Oxypeucedanin: Chemotaxonomy, Isolation, and Bioactivities

The present review comprehensively gathered phytochemical, bioactivity, and pharmacokinetic reports on a linear furanocoumarin, namely oxypeucedanin. Oxypeucedanin (OP), which structurally contains an epoxide ring, has been majorly isolated from ethyl acetate-soluble partitions of several genera, particularly Angelica, Ferulago, and Prangos of the Apiaceae family; and Citrus, belonging to the Rutaceae family. The methanolic extract of Angelica dahurica roots has been analytically characterized as the richest natural OP source. This naturally occurring secondary metabolite has been described to possess potent antiproliferative, cytotoxic, anti-influenza, and antiallergic activities, as assessed in preclinical studies. In order to explore potential drug candidates, oxypeucedanin, its derivatives, and semi-synthetically optimized analogues can be considered for the complementary assessments of biological assays.

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Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest

Indonesian Journal of Pharmaceutical Science and Technology ◽

10.24198/ijpst.v5i2.15364 ◽

2018 ◽

Vol 5 (2) ◽

pp. 61

Author(s):

Yohannes Alen ◽

Atika Melati ◽

Gemmy Sarina ◽

Akmal Djamaan

Keyword(s):

Melting Point ◽

Ethyl Acetate ◽

Secondary Metabolite ◽

Methanol Extract ◽

Methanolic Extract ◽

Ethyl Acetate Fraction ◽

Sample Weight ◽

Chromatography Method ◽

White Needle ◽

Layer Chromatography

Aspergillus niger is pathogen fungi that can live in various locations and can live contiguous with many hosts, one of them is queen termite’s nest. The aims of the study were to isolated the secondary metabilite of A.niger. Extraction proccess of secondary metabolite compounds was carried out by maceration method using methanol solvent. Based on that proccess, methanol extract was be yield 4,32% sample weight. Fractination proccess was carried out in the separating funnel using ethyl acetate solvent, which ethyl acetate fraction was be yield 14.39% methanol extract. Separation of the compounds was carried out by column chromatography method using n-hexane and ethyl asetate eluents. Purification of the compounds were done by recrystallization method using n-hexane and ethyl asetate. Two secondary metabolite compounds were successfully isolated from ethyl acetate fraction of the methanolic extract of fungus A. niger “In-Habiting” queen termite’s nest M. gilvus Hagen. Based on organoleptic examination, the compound signed AM-12-22-01 is 35 mg, white needle crystals, melting point 151-153 oC. While, the AM-12-60-01* is 15 mg, white needle crystals, melting point 91-93 oC. Based on the chemical analysis, thin layer chromatography, ultraviolet and infrared spectra data it was identified that AM-12-22-01 and AM-12-60-01 were a phenolic compounds.Key words: isolation, A. niger, In-Habiting, M. gilvus Hagen.

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Antioxidant and Anti-inflammatory Activities of Organic and Aqueous Extracts of Northeast Algerian Marrubium vulgare

Phytothérapie ◽

10.3166/phyto-2018-0106 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S119-S129

Author(s):

I. Namoune ◽

B. Khettal ◽

A.M. Assaf ◽

S. Elhayek ◽

L. Arrar

Keyword(s):

Ethyl Acetate ◽

Methanolic Extract ◽

Ethyl Acetate Extract ◽

Interleukin 1 ◽

Total Phenolic ◽

Dependent Manner ◽

Aqueous Extracts ◽

Traditional Use ◽

Marrubium Vulgare ◽

Anti Inflammatory

Marrubium vulgare (Lamiaceae) is frequently used in traditional medicine to treat many illnesses from ancient times. Its beneficial effects include antibacterial, antioedematogenic, and analgesic activities. This study was designed to evaluate the antioxidant and anti-inflammatory activities of organic and aqueous extracts of the leaves, the flowers, the stems, and the roots of Marrubium vulgare. The total phenolic and flavonoid contents as well as the antioxidant and the anti-inflammatory effects of methanol, chloroform, ethyl acetate, and aqueous extracts have been investigated by using different in-vitro methods. It was found that the ethyl acetate extract from Marrubium vulgare stems had the highest total phenolic content, while the ethyl acetate extract from the leaves yielded a high concentration of flavonoids. The ethyl acetate extract from the stems exhibited the highest activity in scavenging of 2,2-diphenyl- 1-picrylhydrazyl (DPPH), as well as in protecting erythrocytes. The leaves aqueous extract exhibited the highest ferrous chelating activity and its methanolic extract was found to be the strongest inhibitor of lipid peroxidation in β-carotene bleaching assay. The leaves chloroform extracts as well as the flowers methanol, chloroform, and ethyl acetate extracts were found to decrease the pro-inflammatory tumor necrosis factor alpha (TNF-α) cytokine levels in a dose-dependent manner. On the other hand, the flowers methanolic extract and the leaves methanol, ethyl acetate, and aqueous extracts decreased the interleukin-1 beta (IL- 1β) release. It was also found that the methanol extract from the flowers and the chloroform extract from the stems of Marrubium vulgare inhibited interleukin-8 (IL-8) release. This study provides a scientific basis for the traditional use of Marrubium vulgare as an anti-inflammatory agent and for the plant to be considered as an important resource of natural antioxidants.

