scholarly journals Exploration of Antibacterial Compounds from the Commiphora myrrha Through GC-MS and Their Evaluation Against the Bacteria Isolated from Corvus splendens

2020 ◽  
pp. 31-38
Keyword(s):  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Severe Infection ◽  
Antibacterial Activities ◽  
Luria Bertani ◽  
Gas Chromatography Mass Spectrometry ◽  
Antimicrobial Compound ◽  
Hexadecanoic Acid ◽  
House Crow

The Corvus splendens (Indian house crow) normally fed on the garbage therefore, they have many pathogenic bacteria, which can infect the human and cause severe infection in human irrespective to the gender, age and region. The bacteria samples were isolated from the faeces, blood lever, and intestines of the crows in Luria-Bertani (LB) agar plates. The culture plates were incubated at 37°C until the colonies were appeared. The colonies were identified morphologically and through molecular marker using 16S-rDNA. The Commiphora myrrha plant extract was used to determine the antibacterial activities against the pathogenic bacteria isolated from crows. The plant was crushed with 70% methanol and filtered. The filtrates were dried and dissolved in 100% methanol. Antibacterial activities of plant extracts were determined against the pathogenic bacteria isolated from crows. The results showed that the plant extracts were very effective against the pathogenic bacteria and showed 100% antibacterial activities. The plants extracts were analyzed for the characterization of different antimicrobial compound through gas chromatography mass spectrometry (GC-MS). The undecane, tetrasiloxane, hexadecanoic acid, heptasiloxane, benzocyclohepten and many other compounds. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as an antimicrobial compound.

scholarly journals GC-MS Profiling of Anti-bacterial Metabolic Compounds from the Extract of Azadirachta indica

2020 ◽  
pp. 17-23
Keyword(s):  
Plant Extract ◽  
Plant Extracts ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Dioctyl Phthalate ◽  
Gas Chromatography Mass Spectrometry ◽  
Antimicrobial Compounds ◽  
E Coli

Azadirachta indica is a very common plant used very frequently due to its medicinal significance. The antibacterial activities of 0.001, 0.01, 0.1, 1.0 and 10 mg/mL of the plant extract were determined against different pathogenic bacteria. Concentration of 0.01 mg/mL killed the E. coli, E. aerogenes, P. stuartii and 10, 1.0 and 0.1 mg/mL were very effective against the E. cloacae, K. pneumoniae and P. mirabilis and killed them 100% in culture plates. The plant extracts were analyzed for the characterization of the different antimicrobial compounds through gas chromatography-mass spectrometry (GC-MS). An array of antibacterial compounds such as azulene, tetrasiloxane, phthalic acid, cyclopentasiloxane, hexadecanoic acid, spiropentane, dioctyl phthalate were detected in the plant extract through GC-MS. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as antimicrobial compounds.

scholarly journals The Antioxidant and Antibacterial Activities of the Essential Oils of Marrubium vulgare Growing Wild in Eastern Algeria

2013 ◽  
Vol 15 ◽  
pp. 159-166 ◽  
Author(s):  
Abderazak Abadi ◽  
Abdellatif Fahima
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Marrubium Vulgare ◽  
Eastern Algeria

The chemical composition was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The constituents were identified by their mass spectra and Kovats’ indices. Fivety (50) components in the oil of M. vulgare were identified. The results demonstrated that the major components of the essential oil were: 4,8,12,16-Tetramethyl heptadecan-4-olid (16.97%), Germacrene D-4-ol (9.61%), α- pinéne (9.37%), Phytol (4.87%), Dehydro-sabina ketone (4.12%), Piperitone (3.27%), δ-Cadinene (3.13%), 1-Octen-3-ol (2.35%) and Benzaldehyde (2.31%). The antioxidant activity of the oils was assessed by measurement of metal chelating activity, the reductive potential, the free radical scavenging (DPPH) assay. The antioxidant activity was compared with that of synthetic antioxidants: butylated hydroxyanisole (BHT), and the essential oils. The results showed as the Essential oil of M. vulgare was evaluated for its antibacterial activities against Gram-positive and Gram negative pathogenic bacteria: Listeria monocytogene, Pseudomonas aeruginosa, Agrobacterium tumefaciens and Salmonella enterica.

scholarly journals Characterization of chemical and volatile compounds in cocoa beans (Theobroma cacao) from highland and lowland areas of Bantaeng, South Sulawesi

2020 ◽  
pp. 94-103
Author(s):  
Norman Hanif ◽  
Jumriah Langkong ◽  
Adiansyah Syarifuddin
Keyword(s):  
Theobroma Cacao ◽  
Fatty Acid Content ◽  
Ash Content ◽  
Gas Chromatography Mass Spectrometry ◽  
Scientific Journals ◽  
Hexadecanoic Acid ◽  
Cocoa Beans ◽  
South Sulawesi ◽  
Volatile Profiles

