scholarly journals Induksi Mutasi Kromosom dengan Kolkisin Pada Bawang Putih (Allium sativum L.) Kultivar ‘Kesuna Bali’ (Induced Chromosome Mutation Using Colchicine in Garlic (Allium sativum Linn.) Cultivar ‘Kesuna Bali’)

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Made Pharmawati ◽  
Ni Luh Ayu Jami Wistiani
Keyword(s):  
Chromosome Number ◽  
Traditional Medicine ◽  
Allium Sativum ◽  
Block Design ◽  
Colchicine Treatment ◽  
Horticultural Crop ◽  
Induced Mutation ◽  
Randomized Block Design ◽  
Chromosome Mutation

Abstrak Tanaman bawang putih (Allium sativum L.) adalah tanaman holtikultura yang memiliki banyak manfaat terutama umbinya yang umumnya digunakan sebgai bumbu dan obat. Salah satu kultivar bawang putih yang ditanam di Bali adalah ‘Kesuna Bali’ yang hanya memiliki satu siung.  Salah satu cara untuk memperbaiki karakter tanaman adalah dengan cara induksi mutasi kromosom dengan kolkisin. Penelitian ini bertujuan menganalisis pengaruh perlakuan kolkisin terhadap indeks stomata dan jumlah kromosom dari tanaman ‘Kesuna Bali’. Penelitian menggunakan Rancangan Acak Kelompok dengan enam ulangan. Perlakuan kolkisin yang digunakan adalah 5%, 10% dan 20%. Hasil penelitian menunjukkan bahwa perlakuan kolkisin menurunkan indeks stomata dan meningkatkan jumlah kromosom. Kromosom triploid (2n=3x=24) dihasilkan pada perlakuan kolkisin 20%. Kata kunci : Allium sativum L., ’Kesuna Bali”, kolkisin, mutasi, sitologi Abstract Garlic (Allium sativum L.) is a horticultural crop that has many benefits, especially as spice and traditional medicine.  One of garlic cultivars planted in Bali is ‘Kesuna Bali’ which only has one clove. To improve characters of ‘Kesuna Bali’, modification of ‘Kesuna Bali’ properties can be done by means of induced mutation using colchicine.  This research aims to analyse the effect of colchicine on stomata index and the number of chromosomes of 'Kesuna Bali'. This research used randomized block design with six replicates. In this experiment the concentration of colchicine used were 5%, 10% and 20%. The results of this study showed that colchicine treatment of 20% resulted in the lowest stomata index and there was an increase in chromosome number. Colchicine at concentration of 20% resulted in triploid chromosome set (2n = 3x = 24). Keywords: Allium sativum L., ’Kesuna Bali”, cholchicine, mutation, cytology

scholarly journals Evaluation of Plants Extracts from Capsicum annum and Allium sativum against Aphis craccivora Attacking Cowpea Plant in Kano, Nigeria

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Jibril Abdullahi ◽  
Nuradeen Abdullahi ◽  
Musa Haruna ◽  
Sani Muhammad Yahaya ◽  
Ladan Wada Hayatu ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Allium Sativum ◽  
Concentration Level ◽  
Block Design ◽  
Positive Control ◽  
Aphis Craccivora ◽  
Chemical Insecticide ◽  
Capsicum Annum ◽  
Randomized Block Design ◽  
Safe Food

