both environmental (type A) and clinical (type B) strains. The percentage of the B-typeV. vulnificuswas significantly higher in the lake in October 2005 (35.8% of the total) than at other sampling times (P≤ 0.004), consistent with the view that these strains represent distinct ecotypes.

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Sensitivity of Vibrio parahaemolyticus, V. vulnificus and V. harveyi Against Chloroxylenol (4-Chloro-3,5-dimethylphenol , C8H9ClO) Antiseptic and Pine Oil Disinfectant

Jurnal Kelautan Tropis ◽

10.14710/jkt.v23i3.9126 ◽

2020 ◽

Vol 23 (3) ◽

pp. 381-387

Author(s):

Subagiyo Subagiyo ◽

Ervia Yudiati ◽

Nuril Azhar ◽

Rabia Alghazeer

Keyword(s):

Active Compound ◽

Vibrio Parahaemolyticus ◽

Inhibition Zone ◽

Vibrio Species ◽

Disk Diffusion ◽

Shrimp Pond ◽

Pine Oil ◽

Pathogenic Vibrio ◽

Agar Media ◽

Vibrio Spp

Vibrio spp. genus is known as a marine indigeneous bacteria. Vibrio parahaemolyticus, V. vulnificus and V. harveyi are pathogenic Vibrio. This study aims to assess the sensitivity of three Vibrio species (V parahaemolyticus, V. vulnificus and V. harveyi) isolated from shrimp pond against two type of disinfectant with different active compound namely Chloroxylenol (4-Chloro-3,5-dimethylphenol, C8H9ClO) and pine oil. The assessment was done by Kirby-Bauer disk diffusion methods in Zobell agar media with two different concentration (10 and 100 ppm) and replicated in three times. Sensitivity of Vibrio spp. was analized based on the inhibition zone activity produced by disinfectant. Results showed that sensitivity of Vibrio spp. against disinfectant Chloroxylenol 4.8% at 100 ppm were higher than 10 ppm. The increment of V parahaemolyticus was 182 %, V. vulnificus was 47 % and V. harveyi was 43 %, respectively. Susceptibility of antiseptic with Chloroxylenol 4.8% at 100 ppm was arised to 152 % (V. parahaemolyticus), 43 % (V. vulnificus) and 31 % (V. harveyi) when compared to 2.5% pine oil disinfectant. It can be concluded that Chloroxylenol 4,8 % active compound and pine oil were able to inhibit the Vibrio spp. growth.

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Vibrio Species in an Urban Tropical Estuary: Antimicrobial Susceptibility, Interaction with Environmental Parameters, and Possible Public Health Outcomes

Microorganisms ◽

10.3390/microorganisms9051007 ◽

2021 ◽

Vol 9 (5) ◽

pp. 1007

Author(s):

Anna L. B. Canellas ◽

Isabelle R. Lopes ◽

Marianne P. Mello ◽

Rodolfo Paranhos ◽

Bruno F. R. de Oliveira ◽

...

Keyword(s):

Antimicrobial Susceptibility ◽

Environmental Parameters ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

Vibrio Species ◽

Beta Lactam ◽

Pathogenic Vibrio ◽

Extended Spectrum Beta Lactamases ◽

Nitrogen Concentrations ◽

Vibrio Spp

The genus Vibrio comprises pathogens ubiquitous to marine environments. This study evaluated the cultivable Vibrio community in the Guanabara Bay (GB), a recreational, yet heavily polluted estuary in Rio de Janeiro, Brazil. Over one year, 66 water samples from three locations along a pollution gradient were investigated. Isolates were identified by MALDI-TOF mass spectrometry, revealing 20 Vibrio species, including several potential pathogens. Antimicrobial susceptibility testing confirmed resistance to aminoglycosides, beta-lactams (including carbapenems and third-generation cephalosporins), fluoroquinolones, sulfonamides, and tetracyclines. Four strains were producers of extended-spectrum beta-lactamases (ESBL), all of which carried beta-lactam and heavy metal resistance genes. The toxR gene was detected in all V. parahaemolyticus strains, although none carried the tdh or trh genes. Higher bacterial isolation rates occurred in months marked by higher water temperatures, lower salinities, and lower phosphorus and nitrogen concentrations. The presence of non-susceptible Vibrio spp. was related to indicators of eutrophication and sewage inflow. DNA fingerprinting analyses revealed that V. harveyi and V. parahaemolyticus strains non-susceptible to antimicrobials might persist in these waters throughout the year. Our findings indicate the presence of antimicrobial-resistant and potentially pathogenic Vibrio spp. in a recreational environment, raising concerns about the possible risks of human exposure to these waters.

