scholarly journals Metabolic Profiling and Antibacterial Assessment of Calotropis procera extract against Streptococcus group B

2020 ◽  
pp. 24-30
Keyword(s):  
Octadecenoic Acid ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Calotropis Procera ◽  
Zone Of Inhibition ◽  
Antibiotic Resistant ◽  
Streptococcus Group ◽  
Group B ◽  
Antimicrobial Metabolites

Calotropis procera is shrub of Asclepiadaceae family and is a source of antimicrobial metabolites against a greater range of bacteria including Streptococcus Group B (SGB). In the present study, different metabolic compounds were extracted from C. procera using 75% methanol. The extract of C. procera was assessed against different bacteria of the SGB family by estimating the zone of inhibition. Moreover, minimal inhibitory concentration (MIC) of different concentrations e.g., 0.001, 0.01, 0.1, 1.0 and 10 mg/mL were determined through zone of inhibition. Gas chromatography mass spectrometry (GC-MS) analysis of plant extract revealed 24 compounds. The most important compounds of the GC-MS array were including undecane, terephthalic acid, Cyclohexane, dimethyl-propane-thiosulfinate, Fluorobenzoic acid Octadecenoic acid and others. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as an antimicrobial compound. The molecular characterization of different bacteria of SGB used in the study revealed the bacteria were consisted of antibiotic resistant genes against.

scholarly journals Isolation and Molecular Characterization of Antibiotic Producing Bacillus licheniformis Strains Isolated from Soil

2020 ◽  
Vol 14 (4) ◽  
pp. 2363-2370
Author(s):  
Anhar Al-Turk ◽  
Nidal Odat ◽  
Muhannad I. Massadeh
Keyword(s):  
Biochemical Characterization ◽  
Octadecenoic Acid ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Arid Area ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Human Pathogens ◽  
Antibiotic Resistant

Currently, there is an increase prevalence of antibiotic-resistant bacteria worldwide. Therefore, the need for characterization of naturally occuring antibiotics with less antibiotic resistance is required. Soil resources contains valuable antibiotic producing microorganisms that increasingly being utilized for the production of suitable antibiotics. Therefore, this study aimed at identifying an antibiotic bacteria with ability of producing antibiotic that is isolated from soil samples collected from Al Zarqa provenance, an arid area in Jordan. Morphological and biochemical characterization of the isolates were carried out and found that all of the isolates belong to Bacillus genus. Further confirmation of the characterization of the bacteria was done by ribosomal RNA and PCR. The results reveal that the isolates represent Basilluslicheniformis. These bacilli were further investigated for antimicrobial activities against 6 ATCC human pathogens viz., S. aureus, S. pneumonia, Salmonella typhi., E. coli, P. mirabels and E. cloacae. Additionally, the results of Gas Chromatography Mass Spectrometry (GCMS) of ethyl acetate extracts for B. licheniformis secondary metabolites showed that they contain two main antimicrobial compounds namely Pyrrolo [1, 2-a] pyrazine-1, 4-dione,hexahydro and Trans-13-octadecenoic acid. The present work maybe suggests that soil isolates from the studied arid area include antibiotic producing strains that can be utilized commercially.

scholarly journals GC-MS Profiling of Anti-bacterial Metabolic Compounds from the Extract of Azadirachta indica

2020 ◽  
pp. 17-23
Keyword(s):  
Plant Extract ◽  
Plant Extracts ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Dioctyl Phthalate ◽  
Gas Chromatography Mass Spectrometry ◽  
Antimicrobial Compounds ◽  
E Coli

Azadirachta indica is a very common plant used very frequently due to its medicinal significance. The antibacterial activities of 0.001, 0.01, 0.1, 1.0 and 10 mg/mL of the plant extract were determined against different pathogenic bacteria. Concentration of 0.01 mg/mL killed the E. coli, E. aerogenes, P. stuartii and 10, 1.0 and 0.1 mg/mL were very effective against the E. cloacae, K. pneumoniae and P. mirabilis and killed them 100% in culture plates. The plant extracts were analyzed for the characterization of the different antimicrobial compounds through gas chromatography-mass spectrometry (GC-MS). An array of antibacterial compounds such as azulene, tetrasiloxane, phthalic acid, cyclopentasiloxane, hexadecanoic acid, spiropentane, dioctyl phthalate were detected in the plant extract through GC-MS. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as antimicrobial compounds.

