scholarly journals UJI EFEKTIVITAS ANTI BAKTERI EKSTRAK DAUN LEUNCA (SOLANUM NIGRUM) TERHADAP DAYA HAMBAT BAKTERI ESCHERICHIA COLI

2020 ◽  
Vol 3 (1) ◽  
pp. 11-16
Author(s):  
Firdaus Fahdi ◽  
Herviani Sari ◽  
Lisbet Saulina Lubis
Keyword(s):  
Escherichia Coli ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Solanum Nigrum ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Disk Diffusion Method ◽  
Inhibitory Power ◽  
Escherichia Coli Bacteria ◽  
Chemical Contents

Antibacterial is a compound that can inhibit the growth of bacteria that can be used for the treatment of infections in humans. One of the bacteria that can cause interference with the system is the Escherichia coli bacteria, where the Escherichia coli bacteria is widely spread around us. The spread of the Escherichia coli bacteria can occur by direct contact (shaking hands, touching). Then passed on by mouth. Leunca leaves have chemical contents including alkaloids, flavonoids, saponins, tanins. The purpose of this study was to determine whether Leunca's leaf extract (Solanum nigrum) has antibacterial effects against the inhibitory power of Escherichia coli bacteria with a concentration of 50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml, 250 mg/ml. The method of this study was the sample of Leunca leaf obtained from West Lumban Rau Village, Habinsaran Subdistrict, Toba Samosir Regency. Sample preparation was done by making thick extract with the maceration method. An anti-bacterial effectiveness test is done by the disk diffusion method. The results showed Leunca's leaf extract containing alkaloid compounds, flavonoids, saponins, tannins. As well as having antibacterial effectiveness against the inhibition of Escherichia coli bacteria. Average inhibition zone area concentration of 50 mg/ml = 6.1 mm, concentration of 100 mg/ml = 7.3, concentration of 150 mg/ml = 8.8 mm, concentration of 200 mg/ml = 10.8 mm, concentration of 250 mg/ml = 12.6 mm. Conclusion: Leunca (Solanum nigrum) Leaf Extract has antibacterial effects against the inhibitory power of Escherichia coli bacteria with a strong category.

scholarly journals Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 42
Author(s):  
Florentin Natalia Melsadalam ◽  
Dewa Gede Katja ◽  
Meiske S. Sangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder  dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri  dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah  estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri  Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

scholarly journals The Effectiveness of Giving Natural Honey as a Bactericide Against the Growth of Escherichia coli Bacteria

2021 ◽  
Vol 11 (3) ◽  
pp. 183-187
Author(s):  
Mulyadi Djojosaputro ◽  
Moskwadina Gultom
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Clear Zone ◽  
Activity Test ◽  
Escherichia Coli Bacteria ◽  
Natural Honey

Honey is one of the natural treatments with antibacterial effects such as hydrogen peroxide, low pH and short water activity that may inhibit bacterial growth. This study aimed to determine the activity of honey as antibacterial to bacteria Escherichia coli. Antibacterial activity test was done in vitro through diffusion method by using disk disc in MHA media then measuring the diameter of a clear zone that inhibition zone form growth of Escherichia coli bacteria and scaled it millimetre unit. This research was done in the laboratory of the Faculty of Medicine Universitas Kristen Indonesia. The concentrations used are 10%, 30%, 50%, 70%, 100% by using sterile equates as honey diluents. The control used was chloramphenicol, the results obtained at a concentration of 10% with a zone of 7.6 mm inhibition of honey had been able to inhibit the growth of Escherichia coli bacteria, and the optimum concentration was 100% with a zone of 24.5 mm. This test showed that natural honey could inhibit bacteria, which conclude that honey might be used as alternative medicine in society. Keywords: Antibacterial effect, Escherichia coli, honey

scholarly journals Comparison of the Effectiveness of Leaf Extracts of Anredera cordifolia, Psidium guajava and Pogostemon cablin on Inhibitory Power Over Escherichia coli Bacteria

Revista Vitae ◽  
2021 ◽  
Vol 28 (3) ◽  
Author(s):  
Budianto Budianto ◽  
Alif Gita Arum sari ◽  
Nurul Inayah ◽  
F. Fatmaningrum ◽  
Anik Suparmi
Keyword(s):  
Escherichia Coli ◽  
Traditional Medicine ◽  
Leaf Extract ◽  
Local Community ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Pogostemon Cablin ◽  
Inhibitory Power ◽  
Escherichia Coli Bacteria

