scholarly journals Nano Iron Oxide-PCL Composite as an Improved Soft Tissue Scaffold

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1559
Author(s):  
Vahid Rezaei ◽  
Esmaeil Mirzaei ◽  
Seyedeh-Masoumeh Taghizadeh ◽  
Aydin Berenjian ◽  
Alireza Ebrahiminezhad
Keyword(s):  
Iron Oxide ◽  
Soft Tissue ◽  
Fe3o4 Nanoparticles ◽  
Cell Attachment ◽  
Growth Promotion ◽  
Precipitation Reaction ◽  
Promoting Effect ◽  
Electrospinning Technique ◽  
Tissue Scaffold ◽  
Nanocomposite Scaffolds

Iron oxide nanoparticles were employed to fabricate a soft tissue scaffold with enhanced physicochemical and biological characteristics. Growth promotion effect of L-lysine coated magnetite (Lys@Fe3O4) nanoparticles on the liver cell lines was proved previously. So, in the current experiment these nanoparticles were employed to fabricate a soft tissue scaffold with growth promoting effect on the liver cells. Lys@Fe3O4 nanoparticles were synthesized via co-precipitation reaction. Resulted particles were ~7 nm in diameter and various concentrations (3, 5, and 10 wt%) of these nanoparticles were used to fabricate nanocomposite PCL fibers. Electrospinning technique was employed and physicochemical characteristics of the resulted nanofibers were evaluated. Electron micrographs and EDX-mapping analysis showed that nanoparticles were well dispersed in the PCL fibers and no bead structure were formed. As expected, incorporation of Lys@Fe3O4 to the PCL nanofibers resulted in a reduction in hydrophobicity of the scaffold. Nanocomposite scaffolds were shown increased tensile strength with increasing concentration of employed nanoparticles. In contrast to PCL scaffold, nearly 150% increase in the cell viability was observed after 3-days exposure to the nanocomposite scaffolds. This study indicates that incorporation of magnetite nanoparticles in the PCL fibers make them more prone to cell attachment. However, incorporated nanoparticles can provide the attached cells with valuable iron element and consequently promote the cells growth rate. Based on the results, magnetite enriched PCL nanofibers could be introduced as a scaffold to enhance the biological performance for liver tissue engineering purposes.

scholarly journals Development of 3D Bioactive Nanocomposite Scaffolds Made from Gelatin and Nano Bioactive Glass for Biomedical Applications

2010 ◽  
Vol 19 (2) ◽  
pp. 096369351001900 ◽  
Author(s):  
M. Mozafari ◽  
F. Moztarzadeh ◽  
M. Rabiee ◽  
M. Azami ◽  
N. Nezafati ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Biomedical Applications ◽  
Cell Attachment ◽  
Tissue Engineering Scaffolds ◽  
Study Results ◽  
Nanocomposite Scaffold ◽  
Nanocomposite Scaffolds ◽  
First Time

In this research, macroporous, mechanically competent and bioactive nanocomposite scaffolds have been fabricated from cross-linked gelatine (Gel) and nano bioactive glass (nBG) through layer solvent casting combined with freeze-drying and lamination techniques. This study has developed a new composition to produce a new bioactive nanocomposite which is porous with interconnected microstructure, pore sizes are 200-500 μm, porosity are 72%-86%. Also, we have reported formation of chemical bonds between nBG and Gel for the first time. Finally, the in vitro cytocompatability of the scaffolds was assessed using MTT assay and cell attachment study. Results indicated no sign of toxicity and cells found to be attached to the pore walls offered by the scaffolds. These results suggested that the developed nanocomposite scaffold possess the prerequisites for bone tissue engineering scaffolds and it can be used for tissue engineering applications.