scholarly journals Characterization of Bioactive Sesquiterpenes, Organic Acids and Their Derivatives from the Leaves of Psidium guajava Linn

2014 ◽  
pp. 456-467
Author(s):  
Okenwa U. Igwe ◽  
T. Abii
Keyword(s):  
Mass Spectrometry ◽  
Methyl Ester ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Psidium Guajava ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Phytochemical Compounds

The isopropanolic extract of the leaves of Psidium guajava Linn was analyzed by Gas chromatography-mass spectrometry (GC-MS). Fourteen different phytochemical compounds have been characterized, including 1, 3, 3-trimethyl-2-oxabicyclo [2.2.2] octane, (2.06%), 4, 11, 11-trimethyl-bicyclo [7.2.0] undec-4-ene (6.26%), (E,E,E)-2, 6, 6, 9-tetramethyl-1, 4, 8-cycloundecatriene (1.05%), eudesma-4(14),11-diene (5.07%), guaia-1(10), 11-diene (4.63%), 3, 7, 11-trimethyl-1,6,10-dodecatriene-3-ol(4.01%), 1,3-dimethyl-8-(1-methylethyl)-tricyclo [4.4.0.0(2,7)] dec-3-ene (1.66%), 1, 1, 4, 7-tetramethyl-decahydro-1H-cycloprop[e] azulen-4-ol (11.00%), hexadecanoic acid methyl ester (1.53%), 4, 4, 8-trimethyltricyclo [6.3.1.0(1,5)] dodecane-2, 9-diol (2.78%), L-(+)-ascorbic acid 2,6-dihexadecanoate (20.43%), 7-octadecenoic acid methyl ester (3.81%), 3, 7, 11, 15-tetramethyl-2-hexadecen-1-ol (2.24%) and 9-octadecenoic acid (33.47%). The presence of these bioactive compounds in the leaves of Psidium guajava Linn could be the reason behind its use for the treatment of diseases and infections in herbal medicine in Nigeria.  

scholarly journals Comparison of the Phytochemical Composition and Antibacterial Activities of the Various Extracts from Leaves and Twigs of Illicium verum

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3909
Author(s):  
Emirlyn-Cheng Yang ◽  
Ya-Yun Hsieh ◽  
Li-Yeh Chuang
Keyword(s):  
Biological Activities ◽  
Membrane Integrity ◽  
Solvent Polarity ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Supercritical Co2 Extraction ◽  
Antibiotic Resistant ◽  
Illicium Verum ◽  
Phytochemical Components

Previous studies have revealed the numerous biological activities of the fruits of Illicium verum; however, the activities of its leaves and twigs have remained undiscovered. The study aimed to investigate the phytochemical components and antibacterial activity of the various extracts from the leaves and twigs of Illicium verum. The herbal extracts were prepared by supercritical CO2 extraction (SFE) and 95% ethanol extraction, followed by partition extraction based on solvent polarity. Analysis of antimicrobial activity was conducted through the usage of nine clinical antibiotic- resistant isolates, including Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii. Among the tested samples, the SFE extracts exhibited broader and stronger antibacterial activities against the test strains, with a range of MIC between 0.1–4.0 mg/mL and MBC between 0.2–4.5 mg/mL. Observations made through scanning electron microscopy revealed potential mechanism of the antimicrobial activities involved disruption of membrane integrity of the test pathogens. Evaluation of the chemical composition by gas chromatography-mass spectrometry indicated the presence of anethole, anisyl aldehyde, anisyl acetone and anisyl alcohol within the SFE extracts, demonstrating significant correlations with the antibacterial activities observed. Therefore, the leaves and twigs of Illicium verum hold great potential in being developed as new natural antibacterial agents.

scholarly journals Exploration of Antibacterial Compounds from the Commiphora myrrha Through GC-MS and Their Evaluation Against the Bacteria Isolated from Corvus splendens

2020 ◽  
pp. 31-38
Keyword(s):  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Severe Infection ◽  
Antibacterial Activities ◽  
Luria Bertani ◽  
Gas Chromatography Mass Spectrometry ◽  
Antimicrobial Compound ◽  
Hexadecanoic Acid ◽  
House Crow