Volatile profiles of cocoa from highlands and lowlands have not been reported in scientific journals. Therefore, this study aims to determinethe volatile profiles of cocoa beans from highland and lowland regions. Cocoa beans were extracted using Maceration method and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results of this study indicate that the highest free fatty acid content (FFA) of 7.18% was obtained in sample from the highland with 3 days of fermentation (A1B1), the highest water content (4.29%) was obtained incocoa beans sample from lowland with 5 days of fermentation (A1B2), the highest ash content (4.28%) was obtained in cocoa beans sample from lowland with 3 days of fermentation (A2B1), the highest protein content (13.32%)was obtained in sample from the highland with 5 days of fermentation (A1B2), and the highest fat content(49.85%)was obtained incocoa beans sample from lowland with 3 days offermentation. Another differentiating characteristic between cocoa beans from lowland and highland was the occurrenceofn-Hexadecanoic acid and 3,7-dimethyl-3,7-dihydro-1h-purine-2,6-dione- in samples from lowland.

Characterization of Konjac Glucomannan Film Containing the Extracts of Atractylodes lancea and Saussurea lappa

2012 ◽  
Vol 506 ◽  
pp. 401-404
Author(s):  
T. Kaewsomboon ◽  
Komgrit Sawangkan ◽  
Chutimon Satirapipathkul
Keyword(s):  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Mean Value ◽  
Konjac Glucomannan ◽  
Saussurea Lappa ◽  
Atractylodes Lancea ◽  
The Mean

The antibacterial properties of konjac glucomannan (KGM) film containing two herb extracts (Atractylodes lancea and Saussurea lappa) at various concentrations was studied in this present work. This activity was tested against two pathogenic bacteria, Bacillus subtilis and Staphylococcus aureus. The results showed that the mean value of inhibition zone diameter of incorporated films with Saussurea lappa extracts were higher than those incorporating Atractylodes lancea extracts for all concentrations. The film incorporated with S. lappa extract had higher antibacterial activity against both bacteria than incorporated with A. lamcea. For all these levels, it can be seen that the KGM film had satisfactory physical properties and antibacterial activities.

scholarly journals Characterization of Bioactive Sesquiterpenes, Organic Acids and Their Derivatives from the Leaves of Psidium guajava Linn

2014 ◽  
pp. 456-467
Author(s):  
Okenwa U. Igwe ◽  
T. Abii
Keyword(s):  
Mass Spectrometry ◽  
Methyl Ester ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Psidium Guajava ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Phytochemical Compounds

The isopropanolic extract of the leaves of Psidium guajava Linn was analyzed by Gas chromatography-mass spectrometry (GC-MS). Fourteen different phytochemical compounds have been characterized, including 1, 3, 3-trimethyl-2-oxabicyclo [2.2.2] octane, (2.06%), 4, 11, 11-trimethyl-bicyclo [7.2.0] undec-4-ene (6.26%), (E,E,E)-2, 6, 6, 9-tetramethyl-1, 4, 8-cycloundecatriene (1.05%), eudesma-4(14),11-diene (5.07%), guaia-1(10), 11-diene (4.63%), 3, 7, 11-trimethyl-1,6,10-dodecatriene-3-ol(4.01%), 1,3-dimethyl-8-(1-methylethyl)-tricyclo [4.4.0.0(2,7)] dec-3-ene (1.66%), 1, 1, 4, 7-tetramethyl-decahydro-1H-cycloprop[e] azulen-4-ol (11.00%), hexadecanoic acid methyl ester (1.53%), 4, 4, 8-trimethyltricyclo [6.3.1.0(1,5)] dodecane-2, 9-diol (2.78%), L-(+)-ascorbic acid 2,6-dihexadecanoate (20.43%), 7-octadecenoic acid methyl ester (3.81%), 3, 7, 11, 15-tetramethyl-2-hexadecen-1-ol (2.24%) and 9-octadecenoic acid (33.47%). The presence of these bioactive compounds in the leaves of Psidium guajava Linn could be the reason behind its use for the treatment of diseases and infections in herbal medicine in Nigeria.  

Characterization of Antibacterial Effects of Novel Silver Nanoparticles: A Case Study on Pseudomonas as a Model for Gram-Negative Bacteria

2012 ◽  
Vol 621 ◽  
pp. 83-86
Author(s):  
Ting Ting He ◽  
Ya Zhou Zhou ◽  
Juan Yang ◽  
Hai Feng Shi
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Salt Content ◽  
Liquid System ◽  
Luria Bertani ◽  
Ag Nps ◽  
Antimicrobial Effects ◽  
Solid Agar ◽  
High Salt Content

The antimicrobial effects of silver nanoparticles (Ag-NPs) are well known, but Ag-NPs are known to aggregate in medium of high salt content and lose their antibacterial activity. Graphene-based silver nanoparticles (Ag NPs-GE) materials can form stable dispersion in the aqueous solution. This study explores the antimicrobial effects of Ag NPs-GE in pathogenic bacteria, Pseudomonas aeruginosa. The antimicrobial activity of Ag NPs-GE was investigated in Luria-Bertani (LB) medium on solid agar plates and liquid system supplement with various concentrations of Ag NPs-GE. The Ag NPs-GE were shown to be an effective bactericide.

