scholarly journals Genotypic Differences in the Effect of P Fertilization on Phytic Acid Content in Rice Grain

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 146 ◽  
Author(s):  
Ayaka Fukushima ◽  
Ishara Perera ◽  
Koki Hosoya ◽  
Tatsuki Akabane ◽  
Naoki Hirotsu
Keyword(s):  
Gene Expression ◽  
Phytic Acid ◽  
Xylem Sap ◽  
Genotypic Difference ◽  
Genotypic Differences ◽  
Rice Grain ◽  
P Fertilization ◽  
Phytic Acid Content ◽  
Inorganic P ◽  
P Content

Phytic acid (PA) prevents the absorption of minerals in the human intestine, and it is regarded as an antinutrient. Low PA rice is beneficial because of its higher Zn bioavailability and it is suggested that the gene expression level of myo-inositol 3-phosphate synthase 1 (INO1) in developing grain is a key factor to explain the genotypic difference in PA accumulation among natural variants of rice. P fertilization is also considered to affect the PA content, but it is not clear how it affects INO1 gene expression and the PA content in different genotypes. Here, we investigated the effect of P fertilization on the PA content in two contrasting rice genotypes, with low and high PA accumulation, respectively. Based on the results of the analysis of the PA content, inorganic P content, INO1 gene expression, and xylem sap inorganic P content, we concluded that the effect of P fertilization on PA accumulation in grain differed with the genotype, and it was regulated by multiple mechanisms.

scholarly journals Mutation of Inositol 1,3,4-trisphosphate 5/6-kinase6 Impairs Plant Growth and Phytic Acid Synthesis in Rice

Plants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 114 ◽  
Author(s):  
Meng Jiang ◽  
Yang Liu ◽  
Yanhua Liu ◽  
Yuanyuan Tan ◽  
Jianzhong Huang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Phytic Acid ◽  
Targeted Mutagenesis ◽  
Rice Grain ◽  
Nucleotide Substitutions ◽  
Phytic Acid Content ◽  
Frameshift Mutations ◽  
A Genome ◽  
Mutant Lines ◽  
Growth And Reproduction

Inositol 1,3,4-trisphosphate 5/6-kinase (ITPK) is encoded by six genes in rice (OsITPK1-6). A previous study had shown that nucleotide substitutions of OsITPK6 could significantly lower the phytic acid content in rice grains. In the present study, the possibility of establishing a genome editing-based method for breeding low-phytic acid cultivars in rice was explored, in conjunction with the functional determination of OsITPK6. Four OsITPK6 mutant lines were generated by targeted mutagenesis of the gene’s first exon using the CRISPR/Cas9 method, one (ositpk6_1) with a 6-bp in-frame deletion, and other three with frameshift mutations (ositpk6_2, _3, and _4). The frameshift mutations severely impaired plant growth and reproduction, while the effect of ositpk6_1 was relatively limited. The mutant lines ositpk6_1 and _2 had significantly lower levels (−10.1% and −32.1%) of phytic acid and higher levels (4.12- and 5.18-fold) of inorganic phosphorus compared with the wild-type (WT) line. The line ositpk6_1 also showed less tolerance to osmotic stresses. Our research demonstrates that mutations of OsITPK6, while effectively reducing phytic acid biosynthesis in rice grain, could significantly impair plant growth and reproduction.

scholarly journals Combination of High Zn Density and Low Phytic Acid for Improving Zn Bioavailability in Rice (Oryza stavia L.) Grain

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yin Wang ◽  
Yusha Meng ◽  
Yanping Ma ◽  
Lei Liu ◽  
Dianxing Wu ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Double Mutant ◽  
Cell Model ◽  
Brown Rice ◽  
Molar Ratio ◽  
Zn Deficiency ◽  
Rice Grain ◽  
Phytic Acid Content ◽  
Low Phytic Acid ◽  
Zn Bioavailability

Abstract Background Zn deficiency is one of the leading public health problems in the world. Staple food crop, such as rice, cannot provide enough Zn to meet the daily dietary requirement because Zn in grain would chelate with phytic acid, which resulted in low Zn bioavailability. Breeding new rice varieties with high Zn bioavailability will be an effective, economic and sustainable strategy to alleviate human Zn deficiency. Results The high Zn density mutant LLZ was crossed with the low phytic acid mutant Os-lpa-XS110–1, and the contents of Zn and phytic acid in the brown rice were determined for the resulting progenies grown at different sites. Among the hybrid progenies, the double mutant always displayed significantly higher Zn content and lower phytic acid content in grain, leading to the lowest molar ratio of phytic acid to Zn under all environments. As assessed by in vitro digestion/Caco-2 cell model, the double mutant contained the relatively high content of bioavailable Zn in brown rice. Conclusions Our findings suggested pyramiding breeding by a combination of high Zn density and low phytic acid is a practical and useful approach to improve Zn bioavailability in rice grain.

scholarly journals Genotypic Variation in Spatial Distribution of Fe in Rice Grains in Relation to Phytic Acid Content and Ferritin Gene Expression