The Corvus splendens (Indian house crow) normally fed on the garbage therefore, they have many pathogenic bacteria, which can infect the human and cause severe infection in human irrespective to the gender, age and region. The bacteria samples were isolated from the faeces, blood lever, and intestines of the crows in Luria-Bertani (LB) agar plates. The culture plates were incubated at 37°C until the colonies were appeared. The colonies were identified morphologically and through molecular marker using 16S-rDNA. The Commiphora myrrha plant extract was used to determine the antibacterial activities against the pathogenic bacteria isolated from crows. The plant was crushed with 70% methanol and filtered. The filtrates were dried and dissolved in 100% methanol. Antibacterial activities of plant extracts were determined against the pathogenic bacteria isolated from crows. The results showed that the plant extracts were very effective against the pathogenic bacteria and showed 100% antibacterial activities. The plants extracts were analyzed for the characterization of different antimicrobial compound through gas chromatography mass spectrometry (GC-MS). The undecane, tetrasiloxane, hexadecanoic acid, heptasiloxane, benzocyclohepten and many other compounds. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as an antimicrobial compound.

scholarly journals Evaluation of Antimicrobial Activity Determination and Phytochemical Investigation in Selected Plants

2018 ◽  
Vol 9 (12) ◽  
Author(s):  
Singh Nitu ◽  
Patidar K. C.
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Calotropis Procera ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Cryptostegia Grandiflora ◽  
Research Activities ◽  
Agar Disc

The work was intended to investigate the antibacterial activity of the various medicinal plants viz. Calotropis procera, Jatropha curcas, Cryptostegia grandiflora, Clerodendron inermis and Cassia tora, collected from Indore (Madhya Pradesh, India) region. The aim of the study, with an objective, was to evaluate the antimicrobial potentials and to determine the zone of inhibition of extracts of selected plants on some medically important bacterial strains. The antimicrobial activity was determined in the extracts using agar disc diffusion method. Various plant extracts were tested for the antibacterial activities against Bacillus subtilis, Salmonella typhi, Staphylococcus aureus, Escherichia coli and Corynebacterium diphteriae. Zone of inhibition of extracts were compared with that of standards like streptomycin. The outcomes illustrated that the noteworthy inhibition of the bacterial growth was shown against the tested organisms. The phytochemical analyses of the plants were also carried out. The microbial activity of the selected plant was due to the presence of various secondary metabolites. Hereafter, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.

scholarly journals Characterization of secondary metabolites in kasturi mango (Mangifera casturi) using gas chromatography-mass spectrometry

2021 ◽  
Vol 948 (1) ◽  
pp. 012059
Author(s):  
S Zulfina ◽  
M A N Fathoni ◽  
R Poerwanto ◽  
D D Matra
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Vaccenic Acid ◽  
Octadecenoic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Type Analysis ◽  
Tetradecanoic Acid ◽  
Chromatography Mass Spectrometry

Abstract Indonesia is a high biodiversity country for underutilized fruits. Indonesian underutilized fruits contain several beneficial functional foods that are very useful for human health. This study aimed to analyze the secondary metabolites found in Kasturi mango (Mangifera casturi). Fruit sampling was carried out in Hulu Sungai Selatan and Banjar, South Kalimantan. Five types of Kasturi mango were used, namely Kasturi, Pinari, Palipisan, Cuban, and Rawa-rawa, which included aril and rind. The results of this research showed that there are specific compounds in each type. Analysis of secondary metabolites using gas chromatography-mass spectrometry (GC-MS) in Kasturi aril showed that specific compounds were obtained in the Kasturi type, such as 13-tetradecenal ethyl elaidate, spinacene, and (23S)-ethylcholest-5-en-3.beta.-ol. The Rawa-rawa type contained specific compounds such as tetradecanoic acid and cis-vaccenic acid. Analysis of secondary metabolites with GC-MS in Kasturi rind showed that the Kasturi type contained specific compounds, such as distearil phosphite and 13-octadecenoic acid. The Rawa-rawa type contained specific compounds such as docosane and triacontyl acetate. Based on, characterization of secondary metabolites using GC-MS, Kasturi mango showed the high variation of secondary metabolites among their types.

scholarly journals Characterization and Antibacterial Activity of Silver Nanoparticles Biosynthesized Using Leaves Extract of Artemisia sieberi and Calotropis procera

