scholarly journals Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of Rosa acicularis and the Main Ellagitannin Rugosin D

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2525
Author(s):  
Daniil N. Olennikov ◽  
Vladimir V. Chemposov ◽  
Nadezhda K. Chirikova
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Leaf Extract ◽  
Digestive Enzyme ◽  
Flavonoid Glycosides ◽  
Digestion Method ◽  
Photodiode Array Detection ◽  
Great Ability ◽  
Simulated Digestion ◽  
Main Component

Prickly rose (Rosa acicularis Lindl.) is the most distributed rose species in the Northern Hemisphere, used by indigenous people for various food purposes. The lack of detailed information about the chemical composition of R. acicularis has led us to study the phytochemical composition and metabolic profile of prickly rose extracts using chromatographic techniques. Many groups of phenolic and non-phenolic compounds were quantified in the leaves, flowers, roots and fruits of R. acicularis. Phenolic compounds were the dominant phytochemicals in the aerial parts and roots of R. acicularis. A precise study by high-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass spectrometric detection showed the presence of 123 compounds, among which ellagic acid derivatives, ellagitannins, gallotannins, catechins, catechin oligomers, hydroxycinnamates and flavonoid glycosides of kaempferol, quercetin and dihydroquercetin were all identified for the first time. The most abundant phenolic compounds were ellagitannins and flavonoid glycosides, with a maximal content of 70.04 mg/g in leaves and 66.72 mg/g in flowers, respectively, indicating the great ability of R. acicularis organs to accumulate phenolic compounds. By applying a standardized static, simulated gastrointestinal digestion method, we found the inhibitory potential of the leaf extract against digestive α-amylases. A pancreatic α-amylase activity-inhibiting assay coupled with HPLC microfractionation demonstrated high inhibition of enzyme activity by ellagitannin rugosin D, which was later confirmed by a microplate reaction with mammalian α-amylases and the simulated digestion method. This study clearly demonstrates that R. acicularis leaf extract and its main component, ellagitannin rugosin D, strongly inhibit digestive α-amylase, and may be a prospective antidiabetic agent.

scholarly journals Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 783 ◽  
Author(s):  
Mirjana Mosić ◽  
Jelena Trifković ◽  
Irena Vovk ◽  
Uroš Gašić ◽  
Živoslav Tešić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Ion Trap ◽  
Therapeutic Potential ◽  
Flavonoid Glycosides ◽  
Phenolic Composition ◽  
Bee Pollen ◽  
Orbitrap Mass Spectrometry ◽  
Health Promoting ◽  
First Time

Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.

scholarly journals Sustainable Micro-Scale Extraction of Bioactive Phenolic Compounds from Vitis vinifera Leaves with Ionic Liquid-Based Surfactants

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3072 ◽  
Author(s):  
Giulia Mastellone ◽  
Idaira Pacheco-Fernández ◽  
Patrizia Rubiolo ◽  
Verónica Pino ◽  
Cecilia Cagliero
Keyword(s):  
Ionic Liquid ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
High Performance ◽  
Photodiode Array Detection ◽  
Micro Scale ◽  
Simple Strategy ◽  
Analytical System ◽  
Solid Liquid ◽  
Extraction Phase

This paper proposes a new sustainable and simple strategy for the micro-scale extraction of phenolic compounds from grapevine leaves with analytical purpose. The method is based on a microwave-assisted solid-liquid extraction approach (MA-SLE), using an aqueous solution of an ionic liquid (IL)-based surfactant as extraction phase. The method does not require organic solvents, nor any clean-up step, apart from filtration prior to the injection in the analytical system. Two IL-based surfactants were evaluated, and the method was optimized by using experimental designs, resulting in the use of small amounts of sample (100 mg) and extraction phase (2.25 mL), low concentrations of the selected 1-hexadecyl-3-butyl imidazolium bromide IL (0.1 mM), and 30 min of extraction time. The proposed methodology was applied for the determination of the polyphenolic pattern of six different varieties of Vitis vinifera leaves from the Canary Islands, using high-performance liquid chromatography and photodiode array detection for the quantification of the compounds. The proposed MA-SLE approach was greener, simpler, and more effective than other methods, while the results from the analysis of the leaves samples demonstrate that these by-products can be exploited as a source of natural compounds for many applications.

Phytochemical Fingerprints of Lime Honey Collected in Serbia

2014 ◽  
Vol 97 (5) ◽  
pp. 1259-1267 ◽  
Author(s):  
Uroš Gašić ◽  
Branko Šikoparija ◽  
Tomislav Tosti ◽  
Jelena Trifković ◽  
Dušanka Milojković-Opsenica ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Sugar Content ◽  
Amperometric Detection ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Flavonoid Glycosides ◽  
Lime Tree ◽  
Hybrid Mass Spectrometer ◽  
Production Stages

Abstract Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia. Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar. Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples. Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC–OrbiTrap). Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection. Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences. Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey. Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation. Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples. Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.

scholarly journals LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1770
Author(s):  
Akhtar Ali ◽  
Yasmeen M. Bashmil ◽  
Jeremy J. Cottrell ◽  
Hafiz A. R. Suleria ◽  
Frank R. Dunshea
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Positive Impact ◽  
Total Phenolic ◽  
Fragmentation Pattern ◽  
Photodiode Array Detection ◽  
Potential Health

