scholarly journals Cylindrospermopsin- and Deoxycylindrospermopsin-Producing Raphidiopsis raciborskii and Microcystin-Producing Microcystis spp. in Meiktila Lake, Myanmar

Toxins ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 232 ◽  
Author(s):  
Andreas Ballot ◽  
Thida Swe ◽  
Marit Mjelde ◽  
Leonardo Cerasino ◽  
Vladyslava Hostyeva ◽  
...  
Keyword(s):  
Toxin Production ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Biological Parameters ◽  
Liquid Chromatography Mass Spectrometry ◽  
Phytoplankton Composition ◽  
Fresh Weight ◽  
Physico Chemical ◽  
Biomass Determination ◽  
Harmful Effects

Meiktila Lake is a shallow reservoir located close to Meiktila city in central Myanmar. Its water is used for irrigation, domestic purposes and drinking water. No detailed study of the presence of cyanobacteria and their potential toxin production has been conducted so far. To ascertain the cyanobacterial composition and presence of cyanobacterial toxins in Meiktila Lake, water samples were collected in March and November 2017 and investigated for physico-chemical and biological parameters. Phytoplankton composition and biomass determination revealed that most of the samples were dominated by the cyanobacterium Raphidiopsis raciborskii. In a polyphasic approach, seven isolated cyanobacterial strains were classified morphologically and phylogenetically as R. raciborskii, and Microcystis spp. and tested for microcystins (MCs), cylindrospermopsins (CYNs), saxitoxins and anatoxins by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography–mass spectrometry (LC–MS). ELISA and LC–MS analyses confirmed CYNs in three of the five Raphidiopsis strains between 1.8 and 9.8 μg mg−1 fresh weight. Both Microcystis strains produced MCs, one strain 52 congeners and the other strain 20 congeners, including 22 previously unreported variants. Due to the presence of CYN- and MC-producing cyanobacteria, harmful effects on humans, domestic and wild animals cannot be excluded in Meiktila Lake.

Effect of Preservation on the Weight of Marine Benthic Invertebrates

1982 ◽  
Vol 39 (1) ◽  
pp. 221-224 ◽  
Author(s):  
Eric L. Mills ◽  
Karin Pittman ◽  
Brent Munroe
Keyword(s):  
Benthic Invertebrates ◽  
Dry Weight ◽  
The Other ◽  
Tissue Composition ◽  
Fresh Weight ◽  
Short Term ◽  
Long Term Changes ◽  
Biomass Determination ◽  
Marine Benthic Invertebrates

It would be convenient if preserved animals could be used to determine fresh-weight biomass. However, marine annelids, nematodes, bivalves, amphipods, and isopods all lost weight when preserved and stored in 70% isopropanol. In 10% formalin only bivalves lost weight significantly; the other groups showed evidence of small increases or decreases. Many animals preserved in alcohol decreased sharply in weight within a few minutes, probably due to dehydration. In formalin most increased at first, then slowly decreased. The response to preservatives is complex, involving short-term changes of water content and long-term changes of tissue composition. Precision, though not accuracy, of preserved wet weights can only be achieved after specimens have been in preservatives for a month or more, especially in the case of isopropanol. For these reasons, wet and dry weight biomass figures should be determined from fresh, unpreserved animals.Key words: fixation, preservation (organisms), biomass determination, benthos

scholarly journals Physico-chemical characteristics of pomegranate accessions from the Kurdistan region, Iraq

2018 ◽  
Vol 63 (4) ◽  
pp. 355-366
Author(s):  
Saadatian Mohammad ◽  
Paiza Abdurahman ◽  
Kanar Salim ◽  
Pershang Younis ◽  
Hewen Abdurahman ◽  
...  
Keyword(s):  
Fruit Size ◽  
Chemical Properties ◽  
Fruit Weight ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Kurdistan Region ◽  
Fresh Weight ◽  
Fruit Length ◽  
Physico Chemical ◽  
Fruit Diameter

