scholarly journals Influence of a new generation adaptogen and probiotic combination on sheep phagocytosis indicators

2020 ◽  
Author(s):  
N.V. Belova ◽  
Keyword(s):  
Phagocytic Activity ◽  
Specific Resistance ◽  
Phagocytic Index ◽  
Blood Leukocytes ◽  
Immunological Status ◽  
Probiotic Lactobacilli ◽  
New Generation ◽  
Positive Effect

The purpose of the research was to investigate the effect of the lithium ascorbate stress-protector and its complex with a probiotic based on lactobacilli on the phagocytic activity of blood leukocytes. In the course of this research, we found that lithium ascorbate stress-protector combined with probiotic lactobacilli strains reliably increases the phagocytic index of blood by 28.6 % and has a positive effect on the non-specific resistance and immunological status of sheep.

scholarly journals Use of probiotic lactobacilli to increase non-specific resistance and productivity of rabbits

2020 ◽  
Author(s):  
A.N. Ovcharova ◽  
Keyword(s):  
Growth Rate ◽  
Bactericidal Activity ◽  
Phagocytic Activity ◽  
Specific Resistance ◽  
Live Weight ◽  
Phagocytic Index ◽  
Control Group ◽  
Control Parameters ◽  
Probiotic Lactobacilli ◽  
Experimental Group

The article presents the results of the effectiveness of probiotic lactobacilli on the productivity and non-specific resistance of rabbits. The phagocytic activity of neutrophils increased by 10 %, which turns out to be statistically significant. The bactericidal activity of blood increased by 8 % in comparison with the control group; the phagocytic index was also higher in the experimental group by 2.02. The content of lysozyme in the blood serum of rabbits in the experimental group was significantly higher than the control parameters by 18.6 micrograms/ml. As tentative results, we report an increase in the live weight and growth rate in the experimental group of rabbits.

CHANGES IN THE GENEALOGICAL STRUCTURE OF THE BREEDING STOCK OF THE YAROSLAVL BREED IN THE VOLOGDA REGION

2020 ◽  
pp. 24-29
Author(s):  
N.I. ABRAMOVA ◽  
G.S. VLASOVA ◽  
O.L. KHROMOVA
Keyword(s):  
Protein Content ◽  
Negative Impact ◽  
Breeding Stock ◽  
Holstein Breed ◽  
Vologda Region ◽  
Ideal Line ◽  
Productivity Potential ◽  
New Generation ◽  
Modern Population ◽  
Positive Effect

Проведен сопоставительный анализ генеалогической структуры племенного поголовья ярославской породы Вологодской области по данным 2005 и 2018 годов. Установлено, что в 2005 году в структуру породы входили 8 отечественных генеалогических линий. В результате скрещивания с голштинской породой произошли значительные изменения. Доля животных, принадлежащих к линиям ярославской породы, уменьшилась со 100% в 2005 году до 35,3% в 2018 году. В генеалогическую структуру современной популяции добавились 3 линии голштинской селекции, к которым на 1.01.2019 года относится большая часть маточного поголовья: к линии Рефлекшн Соверинга 198998 — 42,9%, Вис Бэк Айдиала 1013415 — 13%, Монтвик Чифтейна 95679 — 8,8%. Скрещивание с голштинской породой оказало положительное влияние на повышение молочной продуктивности животных ярославской породы. Средний надой на 1 корову увеличился на 1670 кг молока, доля коров с продуктивностью более 6000 кг молока выросла на 35%. В результате расчета корреляционной зависимости между кровностью по голштинской породе и массовой долей жира и белка в молоке установлено, что скрещивание не ухудшило качественные показатели молока коров ярославской породы (степень кровности с МДЖ — r=-0,01; с МДБ — r=0,09; P<0,01), но в то же время отразилось отрицательно на продолжительности хозяйственного использования животных в стаде (r=-0,27; P<0,001). Для дальнейшего совершенствования породы следует определить оптимальную степень прилития крови голштинской породы. А с целью сохранения генофонда необходимо получение нового поколения чистопородных быков ярославской породы с более высоким потенциалом продуктивности. The authors conduct a comparative analysis of the genealogical structure of the breeding stock of the Yaroslavl breed in the Vologda region on the basis of the data for 2005 and 2018. It is established that in 2005 the structure of the breed included 8 domestic genealogical lines. As a result of the crossing with the Holstein breed, significant changes occurred. The share of animals of the Yaroslavl breed lines decreased from 100% in 2005 to 35.3% in 2018. The genealogical structure of the modern population got 3 added lines of the Holstein selection which as of 1.01.2019 include most of the breeding stock: Reflection Sovering line 198998 - 42.9%, Vis Back Ideal line 1013415 - 13%, and Montwick Chieftain line 95679 - 8.8%. The crossing with the Holstein breed had a positive effect on the increase in the milk productivity of the Yaroslavl breed animals. The average milk yield per 1 cow increased by 1670 kg of milk and the share of cows with the productivity of more than 6000 kg of milk increased by 35%. The results of the calculations of the correlation between the Holstein thoroughbredness and the fat and protein content in the milk showed that the crossing did not impair the quality indicators of the milk the Yaroslavl breed cows (thoroughbredness degree with the fat content r=-0.01; with the protein content r=0.09 P<0.01), but at the same time had a negative impact on the duration of the economic use of the animals in the herd (r=-0.27; P<0.001). For further improvement of the breed, there is a need to determine the optimal degree of crossing with the Holstein breed. Also, in order to preserve the gene pool, there is a need to obtain a new generation of purebred bulls of the Yaroslavl breed with a higher productivity potential.