The efficacy of Capsicum annum fruits and Allium sativum cloves methanolic crude extracts were evaluated on field for the control of Aphis craccivora attacking cowpea plants. Two cowpea genotypes (IAR-48 and IT97K-499-35) were planted separately in a randomized block design, for a cowpea genotype an experimental plots measured 5m x 3m with 1.5m space between plots was demarcated. The plot contained four subplots each measured 1.67m x 3m and replicated three times, given the total of twelve subplots. Within a subplot are three pairs of ridges (70cm apart) tallied with the three different concentrations (200, 600 and 1000ppm) for the plant extracts treatments application. Plant extract treatments along side with synthetic chemical insecticide (Magic force) as positive control were applied to the subplots using Knapsack sprayer 15 days after sowing. The results showed that the population scores of A. craccivora after treatments application on susceptible genotype IAR-48 was reduced significantly (p <0.05) when compared with untreated control subplot. The least population scores of this insect on genotype IAR-48 was recorded in plants treated with A. sativum at 600ppm concentration level which was effective over positive control (synthetic chemical). The genotype IT97K-499-35 recorded no population aphid in plants treated with A. sativum at 200ppm concentration level which is also effective over the positive control. There is critical need to enhance the use of plant extracts scientifically on field as part of Integrated Pests Management for safe food production.

scholarly journals Kombinasi Bawang Putih (Allium sativum), Serai (Cymbopogon citratus) dan Sirsak (Annona muricata) sebagai Pestisida Nabati pada Kutu Daun (Aphis gossypll) Tanaman Cabai Rawit (Capsicum frutescens)

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wahyu Eko Pranoto ◽  
Saimul Laili ◽  
Ratna Djuniwati Lesminingsih
Keyword(s):  
Allium Sativum ◽  
Block Design ◽  
Capsicum Frutescens ◽  
Annona Muricata ◽  
Cymbopogon Citratus ◽  
Good Management ◽  
Randomized Block Design ◽  
Natural Pesticide

Chili plants is one of the leading horticultural commodities, but in increasing the production and quality of chili plants against pest attacks are not accompanied by good management and right, so this causes a decrease in the level of chili production. The aim of this research is to find out the combination reaction of garlic (Allium sativum), lemongrass (cymbopogon citratus) and soursop (Annona muricata) as natural pasticides against aphids (Aphis gossypll) of cayenne pepper (Capsicum frutescens). This research was conducted experimentally by using a Randomized Block Design (RBD) with 5 treatments of various kinds of concentrations namely 0%, 10%, 20%, 30%, and 40%; repeated five times. In this study the mixture combination was higher at a concentration of 40% with a mortality of 43.6%.Keywords:: chili plants, pest Aphids, natural pesticide.  ABSTRAKTanaman cabai merupakan salah satu komoditas hortikultura unggulan namun dalam peningkatan produksi dan kualitas tanaman cabai terhadap serangan hama tidak dibarengi dengan pengelolaan yang baik dan benar, sehingga hal ini menyebabkan menurunnya tingkat produksi tanaman cabai. Penelitian bertujuan megetahui reaksi kombinasi bawang putih (Allium sativum), serai (cymbopogon citratus) dan sirsak (Annona muricata) sebagai pastisida alami terhadap hama kutu daun (Aphis gossypll ) tanaman cabai rawit (Capsicum frutescens).  Penelitian ini dilakukan secara eksperimen dengan menggunakan Rancangan AcakKelompok (RAK) dengan 5 perlakuan macam konsentrasi yaitu 0% , 10% , 20% , 30% , dan 40%; diulang lima kali.  Pada hasil penelitian ini kombinasi campuran lebih tinggi pada konsentrasi 40% dengan mortalitas 43,6 %.Kata kunci: cabai rawit, kutu daun, pestisida alami

scholarly journals THE APPLICATION OF BIOFERTILIZER ON GROWTH AND YIELD OF MELON VARIETIES (Cucumis melo L.)

