Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations

Assessment of the diversity of reproductive traits and genetic variation is of great relevance to the conservation of genetic resources and management of silver fir (Abies alba) populations. We have evaluated reproductive characteristics associated with female cones and seed morphology, as well as seed germination after subjecting seeds to five storage methods, in nine Romanian populations of A. alba. The genetic diversity of the populations was assessed with 12 polymorphic simple sequence repeat (SSR) markers. We detected significant differences between populations for all reproductive traits and considerable differences in seed germination and storage methods; seed storage in wet sand was the method resulting in the highest germination in all populations. Genomic SSRs (gSSRs) were more informative on average than expressed sequence tag SSRs (EST-SSRs) in the populations studied. The nine populations were genetically diverse, with an average number of alleles (N) per SSR locus between 3.50 and 4.83. The observed heterozygosity (Ho) in the nine populations was always lower than the expected heterozygosity (He), which resulted in values of the inbreeding coefficient (Fis) between 0.261 and 0.709. Genetic distances between populations ranged between 0.077 and 0.410. The cluster analysis based on genetic distances did not group accessions according to their geographical proximity, and despite a positive trend, the correlation between geographic and genetic distances was non-significant. The results of an analysis of molecular variance (AMOVA) revealed that only 9.1% of the total molecular variance is attributable to differences between populations. This low degree of genetic differentiation between populations is confirmed by the intermingling of individuals of different populations in a principal coordinate analysis (PCoA). We found evidence of a positive relationship between He and germination, as well as a negative one between Fis and germination, suggesting that populations with low diversity and high consanguinity may have a reduced fitness and long-term viability. The results are relevant for the conservation and management of local genetic resources and populations, as well as for reforestation programmes of silver fir.

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Genetic analysis of silver-fir populations in the Beskids

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2003.015 ◽

2011 ◽

Vol 72 (2) ◽

pp. 115-119 ◽

Cited By ~ 3

Author(s):

Leon Mejnartowicz

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Genetic Analysis ◽

Abies Alba ◽

Genetic Distances ◽

Silver Fir ◽

Geographical Distance ◽

Polymorphic Loci ◽

Expected Heterozygosity ◽

Rare Alleles

Twenty-eight isozymic loci were studied in the Beskid Mts., in four populations of common silver-fir (Abies alba): one in Beskid Makowski (BM) and three populations in Beskid Sądecki (BS). Their genetic variation and diversity were analyzed, and Nei's genetic distances between the populations were calculated. The results show that the geographical distance between the BM population and the three BS populations is reflected in genetic distances. The BM population is clearly distinct from the others. It has the lowest genetic diversity (I = 0.42), percentage of polymorphic loci (%PoL = 64.29) and number of rare alleles (NoRa = 5). Besides, the BM population has the highest observed heterozygosity (Ho = 0.291), which exceeds the expected heterozygosity (He = 0.254), estimated on the basis of the Hardy-Weinberg Principle. On the contrary, BS populations are in the state of equilibrium, which is manifested, in similar values of He = 0.262 and Ho = 0.264.

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467 PB 330 RAPD ASSAY TO IMPROVE BRASSICA GENETIC RESOURCES COLLECTION MANAGEMENT

HortScience ◽

10.21273/hortsci.29.5.498b ◽

1994 ◽

Vol 29 (5) ◽

pp. 498b-498

Author(s):

Winthrop B. Phippen ◽

James R. McFerson ◽

Stephen Kresovich

Keyword(s):

Genetic Resources ◽

Rapd Markers ◽

Genetic Relationships ◽

Molecular Genetic ◽

Cost Effective ◽

Genetic Distances ◽

Gene Bank ◽

Collection Management ◽

Specific Level ◽

Bank Management

Genetic variation and relationships in genetic resources collections can be assessed using molecular genetic markers. We examined the applicability of the RAPD assay for quick, cost-effective, and reliable use in improving collection management. Fourteen accessions of Brassica oleracea spp. capitata `Golden Acre' (cabbage) were screened using nine decamer oligonucleotide primers. We obtained 110 reproducible fragments, of which 80 were polymorphic, ranging in size from 370-1730 bp. Individual accessions were readily distinguished. A cluster analysis of genetic distances generated by bootstrapping reflected all known genetic relationships, except one. Bulking strategies were also investigated. RAPD markers can be applied to gene bank management to measure variation, identify accessions, and establish genetic similarity at the intra-specific level addressing the needs of both curators and users.