Rice Science ◽  
2020 ◽  
Vol 27 (3) ◽  
pp. 227-236
Author(s):  
Anurag Mishra ◽  
Md. Shamim ◽  
Md. Wasim Siddiqui ◽  
Akanksha Singh ◽  
Deepti Srivastava ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spatial Distribution ◽  
Phytic Acid ◽  
Acid Content ◽  
Genotypic Variation ◽  
Phytic Acid Content ◽  
Rice Grains ◽  
Ferritin Gene

scholarly journals Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ishara Perera ◽  
Ayaka Fukushima ◽  
Tatsuki Akabane ◽  
Genki Horiguchi ◽  
Saman Seneweera ◽  
...  
Keyword(s):  
Gene Expression ◽  
Phytic Acid ◽  
Reverse Genetics ◽  
Developmental Stages ◽  
Rice Grain ◽  
Genetic Determinants ◽  
Genome Wide ◽  
Significant Single Nucleotide Polymorphism ◽  
Early Developmental ◽  
Phosphate Synthase

Abstract Phytic acid (PA) is the primary phosphorus (P) storage compound in the seeds of cereals and legumes. Low PA crops, which are considered an effective way to improve grain nutrient availability and combat environmental issues relating to seed P have been developed using mutational and reverse genetics approaches. Here, we identify molecular mechanism regulating PA content among natural rice variants. First, we performed genome-wide association (GWA) mapping of world rice core collection (WRC) accessions to understand the genetic determinants underlying PA trait in rice. Further, a comparative study was undertaken to identify the differences in PA accumulation, protein profiles, and gene expression in low (WRC 5) and high PA (WRC 6) accessions. GWA results identified myo-inositol 3-phosphate synthase 1 (INO1) as being closely localized to a significant single nucleotide polymorphism. We found high rates of PA accumulation 10 days after flowering, and our results indicate that INO1 expression was significantly higher in WRC 6 than in WRC 5. Seed proteome assays found that the expression of INO1 was significantly higher in WRC 6. These results suggest that not only the gene itself but regulation of INO1 gene expression at early developmental stages is important in determining PA content in rice.

scholarly journals Effect of rice variety and blending proportion on the proximate compositions, minerals and phytic acid contents of bread from rice-teff blend

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 116
Author(s):  
Sintayehu Legesse ◽  
Solomon Worku ◽  
Geremew Bultosa
Keyword(s):  
Phytic Acid ◽  
Rice Variety ◽  
Chemical Properties ◽  
Zinc Content ◽  
Eragrostis Tef ◽  
Rice Grain ◽  
Potential Health ◽  
Phytic Acid Content ◽  
Custom Design ◽  
Optimal Value

Development of bakery products containing rice (Oryza sativa, Linn.) and teff (Eragrostis tef) could have potential health benefits due to their gluten free nature. Nine experimental runs were generated using custom design by JMP 8 software. The effect of two factors, rice variety (Edeget, X-jigna and Nerica-4) and blending proportions of rice and teff (0.5:0.5, 0.7:0.3 and 0.9:0.1) were studied. The data analysis was conducted using SAS software package for the mean comparison and custom design by JMP 8 software. Response surface methodology was applied to study the interaction effect of the main factors and to generate the predictive equations. An optimal value (1.60%) of fiber was obtained when the proportion of the blend was 50% Edeget and 50% teff because teff grain is high in fiber. A maximum value (10.75%) of protein was obtained when the proportion of the blend was 70% Nerica-4 and 30% teff. Carbohydrate was optimal (81.37%) when 90% Edeget and 10% teff were blended because rice grain is high in carbohydrate. Optimal iron content (12.97 mg/100g) was obtained when the proportion of the blend was 50% Nerica-4 and 50% teff because teff grain is high in iron. Optimal zinc content (4.14 mg/100g) was obtained when the proportion of the blend was 50% X-jigna and 50% teff. The optimal value (61.25 mg/100g) of calcium was obtained when the proportion of the blend was 50% Edeget and 50% teff.  Optimum (lower) value (0.31mg/g) of phytic acid was obtained when the proportion of the blend was 90% Nerica-4 and 10% teff because rice grain is lower in phytic acid content. It was concluded that rice variety and rice-teff blending proportion had a significant effect on the physico-chemical properties of rice-teff blend bread. An optimal nutrient blend (high in nutrients, low in anti-nutrients) was obtained when 70% Edeget rice variety was blended with 30% teff. All the derived mathematical models for the various responses were found to fit significantly to the predicted data.