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Recent Advances in the Development of Antimicrobial Peptides (AMPs): Attempts for Sustainable Medicine?

Current Medicinal Chemistry ◽

10.2174/0929867325666180117142142 ◽

2018 ◽

Vol 25 (21) ◽

pp. 2503-2519 ◽

Cited By ~ 4

Author(s):

Anne Kokel ◽

Marianna Torok

Keyword(s):

Side Effects ◽

Antimicrobial Peptides ◽

Potential Drug ◽

Green Technologies ◽

Specific Antibodies ◽

Structural Modifications ◽

Drug Candidates ◽

Induce Resistance ◽

Conventional Antibiotics

Background: Since the first isolation of antimicrobial peptides (AMPs) they have attracted extensive interest in medicinal chemistry. However, only a few AMP-based drugs are currently available on the market. Despite their effectiveness, biodegradability, and versatile mode of action that is less likely to induce resistance compared to conventional antibiotics, AMPs suffer from major issues that need to be addressed to broaden their use. Notably, AMPs can lack selectivity leading to side effects and cytotoxicity, and also exhibit in vivo instability. Several strategies are being actively considered to overcome the limitations that restrain the success of AMPs. Methods: In the current work, recent strategies reported for improving AMPs in the context of drug design and delivery were surveyed, and also their possible impact on patients and the environment was assessed. Results: As a major advantage AMPs possess an easily tunable skeleton offering opportunities to improve their properties. Strategic structural modifications and the beneficial properties of cyclic or branched AMPs in term of stability have been reported. The conjugation of AMPs with nanoparticles has also been explored to increase their in vivo stability. Other techniques such as the coupling of AMPs with specific antibodies aim to increase the selectivity of the potential drug towards the target. These strategies were evaluated for their effect on the environment highlighting green technologies. Conclusion: Although further research is needed taking into account both environmental and human health consequences of novel AMPs, several of these compounds are promising drug candidates for use in sustainable medicine.

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Verbascum sinaiticum: A Rich Source of Antioxidant Phenylethanoid Glycosides

The Natural Products Journal ◽

10.2174/2210315509666190129160405 ◽

2020 ◽

Vol 10 (2) ◽

pp. 158-162 ◽

Cited By ~ 1

Author(s):

Humaira Yasmeen Gondal ◽

Roshan Zamir ◽

Muhammad Nisar ◽

Muhammad Iqbal Choudhary

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Aerial Part ◽

Methanolic Extract ◽

Water Soluble ◽

Antioxidant Potential ◽

Active Fraction ◽

Phenylethanoid Glycosides ◽

Active Constituents ◽

Soluble Part

Background: The genus Verbascum is well documented for its antioxidant potential but Verbascum sinaiticum is comparatively less studied plant. The current study was carried out to search for antioxidant nutraceuticals from this species. Objective: To explore the antioxidant potential of Verbascum sinaiticum and to identify its active constituents. Methods: The methanolic extract of air-dried aerial part of the Verbascum sinaiticum was partitioned with hexane, chloroform and ethyl acetate. The water-soluble part of ethyl acetate afforded six phenylethanoid glycosides by repeated chromatography over Sephadex LH-20, silica gel and ODS columns. Antioxidant activity of solvent extracts and isolated constituents were evaluated by DPPH, ABTS and FRAP assays. Results: Six phenylethanoid glycosides was isolated and characterized as Verbascoside, Eukovoside, Martynoside, Jionoside D, Campneoside I and Campneoside II, from the most active fraction. Conclusion: Verbascum sinaiticum demonstrated prospective antioxidant activity. The watersoluble part of EtOAc (WSEAE) was found the most active extract whereas Verbascoside was identified as the most potent constituent. All isolated compounds exhibited significant antioxidant activity whereas their synergistic effect was found prominent in the parent fraction.

