scholarly journals Deciphering Morphological Changes in a Sinuous River System by Higher-Order Velocity Moments

Water ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 772 ◽  
Author(s):  
Jyotismita Taye ◽  
Jyotirmoy Barman ◽  
Bimlesh Kumar ◽  
Giuseppe Oliveto
Keyword(s):  
Velocity Fluctuation ◽  
Morphological Changes ◽  
River System ◽  
Streamwise Velocity ◽  
Flume Experiments ◽  
Velocity Fluctuations ◽  
Alluvial Channel ◽  
Even Order ◽  
Sinuous Channel ◽  
And Migration

Bank erosion in a sinuous alluvial channel is a continuous phenomenon resulting in bank instability and migration of sediment. In this study, flume experiments were conducted in a sinuous channel to investigate its morphological changes and hydrodynamics. High-order velocity fluctuation moments are analyzed at outer and inner banks to explain the morphological variation in a sinuous river channel. The variance of streamwise velocity fluctuations on both banks of the sinuous channel follows a logarithmic law from a particular depth. In the outer bend region, the magnitude of velocity fluctuation moment is significant, indicating erosion. The trend of velocity fluctuation at higher even-order moments is similar to the variance of streamwise velocity fluctuations where the outer bend magnitude is greater than the inner bend. The premultiplied probability density functions (PDFs) and the flatness factor show greater magnitude in the outer bend of the channel as compared to the inner bend.

High-Order Velocity Moments of Turbulent Boundary Layers in Seepage Affected Alluvial Channel

2018 ◽  
Vol 140 (8) ◽  
Author(s):  
Anurag Sharma ◽  
Bimlesh Kumar
Keyword(s):  
Boundary Layer ◽  
Sediment Transport ◽  
Turbulent Boundary Layer ◽  
Seepage Flow ◽  
Streamwise Velocity ◽  
High Order ◽  
Alluvial Channel ◽  
Even Order ◽  
Logarithmic Law ◽  
Velocity Moments

In this work, we have performed the flume study to analyze the high-order velocity moments of turbulent boundary layer with and without downward seepage. Sediment transport experiments were done in the laboratory for no seepage (NS), 10% seepage (10%S), and 15% seepage (15%S) cases. Measures of streamwise velocity variance were found increasing with seepage, which lead to increase in sediment transport with seepage. Results show that the variance of streamwise velocity fluctuation follows logarithmic law with distance away from the bed, within inner layer. This observation is also valid for even-order moments obtained in this work. The results show that the (2p-order moments)1/p also follows logarithmic law. The slopes Ap in the turbulent boundary layer seem fairly unaffected to NS and seepage flow but follows nonuniversal behavior for NS and seepage runs. The computed slope based on the Gaussian statistics does not agree well with the slope obtained from the experimental data and computed slope are reliable with sub-Gaussian performance for NS flow and super-Gaussian behavior for seepage flow.

scholarly journals Mesothelin blockage by Amatuximab suppresses cell invasiveness, enhances gemcitabine sensitivity and regulates cancer cell stemness in mesothelin-positive pancreatic cancer cells

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fumihiko Matsuzawa ◽  
Hirofumi Kamachi ◽  
Tatsuzo Mizukami ◽  
Takahiro Einama ◽  
Futoshi Kawamata ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Morphological Changes ◽  
Pancreatic Cancer Cells ◽  
Cell Invasiveness ◽  
And Migration ◽  
Migration Capacity