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Genetic diversity and population structure of Brazilian native bovine breeds

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2004000600005 ◽

2004 ◽

Vol 39 (6) ◽

pp. 543-549 ◽

Cited By ~ 16

Author(s):

Germana Maria Serrano ◽

Andréa Alves do Egito ◽

Concepta McManus ◽

Arthur da Silva Mariante

Keyword(s):

Bos Taurus ◽

Genetic Relationships ◽

Genetic Distances ◽

The Other ◽

Phenotypic Characteristics ◽

Analysis Of Molecular Variance ◽

Molecular Variance ◽

Improvement Strategies ◽

Polymorphic Bands ◽

The Individual

Conservation and improvement strategies should be based on the association between genetic and phenotypic characteristics. The objective of this work was to characterize five native Brazilian cattle breeds (Caracu, Crioulo Lageano, Curraleiro, National Polled and Pantaneiro) and two commercial breeds (Holstein and Nellore) using RAPD technique to estimate genetic distances and variability between and within breeds. Genetic relationships were investigated using 22 primers which generated 122 polymorphic bands. Analysis of molecular variance indicated that most of the genetic variation lay among individuals within populations. The genetic variabilities between pairs of breeds were statistically significant. The smallest genetic divergence was between Crioulo Lageano and Curraleiro.The National Polled, although historically considered to be of Bos taurus aquitanicus origin,similar to theCaracu, was grouped together with the other breeds of Bos taurus ibericus origin. Generally, the individual breeds formed distinct clusters except the National Polled. The RAPD technique was capable to distinguish genetically between the breeds studied; the Caracu, Crioulo Lageano, Curraleiro and Pantaneiro may be considered distinct genetic entities thereby proving the uniqueness of the populations; the National Polled has not been able to re-establish itself after its decline in the 1950s, thereby losing its genetic identity.

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Genetic analysis of silver-fir populations in the North Carpathian and Sudeten Mountains

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2004.036 ◽

2011 ◽

Vol 73 (4) ◽

pp. 285-292 ◽

Cited By ~ 11

Author(s):

Leon Mejnartowicz

Keyword(s):

Gene Flow ◽

Abies Alba ◽

Genetic Distances ◽

Silver Fir ◽

Starch Gel Electrophoresis ◽

Effective Number ◽

The North ◽

Rare Alleles ◽

The Mean ◽

Individual Trees

Seeds collected from individual trees in the 16 Carpathian and 2 Sudeten silver-fir (Abies alba) populations were studied with the starch gel electrophoresis in megagametophytes using 14 enzyme systems with 28 loci. The results show that the geographical distance between populations are in a small part reflected in genetic distances. There are two main groups of populations: Sudeten and Carpathian with a very big genetic distance between them. Other populations consist of a few small groups with low gene flow between them (Nm = 3.286). About 80% of genetic variation is located within populations (FST = 0.223). Average values for genetic multiplicity and diversity for Carpathian populations are as follows: number of alleles per locus: Na = 2.308, with effective number of alleles Ne = 1.552 and proportion of polymorphic loci 71.21%. The mean number of alleles per locus (Na) varied from 2.107 to 2.607 in population. The mean effective number of alleles per locus (Ne) ranged from 1.429 to 1.662. Average Fis for Carpathian populations was -0.021, which means that there is small excess of heterozygotes. The average observed heterozygosity amounted to Ho = 0.275 and expected heterozygosity was He = 0.269. The dendrogram structure and presence of rare alleles found in silver-fir of Czech, and Slovakian populations allow for a hypothesis that in postglaciation the silver-fir moved into the Polish Carpathians not westward from the east but from the south along river valleys from some Balkan refuges, getting North bypassing the High Tatra Range. This way, a highly diversified set of populations originated, differ in the presence of rare alleles. This differentiation is not prevented by a relatively small flow of genes between populations. The calculated gene flow Nm = 3.286 also indicates isolation between the populations. It means 3.3 immigrants per generation into the studied populations.

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Effect of earthworms on mycorrhization, root morphology and biomass of silver fir seedlings inoculated with black summer truffle (Tuber aestivum Vittad.)

Scientific Reports ◽

10.1038/s41598-021-85497-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Tina Unuk Nahberger ◽

Gian Maria Niccolò Benucci ◽

Hojka Kraigher ◽

Tine Grebenc

Keyword(s):

