scholarly journals Isolasi, Identifikasi Senyawa Alkaloid Dan Uji Efektivitas Penghambatan Dari Ekstrak Daun Sirih (Piper betle L.) Terhadap Bakteri Staphylococcus epidermidis

2020 ◽  
Vol 8 (2) ◽  
Author(s):  
Gwendolyn L. Kapondo ◽  
. Fatimawali ◽  
Meilani Jayanti
Keyword(s):  
Antibacterial Activity ◽  
Optical Density ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Dilution Method ◽  
Piper Betle ◽  
Betel Leaf ◽  
Before And After ◽  
Density Values

Abstract: Betel leaf (Piper betle L) contains alkaloid compounds and has antibacterial and antiseptic functions. The purpose of this research was to isolated alkaloid compounds also to tested the effectiveness of inhibition of the betel leaf extract against the Staphylococcus epidermidis bacterial growth. Simplicia betel leaf was extracted used 96% of ethanol solvent. Antibacterial activity tested of betel leaf extract with a concentration variation of 25%, 20%, 15%, 10% and 5% using the liquid dilution method. The results of identification of alkaloid isolates used UV-Vis Spectrophotometry method can be known alkaloid compounds contained in betel leaves include alkaloids with indole base framework that was absorption at 262 nm and 274 nm wavelengths. The results of the effectiveness of betel leaf on Bacteria Staphylococcus epidermidis used the liquid dilution method showed that betel leaf extract with Optical density values at concentrations of 25%, 20%, 15% and 10% before and after incubation decreased respectively by -0.347, -0.304, -0.192 and -0.104, while at a concentration of 5% there was increase in Optical density values of 0.162. From the results of the research, it can be concluded that the ethanol extract of betel leaf contains an alkaloid with an indole base framework and was inhibitory activity against Staphylococcus epidermidis bacteria with a MIC at a concentration of 10%.Keywords: betel leaf, Staphylococcus epidermidis, alkaloid compound, liquid dilution.  Abstrak: Daun Sirih (Piper betle L) mengandung senyawa alkaloid dan memiliki fungsi sebagai antibakteri dan antiseptik. Penelitian ini bertujuan untuk mengisolasi senyawa alkaloid dan menguji efektivitas penghambatan dari ekstrak daun sirih hijau terhadap pertumbuhan bakteri Staphylococcus epidermidis. Simplisia daun sirih hijau diekstraksi dengan menggunakan pelarut etanol 96%. Uji aktivitas antibakteri ekstrak daun sirih dengan variasi konsentrasi 25%, 20%, 15%, 10% dan 5% menggunakan metode dilusi cair. Hasil identifikasi terhadap isolat alkaloid menggunakan metode Spektrofotometri UV-Vis dapat diketahui senyawa alkaloid yang terkandung dalam daun sirih termasuk alkaloid dengan kerangka dasar Indol yang mempunyai serapan pada panjang gelombang 262 nm dan 274 nm. Hasil uji efektivitas daun sirih terhadap bakteri Staphylococcus epidermidis dengan metode dilusi cair menunjukan bahwa ekstrak daun sirih dengan nilai densitas optik pada konsentrasi 25%, 20%, 15% dan 10% sebelum dan sesudah inkubasi mengalami penurunan berturut-turut sebesar -0.347, -0.304, -0.192 dan -0.104, sedangkan pada konsentrasi 5% mengalami kenaikan nilai densitas optik sebesar 0.162. Dari hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak etanol daun sirih mengandung alkaloid dengan kerangka dasar indol dan memiliki aktivitas penghambatan terhadap bakteri Staphylococcus epidermidis dengan KHM pada konsentrasi 10%.Kata Kunci: daun sirih, Staphylococcus epidermidis, senyawa alkaloid, dilusi cair.

scholarly journals Antibacterial Activities Test of Combination of Ethanolic Extract of Betel Leaves (Piper betle L.) and Basil Leaves (Ocimum basilicum L.) Against Staphylococcus aureus

2018 ◽  
Vol 9 (2) ◽  
pp. 118-126
Author(s):  
Junie Suriawati ◽  
Patimah Patimah ◽  
Siti Rahayu Rachmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Piper Betle ◽  
Betel Leaf