2021 ◽  
Vol 4 (2) ◽  
pp. 50
Author(s):  
Safwandi Safwandi ◽  
Fadli Hanani ◽  
Muhammad Yusuf N ◽  
Jamidi Jamidi ◽  
Hafifah Hafifah ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
Block Design ◽  
Total Dissolved Solids ◽  
Growth And Yield ◽  
Horticultural Crop ◽  
Dissolved Solids ◽  
Randomized Block Design ◽  
Two Factors ◽  
Cucumis Melo L ◽  
Biological Fertilizer

Melon (Cucumis melo L.) is one of potential horticultural crop in Indonesia. Unfortunately, melon production is currently unable to meet national demand. The objective of this study was to investigate the effect of biofertilizer, varieties and interaction between the dose of biofertilizer and varieties on the growth and yield of melons. The study was conducted in January-April 2021 at Paloh Lada Village, Dewantara District and the Laboratory of Agriculture Faculty, Universitas Malikussaleh. These experiments were arranged in a Factorial Randomized Block Design (RBD) with two factors. The first factor was biofertilizer (P) consisting of 4 levels: P0 (0 ml/L), P1 (5 ml/L), P2 (10 ml/L) and P3 (15 ml/L) and the second factor was melon varieties (V): V1 (F1 Pertiwi Anvi) and V2 (F1 Action 88). There was 8 treatment combinations with 3 replications, resulted in 24 experimental units. The interaction between biofertilizers and varieties gave significant effect on the growth and yield of melon, while the interactions between bio fertilizer did not differ on all variables observed. The application of biological fertilizer 10 ml/L on V1 variety (F1 Pertiwi Anvi) is recommendable. There was an interaction between of biological fertilizers and melon varieties on the total dissolved solids (Brix %).

scholarly journals Chromosome status and yield characteristics of soybean (Glycine max L. (Merr)) in saline soil as affected by induced mutation

2021 ◽  
Vol 6 (2) ◽  
pp. 124
Author(s):  
Agus Pernando Simanjuntak ◽  
Diana Sofia Hanafiah ◽  
Luthfi Aziz Mahmud Siregar
Keyword(s):  
Ploidy Level ◽  
Saline Soil ◽  
Block Design ◽  
Colchicine Treatment ◽  
Salinity Treatment ◽  
Branch Number ◽  
Randomized Block Design ◽  
Salinity Levels ◽  
Chromosome Pattern ◽  
Soybean Plants

To fulfill the demand of soybeans, the expansion of the planting area can be one of the choices through the conversion of marginal land by utilizing suboptimal land, such as saline soil. One of the plant breeding techniques to obtain tolerant plants to salinity is the use of chemical mutations, such as colchicine. The study aimed to reveal the effect of salinity on the characters of soybean plants (mutated with colchicine) and the effect of colchicine on the number of chromosomes and ploidy level. This study was conducted from January to March 2020 and carried out in the plastic house of Faculty of Agriculture, Universitas Sumatera Utara. The experiment was arranged in a Randomized Block Design with salinity levels (0 dS.m-1, 2 dS.m-1, 4 dS.m-1, 6 dS.m-1) as treatments and consisted of ten replications in each treatment. The result showed that the soybean plants were not successfully induced by colchicine at a concentration of 0.04 % with soaking duration of 10 hours, as indicated by the number of chromosomes and analysis of ploidy level. The soybeans that had been induced were planted in saline soil. There were significant effects observed on the productive branch, number of filled pods, and the weight of seeds per plant. Colchicine treatment did not successfully affect the number of chromosomes of soybeans but had an impact on the chromosome pattern. In addition, the salinity treatment of 4 dS.m-1 significantly affected the number of filled pods compared to the treatment of 6 dS.m-1.

scholarly journals Effect of NPK fertilizer and microbial consortium to growth and production of garlic (Allium sativum L.)