Abstract Background Mesothelin is a 40-kDa glycoprotein that is highly overexpressed in various types of cancers, however molecular mechanism of mesothelin has not been well-known. Amatuximab is a chimeric monoclonal IgG1/k antibody targeting mesothelin. We recently demonstrated that the combine therapy of Amatuximab and gemcitabine was effective for peritonitis of pancreatic cancer in mouse model. Methods We discover the role and potential mechanism of mesothelin blockage by Amatuximab in human pancreatic cells both expressing high or low level of mesothelin in vitro experiment and peritonitis mouse model of pancreatic cancer. Results Mesothelin blockage by Amatuximab lead to suppression of invasiveness and migration capacity in AsPC-1 and Capan-2 (high mesothelin expression) and reduce levels of pMET expression. The combination of Amatuximab and gemcitabine suppressed proliferation of AsPC-1 and Capan-2 more strongly than gemcitabine alone. These phenomena were not observed in Panc-1 and MIA Paca-2 (Mesothelin low expression). We previously demonstrated that Amatuximab reduced the peritoneal mass in mouse AsPC-1 peritonitis model and induced sherbet-like cancer cell aggregates, which were vanished by gemcitabine. In this study, we showed that the cancer stem cell related molecule such as ALDH1, CD44, c-MET, as well as proliferation related molecules, were suppressed in sherbet-like aggregates, but once sherbet-like aggregates attached to peritoneum, they expressed these molecules strongly without the morphological changes. Conclusions Our work suggested that Amatuximab inhibits the adhesion of cancer cells to peritoneum and suppresses the stemness and viability of those, that lead to enhance the sensitivity for gemcitabine.

Larger-Eddy Simulation of Velocity Fluctuation in a Mixing T-Junction

2012 ◽  
Vol 152-154 ◽  
pp. 1313-1318
Author(s):  
Tao Lu ◽  
Su Mei Liu ◽  
Ping Wang ◽  
Wei Yyu Zhu
Keyword(s):  
Experimental Data ◽  
Velocity Fluctuation ◽  
Flow Model ◽  
Numerical Results ◽  
Mean Square ◽  
Main Duct ◽  
Velocity Fluctuations ◽  
Eddy Simulation ◽  
Large Eddy ◽  
Les Model

Velocity fluctuations in a mixing T-junction were simulated in FLUENT using large-eddy simulation (LES) turbulent flow model with sub-grid scale (SGS) Smagorinsky–Lilly (SL) model. The normalized mean and root mean square velocities are used to describe the time-averaged velocities and the velocities fluctuation intensities. Comparison of the numerical results with experimental data shows that the LES model is valid for predicting the flow of mixing in a T-junction junction. The numerical results reveal the velocity distributions and fluctuations are basically symmetrical and the fluctuation at the upstream of the downstream of the main duct is stronger than that at the downstream of the downstream of the main duct.

Distinct signals via Rho GTPases and Src drive shape changes by thrombin and sphingosine-1-phosphate in endothelial cells

2002 ◽  
Vol 115 (12) ◽  
pp. 2475-2484 ◽  
Author(s):  
Valérie Vouret-Craviari ◽  
Christine Bourcier ◽  
Etienne Boulter ◽  
Ellen Van Obberghen-Schilling
Keyword(s):  
Endothelial Cells ◽  
Rho Gtpases ◽  
Actin Polymerization ◽  
Morphological Changes ◽  
Umbilical Vein ◽  
Cell Spreading ◽  
Actin Binding ◽  
Sphingosine 1 Phosphate ◽  
And Migration ◽  
Umbilical Vein Endothelial Cells

Soluble mediators such as thrombin and sphingosine-1-phosphate regulate morphological changes in endothelial cells that affect vascular permeability and new blood vessel formation. Although these ligands activate a similar set of heterotrimeric G proteins, thrombin causes cell contraction and rounding whereas sphingosine-1-phosphate induces cell spreading and migration. A functional requirement for Rho family GTPases in the cytoskeletal responses to both ligands has been established, yet the dynamics of their regulation and additional signaling mechanisms that lead to such opposite effects remain poorly understood. Using a pull-down assay to monitor the activity of Rho GTPases in human umbilical vein endothelial cells, we find significant temporal and quantitative differences in RhoA and Rac1 activation. High levels of active RhoA rapidly accumulate in cells in response to thrombin whereas Rac1 is inhibited. In contrast, sphingosine-1-phosphate addition leads to comparatively weak and delayed activation of RhoA and it activates Rac1. In addition, we show here that sphingosine-1-phosphate treatment activates a Src family kinase and triggers recruitment of the F-actin-binding protein cortactin to sites of actin polymerization at the rim of membrane ruffles. Both Src and Rac pathways are essential for lamellipodia targeting of cortactin. Further, Src plays a determinant role in sphingosine-1-phosphate-induced cell spreading and migration. Taken together these data demonstrate that the thrombin-induced contractile and immobile phenotype in endothelial cells reflects both robust RhoA activation and Rac inhibition, whereas Src- and Rac-dependent events couple sphingosine-1-phosphate receptors to the actin polymerizing machinery that drives the extension of lamellipodia and cell migration.