Betel leaf extract and basil leaf extract contain phenol compounds, safonin, flavonoids, tannins, and essential oils that act as antibacterials. Skin is very susceptible to infection or other skin diseases one of which is caused by Staphylococcus aureus bacteria. This research needs to be done to test the antibacterial activity of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The process of extraction of betel leaf and basil leaves was done with 96% ethanol solvent using maceration method. The combinations used in the sample solution were the concentration of ethanol extract of green betel leaf (0%, 15%, 30%, 45%, 60%, 75%) and basil leaf extract (0%, 15%, 30%, 45%, 60 %, 75%). Furthermore, the antibacterial activity was tested for S. aureus with well diffusion method. The data obtained were tested statistically using Anova and Duncan's advanced test. The result of statistical analysis showed difference of drag zone (p <0,05) between various concentration of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The smallest resin zone of ethanol extract of  betel leaf and ethanol extract of basil leaves is at 0% concentration (S0 and K0) and highest at 75% concentration (S5 and K5). While the smallest zone of inhibition for the combination of ethanol extract of betel leaf and ethanol extract of basil leaves at concentration S0K0, S0K1 and S0K2 is 0 mm and highest at S5K5 concentration is 31,3 mm. The higher concentration of betel leaf extract, basil leaf extract and the combination of the two extracts, the inhibition of diameter zone will be better. It can be concluded that the combination of  betel leaf extract and basil leaf extract has the ability to inhibit the growth of S. aureus better than single extract.

scholarly journals In vitro Study on Anti-salmonella Activities of Boerhaavia diffusa (L. syn) Leaf Extract

2019 ◽  
pp. 1-10
Author(s):  
Ologun, Oluwabusayo ◽  
Dada, Ebenezer Oluyemi ◽  
Ajayi, Kehinde Oluyemi
Keyword(s):  
Antibacterial Activity ◽  
Clinical Isolate ◽  
Optical Density ◽  
Aqueous Extract ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Boerhaavia Diffusa ◽  
Broth Dilution

Various strategies have been employed in the treatment and management of Salmonella infection however, Salmonella strains have gained resistance to antibiotics. This study was to determine in vitro anti-Salmonella activity of Boerhaavia diffusa leaf extract against clinical isolate of Salmonella typhi and Salmonella typhi ATCC 14028. The aqueous and ethanol extracts of B. diffusa were studied for their antibacterial activity against pathogenic Salmonella typhi. This study was carried out between April and September 2018. The in vitro antibacterial activity was performed by agar well diffusion method and broth dilution using spectrophotometric method and the results were expressed as the average diameter of zone of inhibition of bacterial growth around the well and optical density respectively. It was observed that aqueous extract exerted slightly higher activity than ethanolic extract as revealed by the mean diameter of zone of inhibitions at a concentration of 200 mg/ml, the aqueous extract had 35.21±0.47 mm (Salmonella typhi ATCC 14028) compared with ethanol extract 26.41±0.32 mm (clinical). However, in the broth dilution method, ethanol extract significantly (p=0.05) reduced the cell, at 48 hours, the optical density of clinical isolate of S. typhi treated at concentration of 200 mg/ml of extract was 0.47±0.02 nm while at the same concentration of extract, aqueous extract had an optical density of 0.52±0.11 nm respectively. The phytochemical assay revealed that tannin (5.18±0.02 mg/g) and quinone (8.45±0.13 mg/g) in ethanol extract was significantly (p=0.05) higher than aqueous extract while saponin (14.18±0.06 mg/g) was higher in the aqueous extract. The ethanol and aqueous extracts of leaves of B. diffusa whole plant exhibited significant antibacterial activity against both clinical and typed Salmonella typhi. Therefore, the plant extract could be used for the treatment of Salmonellosis, however, the in vivo studies is needed to ascertain the safety of the extract.

Effects of Piper betle Leaf Extract on Biofilm and Rhamnolipid Formation of Pseudomonas aeruginosa

2021 ◽  
pp. 5182-5186
Author(s):  
Irene Ratridewi ◽  
Shod A. Dzulkarnain ◽  
Andreas B. Wijaya ◽  
John T. R. Huwae ◽  
Daniel S. M. Putra ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Linear Regression Analysis ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Piper Betle ◽  
Betel Leaf ◽  
Polystyrene Plate ◽  
Beta Coefficient ◽  
Agar Dilution ◽  
Adherence Test