Some physico-chemical properties of ten pomegranate accessions collected from different districts in the Kurdistan region of Iraq were investigated in this paper. Considerable correlations between the characteristics studied were found and valuable pomological traits were observed. Cluster analysis showed the homonyms between some pomegranate accessions. Principle component analysis reported that the component describing the greatest variability also positively correlated with fruit weight, total aril weight, total peel weight, volume of juice, total soluble solids (TSS), fruit length, fruit diameter, pH, aril length, and 100-seed fresh weight, but negatively correlated with titratable acidity (TA). Fruit weight was firmly correlated with total aril weight, total peel weight, volume of juice, TSS, aril length, 100-seed fresh weight, fruit length and fruit diameter. The volume of juice was correlated with TSS, aril length, 100-seed fresh weight, fruit length, fruit diameter and it was observed that with an increase in the fruit size, the volume of juice increased as well. The correlation between total phenolic compounds and antioxidant capacity was not observed. The associations found among physical and chemical traits suggest that consumers should use large fruits with large arils so that they have more juice. Thus, ?Choman?, ?Raniyeh? and ?Halabja? were juicier than other accessions.

scholarly journals Effect of Cassia occidentalis on Parthenium hysterophorus by way of secondary metabolites

2016 ◽  
Vol 5 (11) ◽  
pp. 1451
Author(s):  
Jai Knox
Keyword(s):  
Dry Weight ◽  
Suppressive Effect ◽  
Significant Inhibition ◽  
Parthenium Hysterophorus ◽  
Biological Parameters ◽  
Fresh Weight ◽  
Cassia Occidentalis ◽  
Medicinal Value ◽  
Biochemical Interaction ◽  
Relative Water

Cassia occidentalis is an extensive growing weed in India with some medicinal value. It has been reported to show strong biochemical interaction causing inhibition of Parthenium hysterophorus which is an obnoxious weed of today. The present study was performed to confirm its inhibition on Parthenium. Biological parameters like Seed germination, Germination velocity index, Shoot length and Root length of 10 days old Parthenium seedlings was recorded. Fresh weight (FW), Dry weight (DW) and Relative water content% (RWC) was recorded after treating the mature Parthenium plant with various aqueous leaf leachates of Cassia occidentalis. Aqueous leaf leachates of Cassia occidentalis which were prepared in different concentrations showed significant inhibition in all biological parameters on Parthenium. This study gives a strong clue regarding the potential of suppressive effect of allelochemicals present in Cassia on Parthenium.

scholarly journals Physico-chemical and Sensory Properties of Cookies Produced by Partial Substitution of Margarine with Avocado Pear (Persia americana)

2021 ◽  
Vol 13 (1) ◽  
pp. 96-104
Author(s):  
Jelili Babatunde Hussein ◽  
Joseph Idowu Olaniyi ◽  
Esther Anjikwi Msheliza ◽  
Seember Bernadette Kave
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Iodine Value ◽  
Peroxide Value ◽  
Crude Protein ◽  
The Other ◽  
Partial Substitution ◽  
Standard Methods ◽  
Physico Chemical ◽  
Sensory Qualities

The partial substitution of margarine with mature avocado pear pulp in the production of cookies was investigated. Five cookie samples were produced with avocado pear pulp and margarine blends in the ratios 80:20%, 70:30%, 60:40%, 50:50%, and 100% margarine serve as the control, labeled as B, C, D and E and A, respectively while the other ingredients used remain constant. The physico-chemical (proximate compositions, free fatty acids (FFA), iodine value, and peroxide value) and sensory qualities of the cookies were evaluated using standard methods. The results show ranged in moisture (11.13 to 14.60%), crude protein (6.93 to 7.83%), crude fat (16.00 to 18.03%), ash (1.40 to 2.09%), crude fiber (0.29 to 0.62%), carbohydrate (59.70 to 62.79%), FFA (0.35 to 1.01 mg KOH/g), iodine value (75.63 to 81.17 g I2/100 g) and peroxide value (2.96 to 5.27 meq/kg). The partial substitution of margarine with avocado pear pulp produced nutritious cookies with desirable organoleptic qualities. Also, the results demonstrated that cookies had acceptability up to a 30% level of substitution with avocado pear pulp. The findings indicated the feasibility of avocado pear pulp in fat-reduced cookies preparation, this will reduce the pressure in using only margarine in cookies making and diversify the use of avocado pear.

