scholarly journals Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1517 ◽  
Author(s):  
Lenka Vítková ◽  
Lenka Musilová ◽  
Eva Achbergerová ◽  
Antonín Minařík ◽  
Petr Smolka ◽  
...  
Keyword(s):  
Polyethylene Oxide ◽  
Average Molecular Weight ◽  
Polymer Concentration ◽  
Sodium Hyaluronate ◽  
Electrospinning Process ◽  
Poly Vinyl Alcohol ◽  
Solvent Mixtures ◽  
Fluorescence Confocal Microscopy ◽  
Dc Electric Field ◽  
Solution Surface

In the current study, we present methods of sodium hyaluronate, also denoted as hyaluronan (HA), nanofiber fabrication using a direct-current (DC) electric field. HA was spun in combination with poly(vinyl alcohol) (PVA) and polyethylene oxide (PEO) and as a pure polymer. Nonaggressive solvents were used due to the possible use of the fibers in life sciences. The influences of polymer concentration, average molecular weight (Mw), viscosity, and solution surface tension were analyzed. HA and PVA were fluorescent-labeled in order to examine the electrospun structures using fluorescence confocal microscopy. In this study, two intermediate solvent mixtures that facilitate HA electrospinning were found. In the case of polymer co-electrospinning, the effect of the surfactant content on the HA/PVA electrospinning process, and the effect of HA Mw on HA/PEO nanofiber morphology, were examined, respectively.

scholarly journals Electrospinning Nanofibres Of Pullulan Extracted From Phylloplane Fungus, Aureobasidium Pullulans

2020 ◽  
Author(s):  
Komal Saraf ◽  
N Vigneshwaran
Keyword(s):  
Surface Area ◽  
Aureobasidium Pullulans ◽  
High Surface ◽  
Polymer Concentration ◽  
Carbon Sources ◽  
High Surface Area ◽  
Angle Measurement ◽  
Electrospinning Process ◽  
Bet Surface Area ◽  
Poly Vinyl Alcohol

Abstract Aureobasidium pullulans isolated from the phylloplane of Peltophorum tree, produced pullulan, an extracellular polysaccharide. It was grown on three different carbon sources, sucrose, wheat bran and cotton stalk dust, for maximizing the pullulan yield. A. pullulans (67.4 gL-1) had the highest yield followed by A. pullulans MTCC 1991 (63.68 gL-1). Pullulan was characterized by X-ray diffractometer (XRD), Brunauer-Emmett-Teller (BET) surface area analyzer, DSC and NMR. Electrospinning of pullulan blended with poly (vinyl alcohol) (PVA) produced bead-less nanofibres. The optimized parameters for electrospinning were 25 kV applied voltage, 0.5 mL/h flow rate, 18% polymer concentration (pullulan + PVA) and 150 mm tip-to-collector distance. The pullulan nanofibre was characterized by SEM, AFM, BET, contact angle measurement, DSC and CIE color space analyzer. A maximum surface area of 183.4 m2/g while the minimum nanofibre diameter (79 ± 19 nm by SEM) was obtained for the electrospun mat of commercial pullulan + 40% PVA. This work signifies the importance of pullulan extracted from an isolate of Peltopohorum tree for conversion to high surface area nanofibres by electrospinning process.

scholarly journals Electrospinning Nanofibres of Pullulan Extracted From Phylloplane Fungus, Aureobasidium Pullulans

2020 ◽  
Author(s):  
Komal Saraf ◽  
N Vigneshwaran
Keyword(s):  
Surface Area ◽  
Aureobasidium Pullulans ◽  
High Surface ◽  
Polymer Concentration ◽  
Carbon Sources ◽  
High Surface Area ◽  
Angle Measurement ◽  
Electrospinning Process ◽  
Bet Surface Area ◽  
Poly Vinyl Alcohol

Abstract Aureobasidium pullulans isolated from the phylloplane of Peltophorum tree, produced pullulan, 24 an extracellular polysaccharide. It was grown on three different carbon sources, sucrose, wheat 25 bran and cotton stalk dust, for maximizing the pullulan yield. A. pullulans (67.4 gL-1) had the 26 highest yield followed by A. pullulans MTCC 1991 (63.68 gL-1). Pullulan was characterized by 27 X-ray diffractometer (XRD), Brunauer-Emmett-Teller (BET) surface area analyzer, DSC and NMR. 28 Electrospinning of pullulan blended with poly (vinyl alcohol) (PVA) produced bead-less 29 nanofibres. The optimized parameters for electrospinning were 25 kV applied voltage, 0.5 mL/h 30 flow rate, 18% polymer concentration (pullulan + PVA) and 150 mm tip-to-collector distance. 31 The pullulan nanofibre was characterized by SEM, AFM, BET, contact angle measurement, DSC 32 and CIE color space analyzer. A maximum surface area of 183.4 m2/g while the minimum 33 nanofibre diameter (79 ± 19 nm by SEM) was obtained for the electrospun mat of commercial 34 pullulan + 40% PVA. This work signifies the importance of pullulan extracted from an isolate of 35 Peltopohorum tree for conversion to high surface area nanofibres by electrospinning process.