High mortality rate and antimicrobial resistance are still becoming world-wide problems, due to Pseudomonas aeruginosa’s (P. aeruginosa) virulence and its ability to form biofilm. Biofilm’s formation is affected by the presence of rhamnolipid, whose production is regulated by quorum sensing systems. Piper betle (P. betle) possesses antimicrobial, antioxidant, anti-inflammatory and immunomodulatory properties. The aim of our study is to investigate the effects of P. betle leaf’s extract against biofilm formation and rhamnolipid production of P. aeruginosa. Active compounds of P. betle were identified using plate chromatography. Agar dilution method was used to determine the minimum biofilm inhibitory concentration (MBIC) of methanolic leaf extract of P. betle. A biofilm-producing P. aeruginosa isolate in the polystyrene plate adherence test was selected for confirmation of biofilm production by Scanning Electron Microscopy (SEM), after P. betle administration. Rhamnolipid detection and evaluation were performed by interpreting halo formed around the well. After administration of various concentrations of P. betle leaf extract on the microplate well, it was concluded that the MBIC of P. betle leaf extract on P. aeruginosa was 0.4%. Methanolic extract of P. betle leaf extract at concentration of 0.4% showed that P. aeruginosa could not form biofilm at all, although the bacteria could still aggregate and form a matrix. After linear regression analysis, beta-coefficient was obtained at -0.931 for P. betle leaf extract. It can be concluded that P. betle leaf extract was effective in inhibiting the growth of biofilm and formation of rhamnolipid by P. aeruginosa. The increase in concentration of P. betle leaf extract was inversely proportional to the diameter of the halo rhamnolipid formed. The higher the level of P. betel leaf extract, the smaller the diameter of the halo rhamnolipid formed.

scholarly journals Formulation and Physical Evaluation of Edible Film Dosage from Ethanol Extract of Betel Leaves (Piper betle L) for Canker Sore Drugs

2021 ◽  
Vol 8 (1) ◽  
pp. 42
Author(s):  
Yenni Puspita Tanjung ◽  
Andi Ika Julianti ◽  
Aghnia Wulan Rizkiyani
Keyword(s):  
Leaf Extract ◽  
Corn Starch ◽  
Hydroxypropyl Methylcellulose ◽  
Ethanol Extract ◽  
Edible Film ◽  
Dissolution Time ◽  
Piper Betle ◽  
Betel Leaf ◽  
Physical Evaluation ◽  
Bad Breath

Canker sore which in medical terms is called aphthous stomatitis is a wound in the mouth that can cause pain and discomfort. Piper betel leaf can be used for strengthening the teeth, cure canker sores, treat the bad breath and stop the gum from bleeding. The research aimed to determine the edible film formula of betel leaf ethanol extract that met the physical evaluation requirements and to determine the effect of variation concentration of sorbitol and HPMC (Hydroxypropyl methylcellulose) on the physical evaluation. Edible film preparation from betel leaf extract is a thin layer made from the basic ingredients of corn starch, sorbitol, and HPMC. In this research, 3 edible film formulas were made with variations in the concentration of sorbitol and HPMC, namely: F1 (5%;5%), F2 (4%;4%), F3 (3%;3%). Data of evaluation results were analyzed statistics by Kruskal wallis method. The results showed that F1, F2 and F3 meet the physical evaluation requirements for edible film dosage. Variations in the concentration of sorbitol and HPMC gave different results significantly influence (p<0.05) on weight uniformity, film thickness, solubility and dissolution time, and did not significantly influence (p>0.05) on the organoleptic test, fragility of edible film, water resistance (swelling) and moisture content.Keywords: Betel leaf extract, edible film, HPMC, canker sore, sorbitol 

scholarly journals Uji Antibakteri Sediaan Masker Peel Off Ekstrak Etanol Daun Alpukat (Persea ameicana Mill.) terhadap Bakteri Staphylococcus epidermidis sebagai Antijerawat

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 101
Author(s):  
Esterlina Aldora Puluh ◽  
Hosea Jaya Edy ◽  
Jainer Pasca Siampa
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Persea Americana ◽  
Inhibition Zone Diameter ◽  
Inhibitory Ability ◽  
Activity Test