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Marine Natural Products, IX A New Halogenated Sesquiterpene Pinnatifidone from the Red Alga Laurencia pinnatifida

Zeitschrift für Naturforschung B ◽

10.1515/znb-1988-1022 ◽

1988 ◽

Vol 43 (10) ◽

pp. 1347-1350 ◽

Cited By ~ 7

Author(s):

Shaheen Bano ◽

Mohammad Shaiq Ali ◽

Viqar Uddin Ahmad

Keyword(s):

Natural Products ◽

Ethyl Acetate ◽

Marine Natural Products ◽

Methanolic Extract ◽

Ethyl Acetate Fraction ◽

Spectroscopic Studies ◽

Red Alga

Abstract From the ethyl acetate fraction of methanolic extract of red alga, L.pinnatifida, a new halogenated sesquiterpene named as pinnatifidone [1] has been isolated and the structure of this compound has been elucidated with the help of intensive spectroscopic studies.

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Phytochemicals as potential drug candidates for targeting SARS CoV 2 proteins, an in silico study

VirusDisease ◽

10.1007/s13337-021-00654-x ◽

2021 ◽

Author(s):

Anish Nag ◽

Ritesh Banerjee ◽

Rajshree Roy Chowdhury ◽

Chandana Krishnapura Venkatesh

Keyword(s):

In Silico ◽

Potential Drug ◽

Drug Candidates ◽

In Silico Study

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Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis

Parasites & Vectors ◽

10.1186/s13071-021-04693-7 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Olfa Chiboub ◽

Ines Sifaoui ◽

Manef Abderrabba ◽

Mondher Mejri ◽

José J. Fernández ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Cell Death ◽

Mitochondrial Membrane ◽

Brown Seaweed ◽

Cell Permeability ◽

Oxygen Species ◽

Potential Drug ◽

Concentration Cell ◽

Drug Candidates

Abstract Background The in vitro activity of the brown seaweed Dictyota spiralis against both Leishmania amazonensis and Trypanosoma cruzi was evaluated in a previous study. Processing by bio-guided fractionation resulted in the isolation of three active compounds, classified as diterpenes. In the present study, we performed several assays to detect clinical features associated to cell death in L. amazonensis and T. cruzi with the aim to elucidate the mechanism of action of these compounds on parasitic cells. Methods The aims of the experiments were to detect and evaluate specific events involved in apoptosis-like cell death in the kinetoplastid, including DNA condensation, accumulation of reactive oxygen species and changes in ATP concentration, cell permeability and mitochondrial membrane potential, respectively, in treated cells. Results The results demonstrated that the three isolated diterpenes could inhibit the tested parasites by inducing an apoptosis-like cell death. Conclusions These results encourage further investigation on the isolated compounds as potential drug candidates against both L. amazonensis and T. cruzi. Graphic abstract

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Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

Scientific Reports ◽

10.1038/s41598-021-87725-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Shabnam Javed ◽

Zaid Mahmood ◽

Khalid Mohammed Khan ◽

Satyajit D. Sarker ◽

Arshad Javaid ◽

...

Keyword(s):

Antifungal Activity ◽

Ethyl Acetate ◽

Oleanolic Acid ◽

Vanillic Acid ◽

Fungal Biomass ◽

Protocatechuic Acid ◽

Methanolic Extract ◽

Antifungal Compound ◽

Chloroform Fraction ◽

Lupeol Acetate

AbstractAntifungal activity of Monothecabuxifolia methanolic extract and its various fractions were assessed against Macrophominaphaseolina, a soil-borne fungal pathogen of more than 500 vegetal species as well as rare and emerging opportunistic human pathogen. Different concentrations of methanolic extract (3.125 to 200 mg mL−1) inhibited fungal biomass by 39–45%. Isolated n-hexane, chloroform and ethyl acetate fractions suppressed fungal biomass by 32–52%, 29–50% and 29–35%, respectively. Triterpenes lupeol and lupeol acetate (1, 2) were isolated from n-hexane while betulin, β-sitosterol, β-amyrin, oleanolic acid (3–6) were isolated from chloroform fraction. Vanillic acid, protocatechuic acid, kaempferol and quercetin (7–10) were isolated from the ethyl acetate fraction and identified using various spectroscopic techniques namely mass spectroscopy and NMR. Antifungal activity of different concentrations (0.0312 to 2 mg mL−1) of the isolated compounds was evaluated and compared with the activity of a broad spectrum fungicide mancozeb. Different concentrations of mencozeb reduced fungal biomass by 83–85%. Among the isolated compounds lupeol acetate (2) was found the highest antifungal against M.phaseolina followed by betulin (3), vanillic acid (7), protocatechuic acid (8), β-amyrin (5) and oleanolic acid (6) resulting in 79–81%, 77–79%, 74–79%, 67–72%, 68–71% and 68–71%, respectively. Rest of the compounds also showed considerable antifungal activity and reduced M.phaseolina biomass by 41–64%.

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