scholarly journals Design of Mucoadhesive Strips for Buccal Fast Release of Tramadol

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1187
Author(s):  
Nayla Francine Garcia Pastório ◽  
Camila Felix Vecchi ◽  
Rafaela Said dos Santos ◽  
Marcos Luciano Bruschi
Keyword(s):  
Ex Vivo ◽  
Polymer Concentration ◽  
Peroral Administration ◽  
Patient Acceptance ◽  
Poly Vinyl Alcohol ◽  
Synthetic Analogue ◽  
Drug Release Profile ◽  
Opioid Agonist ◽  
In Vivo Evaluation ◽  
Tramadol Hydrochloride

Tramadol hydrochloride is a synthetic analogue of codeine and shows activity on the central nervous system as an opioid agonist and inhibitor of serotonin and norepinephrine reuptake. It has been used for controlling moderate to severe pain. Mucoadhesive fast-dissolving films can present greater drug availability and patient acceptance when compared to the systems of peroral administration. The films were prepared using the solvent casting method with ethylcellulose, polyvinylpyrrolidone and poly(vinyl alcohol). The effect of each polymer concentration was investigated using a 2³ factorial design with repetition at the central point. The formulations were subjected to physicochemical, mechanical, ex vivo mucoadhesive and in vitro drug release profile analysis. These properties were dependent on the polymeric composition (independent factors) of each system. The optimized formulations showed good macroscopic characteristics, improved resistance to bending, rigidity, rapid swelling up to 60 s, improved mechanical and mucoadhesive characteristics, and also fast dissolving and tramadol release. The optimized formulations constitute platforms and strategies to improve the therapy of tramadol with regard to availability at the site of application, considering the necessity of rapid pain relief, and show potential for in vivo evaluation.

The electrospinning behavior of poly(vinyl alcohol) in DMSO–water binary solvent mixtures

RSC Advances ◽  
2016 ◽  
Vol 6 (105) ◽  
pp. 102947-102955 ◽  
Author(s):  
Deepika Gupta ◽  
Manjeet Jassal ◽  
Ashwini K. Agrawal
Keyword(s):  
Ternary System ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Binary Solvent ◽  
Solvent Mixtures ◽  
Binary Solvent Mixtures ◽  
Polymer Interactions ◽  
Solvent Solvent

The fiber diameters obtained from PVA–DMSO–water ternary system are the result of the interplay between the solvent–solvent and solvent–polymer interactions.

scholarly journals Development and Characterization of pH-Dependent Cellulose Acetate Phthalate Nanofibers by Electrospinning Technique

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3202
Author(s):  
Gustavo Vidal-Romero ◽  
Virginia Rocha-Pérez ◽  
María L. Zambrano-Zaragoza ◽  
Alicia Del Real ◽  
Lizbeth Martínez-Acevedo ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Polymer Concentration ◽  
Process Efficiency ◽  
Fickian Diffusion ◽  
Solvent Mixtures ◽  
Cellulose Acetate Phthalate ◽  
Scanning Calorimetry ◽  
Electrospinning Technique ◽  
Ph Dependent ◽  
Release Profiles

The aim of this work was to obtain pH-dependent nanofibers with an electrospinning technique as a novel controlled release system for the treatment of periodontal disease (PD). Cellulose acetate phthalate (CAP) was selected as a pH-sensitive and antimicrobial polymer. The NF was optimized according to polymeric dispersion variables, polymer, and drug concentration, and characterized considering morphology, diameter, entrapment efficiency (EE), process efficiency (PE), thermal properties, and release profiles. Two solvent mixtures were tested, and CHX-CAP-NF prepared with acetone/ethanol at 12% w/v of the polymer showed a diameter size of 934 nm, a uniform morphology with 42% of EE, and 55% of PE. Meanwhile, CHX-CAP-NF prepared with acetone/methanol at 11% w/v of polymer had a diameter of 257 nm, discontinuous nanofiber morphology with 32% of EE, and 40% of PE. EE and PE were dependent on the polymer concentration and the drug used in the formulation. Studies of differential scanning calorimetry (DSC) showed that the drug was dispersed in the NF matrix. The release profiles of CHX from CHX-CAP-NF followed Fickian diffusion dependent on time (t0.43−0.45), suggesting a diffusion–erosion process and a matrix behavior. The NF developed could be employed as a novel drug delivery system in PD.