Natural resistance of marals of Altai-Sayan breed during the process of adaptation under the environments of the Republic of Tyva

2020 ◽  
pp. 41-49
Author(s):  
Ch. N. Sambyla ◽  
N. M. Bessonova ◽  
R. B. Chysyma
Keyword(s):  
Cervus Elaphus ◽  
Phagocytic Activity ◽  
Natural Habitat ◽  
Reproductive Function ◽  
Climatic Conditions ◽  
Natural Resistance ◽  
Phagocytic Index ◽  
Mountain Forest ◽  
Altai Republic ◽  
The Republic

The Republic of Tyva is a region in the geographical center of Asia, which located at the junction of the Siberian taiga and Central Asian desert-steppe landscapes, in a wide band of mountains and intermountain plains. The mountain-forest area of Tyva has long been considered a natural habitat for antler deer, one of which is the maral (Cervus elaphus sibiricus). In order to restore maral breeding and increase the number of marals in the former limits, marals of the Altai-Sayan breed have been imported to the Republic of Tyva from the Republic of Altai. The preservation of productive traits, reproductive function and the realization of the genetic potential of animals introduced to new climatic conditions largely depends on the ability of these animals to adapt to existing environments. We have assessed in this paper the natural resistance of the marals of Altai-Sayan breed during introduction in the Tyva Republic in comparison with the indicators of the marals have been bred in the Altai Republic. The researches have been carried out in 2019. The research material was blood samples of marals of Altai-Sayan breed imported to the Republic of Tuva (n=27) and marals of the same breed bred in the Republic of Altai (n=17). Studies have shown some deviations in the blood leukogram of imported marals, such as a decrease in the number of eosinophils and rod nuclear neutrophils (P < 0,001), the increase in the number of segmented nuclear neutrophils and lymphocytes (P < 0,05). The number of monocytes have been increased in 18,6 times compared to the Altai marals. The change in the number of monocytes exceeded the physiological norm by 24,7 %. The indicator of adaptation evaluation in imported marals had higher values (6,8), which were in 1,7 times higher than in marals bred in the Altai Republic (4,1), which indicates the intensity of adaptive mechanisms in imported animals during adaptation. Analysis of phagocytic activity and phagocytic index revealed intensive phagocytosis in imported marals: phagocytic activity – by 12,4 % (P < 0,05), phagocytic index – by 5,1 %, and the increase in the content of lysosomal and cationic proteins by 12,8 % (P < 0,05). Analysis of the bactericidal activity of blood serum has shown its lower level in imported animals (45,97±1,36 %), compared with marals of the same breed bred in the Altai Republic (52,19±2,15 at P < 0,05). Thus, according to most indicators of natural resistance marals of Altai-Sayan breed imported to Tyva have a fairly high level of natural protection, which indicates that they can be adapted to the natural climatic and feeding conditions of the Republic of Tyva.