2021 ◽  
Vol 72 (2) ◽  
pp. 76-82
Author(s):  
Saleh H. Salmen ◽  
Nadiah Matluq Alkammash ◽  
Tahani Awad Alahmadi ◽  
Sulaiman Ali Alharbi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Diffusion Method ◽  
Calotropis Procera ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Transmission Electron ◽  
Average Size

The prevalence of antibiotic-resistant bacteria has increased recently leading to the need for novel, natural antibacterial agents such as plant-synthesized silver nanoparticles. Such synthesis is safe, cheap, rapid, non-toxic and environmentally friendly. In this study, characterization of biosynthesized silver nanoparticles from extracts of A. sieberi and C. procera was carried out using transmission electron microscopy, fourier transform infrared and energy dispersive x-ray analysis. Spherical nanoparticles with an average size was ~10 nm for A. sieberi and ~14 nm for C. procera were synthesised; synthesis was most effective using A. sieberi. Antibacterial activity of silver nanoparticles was carried out using the agar-diffusion method and by determination of the minimum inhibitory concentration. Biosynthesized silver nanoparticles showed antibacterial activity against Staphylococcus aureus, MRSA, Salmonella typhimurium and Escherichia coli, with silver nanoparticles extracts from A. sieberi being the most antibacterial.

scholarly journals Forensic Analysis of Wild Toxic and Edible Amanita Mushrooms by Gas Chromatography- Mass Spectrometry

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Rajinder Singh
Keyword(s):  
Octadecenoic Acid ◽  
Western Himalaya ◽  
Forensic Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Mushroom Poisoning ◽  
Mushroom Species ◽  
The North ◽  
Amanita Pantherina ◽  
Nitrogen Containing Compounds

For many years, the foraging and consumption of wild mushrooms has been practised in different parts of the world. Despite having various health benefits, few mushroom species are known for causing toxicity as well. In forensic casework conditions, samples from mushroom poisoning cases can be found in dried or powdered form. So, it becomes necessary to characterize mushroom species for identification purposes. In the present study, volatile fractions of five wild toxic and edible Amanita mushroom species (Amanita muscaria, Amanita pantherina, Amanita caesarea, Amanita subglobosa and Amanita porphyria) were analyzed so as to identify compounds for the characterization of selected mushroom species. The obtained volatile fractions were broadly classified into various chemical classes: alcohols, aldehydes, acids, esters, nitrogen-containing compounds, ketones and miscellaneous. The following compounds; octadecanoic acid, 9-octadecenoic acid (z)-, ethane, 1-chloro-1-fluoro- were the most abundant. The present approach utilizing GC-MS, intends to obtain a fingerprint of each sample for discrimination purposes. Also, this work is the first study on the forensic analysis of methanol-soluble components of Amanita mushroom species from the North-Western Himalaya, India.

Medicinal Plants: Antibacterial Effects and Chemical Composition of Essential Oil of Foeniculum vulgare

2017 ◽  
Vol 8 (01) ◽  
Author(s):  
Azadeh Foroughi ◽  
Pouya Pournaghi ◽  
Fariba Najafi ◽  
Akram Zangeneh ◽  
Mohammad Mahdi Zangeneh ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Screen Test ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Antibacterial Effects ◽  
Foeniculum Vulgare

Medicinal plants are considered modern resources for producing agents that could act as alternatives to antibiotics in demeanor of antibiotic-resistant bacteria. The aim of the study was to evaluate the chemical composition and antibacterial activities of essential oil of Foeniculum vulgare (FV) against Pseudomonas aeruginosa and Bacillus subtilis. Gas chromatography mass spectrometry was done to specify chemical composion. As a screen test to detect antibacterial properties of the essential oil, agar disk and agar well diffusion methods were employed. Macrobroth tube test was performed to determinate MIC. The results indicated that the most substance found in FV essential oil was Trans-anethole (47.41 %), also the essential oil of FV with 0.007 g/ml concentration has prevented P. aeruginosa and with 0.002 g/ml concentration has prevented B. subtilis from the growth. Thus, the research represents the antibacterial effects of the medical herb on test P. aeruginosa and B. subtilis. We believe that the article provide support to the antibacterial properties of the essential oil. The results indicate the fact that the essential oil from the plant can be useful as medicinal or preservatives composition.

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