Daun Alpukat memiliki kandungan antara lain saponin, alkaloid, flavonoid, polifenol, quersetin yang bersifat sebagai antibakteri.  Penelitian ini bertujuan untuk mendapatkan konsentrasi terbaik dari ekstrak etanol daun alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off berdasarkan uji aktivitas antibakteri terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode ekperimental laboratorium. Formula sediaan masker gel peel off ekstrak daun Alpukat dibuat dengan variasi konsentrasi 0.1%; 0.15%; 0.2%; 0.25% dan 0.3%. Ekstrak tanaman daun alpukat diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian antibakteri dengan metode sumuran diameter zona hambat yang paling besar yaitu pada konsentrasi 0,3% dengan diameter zona hambat 3 mm ± 0,32 dan kemampuan daya hambat dikategorikan lemah. Dapat disimpulkan bahwa konsentrasi terbaik dari ekstrak etanol daun Alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off yaitu pada konsentrasi 0.3% dan dikategorikan kemampuan daya hambat lemahAvocado leaves contains saponins, alkaloids, flavonoids, polyphenols, quercetin which are as antibacterial properties. This study aims to obtain the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) which is formulated as a gel peel off mask preparation based on antibacterial activity test against Staphylococcus epidermidis bacteria. This study uses a laboratory experimental method. Avocado leaf extract peel off mask gel formula is made with a concentration variation of 0.1%; 0.15%; 0.2%; 0.25% and 0.3%. Avocado leaf extract is obtained by maceration using 96% ethanol solvent. Antibacterial testing with the largest inhibition zone diameter wells method is at a concentration of 0.3% with a diameter of inhibition zone 3 mm ± 0.32 and the ability of inhibition is categorized as weak. It can be concluded that the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) Which is formulated as a gel peel off mask preparation is at a concentration of 0.3% and categorized as weak inhibitory ability

scholarly journals Antibacterial activity of ethanol extracts of betel leaf (Piper betle L.) and areca (Areca catechu L.) nuts against food borne and oral pathogens

2021 ◽  
Vol 38 (1) ◽  
pp. 15-19
Author(s):  
Masuda Khatun ◽  
Md Mahfuzul Hoque
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Piper Betle ◽  
Areca Nut ◽  
Betel Leaf ◽  
Food Borne ◽  
Areca Catechu ◽  
Oral Pathogens ◽  
Ethanol Extracts

Antimicrobial activity of ethanol extracts of betel leaf (Piper betle L.) and areca nut (Areca catechu) against six food borne enteric pathogens viz. Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (icddr,b), Salmonella typhimurium (AIM-40, icddr,b), Escherichia coli (ATCC 25922), Escherichia coli O157:H7 (ATCC 12079) and Bacillus cereus (ATCC 12079), and four oral pathogens such as isolates 1 & 2 of each of the two bacteria viz. Staphylococcus sp. and Pseudomonas sp. was investigated. Ethanol extract of betel leaf produced highest zone of inhibition (18.0 ± 1.91 mm) against Staphylococcus epidermidis (icddr,b) whereas that of areca nut produced highest zone of inhibition (15.0 ± 0.816 mm) against Staphylococcus aureus (ATCC 25923). But no inhibition was found against Salmonella typhimurium (ATCC AIM-40, icddr,b), Escherichia coli (ATCC 25922), and Escherichia coli O157:H7 (ATCC 12079) by the Ethanol extract of betel leaf. The MIC and MBC values of both ethanol extracts indicated that Gram positive organisms were more susceptible than Gram negative organisms. Highest antimicrobial activity of ethanol extracts of betel leaf was found against the isolate 1 of Staphylococcus sp. (16.5±0.5) and that of areca nut was recorded against the isolate 1 of Staphylococcus sp. (12.0±0.5) and the isolate 2 of Pseudomonas sp. (12.0±0.5). Present study reveals the potentials of both the extracts to inhibit food borne enteric and oral pathogens that could be used as food preservatives to prevent the food borne illness as well as for maintaining the oral and gut health. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 15-19

scholarly journals The Effectiveness Test of Betel Leaf Ethanol Extract Cream (Piper Betle Linn) Toward Propionibacterium acnes Bacterial Growth

2020 ◽  
Vol 4 (2) ◽  
pp. 10-17
Author(s):  
Resva Meinisasti ◽  
Zamharira Muslim ◽  
Krisyanella ◽  
Raden Sunita
Keyword(s):  
Antibacterial Activity ◽  
Active Substance ◽  
Propionibacterium Acnes ◽  
Skin Diseases ◽  
Acne Vulgaris ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Piper Betle ◽  
Betel Leaf ◽  
Acne Treatment