scholarly journals The cell protection of weated pigs for probiotics

2020 ◽  
pp. 111-119
Author(s):  
L. Bondarenko
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
The State ◽  
Natural Resistance ◽  
Phagocytic Index ◽  
Control Group ◽  
Stressful Situation ◽  
Experimental Group

The immune system is central to ensuring the consistency of the body's homeostasis. The state of the body's natural resistance is determined by a set of non-specific protective mechanisms. Lymphocytes and phagocytes are actively involved in maintaining immunity. Lymphocytes recognize the antigens of pathogenic microorganisms, and phagocytes absorb and destroy the pathogens themselves. During the weaning of piglets from sows there is a decrease in the protective forces of their body. During this period, the natural resistance of the piglets is reduced due to the stressful situation caused by changing conditions of confinement, the transition to full feed and lack of sows. The immune system of weaning pigs is relatively weak, so when exposed to environmental and technological stressors, they become susceptible to various diseases. The use of probiotic drugs stimulates the activity of the immune system, prevents stress and immunodeficiency. One of these probiotics is the probiotic of domestic production Protecto-active. It w observed the the influence of the probiotic Protecto-active on the indices of nonspecific resistance of the young pigs organism to the growth. An increase in bactericidal activity of blood serum by 12.10% (P <0.05) and lysozyme activity of blood in the piglets of the experimental group was increased by 3.71% compared to control, which indicates the activation of the body's defenses and the increase in adaptive capacity. An important step in the study of the influence of the probiotic Protekto-active on the state of the immune system is to determine the phagocytic activity of neutrophils, phagocytic index and phagocytic number. In the experimental group of piglets that were fed the probiotic Protecto-active, we found an increase in leukocyte phagocytic activity by 9.0% (P <0.001), a phagocytic index by 51.7% (P<0.001) and a phagocytic number by 24.8% ( P <0.01) compared with the control group. Thus, using a probiotic Protecto-active, all indicators of phagocytosis increase: the number of phagocytes increases, their ability to capture microorganisms and increases their digestive capacity, it increases the bacterial and lysozyme activity of blood serum, which is positively reflected in the immunobiosity. Key words: probiotic, phagocytosis, phagocytic index, phagocytic number, phagocytic activity of leukocytes, cellular immunity, piglets

scholarly journals Перспективы применения гуминового комплекса на виноградниках Крыма

2019 ◽  
pp. 122-127
Author(s):  
Magometsaigit Beibulatov ◽  
Nadezhda Tikhomirova ◽  
Natalia Urdenko ◽  
Roman Buival ◽  
Yana Radionovskaya
Keyword(s):  
Positive Impact ◽  
Cabernet Sauvignon ◽  
Wine Grapes ◽  
Total Sugars ◽  
Table Grapes ◽  
New Generation ◽  
The Impact ◽  
Positive Effect ◽  
Average Cluster