Background: the current research studies why it is effective using Anredera cordifolia, Psidium guajava, and Pogostemon cablin by the local community as a traditional medicine for diarrhea treatment caused by Escherichia colibacteria. Objectives: We compared the inhibitor effectiveness of three leaf extracts against Escherichia coli; we also identified the anti-bacterial substances contained in leaf extracts. Methods: We determined the bacterial test activity using the "agar diffusion" method and the thin layer chromatography (TLC) as qualitative analysis for determining the anti-bacterial substances contained in the extract. Results: The Pogostemon cablin leaf extract contained terpenoids, phenolic, and flavonoids compound as bacterial inhibitors, and the comparison showed that Pogostemon cablin leaf extract had the greatest bacterial inhibition power. Conclusion: The antibiotic substances found in the leaf extracts of Anredera cordifolia, Psidium guajava, and Pogostemon cablin can be used as traditional medicine. The breakthrough was evidenced by the ability to inhibit Escherichia coli bacteria. This research shows that traditional medicine has ancient knowledge used by this paper

scholarly journals ANTIBAKTERI SENYAWA FENOLIK DARI ALANG -ALANG (IMPERATA CYLINDRICA)

2021 ◽  
Vol 3 (2) ◽  
pp. 124-126
Author(s):  
Jhon Patar Sinurat ◽  
Suci Wulandari ◽  
Rinaldo Berutu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Phenolic Compounds ◽  
Screening Test ◽  
Inhibition Zone ◽  
Imperata Cylindrica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Agar Diffusion Method ◽  
Escherichia Coli Bacteria

Phytochemical screening test proved that the extract of Reeds (Imperata cylindrica) contained phenolic compounds tested using 5% FeCl3 reagent. Antibacterial test using agar diffusion method against Reeds extract in DMSO solvent. The phenolic compounds obtained from the saputangan leaves Reeds extract were 36.96 grams after undergoing maceration. Reeds extract of phenolic compounds with concentrations (200; 100; 50 and 25 ppm) had strength antibacterial where the average inhibition zone of Staphylococcus aureus bacteria was 10.0 mm and the average inhibition zone was at Escherichia coli bacteria measuring 10.3 mm.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

Pengaruh Konsentrasi Ekstrak Metanol Daun Sambiloto (Andrographis Paniculata Ness) terhadap Sensitivitas Enterobacteriaceae dengan Metode Difusi

2019 ◽  
Vol 2 (1) ◽  
pp. 26
Author(s):  
Parlindungan Nasution ◽  
Sugito Sugito ◽  
Kuswiyanto Kuswiyanto
Keyword(s):  
Escherichia Coli ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Average Value ◽  
Long Time ◽  
Quasi Experimental ◽  
Bacterial Sensitivity ◽  
Escherichia Coli Bacteria ◽  
Spearman Test

Abstract: Sambiloto is plant one of the natural resources that be come important to used as medicine. Sambiloto effcacy had been known since long time ago. Based on it’s pharmacological properties, Sambiloto leaves provide antidiarrheal activity against bacteria that causing diarehea in humans especially Eacherichia coli and Shigella dysentriae bacteria. The main content of Sambiloto leaves are diterpenoid lactones (andrographolide), paniculides, farnesols and flavonoids. The purpose of this research was to study the effect of the methanol extract concentration of Sambiloto leaves toward the sensitivity of Escherichia coli and Shigella dysentriae bacteria by using diffusion method. Research design using quasi experimental research. Sample used were leaf extract of Sambiloto with concentration of 100%, 75%, 50% and 25% with six times repetition. Data was collected and analyzed by using Spearman analysis. Based on the reseach fnding, the measurement of inhibition zone on Escherichia coli bacteria test found that the average value were 14.67 mm at 100% concentration, 13.67 mm at 75% concentration, 12.17 mm at concentration 50% and 10.00 mm at 25% concentration. While Shigella dysentriae bacteria test obtained that average value were 14.33 mm at 100% concentration, 13.33 mm at 75% concentration, 11.17 at concentration 50% and 9.33 mm at 25% concentration. The data were analyzed by Spearman test and the result was (p = 0,000 <0.01). It showed that there was inhibition zone effect of Sambiloto leaves extract on bacterial sensitivity of Escherichia coli and Shigella dysentriae by using diffusion method. It was concluded that the Sambiloto leaves extract is antibacterial.Abstrak: Tanaman Sambiloto merupakan salah satu bahan alam yang semakin banyak peminatnya untuk dijadikan obat. Khasiat Sambiloto sebenarnya sudah dikenal sejak zaman dahulu. Berdasarkan sifat farmakologinya, daun Sambiloto memberikan aktivitas antidiare terhadap bakteri yang menyebabkan diare pada manusia khususnya bakteri Escherichia coli dan Shigella dysentriae. Kandungan utama dari daun Sambiloto adalah diterpenoide lactones (andrographolide), paniculides, farnesols dan flavonoid. Tujuan dari penelitian ini adalah untuk mengkaji pengaruh konsentrasi ekstrak metanol daun sambiloto terhadap sensitivitas bakteri Escherichia coli dan Shigella dysentriae dengan metode difusi. Desain Penelitian ini berbentuk penelitian eksperimental semu. Sampel yang digunakan adalah ekstrak daun sambiloto dengan konsentrasi 100%, 75%, 50% dan 25% dengan pengulangan sebanyak enam kali. Data dikumpulkandan dianalisis dengan analisis Spearman. Berdasarkan hasil penelitian pengukuran zona hambat pada uji bakteri Escherichia coli didapatkan nilai rata-rata 14.67 mm pada konsentrasi 100%, nilai rata-rata 13.67 mm pada konsentrasi 75%, nilai rata-rata 12.17 mm pada konsentrasi 50% dan nilai rata-rata 10.00 mm pada konsentrasi 25%. Sedangkan uji bakteri Shigella dysentriae didapatkan nilai rata-rata 14.33 mm pada konsentrasi 100%, nilai rata-rata 13.33 mm pada konsentrasi 75%, nilai rata-rata 11.17 pada konsentrasi 50% dan nilai rata-rata 9.33 mm pada konsentrasi 25%. Data dianalisis dengan uji Spearman didapatkan hasil (p = 0,000 < 0.01), menunjukan bahwa terdapat pengaruh zona hambat ekstrak daun sambiloto terhadap sensitivitas bakteri Escherichia coli dan Shigella dysentriae dengan menggunakan metode difusi. Dapat disimpulkan bahawa ekstrak daun sambiloto bersifat antibakteri.