Bioavailability of iron and zinc as affected by phytic acid content in rice grain

2017 ◽  
Vol 41 (6) ◽  
pp. e12413 ◽  
Author(s):  
Awadhesh Kumar ◽  
Milan Kumar Lal ◽  
Subhra Sashi Kar ◽  
Lopamudra Nayak ◽  
Umakanta Ngangkham ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Acid Content ◽  
Rice Grain ◽  
Phytic Acid Content ◽  
Iron And Zinc

scholarly journals Phosphorus bioavailability in soybean grown after pasture under different fertility regimes

2017 ◽  
Vol 38 (2) ◽  
pp. 571
Author(s):  
Diogo Néia Eberhardt ◽  
Thierry Becquer ◽  
Robélio Leandro Marchão ◽  
Pedro Rodolfo Vendrame ◽  
Lourival Vilela ◽  
...  
Keyword(s):  
Dry Matter ◽  
Residual Effect ◽  
P Fertilization ◽  
Inorganic P ◽  
Residual P ◽  
P Content ◽  
P Fertilizer ◽  
Urochloa Decumbens ◽  
Pasture Fertilization ◽  
P Bioavailability

The aim of this study was to evaluate the residual effect of phosphorus (P) fertilizer applied to an Urochloa decumbens cv. Basilisk pasture on the P bioavailability to the following soybean crop. Low-productivity pasture, planted on an Oxisol in an experimental field at Embrapa Cerrados, was divided into three strips, each of 1.5 ha and fertilized by broadcasting annual applications of 0, 20 and 40 kg ha-1 of P2O5 for four years. After the pasture was desiccate with herbicide, soybeans were sown and fertilized with 0, 50 and 100 kg ha-1 of P2O5 applied within each strip, making a total of nine (3x3) P treatments. Residual available P content (Mehlich-1 and resin) from the pasture fertilization was lower than from soybean fertilization. However, the bioavailable residual P from the pasture, determined by P accumulated in plants, production of dry matter and grain yield, had similar availability to inorganic P applied to the soybean. Early P fertilization applied to pasture is technically feasible and can be used to maintain the pasture: it is recommended to replace corrective fertilization for following soybean crops.

Effect of Fermentation Methods on Phytic acid Content in Wheat Flour Bread

2014 ◽  
Vol 27 (1) ◽  
pp. 128-142
Author(s):  
Ali A. Sahi ◽  
Ali H. Abdul-Kareem ◽  
Basim A. Jaber
Keyword(s):  
Phytic Acid ◽  
Wheat Flour ◽  
Acid Content ◽  
Phytic Acid Content

Respon Morfologis dan Ekspresi Gen Aquaporin pada Padi IR 64 yang Mengalami Cekaman Kekeringan pada Fase Reproduktif (Morphological Responses and Aquaporin Gene Expression in Rice IR64 under Drought Stress at the Reproductive Stage)

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Made Pharmawati ◽  
Ni Nyoman Wirasiti ◽  
Luh Putu Wrasiati
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Real Time ◽  
Real Time Pcr ◽  
Control Plant ◽  
Reproductive Stage ◽  
Limiting Factors ◽  
Rice Grain ◽  
Aquaporin Gene ◽  
Peg Treatment

Abstrak Cekaman kekeringan merupakan faktor pembatas penting bagi pertumbuhan dan produktivitas tanaman termasuk padi.      Penelitian ini bertujuan menganalisis respon padi IR64 terhadap cekaman kekeringan dengan pemberian polietilen glikol (PEG) pada fase reproduktif.  Penelitian juga bertujuan menganalisis ekspresi gen aquaporin akibat cekaman kekeringan.  Bibit padi ditanam dalam pot dan perlakuan PEG dengan konsentrasi 108g/L (-0.25MPa) dan 178g/L (-0.52 MPa) diberikan saat munculnya panikula. Perlakuan diberikan selama 2 minggu, kemudian tanaman disiram kembali.  Ekspresi gen diamati pada akhir perlakuan dengan semi kuantitatif real time PCR.  Ekstraksi RNA menggunakan RNeasy plant mini kit, sedangkan sintesis cDNA menggunakan Transcriptor First Strand cDNA Kit.  Hasil penelitian menunjukkan bahwa jumlah malai dan berat total malai berkurang akibat cekaman kekeringan.  Persentase gabah kosong mencapai 84,6% pada perlakuan PEG-0,52 MPa, sedangkan pada perlakuan PEG -0,25 MPa persentase gabah kosong sebesar 67,8%.  Pada kontrol persentase gabah kosong adalah 10,3%.  Ekspresi gen OsPIP2;7 sedikit menurun pada perlakuan PEG -0,52 MPa.Kata kunci: ekspresi gen, IR64, kekeringan, padi, PEG  Abstract Drought stress is one of the limiting factors of plant growth and productivity including rice.  The aim of this study was to analyze responses of IR64 rice to polyethylene glycol (PEG)-induced-drought stress at the reproductive stage.  This study also aimed to analyze the expression of aquaporin under drought stress.  Rice seedlings were grown in pot system and PEG treatment at concentration of -0.25MPa (108g/L) and -0.52 MPa (178g/L) were given when the panicles arose.  Treatments were conducted for 2 weeks, after that the plants were rewatered.  Gene expression was evaluated at the end of PEG treatment using semi quantitative real time PCR. RNA was extracted using RNeasy plant mini kit, while cDNA synthesis was done using Transcriptor First Strand cDNA Kit.  The results showed that the number and weight of rice ear were less in plant treated with PEG than in control.  The percentage of empty rice grain reached 84.6% at PEG -0.52 MPa, while at PEG -0.25 MPa the percentage of empty grain was 67.8%.  In control plant, the percentage of empty grain was 10.3%.  Drought stress did not alter the expression of OsPIP2;7.  Keywords: drought, gene expression, IR64, PEG, rice

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