В статье представлены результаты двухлетних исследований по изучению влияния внекорневых подкормок гуминовым препаратом нового поколения GSN-2004 HUMILIFEтм (ОСН-2004) на урожай и качество винограда столового сорта Мускат янтарный и технического сорта Каберне-Совиньон в условиях Южного берега Крыма. По результатам исследований 2017-2018 гг. установлено, что внекорневые подкормки изучаемым препаратом разработанной нормой 4 л/га, способствуют увеличению урожайности винограда сорта Мускат янтарный в среднем на 23,9%, при этом повышение урожайности составляет 2,7 т/га. Урожайность винограда сорта Каберне-Совиньон увеличивается на 26,5%, при этом повышение урожайности составляет 2,2 т/га. Подтверждено положительное влияние внекорневых подкормок на показатели качества винограда сорта Мускат янтарный, которое выразилось в увеличении средней массы грозди у изучаемого сорта на 40,3%, увеличении массовой концентрации сахаров в соке ягод на 5,4% и увеличении значений глюкоацидометрического показателя (ГАП) на 15,6%. Положительное влияние внекорневых подкормок на показатели качества винограда сорта Каберне-Совиньон выразилось в увеличении средней массы грозди на 23,0% и увеличении массовой концентрации сахаров в соке ягод на 7,1%.The paper summarizes findings of a two-year study on the impact of foliar fertilizing with a new generation humic preparation GSN-2004 HUMILIFEтм (ОСН-2004) on the harvest and quality of ‘Muscat Yantarniy’ table grapes and ‘Cabernet Sauvignon’ wine grapes in the conditions of the southern coast of Crimea. The data of the 2017-2018 study established that foliar dressing with the preparation under study in the ratio of 4 l/ha increases the harvest of ‘Muscat Yantarniy’ grapes on average by 23.9%, or by 2 t/ha. The yield of ‘Cabernet Sauvignon’ grapes was increased by 26.5%, thus increasing the harvest by 2.2 t/ha. The positive impact of foliar dressing on quality indicators of ‘Muscat Yantarniy’ grapes was confirmed. Thus, the average cluster weight increased by 40.3%, total sugars in the berry juice increased by 5.4 %, and gluco-acidometric index increased by 158.06 %. The study confirmed the positive effect of foliar dressing on quality characteristics of ‘Cabernet Sauvignon’ grapes, as demonstrated by a 23% increase in the average cluster weight and a 7.1 % increase in the total sugars in the berry juice.

scholarly journals Phagocytic activity of blood monocytes in response to methicillin-resistant strains of Staphylococcus aureus

2020 ◽  
Vol 10 (3) ◽  
pp. 551-557
Author(s):  
O. A. Kolenchukova ◽  
N. I. Sarmatova ◽  
A. V. Moshev
Keyword(s):  
Respiratory Burst ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Fluorescein Isothiocyanate ◽  
Phagocytic Index ◽  
Blood Monocytes ◽  
Monocyte Subsets ◽  
Superoxide Anion Production ◽  
Hla Dr ◽  
Burst Intensity

Current study performed to estimate the phagocytic activity of blood monocytes of varying phenotypes exposed to MRSA and MSSA strains.  Objects: Blood monocytes were collected from 25 healthy adults (age: 25–45 years). Live suspensions of MRSA/MSSA strains were used at concentration of 106 colony-forming units (CFU)/mL.  Metods. Phagocytic functions were estimated by using fluorescein isothiocyanate (FITC)-labelled MRSA and MSSA strains followed by running flow cytometry on FC 500 series flow cytometer (Beckman Coulter, USA). Whole peripheral blood cells were directly labelled with immunofluorescently tagged monoclonal CD14-PE/CD45-ECD/HLA-DR-PC5/CD16-PC7 antibodies (Beckman Coulter, USA). Respiratory burst intensity was evaluated in monocytes by measuring activity of lucigeninand luminol-dependent spontaneous and induced chemiluminescence. Monocytes were induced by using live suspension of MRSA/MSSA strains at a concentration of 106 CFU/mL. Results and discussion. While studying luminol-dependent monocyte activities after exposure to MRSA vs. MSSA, it was observed a 3.5-fold decreased curve square, whereas lucigenin-dependent chemiluminescence was increased by 6-fold. Compared to MSSA exposure, index of activation (IA) was decreased by 1.1-fold in response to MRSA exposure that was confirmed by lowered release of reactive oxygen species (ROS) from monocytes in response to MRSA exposure. Moreover, IRSS increased by 1.3-fold upon MRSA exposure. Examining monocyte oxygen-independent phagocytosis against MRSA vs. MSSA revealed significantly increased phagocytic number and concomitantly decreased phagocytic index. An evaluation of the activities of various monocyte subsets in response to MRSA vs. MSSA revealed increased phagocytic index by 1.5-fold for CD14lowCD16+ and CD14+CD16+ monocyte subsets as well as 3-fold for CD14+CD16– monocytes. Counts for all phagocytic subsets were decreased (1.4-, 1.5- and 4-fold for CD14lowCD16+, CD14+CD16+ and CD14+CD16– monocytes, respectively). To summarize, intensity of the respiratory burst was lowered upon MRSA exposure and percentage of monocyte subsets. Overall deficiency of superoxide anion production was observed in response to MRSA. In contrast, oxygen-independent event revealed phenotypic changes in frequency of peripheral blood monocytes upon MRSA exposure. We observed that CD14+CD16– classical monocytes were more rapidly activated. Conclusion. Thus, we concluded that CD14+CD16– monocytes became more rapidly activated but exhibited less effective phagocytosis, whereas CD14+CD16+ and CD14lowCD16+ monocytes were more slowly activated and demonstrated stronger phagocytic activity.