Culinary spices and herbs have been used to impart a characteristic flavour and aroma in food due to their appealing fragrance. Recently, bioactive compounds from herbs, especially phenolics, have gained much attention due to their potential health outcomes. The aim of this study was to characterize and quantify the phenolic compounds from 10 widely used Australian-grown herbs (oregano, rosemary, bay, basil, sage, fenugreek, dill, parsley, mint and thyme). For this purpose, liquid chromatography mass spectrometry (LC-MS) was used for the complete profiling of polyphenolic compounds and quantification of abundant phenolic compounds was completed with high-performance liquid chromatography—photodiode array detection (HPLC-PDA). Polyphenols from Australian-grown herbs were estimated through total phenolic content (TP), total flavonoids (TF) and total tannins (TT) along with their in-vitro antioxidant activities. Oregano and mint were estimated with the highest value of TP (140.59 ± 9.52 and 103.28 ± 8.08 mg GAE/g, milligram gallic acid equivalent/gram) while rosemary and mint had the highest TF (8.19 ± 0.74 and 7.05 ± 0.43 mg QE (quercetin equivalent)/g). In this study, eighty-four (84) phenolic compounds were screened and confirmed through LC-MS/MS by comparing their masses and fragmentation pattern with published libraries. The results of this study validate the use of these herbs as bioactives and their positive impact on human health.

scholarly journals HPLC-DAD-ESI-MSn identification of phenolic compounds in cultivated strawberries from Macedonia

2010 ◽  
Vol 29 (2) ◽  
pp. 181 ◽  
Author(s):  
Marina Kajdžanoska ◽  
Viktor Gjamovski ◽  
Marina Stefova
Keyword(s):  
Phenolic Compounds ◽  
Ellagic Acid ◽  
High Performance ◽  
Negative Ion ◽  
Ionization Mass ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection ◽  
Strawberry Cultivar ◽  
Negative Ion Mode

Strawberry fruits contain phenolic compounds that exhibit antioxidant, anticancer, antiatherosclerotic, antinflammatory and anti-neurodegenerative properties. High-performance liquid chromatography (HPLC) coupled to electrospray ionization mass spectrometric (ESI-MS) detection in the positive and negative ion mode has been used to identify the phenolic compounds in extracts from sixteen different strawberry cultivar fruits from Republic of Macedonia. Photodiode-array detection (DAD) has been used for screening of the different classes of phenolic compounds, whereas MS and MSn fragmentation data were employed for their structural characterization. The phenolic compounds identified were grouped as: ellagic acid and ellagic acid conjugates with sugars, ellagitannins, anthocyanins, flavonols, flavanols, and acylated sugars (feruloyl, caffeoyl and coumaroyl hexoses). Quercetin and kaempferol were the major flavonols found as quercetin 3-O-glucoside, quercetin 3-O-glucuronide, kaempferol 3-O-glucoside, kaempferol 3-O-glucoronide, kaempferol 3-O-malonylglucoside, kaempferol 3-O-acetylglucoside and kaempferol 3-O-coumaroylglucoside. Pelargonidin-3-O-glucoside was the most abundant anthocyanin in all strawberry extracts. Proanthocyanidins were also identified by MSn fragmentation as dimers, trimers and tetramers of (epi)catechin and (epi)afzelechin. This is the first assay of the phenolic profile of the strawberry cultivars in Macedonia, which can be further developed for characterization and evaluation of their quality with regards to their phenolic composition.

scholarly journals Determination of phenolic compounds in water-ethanol extracts of Populus tremula L. leaves using high-performance liquid chromatography

2020 ◽  
Vol 10 (3) ◽  
pp. 470-478
Author(s):  
O. V. Kotsupiy ◽  
Yu. V. Zagurskaya ◽  
V. I. Ufimtsev
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Solid Phase Extraction ◽  
High Performance ◽  
Solid Phase ◽  
Membrane Filter ◽  
Flavonoid Glycosides ◽  
Phase Extraction ◽  
Ethanol Extracts

The analytical task of determining the phenolic compound content of water-ethanol extracts of Populus tremula L. (common aspen) leaves is complicated by the heterogeneity of compound groups having different polarities and appearing in varying concentrations. The purpose of the present work is to study the conditions of solid-phase extraction and high-performance liquid chromatography used to analyse the content of different groups of phenolic compounds in water-ethanol extracts of leaves from the P. tremula plant. In order to facilitate the derivation of phenolic compounds, an exhaustive extraction process was carried out using ethanol. Solid-phase extraction was carried out using a Diapak C16 cartridge, after which the eluates were passed through a membrane filter having a pore diameter of 0.45 μm. The high-performance liquid chromatography method was used to determine the content of phenolic acids and flavonoid glycosides, as well as salicin and individual flavonoid glycoside components: hyperoside, rutin, astragalin and two unidentified flavonoid glycosides in aqueous (analyte 1) and aqueous-alcoholic fractions (analyte 2) in two systems along the gradient elution. The requirement of analysing the primary aqueous eluate together or in parallel with the main aqueous-alcoholic fraction in the preparation of P. tremula leaf extracts for high-performance liquid chromatography using solid-phase extraction cartridges was substantiated. For separating the extract to determine the hydroxycinnamic and hydroxybenzoic acid content, it is preferable to use system 2; for determining the phenologlycoside (salicin) content, system 1 is more effective. Flavonoid glycosides (hyperoside, rutin, astragalin and two unidentified flavonoids) make the most significant contribution to the difference between the aqueous and aqueous-alcoholic fractions.

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