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Prevalence, antimicrobial resistance profiles and virulence genes of Vibrio spp. isolated from shrimp retails in Ho Chi Minh City (Vietnam)

The Journal of Agriculture and Development ◽

10.52997/jad.5.03.2019 ◽

2019 ◽

Vol 18 (03) ◽

pp. 27-34

Author(s):

Trinh N. T. Huynh

Keyword(s):

Antimicrobial Resistance ◽

Virulence Genes ◽

Antimicrobial Agents ◽

Common Species ◽

Vibrio Species ◽

Ho Chi Minh City ◽

Pathogenic Vibrio ◽

Food Borne ◽

Food Quality And Safety ◽

Vibrio Spp

This study was conducted to determine the diversity of pathogenic Vibrio species, the antimicrobial resistance profile and the presence of virulence genes linked to food-borne pathogens of Vibrio spp. isolated from shrimp samples in Ho Chi Minh City, Vietnam. A total of 40 raw shrimp batches were collected from retails markets (supermarket and street). All 133 test strains were isolated from 40 shrimp samples. V. parahaemolyticus was the most common species (87.5%), followed by V. navarrensis (60%), V. alginolyticus (52.5%), V. cholerae non-O1 (37.5%), V. vulnificus (22.5%), and V. fluvialis (10%). Vibrio spp. isolates were susceptible to 12 antimicrobial agents. The prevalence of ampicillin resistance was highest (82.7%), followed by cotrimoxazole (18.8%) and 3rd generation cephalosporins (16.5% cefotaxime and 8.3% ceftazidime). Extended spectrum β lactamase (ESBL) activity was detected in 28.1% V. parahaemolyticus isolates. None of tdh or trh virulence genes were detected. The results of this study indicated the presentation of Vibrio species in shrimp samples purchased in Ho Chi Minh City. Therefore, our results could be of great potential for the identification of Vibrio infection in shrimp samples taken from different regions to improve food quality and safety.

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Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp.

Clinical Microbiology Reviews ◽

10.1128/cmr.1.3.245 ◽

1988 ◽

Vol 1 (3) ◽

pp. 245-267 ◽

Cited By ~ 237

Author(s):

J M Janda ◽

C Powers ◽

R G Bryant ◽

S L Abbott

Keyword(s):

Clinical History ◽

Vibrio Species ◽

Human Pathogens ◽

Isolation And Identification ◽

Virulence Potential ◽

Pathogenic Vibrio ◽

Common Clinical Presentation ◽

Vibrio Cholerae O1 ◽

Clinically Significant ◽

Vibrio Spp

Recent taxonomic advances have now implicated several different Vibrio species as human pathogens. While the most common clinical presentation of Vibrio infection continues to be gastroenteritis, an increasing number of extraintestinal infections are being reported, particularly in immunocompromised individuals. Detection of Vibrio infections requires a good clinical history and the use of appropriate isolation and identification procedures by the laboratory to confirm illnesses attributed to Vibrio species. Except for Vibrio cholerae O1 and Vibrio parahaemolyticus, there is little direct evidence linking the production of a myriad of cell-associated or extracellular factors produced by each species with human disease and pathogenesis. Many questions regarding pathogenic Vibrio species remain unanswered, including their frequency and distribution in environmental specimens (water, shellfish), infective doses, virulence potential of individual isolates, and markers associated with such strains.

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Coastal Microbiomes Reveal Associations between Pathogenic Vibrio Species, Environmental Factors, and Planktonic Communities

10.21203/rs.3.rs-100087/v1 ◽

2020 ◽

Author(s):

Rachel E. Diner ◽

Drishti Kaul ◽

Ariel Rabines ◽

Hong Zheng ◽

Joshua A Steele ◽

...

Keyword(s):

Antibiotic Resistance ◽

Microbial Communities ◽

Antibiotic Resistance Genes ◽

Human Infection ◽

Vibrio Species ◽

Model Systems ◽

Laboratory Model ◽

Environmental Preferences ◽

Pathogenic Vibrio ◽

Vibrio Spp

Abstract Background Many species of coastal Vibrio spp. bacteria can infect humans, representing an emerging health threat linked to increasing seawater temperatures. Vibrio interactions with the planktonic community impact coastal ecology and human infection potential. In particular, interactions with eukaryotic and photosynthetic organism may provide attachment substrate and critical nutrients (e.g. chitin, phytoplankton exudates) that facilitate the persistence, diversification, and spread of pathogenic Vibrio spp.. Vibrio interactions with these organisms in an environmental context are, however, poorly understood. Results After quantifying pathogenic Vibrio species, including V. cholerae , V. parahaemolyticus , and V. vulnificus, over one year at 5 sites, we found that all three species reached high abundances, particularly during Summer months, and exhibited species-specific temperature and salinity distributions. Using metabarcoding we established a detailed profile of the both prokaryotic and eukaryotic coastal microbial communities, finding that pathogenic species were frequently associated with specific ASVs of chitin-producing eukaryotes such as diatoms and copepods. Furthermore, environmental variables had a significant effect not only on pathogenic Vibrio species but entire microbial communities, suggesting in some cases shared environmental preferences. Several significant ASV-level associations were revealed, indicating that commonly used broad taxonomic classifications (e.g. based on microbial class or Vibrio as a genus) likely mask ecologically important interactions. Shotgun metagenomic analyses revealed diverse vibrio communities that harbored additional potential vibrio pathogens, antibiotic resistance genes, and genes associated with virulence. Conclusions Taken together, this data shows that abundant pathogenic Vibrio species likely containing both antibiotic resistance and virulence-associated genes are associated with chitin producing organisms which could act as an attachment substrate, facilitating environmental persistence and horizontal gene transfer. Shared environmental conditions such as high temperatures were associated with both high levels of pathogenic vibrios and potential environmental reservoirs, which should be taken into consideration when modelling vibrio infection risk in the face of climate change and identifying biomarkers of pathogen species. Furthermore, ASV-level associations may be critical to understanding vibrio microbial ecology and should be taken into consideration while developing environmentally relevant laboratory model systems.