scholarly journals Optimization of The Electrospinning Process for Preparation of Nanofibers From Poly (Vinyl Alcohol) (PVA) and Chromolaena odorata L. Extrac (COE)

2020 ◽  
Vol 16 (1) ◽  
pp. 47-56
Author(s):  
I. Sriyanti ◽  
L. Marlina ◽  
J. Jauhari
Keyword(s):  
Wound Dressing ◽  
Fiber Diameter ◽  
Sem Analysis ◽  
Electrospinning Process ◽  
The Body ◽  
Poly Vinyl Alcohol ◽  
Therapeutic Effects ◽  
Important Process ◽  
Chromolaena Odorata ◽  
Solubility In Water

The Cromaloena odorata (COE) contains phenols, flavonoids, tannins, alkaloids, saponins, steroids that possess diverse therapeutic effects. However, COE has poor solubility in water and poor absorbtion in the body. Incorporation of COE in nanofiber system is a promising way to increase CEO solubility. One of the method to produce nanofiber is electrospinning. The electrospinning process there are three of the most important process parameters are applied flowrate, voltage and TCD. In this study we developed optimized condition for electrospinning process of polyvinyl alcohol (PVA)/CEO and their characterization. The Scanning electron microscopy (SEM) analysis showed that modification of flowrate and TCD did not affect the morphology of PVA and COE fiber. However fiber diameter decreased when lower flowrate, higher voltage was applied, and TCD. Fourier Transform Infrared (FTIR) study was conducted to identify possible intermolecular interaction between PVA/COE that has potential application as antimicrobial wound dressing.

Synthesis of Catechin-Gelatin Nanofiber by Electrospinning

2017 ◽  
Vol 887 ◽  
pp. 96-99 ◽  
Author(s):  
Muhamad Nasir ◽  
Dita Apriani
Keyword(s):  
Polyethylene Oxide ◽  
Chemical Structure ◽  
Sem Analysis ◽  
Electrospinning Process ◽  
Vibration Band ◽  
Ftir Analysis ◽  
Tuna Fish ◽  
Bioactive Component ◽  
Cosmetic Industry ◽  
Nanofiber Diameter

Catechin and gelatin are important natural products for food, medical, pharmaceutical and cosmetic industry. We have successfully synthesized catechin-gelatin nanofiber by electrospinning process. Catechin-gelatin nanofiber was synthesized by using gelatin from yellow fin skin tuna fish as biopolymer, polyethylene oxide (PEO) as spinnability improver polymer, acetic acid as solvent and catechin as bioactive component, respectively. Morphology and structure of bioactive catechin-gelatin nanofiber were characterized by scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR), respectively. SEM analysis showed that morphology of nanofiber was very smooth without bead on nanofiber string. The average of catechin-gelatin nanofiber diameter was 389 nm. FTIR analysis results were used to confirm structure of catechin-gelatin nanofiber. Catechin-gelatin nanofiber has vibration band peak of amide A (N-H) at 3289,043 cm-1 and amide B (N-H) 3062,310 cm-1, amide I (C=O) at 1643,812 cm-1, amide II (N-H and CN) at 1538,949 cm-1, amide III (C-N) at 1237,11 cm-1 from gelatin, C-O-C from PEO at 1143,583 cm-1, and vibration band peak OH at 3200-3600 cm-1, and at C-O ether around 1300-1100 from catechin, respectively. FTIR spectra showed us that there is no change in chemical structure of gelatin and catechin in nanofiber which was produced by electrospinning process. Catechin-gelatin nanofiber can inhibit S. Aureus bacteria around 43.38%

Dual-Charge Nanofiber Mats Made of Chitosan(CS)/Poly(Vinyl Alcohol) (PVA) and Poly-(Acrylic Acid-Co-Maleic Acid) (PAMA)/PVA

2019 ◽  
Vol 819 ◽  
pp. 145-150
Author(s):  
Thapakorn Chareonying ◽  
Junnasir M. Sakilan ◽  
Theerasak Rojanarata ◽  
Prasopchai Patrojanasophon ◽  
Prasert Akkaramongkolporn ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Maleic Acid ◽  
Vinyl Alcohol ◽  
Electrospinning Process ◽  
Poly Vinyl Alcohol ◽  
Water Capacity ◽  
Ft Ir ◽  
Nanofiber Mats ◽  
Positively Charged ◽  
Poly Acrylic Acid