scholarly journals Flume experiments on vegetated alternate bars

2018 ◽  
Vol 40 ◽  
pp. 02034 ◽  
Author(s):  
Giulio Calvani ◽  
Simona Francalanci ◽  
Luca Solari
Keyword(s):  
Hydrological Regime ◽  
Spatial Arrangement ◽  
Flume Experiments ◽  
Rigid Vegetation ◽  
Hydraulic Conditions ◽  
Laboratory Flume ◽  
Alternate Bars ◽  
River Reach ◽  
Bed Slope ◽  
And Migration

The planform morphology of a river reach is the result of the combined actions of sediment motion (erosion, transport and deposition), hydrological regime, development and growth of vegetation. However, the interactions among these processes are still poorly understood and rarely investigated in laboratory flume experiments. In these experiments and also in numerical modelling, vegetation is usually represented by rigid cylinders, although it is widely recognized that this schematization cannot reproduce the effects of root stabilization and binding on riverbed sediment. In this work, we focus on the effects of added vegetation on morphological dynamics of alternate bars in a straight channel by means of flume experiments. We performed laboratory experiments reproducing hydraulic conditions that are typical of gravel bed rivers, in terms of water depth, bed slope and bed load; these conditions led to the formation of freely migrating alternate bars. We then employed rigid vegetation that was deployed on the reproduced alternate bars according to field observations. Various vegetation scenarios, in terms of density and spatial arrangement, were deployed in the flume experiments such to mimic different maintenance strategies. Results show the effects of rigid vegetation on the alternate bar configuration on the overall topographic pattern, the main alternate bar characteristics (such as amplitude and wavelength) and migration rate.

scholarly journals Turbulence statistics in smooth wall oscillatory boundary layer flow

2018 ◽  
Vol 849 ◽  
pp. 192-230 ◽  
Author(s):  
Dominic A. van der A ◽  
Pietro Scandura ◽  
Tom O’Donoghue
Keyword(s):  
Boundary Layer ◽  
Normal Distribution ◽  
Boundary Layer Flow ◽  
Vertical Gradient ◽  
Streamwise Velocity ◽  
Normal Velocity ◽  
Half Cycle ◽  
Velocity Fluctuations ◽  
Oscillatory Boundary Layer ◽  
Layer Flow

Turbulence characteristics of an asymmetric oscillatory boundary layer flow are analysed through two-component laser-Doppler measurements carried out in a large oscillatory flow tunnel and direct numerical simulation (DNS). Five different Reynolds numbers, $R_{\unicode[STIX]{x1D6FF}}$, in the range 846–2057 have been investigated experimentally, where $R_{\unicode[STIX]{x1D6FF}}=\tilde{u} _{0max}\unicode[STIX]{x1D6FF}/\unicode[STIX]{x1D708}$ with $\tilde{u} _{0max}$ the maximum oscillatory velocity in the irrotational region, $\unicode[STIX]{x1D6FF}$ the Stokes length and $\unicode[STIX]{x1D708}$ the fluid kinematic viscosity. DNS has been carried out for the lowest three $R_{\unicode[STIX]{x1D6FF}}$ equal to 846, 1155 and 1475. Both experimental and numerical results show that the flow statistics increase during accelerating phases of the flow and especially at times of transition to turbulent flow. Once turbulence is fully developed, the near-wall statistics remain almost constant until the late half-cycle, with values close to those reported for steady wall-bounded flows. The higher-order statistics reach large values within a normalized wall distance of approximately $y/\unicode[STIX]{x1D6FF}=0.2$ at phases corresponding to the onset of low-speed streak breaking, because of the intermittency of the velocity fluctuations at these times. In particular, the flatness of the streamwise velocity fluctuations reaches values of the order of ten, while the flatness of the wall-normal velocity fluctuations reaches values of several hundreds. Far from the wall, at locations where the vertical gradient of the streamwise velocity is zero, the skewness is approximately zero and the flatness is approximately equal to 3, representative of a normal distribution. At lower elevations the distribution of the fluctuations deviate substantially from a normal distribution, but are found to be well described by other standard theoretical probability distributions.