scholarly journals UJI EFEKTIVITAS EKSTRAK BUAH KESEMEK (DYOSPIROS KAKI L.) SEBAGAI ANTIBAKTERI TERHADAP BAKTERI ESCHERICHIA COLI

2019 ◽  
Vol 19 (2) ◽  
pp. 260
Author(s):  
Therry Prosperita K. Wau ◽  
Diaz F. Izdihar ◽  
Katherine Gunawan ◽  
Yolanda Eliza Putri Lubis
Keyword(s):  
Escherichia Coli ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Fruit Extract ◽  
Persimmon Fruit ◽  
Experimental Laboratory ◽  
Escherichia Coli Bacteria ◽  
Mountain Plants

Abstrak : Kesemek (Dyospyros kaki L.) merupakan buah yang berada di tanaman dataran tinggi (pegunungan). Dapat tumbuh dimana saja pada suhu rendah, kelembapan tinggi, intensitas matahari tidak 100% (teduh/mendung). Buah ini mempunyai kandungan senyawa yang memiliki potensi antimikroba yang menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini bertujuan untuk mengetahui adanya efektifitas ekstrak kesemek terhadap pertumbuhan bakteri Escherichia coli pada konsentrasi 100%, 90%, 80%, 70%, 60%, dan 50%. Penelitian ini merupakan penelitian eksperimental laboratotium dengan metode difusi cakram.. Parameter yang diukur adalah besarnya zona hambat minimum dan zona hambat optimum ekstrak terhadap bakteri.  Hasil penelitian didapatkan ekstrak buah kesemek memiliki efektivitas antibakteri pada Escherichia coli, dimanazona hambat minimum rata-ratanya pada konsentrasi 70% (6,1 mm) dan zona hambat optimum rata-rata pada konsentrasi 100% (8,8 mm). Semakin tinggi konsentrasi ekstrak yang diberi maka semakin besar daya antibakterinya. Hasil ini menunjukan bahwa ekstrak buah kesemek memiliki efektivitas antibakteri yang lemah terhadap pertumbuhan bakteri Escherichia coli sesuai klasifikasi respon zona hambat bakteri.Kata kunci: Kesemek (Dyospyros kaki L.), antibakteri, Escherichia coli Abstract : Persimmon (Dyospyros kaki L.) is a fruit that is in highland (mountain) plants. Can grow anywhere at low temperatures, high humidity, the intensity of the sun is not 100% (shade / cloudy). This fruit contains compounds that have antimicrobial potential which inhibits the growth of Escherichia coli bacteria. This study aims to determine the effectiveness of persimmon extract on the growth of Escherichia coli bacteria at concentrations of 100%, 90%, 80%, 70%, 60%, and 50%. This study was an experimental laboratory with disc diffusion method. The parameters measured were the size of the minimum inhibition zone and the optimum zone of extract inhibition against bacteria. The results showed persimmon fruit extracts had antibacterial effectiveness in Escherichia coli, where the minimum inhibition zone was at a concentration of 70% (6.1 mm) and the optimum inhibition zone averaged at a concentration of 100% (8.8 mm). The higher the concentration of extract given, the greater the antibacterial power. These results indicate that persimmon fruit extract has a weak antibacterial effectiveness against the growth of Escherichia coli bacteria according to the classification of bacterial inhibitory zone responses.Keywords: Persimmon (Dyospyros kaki L.), antibacterial, Escherichia coli

scholarly journals Antibacterial activity test of Simargaolgaol (Aglaonema modestum Schott ex Engl) leaves extract against Escherichia coli and Salmonella typhi bacteria