scholarly journals In Vitro Antibacterial Activities of Various Ethanolic Medicinal Plant Extracts Against Some Human Pathogenic Bacteria

2020 ◽  
Vol 8 (6) ◽  
pp. 1272-1276
Author(s):  
Samir K. Ali ◽  
Ghorbat S. Ali ◽  
Berivan Abdulrahman Abdullah
Keyword(s):  
Medicinal Plant ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Nigella Sativa ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Foeniculum Vulgare ◽  
Medicinal Plant Extracts

The widespread use of antibiotics often causes increase in the bacterial drugs resistance and causes many side effects in humans. Medical plants have antimicrobial effects against most pathogenic bacteria and can serve as harmless replacement to antibiotics. The aim of this study was to assess the antibacterial effect of five medicinal plant prevailed in Kurdistan region namely; Cinnamon (Cinnamomum cassia (L.) D.Don), Nigella (Nigella sativa L.), Allium (Allium hirtifolium Boiss.), Carrot (Daucus carota L.), and Fennel (Foeniculum vulgare Mill.) against pathogenic Gram positive bacteria (Staphylococcus aureus), and pathogenic Gram negative bacteria (Salmonella typhi and Escherichia coli). The evaluation of antibacterial activity for these plant extracts was carried out using agar-well diffusion method. Results showed that minimum inhibitory concentration (MIC) of A. hirtifolium against tested bacterial isolates were (25 mg/mL), the lowest MIC values for S. aureus were (25 mg/mL) observed with C. cassia, N. sativa and F. vulgare ethanolic extracts and the lowest MIC of D. carota against bacterial isolates were (50 mg/mL), Also, it was observed that S. aureus was more sensitive than S. typhi and E. coli to plant extracts. The ethanol plant extracts had potential antibacterial activities. However, further studies are required to identify the active compounds which could be used for the preparation of new antimicrobial agents and control the bacterial infectious diseases.

scholarly journals Metabolic Profiling and Antibacterial Assessment of Calotropis procera extract against Streptococcus group B

2020 ◽  
pp. 24-30
Keyword(s):  
Octadecenoic Acid ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Calotropis Procera ◽  
Zone Of Inhibition ◽  
Antibiotic Resistant ◽  
Streptococcus Group ◽  
Group B ◽  
Antimicrobial Metabolites

Calotropis procera is shrub of Asclepiadaceae family and is a source of antimicrobial metabolites against a greater range of bacteria including Streptococcus Group B (SGB). In the present study, different metabolic compounds were extracted from C. procera using 75% methanol. The extract of C. procera was assessed against different bacteria of the SGB family by estimating the zone of inhibition. Moreover, minimal inhibitory concentration (MIC) of different concentrations e.g., 0.001, 0.01, 0.1, 1.0 and 10 mg/mL were determined through zone of inhibition. Gas chromatography mass spectrometry (GC-MS) analysis of plant extract revealed 24 compounds. The most important compounds of the GC-MS array were including undecane, terephthalic acid, Cyclohexane, dimethyl-propane-thiosulfinate, Fluorobenzoic acid Octadecenoic acid and others. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as an antimicrobial compound. The molecular characterization of different bacteria of SGB used in the study revealed the bacteria were consisted of antibiotic resistant genes against.

scholarly journals Synthesis and Characterization of Chemical Compounds Derived From Benzohydrazide and Evaluation of Their Antibacterial Activities

2021 ◽  
Vol 8 (1) ◽  
pp. 5-10
Author(s):  
Maryam Alizadeh ◽  
Ashraf Kariminik ◽  
Ali Akbari
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Choline Chloride ◽  
Antibacterial Activities ◽  
Simple Method ◽  
Major Health Problem ◽  
Ft Ir

Background: The antimicrobial resistance of pathogenic bacteria has emerged as a major health problem in recent years. Extensive research has been conducted to find new antimicrobial agents. Objectives: The aim of this study was to examine the antibacterial activities of benzohydrazide derivatives. Methods: Manganese hydrogen sulfate choline chloride was applied in a simple method for synthesizing benzohydrazide derivatives. Antibacterial activities of the derivatives were assessed against Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Bacillus subtilis, diphtheroids, Salmonella enterica, Serratia marcescens, Pseudomonas aeruginosa, and Klebsiella pneumoniae. The structure of the synthesized compounds was determined employing 1 H/13C NMR and Fourier-transform infrared (FT-IR) spectroscopy. The reactions were carried out in choline chloride dissolved in water at room temperature. Results: The results of this study showed that benzohydrazide derivatives had very desired antibacterial activities against the assessed bacteria. Conclusions: Further investigations are required to assess the safety and efficacy of benzohydrazide derivatives as antibacterial agents in vivo and in vitro.

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