Use of Bioabsorbable Tissue Reinforcement Reduces Incidence of Internal Hernia in Roux-en-Y Gastric Bypass Patients

2018 ◽  
Vol 84 (11) ◽  
pp. 1756-1761
Author(s):  
Michael W. Love ◽  
Roosbeh Mansour ◽  
Allyson L. Hale ◽  
Eric S. Bour ◽  
Ihab Shenouda ◽  
...  
Keyword(s):  
Gastric Bypass ◽  
Soft Tissue ◽  
Internal Hernia ◽  
Tissue Scaffold ◽  
Internal Hernias ◽  
Early Evidence ◽  
Hernia Formation ◽  
Tissue Matrix ◽  
Tissue Defects ◽  
Prospective Database

Internal hernias are one of the most devastating late, postsurgical complications associated with laparoscopic Roux-en-Y gastric bypass (LRYGB). The objective of this study was to determine whether placement of a bioabsorbable tissue matrix in soft tissue defects after gastric bypass resulted in a lower incidence of internal hernia development. Prospective database was used to identify all patients who underwent LRYGB between January 2002 and January 2016. These patients were then retrospectively reviewed to determine the development of internal hernia. Before 2009, the retro-Roux defect was left open during the primary operation and the defect at the jejunojejunostomy was closed with sutures or staples. Beginning in 2009, all soft tissue internal defects were reinforced with an 8 cm 3 8-cm piece of bioabsorbable matrix. The incidence of subsequent internal hernia development was compared between these two groups: no bioabsorbable matrix versus use of a bioabsorbable matrix. A total of 2771 patients underwent LRYGB during our study period. From these, 1215 procedures were performed without tissue reinforcement and 1556 were performed using a bioabsorbable matrix. During the study period, 274 patients developed an internal hernia. Patients who did not have tissue reinforcement at closure had a significantly higher internal hernia rate [225/1215 (18.5%) vs 49/1556 (3.1%), P < 0.005]. This study demonstrates a statistically significant reduction in internal hernia formation after LRYGB with the addition of a bioabsorbable tissue matrix. Although prospective studies are needed, early evidence suggests that reinforcement with a bioabsorbable tissue scaffold is an effective method for minimizing internal hernias after LRYGB.

scholarly journals The biological seal of the implant–soft tissue interface evaluated in a tissue-engineered oral mucosal model

2012 ◽  
Vol 9 (77) ◽  
pp. 3528-3538 ◽  
Author(s):  
Wen L. Chai ◽  
Ian M. Brook ◽  
Anders Palmquist ◽  
Richard van Noort ◽  
Keyvan Moharamzadeh
Keyword(s):  
Soft Tissue ◽  
Cell Attachment ◽  
Tritiated Water ◽  
Three Dimensional ◽  
Permeability Test ◽  
Significant Difference ◽  
Surface Topographies ◽  
Tissue Interface

For dental implants, it is vital that an initial soft tissue seal is achieved as this helps to stabilize and preserve the peri-implant tissues during the restorative stages following placement. The study of the implant–soft tissue interface is usually undertaken in animal models. We have developed an in vitro three-dimensional tissue-engineered oral mucosal model (3D OMM), which lends itself to the study of the implant–soft tissue interface as it has been shown that cells from the three-dimensional OMM attach onto titanium (Ti) surfaces forming a biological seal (BS). This study compares the quality of the BS achieved using the three-dimensional OMM for four types of Ti surfaces: polished, machined, sandblasted and anodized (TiUnite). The BS was evaluated quantitatively by permeability and cell attachment tests. Tritiated water (HTO) was used as the tracing agent for the permeability test. At the end of the permeability test, the Ti discs were removed from the three-dimensional OMM and an Alamar Blue assay was used for the measurement of residual cells attached to the Ti discs. The penetration of the HTO through the BS for the four types of Ti surfaces was not significantly different, and there was no significant difference in the viability of residual cells that attached to the Ti surfaces. The BS of the tissue-engineered oral mucosa around the four types of Ti surface topographies was not significantly different.

Size-dependent magnetic and inductive heating properties of Fe3O4 nanoparticles: scaling laws across the superparamagnetic size

2018 ◽  
Vol 20 (18) ◽  
pp. 12879-12887 ◽  
Author(s):  
Jeotikanta Mohapatra ◽  
Fanhao Zeng ◽  
Kevin Elkins ◽  
Meiying Xing ◽  
Madhav Ghimire ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Fe3o4 Nanoparticles ◽  
Absorption Rate ◽  
Scaling Laws ◽  
Specific Absorption Rate ◽  
Nanoparticle Size ◽  
Inductive Heating ◽  
Specific Absorption ◽  
Size Dependent

An efficient heat activating mediator with an enhanced specific absorption rate (SAR) value is attained via control of the iron oxide (Fe3O4) nanoparticle size from 3 to 32 nm.