2021 ◽  
Vol 13 (2) ◽  
pp. 151-158
Author(s):  
Tiurma Solomasi Zega ◽  
◽  
Putri Mandaoni Pakpahan ◽  
Rahmayani Siregar ◽  
Givinda Sitompul ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Activity Test ◽  
Inhibitory Power ◽  
Ethanol Extracts

The Simargaolgaol (Aglaonema modestum Schott ex Engl) plant is a plant that grows wild in Barus District, Central Tapanuli, North Sumatra which is used by the community as a medicinal plant. This study aims to determine the antibacterial activity of n-hexane, ethyl acetate and ethanol extracts from Simargaolgaol leaves against Escherichia coli and Salmonella typhi bacteria. The antibacterial activity test of Simargaolgaol leaves was carried out using the disc diffusion method. The results of antibacterial activity showed that the highest inhibitory power of Simargaolgaol leaf extract was ethanol extract (polar), ethyl acetate extract (semi polar) and n-hexane extract (non polar). The inhibitory power of ethanol extract, ethyl acetate, and n-hexane against Escherichia coli bacteria was 13.1 mm (strong); 9.7 mm (medium); 8.0 mm (medium) and for Salmonella typhi bacteria respectively 11.2 mm (strong); 10.7 mm (strong) and 9.3 mm (medium). With the concentration of the extract in the diameter is 10%. Based on this, it can be concluded that Simargaolgaol leaf extract has potential as an antibacterial. Keywords: Aglaonema modestum Schott ex Engl, Antibacterial activity, Escherichia coli, Salmonella typhi

scholarly journals DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

2015 ◽  
Vol 17 (1) ◽  
pp. 149
Author(s):  
Fahri Bian ◽  
Febby E.F. Kandou ◽  
Marhaenus J. Rumondor
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Paper Disk

DAYA HAMBAT EKSTRAK ETANOL Schismatoglottis sp. TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coliABSTRAK Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak etanol daun Schismatoglottis sp. terhadap bakteri Staphylococcus aureus dan Escherichia coli. Daya hambat ekstrak etanol Schismatoglottis sp. terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak etanol Schismatoglottis sp. menghambat pertumbuhan S. aureus pada konsentrasi 60% dan 90% dengan diameter zona hambat 16,10 mm dan 31,33 mm serta  terhadap E. coli pada konsentrasi 30%, 60%, dan 90% dengan diameter zona hambat berturut-turut 11,72 mm, 16,38 mm, dan  29,27 mm. Berdasarkan hasil penelitian, dapat disimpulkan bahwa ekstrak etanol Schismatoglottis sp. terhadap S. aureus pada konsentrasi 60% dan 90% masing-masing memiliki kekautan antibakteri termasuk kategori kuat dan sangat kuat, sedangkan terhadap E. coli pada konsentrasi 30% dan 60%, memiliki kekuatan antibakteri kategoti kuat sedangkan pada konsentrasi 90% memiliki kekuatan antibakteri kategori sangat kuat. Kata kunci : Schismatoglottis sp, Antibakteri, Staphylococcus aureus, Escherichia coli THE INHIBITION OF ETHANOL EXTRACT OF Schismatoglottis sp. AGAINST BACTERIA Staphylococcus aureus AND Escherichia coli. ABSTRACT This study aimed to determine the inhibition of ethanol extract of leaves Schismatoglottis sp. against S. aureus and E. coli. Inhibition of the ethanol extract of Schismatoglottis sp. against to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. Schismatoglottis sp. ethanol extract inhibit the growth of S. aureus at a concentration of 60% and 90% inhibition zone with a diameter of 16.10 mm and 31.33 mm and against E. coli at a concentration of 30%, 60%, and 90% with inhibition zone diameter respectively 11, 72 mm, 16.38 mm and 29.27 mm. Based on the results, it can be concluded that the ethanol extract Schismatoglottis sp. against S. aureus at concentrations of 60% and 90% respectively have antibacterial kekautan categorized as strong and very strong, while against E. coli at a concentration of 30% and 60%, have a strong kategoti antibacterial strength while at a concentration of 90% has antibacterial strength very strong category. Keywords: Schismatoglottis sp, antibacterials, Staphylococcus aureus, Escherichia coli.

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

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