Background: One of skin diseases that is frequently taking attention among teenagers and young adults is acne or in medical term called acne vulgaris. Acne treatment can be treated by repairing follicular abnormalities, decreasing sebum production, decreasing the number of Propionibacterium acnes colonies and reducing inflammation of the skin. The bacterial population of Propionibacterium acnes can be reduced by giving an antibacterial substance such as erythromycin, clindamycin and benzoyl peroxide. In the treatment of acne we can get antibacterial originating from nature, one of which can be obtained in the secondary metabolism of plants. The extracts and essential oils of betel leaf contain antibacterial and antifungal activities. The effectiveness of the use of betel leaf ethanol extract (Piper betle Linn) in acne treatment can be improved by creating formulations in form of cream preparations. Formulations in cream preparations will affect the amount and speed of active substances that can be absorbed. Objectives: To determine the effectiveness of the ethanol extract cream of betel leaf (piper betle linn) cream in formulas with what percentage of active substance has the most-inhibitory effect on the growth of Propionibacterium acnes. Methods:The study used experimental research. Propionibacterium acnes samples were diluted in 0.9% physiological NaCl sterile and embedded in Nutrient Agar (NA) media. The media was inserted into an incubator at 37 ° C for 24 hours. The test of antibacterial activity used the disk diffusion method. The antibacterial activity test results were statistically analyzed using the Statistical Product Services Solution (SPSS 17) program with a confidence level of 95% (α = 0.05). Results: The three creams containing betel leaf ethanol extracts at percentages of 5%, 10% and 15% for each had inhibitory zones: 9.8 mm, 15.85 mm, 17.35 mm. Conclusion: Cream that contains 15% active substance has the strongest inhibition.

scholarly journals Aktivitas antibakteri salep ekstrak etanol daun sirih hijau (Piper betleL.) Terhadap infeksi bakteri Staphylococcus aureus

2018 ◽  
Vol 5 (2) ◽  
pp. 56
Author(s):  
Rissa Laila Vifta ◽  
Muhammad Andri Wansyah ◽  
Anita Kumala Hati
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Piper Betle ◽  
In Vitro Test ◽  
In Vivo Test ◽  
Betel Leaf