scholarly journals Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein

Medicina ◽  
2019 ◽  
Vol 55 (10) ◽  
pp. 682 ◽  
Author(s):  
Sena ◽  
Sumeyra ◽  
Ulkugul ◽  
Sema ◽  
Betul ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Metformin Hydrochloride ◽  
Core Shell ◽  
Order Kinetic ◽  
Drug Release Profile ◽  
Scanning Calorimetry ◽  
Electrospinning Technique ◽  
Sarcoplasmic Protein ◽  
Enhance Cell Attachment ◽  
Diabetic Wound Healing

Background and Objectives: A coaxial electrospinning technique was used to produce core/shell nanofibers of a polylactic acid (PLA) as a shell and a polyvinyl alcohol (PVA) containing metformin hydrochloride (MH) as a core. Materials and Methods: Fish sarcoplasmic protein (FSP) was extracted from fresh bonito and incorporated into nanofiber at various concentrations to investigate the influence on properties of the coaxial nanofibers. The morphology, chemical structure and thermal properties of the nanofibers were studied. Results: The results show that uniform and bead-free structured nanofibers with diameters ranging from 621 nm to 681 nm were obtained. A differential scanning calorimetry (DSC) analysis shows that FSP had a reducing effect on the crystallinity of the nanofibers. Furthermore, the drug release profile of electrospun fibers was analyzed using the spectrophotometric method. Conclusions: The nanofibers showed prolonged and sustained release and the first order kinetic seems to be more suitable to describe the release. MTT assay suggests that the produced drug and protein loaded coaxial nanofibers are non-toxic and enhance cell attachment. Thus, these results demonstrate that the produced nanofibers had the potential to be used for diabetic wound healing applications.

THE BIOASSAY OF GROWTH HORMONE IN SNELL'S DWARF MICE: EFFECTS OF THYROXINE AND PROLACTIN ON THE DOSE—RESPONSE CURVE

1973 ◽  
Vol 56 (2) ◽  
pp. 235-243 ◽  
Author(s):  
M. WALLIS ◽  
JENNIFER A. DEW
Keyword(s):  
Growth Hormone ◽  
Response Curve ◽  
Growth Response ◽  
Growth Promotion ◽  
Promoting Effect ◽  
Pituitary Growth Hormone ◽  
Growth Promoting ◽  
Dependent Growth ◽  
Dwarf Mice ◽  
Dose Dependent

SUMMARY Pituitary growth hormone has a dose-dependent growth promoting effect in pituitary dwarf mice (Snell's strain), and this effect can be used as the basis of a bioassay for the hormone. Prolactin and thyroxine also promote growth in these animals, and the effects of these hormones in combination with growth hormone were studied, in order to see whether their presence might enhance the precision or sensitivity of the growth hormone assay. When prolactin and/or thyroxine were administered with growth hormone, the growth response observed was no greater than the sum of the effects of the hormones given separately; in some cases it was less. Neither prolactin nor thyroxine increase the sensitivity or precision of the growth hormone bioassay. The implications of these results for theories about the mechanisms of growth promotion by these hormones are considered.

Synthesis and Aqueous Dispersion of Monodisperse Magnetic Fe3O4 Nanoparticles

2013 ◽  
Vol 668 ◽  
pp. 335-337
Author(s):  
Wan Ju Zhang ◽  
Fang Wang
Keyword(s):  
Thermal Decomposition ◽  
Iron Oxide ◽  
Low Temperature ◽  
Fe3o4 Nanoparticles ◽  
Large Scale ◽  
Low Cost ◽  
Aqueous Dispersion ◽  
Amphiphilic Copolymer ◽  
Magnetic Fe3o4 Nanoparticles ◽  
Magnetic Fe3o4

Iron oxide (Fe3O4) nanoparticles as one of the most important nanomaterials are suitable for many applications. Monodisperse magnetic Fe3O4 nanoparticles were synthesized by the thermal decomposition of the iron oleate precursor in octadecene (ODE). The iron oleate complex was prepared by reaction between sodium oleic and FeCl3·6H2O at low temperature. The Fe3O4 nanoparticles were transferred from organic solvent into water by amphiphilic copolymer. The aqueous dispersion of Fe3O4 nanoparticles was stable in water and physiological buffers. This method with low cost can be used to prepare large scale of aqueous dispersion of Fe3O4 nanoparticles.

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