2020 ◽  
Vol 20 ◽  
pp. 03010
Author(s):  
Eti Heni Krestini ◽  
Ani Susilawati ◽  
Catur Hermanto
Keyword(s):  
Allium Sativum ◽  
Microbial Consortium ◽  
Block Design ◽  
Negative Control ◽  
Yield Component ◽  
Growth And Yield ◽  
Ecological Fitness ◽  
Randomized Block Design ◽  
Number Of Leaves ◽  
Npk Fertilizer

Garlic productivity in Indonesia is relatively low due to ecological fitness, agronomic performance, and pest losses. For the reason, the research was objected to study the effect of microbial consortium and NPK fertilizer toward the growth and production of garlic. This research was conducted at the experimental field of the Indonesian Vegetable Research Institute situated at 1.300 m asl in Lembang – West Jawa – Indonesia from October 2018 until February 2019. The experiment was arranged in randomized block design, consisted of 6 treatments, and 3 replications. The treatments were: 1) no microbial consortium + no NPK fertilizer (negative control), 2) no microbial consortium + 50% NPK fertilizer, 3). no microbial consortium + 100% NPK recommendation, 4) application of microbial consortium + no NPK, 5) application of microbial consortium + 50% of NPK recommendation, and 6). Application of microbial consortium + 100% of NPK recommendation. The results showed that there was no significant effect of NPK fertilizer and the microbial consortium on the growth and production of garlic. However, the application of microbial consortium + 50% of NPK recommendation performed best on plant height and number of leaves, while application of microbial consortium only resulted in the best pseudostem growth and yield component of garlic.

scholarly journals Herbal Combination Effect on the Quality of Semen and Libido in Ongole Cattle Cross-breed

2017 ◽  
Vol 22 (2) ◽  
pp. 84 ◽  
Author(s):  
Lukman Affandhy ◽  
Dian Ratnawati ◽  
Mochamad Luthfi
Keyword(s):  
Herbal Medicine ◽  
Traditional Medicine ◽  
Semen Quality ◽  
Block Design ◽  
Sperm Concentration ◽  
Herbal Formula ◽  
Traditional Herbal Medicine ◽  
Randomized Block Design ◽  
Sperm Volume

Bulls fertility has an important role to conception, so bulls should have a high libido, high quality semen, and good performance. This study aimed to improve the quality of semen bulls using traditional herbal medicine by utilizing a combination of medicinal plants. The study used 20 heads of Peranakan Ongole (PO) bulls, is divided into four trials traditional herbs, namely Curcuma xhanthorriza, Curcuma eoriginosa, Alpinia galanga, Andrographis paniculata, Piper retrofractum, Eurycom longifolia, Pimpenella purwatjan, each of which is F1 (15%, 15%, 20%, 5%, 30%, 15% and 0%); F2 (15%, 15%, 20%, 5%, 30%, 10% and 5%); F3 (15%, 15%, 20%, 5%, 25%, 20% and 0%); and F4 (15%, 15%, 20%, 5%, 25%, 15% and 0%). Giving the bull 50 g dose of herbal formula every 300 kg of body weight bull mixed with 200 ml honey and 5 eggs. Bulls were given traditional herbal formula once a week orally. The dependent variable of sperm volume, quality of sperm and strength libido were assessed with randomized block design one way. The results showed that all four traditional herbal formulas that are given to PO bulls did not appear to significantly affect the volume of semen and libido for a month with an average volume of 3.8 ± 2.8 cc / ejaculate and libido 159.9 ± 20.2 seconds. Sperm motility, sperm concentration, percentage of live sperm, and sperm abnormalities in PO bulls were given traditional herbal medicine with four different formulas also not significant (P> 0.05); with as well as the color, pH and consistency is still within the normal range. It was concluded that the four herbal formula derived from a mixture of traditional medicine can be given to bulls in order to increase libido and semen quality. It is suggested for granting herbal medicine ingredient formula of traditional medicine that are cheap and easily obtained to bulls.

scholarly journals PENGARUH APLIKASI PUPUK SULFUR DAN JUMLAH SIUNG TERHADAP PERTUMBUHAN DAN PRODUKSI BAWANG PUTIH (Allium sativum L.) SERTA RESIDU SULFUR DI INCEPTISOL KARANGPLOSO