scholarly journals The effect of grape ripening stage on red wine color

OENO One ◽  
2006 ◽  
Vol 40 (1) ◽  
pp. 15 ◽  
Author(s):  
Ana Belén Bautista-Ortín ◽  
José Ignacio Fernández-Fernández ◽  
José María López-Roca ◽  
Encarnación Gómez-Plaza
Keyword(s):  
Red Wine ◽  
Vitis Vinifera L ◽  
Cell Wall Degradation ◽  
Color Intensity ◽  
Fresh Weight ◽  
Physico Chemical ◽  
Yellow Color ◽  
Anthocyanin Concentration ◽  
Wine Color

<p style="text-align: justify;">The physico-chemical and chromatic characteristics of grapes (Vitis vinifera L. cv. Monastrell) harvested at six different degree of ripeness (from August 16 to October 24, 2002) and that of the wines obtained from these grapes have been studied. The grape anthocyanins content (mg/kg of berry fresh weight) was maximum in those grapes harvested on September 11 and 16 (804.1 and 822.6 mg/kg, respectively) and decreased for grapes harvested in October. However, the results showed that the grapes with the highest anthocyanin concentration did not lead to the highest colored wines. The wines elaborated from grapes harvested on October 16 (671.9 mg of anthocyanins per kg of berry fresh weight) had the best chromatic characteristics and better withstood aging in the bottle; the extent of cell wall degradation in overly matured grapes probably facilitated the extraction of phenolic compounds from skins. However, the chromatic quality of wines made from grapes harvested one week later (October 24, the most mature grapes) was lower than that from October 16, with lower color intensity (13%lower in the wine elaborated from grapes harvested in October 24) and a percentage of yellow color 6 % higher in this wine.</p>

scholarly journals The clinical cross-reactivity and immunological cross-antigenicity of wheat and barley

2021 ◽  
Author(s):  
Shohei Kubota ◽  
Yuji Aoki ◽  
Tomomi Sskai ◽  
Katsumasa Kitamura ◽  
Teruaki Matsui ◽  
...  
Keyword(s):  
Oral Immunotherapy ◽  
Cross Reactivity ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wheat Allergy ◽  
Extract Concentration ◽  
Low Concentrations ◽  
High Concentrations ◽  
Food Challenges ◽  
Elisa Inhibition

Background: Some patients with a wheat allergy have been reported to show clinical cross-reactivity to barley. However, it is not clear whether the development of barley allergy in patients with a wheat allergy is due to cross-antigenicity between wheat and barley. In our study, we aimed to determine the clinical cross-reactivity and immunological cross-antigenicity of wheat and barley. Methods: We compared the results of barley oral food challenges (OFCs) before oral immunotherapy (OIT) for wheat with those after OIT in nine patients with a wheat allergy to estimate the clinical cross-reactivity of wheat and barley. Moreover, we performed enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblotting inhibition using serum from seven patients allergic to wheat and barley. Results: Nine patients who had positive barley-OFC results performed before OIT for wheat were all negative on barley-OFC performed after OIT. In ELISA inhibition, preincubation of serum from patients allergic to wheat and barley with a high barley extract concentration inhibited binding of IgE to wheat extract by less than 10%. On the other hand, wheat and barley extracts equally inhibited binding to barley sIgE at high concentrations. In the immunoblotting inhibition test, the spots of wheat were inhibited but weakly by barley extracts, and most of the spots of barley were inhibited even by low concentrations of the wheat and barley extract. Conclusion: We showed that barley allergy associated with wheat allergy is caused by cross-reactivity from wheat. The OIT for wheat was one of the promising options for barley allergy.

Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Mass Spectrometry ◽  
Dry Weight ◽  
Paper Electrophoresis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Hordeum Vulgare L ◽  
Layer Chromatography

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.