<p align="center"><strong>Abstrak </strong></p><p align="center"><strong> </strong></p><p>Sirih hijau (<em>Piper betle L</em>.) adalah tanaman yang mudah dijumpai di Indonesia. Secara empiris, sirih hijau  digunakan sebagai antibakteri pada luka. Penelitian ini bertujuan untuk mengetahui aktivitas salep ekstrak etanol daun sirih hijau sebagai antibakteri. Pengujian efek antibakteri dilakukan secara <em>in vitro </em>dan <em>in vivo</em>. Ekstrak etanol daun sirih hijau dibuat dengan cara maserasi. Pengujian secara <em>in vitro</em> dilakukan menggunakan metode mikrodilusi terhadap <em>Staphylococcus aureus</em>  untuk mengetahui nilai konsentrasi hambat minimum ekstrak daun sirih hijau pada konsentrasi 3, 4, dan 5%.  Kemudian ekstrak dibuat salep dan diuji secara <em>in vivo</em> menggunakan hewan uji tikus yang terdiri dari kelompok kontrol negatif, kelompok ekstrak (konsentrasi 3,4, dan 5%), serata pembanding kontrol positif gentamisin 0,1%. Hasil uji dianalisis menggunakan <em>Sapiro-Wilk</em> dan dilanjutkan dengan uji ANAVA serta uji LSD untuk mengetahui perbandingan hasil kelima kelompok perlakuan. Hasil uji <em>in vitro</em> menunjukkan bahwa konsentrasi hambat minimum ekstrak daun sirih yang optimal adalah 5%. Hasil uji <em>in vivo</em> menunjukkan lama kesembuhan luka meningkat seiring dengan bertambahnya konsentrasi ekstrak daun sirih hijau. Hasil uji normalitas, Anava, dan uji LSD pada uji <em>in vivo</em> memberikan hasil yang sejalan. Konsentrasi 4 dan 5% merupakan konsentrasi efektif dengan aktivitas waktu kesembuhan luka tidak berbeda bermakna dengan kontrol positif (gentamisin 0,1%) dengan rerata waktu berturut-turut 6,20±0,80 dan 6,00±0,71 hari. Dapat disimpulkan bahwa hasil uji antibakteri pada salep sejalan dengan uji secara <em>in-vitro</em> ekstrak daun Sirih Hijau yang memiliki potensi sebagai antibakteri.</p><p><strong>Kata kunci:</strong>     Daun sirih hijau, Antibakteri, Salep, Luka</p><p> </p><p align="center"><strong><em>Antibacterial activity from ointment contains extract of green betle leaves (Piper betle l.) to Staphylococcus aureus bacterial infection</em></strong></p><p align="center"><strong> </strong></p><p align="center"><strong><em>Abstract </em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Green betel (Piper betle L.) is a plant that is easy to find in Indonesia. Empirically, green betel is used as an antibacterial to the wound. This study aims to determine the activity of ointment </em><em>of </em><em>ethanol extract of green </em><em>betel</em><em> leaves as antibacterial. Tests of antibacterial effects were performed in vitro and in vivo. Ethanol extract of green betel leaf is made by maceration. In vitro testing was done using micro dilution method to Staphylococcus aureus to determine the minimum inhibitory concentration of green leaf extract at concentrations of 3, 4, and 5%. Then the extract was made ointment and tested in vivo using rat consisting of the negative control group, the extract group (concentrations 3,4, and 5%), the positive control of gentamicin 0.1%. The test results were analyzed using Sapiro-Wilk and continued with ANAVA test and LSD test to find out the comparison of the results of the five treatment groups. The results of in vitro test showed that the minimum inhibit concentration of </em><em>green </em><em>betel leaf extract was 5%. The results of in vivo test showed the duration of wound healing increased with increasing concentration of green betel leaf extract. The result of normality test, Anava, and LSD test on in vivo test give the same result. Concentrations of 4 and 5% were effective concentrations with wound healing time activity were not significantly different with positive control (gentamicin 0.1%) with mean time of 6.20 ± 0.80 and 6.00 ± 0.71 days respectively. It can be concluded that the antibacterial test results in ointment are in line with in vitro test of leaf </em><em>green betel </em><em>extract which has potential as antibacterial.</em><strong><em></em></strong></p><p><strong><em>Key words</em></strong><em>:      Green betel, Antibacterial, Ointment, Wound</em></p>

scholarly journals Antibacterial Activity of Ethanol Extract of Betel Leaf (Piper betle L.) Against Some Food Borne Pathogens

2012 ◽  
Vol 28 (2) ◽  
pp. 58-63 ◽  
Author(s):  
M Mahfuzul Hoque ◽  
Shemona Rattila ◽  
M Asaduzzaman Shishir ◽  
M L Bari ◽  
Y Inatsu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Foodborne Pathogens ◽  
Ethanol Extract ◽  
Moderate Temperature ◽  
Piper Betle ◽  
E Coli ◽  
Betel Leaf ◽  
Neutral Ph ◽  
And Staphylococcus Aureus

The ethanol extract of Betel leaf (Piper betle L.), an indigenous climber plant of Bangladesh was screened for its antibacterial activity against some foodborne pathogens viz. Vibrio cholerae ATCC 6395, E. coli ATCC 25922,  E. coli O157:H7 NCTC 12049, Shigella dysenteriae-1 MJ-84 and Staphylococcus aureus ATCC 25923. Experimental  findings revealed that the ethanol extract of betel leaves potentially inhibit the growth of these foodborne  pathogens. Minimum inhibitory concentration (MIC) was ranged from 0.625% (w/v) to 0.75% (w/v). The  ethanol extract of betel leaf showed the highest MIC values for E. coli ATCC 25922 (0.625 mm), Vibrio cholera ATCC 6395 (0.625 mm), and Staphylococcus aureus ATCC 25923 (0.625 mm). It was revealed that the antibacterial  activity was highest at around neutral pH and at moderate temperature. The highest temperature (100°C)  and the pH (10.0) treated ethanol extract showed a little less activity than the maximum activity at moderate  temperature and at neutral pH, suggesting that the ethanol extract of the betel leaf was to some extent high  temperature and high pH tolerant. The ethanol extract of betel leaf might have a potential application as  natural food preservative to improve the microbiological safety of the foods. DOI: http://dx.doi.org/10.3329/bjm.v28i2.11817 Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 58-63

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