Effects of episodic sediment supply on experimental step-pool channel morphology, bedload transport and channel stability

2021 ◽  
Author(s):  
Jiamei Wang ◽  
Marwan A. Hassan ◽  
Matteo Saletti ◽  
Xingyu Chen ◽  
Xudong Fu ◽  
...  
Keyword(s):  
Grain Size ◽  
Morphological Changes ◽  
Mass Movement ◽  
Channel Morphology ◽  
Bedload Transport ◽  
Sediment Supply ◽  
Primary Control ◽  
Channel Stability ◽  
Flume Experiments ◽  
Small Magnitude

<p>Steep step-pool streams are often coupled to adjacent hillslope, directly receiving episodic sediment supply from mass movement processes such as landslides and debris flows. The response of step-pool channels to the variations in sediment supply remains largely unexplored. We conducted flume experiments with a poorly sorted grain-size distribution in an 8%-steep, 5-m long flume with variable width at the University of British Columbia, to study the effects of episodic sediment supply on channel evolution. After a conditioning phase with no feed, the channel was subjected to sediment pulses of different magnitude and frequency under constant flow discharge. High-resolution data of hydraulics, bedload transport, bed surface grain size, and channel morphology were collected every 10-20 minutes and an additional time at the end of each pulse.</p><p>In response to sediment pulses, we recorded an increase in bedload transport rates, channel aggradation, bed surface fining, and continuous step formation and collapse. In between pulses, bedload rates dropped by several orders of magnitude, net erosion occurred, the bed surface gradually coarsened, and steps became more stable. The small-magnitude high-frequency pulses caused smaller but more frequent spikes in bedload transport, bed surface evolution, and thus step stability. Instead, the large-magnitude low-frequency pulses cause larger changes but provided a longer time for the channel to recover. This suggests that in step-pool channels pulse magnitude is a key control on channel rearrangement, while pulse frequency controls how fast and strong the recovery is.</p><p>The frequency and stability of steps varied as a function of local channel width, showing that channel geometry is a primary control on step formation and stability even under episodic sediment supply conditions. Instead, the effect of sediment pulses is less important because the total number and average survival time of steps were similar among runs with different pulses. The critical Shields stress decreased following sediment pulses, then increased immediately after, and fluctuated until the next pulse. The variations in sediment supply caused cycles in bedload transport rate, surface and bedload texture, thus controlling the variability in the threshold for motion.</p><p>Our results indicate that episodic sediment supply is a primary control on the evolution of step-pool channels, with sediment feed magnitude affecting mostly morphological changes, and sediment feed frequency controlling channel stability.</p>

scholarly journals Performance of a groyne in controlling flow, sediment and morphology around a tributary confluence

2018 ◽  
Vol 40 ◽  
pp. 04006
Author(s):  
Kohji Michioku ◽  
Yuki Osawa ◽  
Keiichi Kanda
Keyword(s):  
Morphological Changes ◽  
Main Channel ◽  
Original Position ◽  
River Morphology ◽  
Excellent Performance ◽  
Middle Stream ◽  
Sedimentary Deposit ◽  
And Migration ◽  
Flood Flow