Nanofibers have been widely used for tissue engineering. Using charged polymers for the preparation of nanofibers can be useful for the loading of substances or macromolecules. Dual charge nanofiber mats are expected to be able to immobilize both positively charged and negatively charged substances in one versatile nanofiber mat. The purpose of this study was to prepare and characterize dual-charge nanofibers generated from poly (vinyl alcohol) (PVA)/poly-(acrylic acid-co-maleic acid) (PAMA) and chitosan (CS)/PVA. The polymer solutions of PAMA/PVA (1:1.63 w/w) and CS/PVA (1:2.33 w/w) were electrospun to form the nanofibers using dual-jet electrospinning process. The obtained dual-charge nanofibers were thermally crosslinked by leaving the nanofibers in the oven at 110-130 °C for 0.5, 1, 3, 5 h. The appearance of the nanofiber mat was characterized by a scanning electron microscope (SEM), and the diameter of nanofibers were determined by an image analysis software (J-micro vision®). The percentage water insolubilization and FT-IR spectra were also determined. The dual-size nanofiber mats with smooth and bead-free fibers were obtained. The diameter of the PAMA/PVA and CS/PVA fibers was 574.54 ± 142.98 nm and 225.69 ± 41.92 nm, respectively. The desirable temperature and time for the crosslink of the dual-charge nanofiber mats was 130 °C for 1 h which could provide a high insolubilization with water capacity of 93.22 ± 2.23%.

Optimization and physical performance evaluation of electrospun nanofibrous mats of PLA, PCL and their blends

2020 ◽  
pp. 152808372094450
Author(s):  
Deepika Sharma ◽  
Bhabani K Satapathy
Keyword(s):  
Physical Performance ◽  
Process Parameters ◽  
Optimization Technique ◽  
Electrospinning Process ◽  
Sequential Optimization ◽  
Orifice Diameter ◽  
Solvent Ratio ◽  
Solvent Mixtures ◽  
Minimum Diameter ◽  
Hierarchical Processing

The optimization of process parameters such as applied voltage, orifice diameter, solvent system, and solvent ratio for electrospinning of neat polymers, polylactic acid (PLA) and poly (є-caprolactone) (PCL), to obtain uniform, randomly oriented nanofibers with minimum diameter variation and beaded structures has been critically discussed. The paper focuses on establishing a sequential optimization technique for arriving at a common set of electrospinning process parameters for individual polymers, such as, applied voltages, orifice diameters, solvent mixtures, solvent ratios, to be used in the fabrication of electrospun nanofibrous mats (ENMs) of blended polymers. In this study, the effect of variation of applied voltages, orifice diameters, solvent mixtures, solvent ratios, PLA/PCL blending ratios, solution concentration of blends and flow rate were reported via morphological analysis of electrospun nanofibers. The set of optimal process parameters obtained for both PLA and PCL were adopted for the fabrication of ENMs based on the PLA/PCL blends. The paper further deliberates on the physical performance of PLA/PCL based ENMs in acidic, basic and neutral release media. Thus, the study establishes a hierarchical processing optimization route for designing blended ENMs by following a set of variable electrospinning process parameters.

scholarly journals Engineering the hard–soft tissue interface with random-to-aligned nanofiber scaffolds

2018 ◽  
Vol 5 ◽  
pp. 184954351880353 ◽  
Author(s):  
John Nowlin ◽  
Mehzubh A Bismi ◽  
Baptiste Delpech ◽  
Patrick Dumas ◽  
Yingge Zhou ◽  
...  
Keyword(s):  
Surgical Repair ◽  
Polymer Concentration ◽  
Electrospinning Process ◽  
Gradual Transition ◽  
Fibroblast Cells ◽  
Stress Concentrations ◽  
Bone Interface ◽  
Nanofiber Scaffold ◽  
Tissue Interface ◽  
Cell Phenotypes

Tendon injuries can be difficult to heal and have high rates of relapse due to stress concentrations caused by scar formation and the sutures used in surgical repair. Regeneration of the tendon/ligament-to-bone interface is critical to provide functional graft integration after injury. The objective of this study is to recreate the tendon-to-bone interface using a gradient scaffold which is fabricated by a one-station electrospinning process. Two cell phenotypes were grown on a poly- ε-caprolactone nanofiber scaffold which possesses a gradual transition from random to aligned nanofiber patterns. We assessed the effects of the polymer concentration, tip-to-collector distance, and electrospinning time on the microfiber diameter and density. Osteosarcoma and fibroblast cells were seeded on the random and aligned sections of scaffolds, respectively. A random-to-aligned cocultured tissue interface which mimicked the native transition in composition of enthesis was created after 96 h culturing. The results showed that the microstructure gradient influenced the cell morphology, tissue topology, and promoted enthesis formation. This study demonstrates a heterogeneous nanofiber scaffold strategy for interfacial tissue regeneration. It provides a potential solution for mimicking transitional interface between distinct tissues, and can be further developed as a heterogeneous cellular composition platform to facilitate the formation of multi-tissue complex systems.

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