Inhibition of δ-Aminolevulinic Acid Synthesis by Glyphosate

Weed Science ◽  
1981 ◽  
Vol 29 (5) ◽  
pp. 571-577 ◽  
Author(s):  
Lynn M. Kitchen ◽  
William W. Witt ◽  
Charles E. Rieck
Keyword(s):  
Carbon Dioxide ◽  
Zea Mays ◽  
Aminolevulinic Acid ◽  
Higher Plants ◽  
Acid Synthesis ◽  
The Other ◽  
Porphyrin Ring ◽  
Fresh Weight ◽  
Ring Compounds ◽  
Site Of Action

The effect of glyphosate [N-(phosphonomethyl) glycine] on barley (Hordeum vulgareL.) and corn (Zea maysL.) shoot δ-aminolevulinic acid (ALA) production was examined by monitoring ALA content in the tissue and measuring incorporation of14C precursors into ALA and chlorophylla. Barley shoot ALA content was significantly decreased by 1 mM glyphosate after 9, 11, and 15 h of illumination. ALA production by treated barley shoots was 30 nmoles•g fresh weight-1•h-1at each interval tested, compared with 75 to 120 nmoles•g fresh weight-1•h-1for the control. In corn shoots, ALA content was reduced 32, 45, and 58% by 0.1, 1.0, and 10.0 mM glyphosate, respectively, after 12 h illumination. Incorporation studies with14C-glutamate,14C-α-ketoglutarate, and14C-glycine into ALA showed a 77, 92, and 91% inhibition, respectively, in barley shoots treated with 1 mM glyphosate. Incorporation of14C-ALA into chlorophyllawas not affected by 1 mM glyphosate. Thus, the site of action of glyphosate may involve two enzyme pathways:one controlling the conversion of α-ketoglutarate to ALA, and the other controlling the condensation of glycine with succinyl CoA to form ALA and carbon dioxide. Inhibition of ALA synthesis blocks synthesis of chlorophyll, as well as all other porphyrin ring compounds found in higher plants. Thus, inhibition of ALA synthesis may be an integral component of the herbicidal mode of action of glyphosate.

JUSTIFICATION OF VETERINARY AND SANITARY ASSESSMENT OF WILD AND DOMESTIC DUCKS AT SARCOCYSTOSIS

2019 ◽  
Vol 1 (1) ◽  
pp. 6-12
Author(s):  
I. G. Seregin ◽  
◽  
Yu. V. Petrova ◽  
S. V. Redkin ◽  
Yu. G. Isaev ◽  
...  
Keyword(s):  
The Other ◽  
Internal Organs ◽  
Domestic Ducks ◽  
Wild Duck ◽  
Central Russia ◽  
Physico Chemical ◽  
Muscle Lesions ◽  
Canning Industry ◽  
Microbiological Indicators

The article presents the results of studies of carcasses of domestic and wild ducks for the purpose of detection of sarcocystosis. The commodity, organoleptic, physico-chemical, microbiological indicators of the meat of sick and healthy domestic and wild ducks were determined. Researches were carried out in spring and autumn in the farms of six regions of Central Russia, on the territory of which there were large reservoirs (ponds, lakes or swamps). The intensity of the destruction of wild ducks by sarcocystosis was high, of domestic ducks was medium. Sarcocystis one wild duck found in large quantity in the chest muscles and lower in the muscles of the neck, the other two (domestic and wild) affected ducks Sarcocystis was discovered in many skeleton, which muscles. In chest muscles of one wild duck sarcocystis has revealed in large quantity and lower in the muscles of the neck, sarcocystis was discovered in many muscles of skeleton of the other two (domestic and wild) affected ducks. The size of the sarcocystosis was different, but no more than the millet grain. In our opinion, in cases of intense muscle lesions with sarcocistosis, carcasses and poultry organs should be sent for disposal or fed to animals after stripping and boiling for at least 1 hour. At weak injury and the absence of dystrophic processes in the muscles of the carcass, their internal organs are cleaned and sent to the canning industry. It is not allowed to feed infected sarcocistosis raw bird meat to dogs, cats and other carnivores.

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