In a middle stream reach, irreversible morphological changes are observed such as growth of a huge sandbar in front of the confluence, thalweg migration from the left to right bank, erosion of the main channel and sedimentary deposit on the floodplain. As a countermeasure against such degradation of river morphology, a groyne was constructed beneath the tributary confluence. Performance of a groyne in controlling flood flow, sediment transport and river morphology was investigated by a twodimensional hydrodynamic model. The analysis on twenty years of morphological change indicates that the groyne has an excellent performance in improving quality of river morphology such as reduction of the sandbar development and migration of the thalweg to the original position.

scholarly journals Natural logarithms

2013 ◽  
Vol 718 ◽  
pp. 1-4 ◽  
Author(s):  
B. J. McKeon
Keyword(s):  
Turbulent Flows ◽  
Reynolds Numbers ◽  
Streamwise Velocity ◽  
Wall Turbulence ◽  
Significant Advance ◽  
Mean Velocity ◽  
Velocity Fluctuations ◽  
Logarithmic Scaling ◽  
The Mean ◽  
High Reynolds

AbstractMarusic et al. (J. Fluid Mech., vol. 716, 2013, R3) show the first clear evidence of universal logarithmic scaling emerging naturally (and simultaneously) in the mean velocity and the intensity of the streamwise velocity fluctuations about that mean in canonical turbulent flows near walls. These observations represent a significant advance in understanding of the behaviour of wall turbulence at high Reynolds number, but perhaps the most exciting implication of the experimental results lies in the agreement with the predictions of such scaling from a model introduced by Townsend (J. Fluid Mech., vol. 11, 1961, pp. 97–120), commonly termed the attached eddy hypothesis. The elegantly simple, yet powerful, study by Marusic et al. should spark further investigation of the behaviour of all fluctuating velocity components at high Reynolds numbers and the outstanding predictions of the attached eddy hypothesis.

Flame Response Mechanisms Due to Velocity Perturbations in a Lean Premixed Gas Turbine Combustor

2010 ◽  
Vol 133 (2) ◽  
Author(s):  
Brian Jones ◽  
Jong Guen Lee ◽  
Bryan D. Quay ◽  
Domenic A. Santavicca
Keyword(s):  
Transfer Function ◽  
Heat Release ◽  
Gas Turbine ◽  
Velocity Fluctuation ◽  
Gas Turbine Combustor ◽  
Velocity Fluctuations ◽  
Inlet Velocity ◽  
Lean Premixed ◽  
Flame Response ◽  
Second Peak

The response of turbulent premixed flames to inlet velocity fluctuations is studied experimentally in a lean premixed, swirl-stabilized, gas turbine combustor. Overall chemiluminescence intensity is used as a measure of the fluctuations in the flame’s global heat release rate, and hot wire anemometry is used to measure the inlet velocity fluctuations. Tests are conducted over a range of mean inlet velocities, equivalence ratios, and velocity fluctuation frequencies, while the normalized inlet velocity fluctuation (V′/Vmean) is fixed at 5% to ensure linear flame response over the employed modulation frequency range. The measurements are used to calculate a flame transfer function relating the velocity fluctuation to the heat release fluctuation as a function of the velocity fluctuation frequency. At low frequency, the gain of the flame transfer function increases with increasing frequency to a peak value greater than 1. As the frequency is further increased, the gain decreases to a minimum value, followed by a second smaller peak. The frequencies at which the gain is minimum and achieves its second peak are found to depend on the convection time scale and the flame’s characteristic length scale. Phase-synchronized CH∗ chemiluminescence imaging is used to characterize the flame’s response to inlet velocity fluctuations. The observed flame response can be explained in terms of the interaction of two flame perturbation mechanisms, one originating at flame-anchoring point and propagating along the flame front and the other from vorticity field generated in the outer shear layer in the annular mixing section. An analysis of the phase-synchronized flame images show that when both perturbations arrive at the flame at the same time (or phase), they constructively interfere, producing the second peak observed in the gain curves. When the perturbations arrive at the flame 180 degrees out-of-phase, they destructively interfere, producing the observed minimum in the gain curve.

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