Root System ◽

Root Biomass ◽

Fine Root ◽

Abies Alba ◽

Fine Root Biomass ◽

Root Tip ◽

Silver Fir ◽

Tuber Aestivum ◽

Root Tips ◽

Root Parameters

AbstractSpecies of the genus Tuber have gained a lot of attention in recent decades due to their aromatic hypogenous fruitbodies, which can bring high prices on the market. The tendency in truffle production is to infect oak, hazel, beech, etc. in greenhouse conditions. We aimed to show whether silver fir (Abies alba Mill.) can be an appropriate host partner for commercial mycorrhization with truffles, and how earthworms in the inoculation substrate would affect the mycorrhization dynamics. Silver fir seedlings inoculated with Tuber. aestivum were analyzed for root system parameters and mycorrhization, how earthworms affect the bare root system, and if mycorrhization parameters change when earthworms are added to the inoculation substrate. Seedlings were analyzed 6 and 12 months after spore inoculation. Mycorrhization with or without earthworms revealed contrasting effects on fine root biomass and morphology of silver fir seedlings. Only a few of the assessed fine root parameters showed statistically significant response, namely higher fine root biomass and fine root tip density in inoculated seedlings without earthworms 6 months after inoculation, lower fine root tip density when earthworms were added, the specific root tip density increased in inoculated seedlings without earthworms 12 months after inoculation, and general negative effect of earthworm on branching density. Silver fir was confirmed as a suitable host partner for commercial mycorrhization with truffles, with 6% and 35% mycorrhization 6 months after inoculation and between 36% and 55% mycorrhization 12 months after inoculation. The effect of earthworms on mycorrhization of silver fir with Tuber aestivum was positive only after 6 months of mycorrhization, while this effect disappeared and turned insignificantly negative after 12 months due to the secondary effect of grazing on ectomycorrhizal root tips.

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Impact of Industrial Pollution on Radial Growth of Conifers in a Former Mining Area in the Eastern Carpathians (Northern Romania)

Forests ◽

10.3390/f12050640 ◽

2021 ◽

Vol 12 (5) ◽

pp. 640

Author(s):

Cristian Gheorghe Sidor ◽

Radu Vlad ◽

Ionel Popa ◽

Anca Semeniuc ◽

Ecaterina Apostol ◽

...

Keyword(s):

Air Pollution ◽

Industrial Pollution ◽

Radial Growth ◽

Abies Alba ◽

Mining Operation ◽

Mining Area ◽

Silver Fir ◽

Local Pollution ◽

Eastern Carpathians ◽

The Impact

The research aims to evaluate the impact of local industrial pollution on radial growth in affected Norway spruce (Picea abies (L.) Karst.) and silver fir (Abies alba Mill.) stands in the Tarnița study area in Suceava. For northeastern Romania, the Tarnița mining operation constituted a hotspot of industrial pollution. The primary processing of non-ferrous ores containing heavy metals in the form of complex sulfides was the main cause of pollution in the Tarnița region from 1968 to 1990. Air pollution of Tarnița induced substantial tree growth reduction from 1978 to 1990, causing a decline in tree health and vitality. Growth decline in stands located over 6 km from the pollution source was weaker or absent. Spruce trees were much less affected by the phenomenon of local pollution than fir trees. We analyzed the dynamics of resilience indices and average radial growth indices and found that the period in which the trees suffered the most from local pollution was between 1978 and 1984. Growth recovery of the intensively polluted stand was observed after the 1990s when the environmental condition improved because of a significant reduction in air pollution.

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Reduced climate sensitivity of carbon, oxygen and hydrogen stable isotope ratios in tree-ring cellulose of silver fir (Abies alba Mill.) influenced by background SO2 in Franconia (Germany, Central Europe)

Environmental Pollution ◽

10.1016/j.envpol.2013.10.030 ◽

2014 ◽

Vol 185 ◽

pp. 281-294 ◽

Cited By ~ 16

Author(s):

Tatjana Boettger ◽

Marika Haupt ◽

Michael Friedrich ◽

John S. Waterhouse

Keyword(s):

Stable Isotope ◽

Central Europe ◽

Tree Ring ◽

Climate Sensitivity ◽

Abies Alba ◽

Isotope Ratios ◽

Stable Isotope Ratios ◽

Silver Fir ◽

Tree Ring Cellulose

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Genetic diversity in table grapes based on RAPD and microsatellite markers

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2011000900010 ◽

2011 ◽

Vol 46 (9) ◽

pp. 1035-1044 ◽

Cited By ~ 9

Author(s):

Patrícia Coelho de Souza Leão ◽

Sérgio Yoshimitsu Motoike

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Genetic Relationships ◽

Similarity Index ◽

Genetic Distances ◽

Table Grape ◽

Group Method ◽

Molecular Characteristics ◽

Table Grapes ◽

Pair Group

The objective of this work was to analyze the genetic diversity of 47 table grape accessions, from the grapevine germplasm bank of Embrapa Semiárido, using 20 RAPD and seven microsatellite markers. Genetic distances between pairs of accessions were obtained based on Jaccard's similarity index for RAPD data and on the arithmetic complement of the weighted index for microsatellite data. The groups were formed according to the Tocher's cluster analysis and to the unweighted pair‑group method with arithmetic mean (UPGMA). The microsatellite markers were more efficient than the RAPD ones in the identification of genetic relationships. Information on the genetic distance, based on molecular characteristics and coupled with the cultivar agronomic performance, allowed for the recommendation of parents for crossings, in order to obtain superior hybrids in segregating populations for the table grape breeding program of Embrapa Semiárido.

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