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Benthic ecology of Vibrio spp. and pathogenic Vibrio species in a coastal Mediterranean environment (La Spezia Gulf, Italy)

Microbial Ecology ◽

10.1007/s00248-009-9542-8 ◽

2009 ◽

Vol 58 (4) ◽

pp. 808-818 ◽

Cited By ~ 61

Author(s):

Luigi Vezzulli ◽

◽

Elisabetta Pezzati ◽

Mariapaola Moreno ◽

Mauro Fabiano ◽

...

Keyword(s):

Vibrio Species ◽

Benthic Ecology ◽

Mediterranean Environment ◽

Pathogenic Vibrio ◽

Vibrio Spp

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Environmental Isolates of Azole-Resistant Aspergillus fumigatus in Germany

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00100-15 ◽

2015 ◽

Vol 59 (7) ◽

pp. 4356-4359 ◽

Cited By ~ 59

Author(s):

Oliver Bader ◽

Jana Tünnermann ◽

Anna Dudakova ◽

Marut Tangwattanachuleeporn ◽

Michael Weig ◽

...

Keyword(s):

Drug Resistance ◽

Aspergillus Fumigatus ◽

Antifungal Drug ◽

Clinical Samples ◽

Environmental Isolates ◽

Northern Germany ◽

Content Type ◽

Antifungal Drug Resistance ◽

The World

ABSTRACTAzole antifungal drug resistance inAspergillus fumigatusis an emerging problem in several parts of the world. Here we investigated the distribution of such strains in soils from Germany. At a general positivity rate of 12%, most prevalently, we found strains with the TR34/L98H and TR46/Y121F/T289A alleles, dispersed along a corridor across northern Germany. Comparison of the distributions of resistance alleles and genotypes between environment and clinical samples suggests the presence of local clinical clusters.

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Assessment and Antibiotic Resistance Profiling in Vibrio Species Isolated from Wild Birds Captured in Danube Delta Biosphere Reserve, Romania

Antibiotics ◽

10.3390/antibiotics10030333 ◽

2021 ◽

Vol 10 (3) ◽

pp. 333

Author(s):

Emöke Páll ◽

Mihaela Niculae ◽

Gheorghe F. Brudașcă ◽

Rustam Kh. Ravilov ◽

Carmen Dana Șandru ◽

...

Keyword(s):

Antibiotic Resistance ◽

Multidrug Resistance ◽

Migratory Birds ◽

Habitat Type ◽

Wild Birds ◽

Vibrio Species ◽

Resistant Bacteria ◽

Danube Delta ◽

Increased Risk ◽

Vibrio Spp

Antimicrobial and multidrug-resistant bacteria are a major problem worldwide and, consequently, the surveillance of antibiotic-resistant bacteria and assessment of the dissemination routes are essential. We hypothesized that migratory birds, coming from various environments, would carry more numerous Vibrio strains than sedentary species, with increased risk to be passed to their contacts or environment in habitats they transit or nest in. Similarly, we presumed that strains from migratory birds will show multidrug resistance. A total of 170 oral and rectal swabs were collected from wild birds captured in different locations of the Danube Delta (Malic, Sfantu-Gheorghe, Letea Forest) and processed using standardized selective media. V. cholerae strains were confirmed by serology and molecular methods and, subsequently, their susceptibility was evaluated. The prevalence of Vibrio species by host species, habitat type, and location was interpreted. The isolated Vibrio species were identified as Vibrio cholerae 14.33%, V. fluvialis 13.33%, V. alginolyticus 12%, V. mimicus 17.33%, V. vulnificus 10.88%, with V. parahaemolyticus and V. metschnikovii (16%) also being prevalent. Of the 76 Vibrio spp. isolates, 18.42% were resistant towards at least three antimicrobials, and 81.57% demonstrated a multidrug resistance phenotype, including mainly penicillins, aminoglycosides, and macrolides. The results of the present study indicate higher numbers of Vibrio strains in migratory (74.66%) than in sedentary birds (25.33%), confirming our hypothesis. Furthermore, the increased pathogenicity of Vibrio spp. strains, isolated from wild migratory and sedentary birds, was confirmed by their increased multiple antibiotic resistance (MAR) index (0.09–0.81).

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