scholarly journals Increased leishmanicidal activity of alveolar macrophages from mature horses with mild equine asthma

2019 ◽  
Vol 71 (3) ◽  
pp. 939-943
Author(s):  
D.A.B. Lessa ◽  
N.X. Alencar ◽  
R.A. Torres Filho ◽  
M.F.M. Costa ◽  
W.R. Fernandes ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Alveolar Macrophages ◽  
Phagocytic Activity ◽  
Defense Mechanisms ◽  
Phagocytic Index ◽  
Leishmanicidal Activity ◽  
Phagocytic Ability ◽  
Survival Index ◽  
Mixed Breed ◽  
Equine Asthma

ABSTRACT Alveolar macrophages (AMs) are an essential part of defense mechanisms within the lungs and their phagocytic activity is important for organ homeostasis. The phagocytic ability of AMs obtained from bronchoalveolar lavage from 17 mature mixed-breed pleasure horses (8 healthy and 9 diagnosed with mild equine asthma) was studied through assays with Leishmania (Viannia) braziliensis promastigotes, which enabled the calculation of a phagocytic index (PI) and a survival index (SI). Results indicate that phagocytic activity of AMs in asthma affected horses is similar to healthy horses, while leishmanicidal activity is significantly increased in horses with asthma.

scholarly journals Genetic control of macrophage functions. I. Polygenic regulation of phagocytosis stimulation produced by Glyceryl Trioleate.

1975 ◽  
Vol 141 (2) ◽  
pp. 306-321 ◽  
Author(s):  
D Mouton ◽  
Y Bouthillier ◽  
N Feingold ◽  
J Feingold ◽  
C Decreusefond ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
Selective Breeding ◽  
Genetic Regulation ◽  
Cumulative Effect ◽  
Phagocytic Index ◽  
Large Individual ◽  
High Responsiveness ◽  
Low Responder ◽  
Glyceryl Trioleate ◽  
Very High

The phagocytic index K, established from the rate of blood clearance of colloidal carbon, measures the phagocytic activity of RE macrophages in contact with the circulating blood. The intravenous injection of glyceryl trioleate (triolein) produces a marked stimulation of the phagocytic activity of RE macrophages. This response is higher in the female than in the male mice. The phenotypic character "responsiveness of macrophage to triolein" presents large individual variants in population of random bred albinos mice. This character is submitted to polygenic regulation. Starting from a foundation population of 25 males and 25 females random bred albinos, mice, two lines were separated by selective breeding for the character "responsiveness to triolein": a "high" responder line, KTH, and a "low" responder line, KTL. After 26 consecutive generations of selective breeding, KTH mice present a very high response to triolein while KTL mice are almost irresponsive. The heritability of this character (h2) calculated from the interline divergence is of 12% plus or minus 1. This value of h2 indicates that the character investigated is determined by the cumulative effect of a group of about 27 independently segregating loci. The distribution of the character in (KTH plus KTL)F1 and their backcrosses with parental lines suggests that low responsiveness is dominant over high responsiveness. The genetic regulation of responsiveness to triolein is independent from the dose administered. These results are discussed in relation to the importance of genetic factors controlling macrophage functions involved in lipid metabolism and in the specific mechanisms of immunity.

Enhanced Phagocytic Activity of Blood Leukocytes in Athymic Nude Mice

1984 ◽  
Vol 35 (6) ◽  
pp. 605-615 ◽  
Author(s):  
M. Holub ◽  
L. Fornůsek ◽  
V. Větvička ◽  
J. Chalupná
Keyword(s):  
Nude Mice ◽  
Phagocytic Activity ◽  
Blood Leukocytes ◽  
Athymic Nude Mice
Sign in / Sign up

Export Citation Format

Share Document