2020 ◽  
Vol 8 (1) ◽  
pp. 43-50
Author(s):  
Balqis Noor Hanifah ◽  
Retno Suntari ◽  
B Baswarsiati
Keyword(s):  
Plant Height ◽  
Allium Sativum ◽  
Growth Parameters ◽  
Block Design ◽  
Randomized Block Design ◽  
Number Of Leaves ◽  
Effect On Growth

Garlic (Allium sativum L.) is a plant that is often used by Indonesian people in various ways. One of the reasons for the low productivity of garlic in Indonesia is the insufficient use of superior varieties and low S elements in the soil. Furthermore, the use of the number of cloves planted per planting hole will determine the number of populations so that it will affect the growth and production of garlic. The aim of this study was to analyze the effect of S fertilizer and the number of cloves on the growth and production of garlic (Allium sativum L.) and also analysis S residue from S fertilizer and number of cloves. This study used a randomized block design with nine treatment combinations, three levels of sulfur fertilizer doses (0 kg S ha-1, 140 kg S ha-1 and 200 kg S ha-1) and the number of cloves (1 clove, 2 cloves and 3 cloves). The results showed that the application of sulfur fertilizer and the number of cloves had no significant effect on growth parameters (plant height and number of leaves) in garlic plants. However, it significantly increased the diameter of bulbs, fresh bulbs weight and dry bulbs weight respectively by 13.39%, 140.72% and 93.64% to 24.29 mm, 5.97 g plant-1 and 4.57 g plant-1 in the treatment of one number of clove and 140 kg of S ha-1 compared to the treatment of one clove and without S. As well as significant effect on SO42- residues and total S with the highest yield respectively 299.3 ppm and 910 ppm and the lowest yield was obtained in the treatment without S fertilizer.

scholarly journals Respon Bawang Putih Tunggal (Allium sativum L.) pada Aplikasi Lama Induksi Siplo dan Urine Kelinci

2020 ◽  
Vol 4 (2) ◽  
pp. 1
Author(s):  
S Sugiarto ◽  
S Sunawan
Keyword(s):  
Cation Exchange ◽  
Allium Sativum ◽  
Block Design ◽  
Biological Properties ◽  
Local Potential ◽  
Nutritional Content ◽  
Novel Technology ◽  
Physical Chemical ◽  
Randomized Block Design ◽  
Two Factors

Onions single (Allium sativum. L) relatively possess better qualities of nutritional content compared to bulbous garlic. Improving the quality and quantity of single  garlic can  be done through the improvement of cultivation. intensification of local potential systems techniques is a novel technology using electricity induction to  improve physical chemical and biological properties  in the soil. The electricity induction applied  into the soil would increase ion and cation exchange  in the ground. The purpose of this study was  Improve the quality and quantity production of single garlic.The research used Randomized block design with two factors. Factors I: Time induction ( I0: 0 minutes, I1: 20, minutes I2: 40, minutes I3: 60 minutes). Factors II: Urine rabbits concentration ( U0: 0 ml /l, U1: 50 ml/l,  U2: 100 ml/l, U3: 150 ml/l.  The result showed a good impact of the application to the production. The application of 60 minutes electricity induction increased the production aroung 51.80 %. Keywords:  single onions, induction siplo, urine

Innovation of Natural Preservation of Mackerel (Rastrelliger kanagurta) Using Coconut Coconut Ashes with Drying Time Variations on Hedonics

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Rika Agustina ◽  
Rita Sunartaty ◽  
Teuku Makmur
Keyword(s):  
Research Design ◽  
Block Design ◽  
Drying Time ◽  
Basic Ingredient ◽  
Randomized Block Design ◽  
Salt Substitute ◽  
Long Time ◽  
Time Variations ◽  
Rastrelliger Kanagurta

Coconut frond ash is one of the wastes from coconut trees which has not been maximally utilized. Coconut frond ash contains MgCl2 and KCl so that it can be used as a salt substitute in the process of preserving fish. In this study coconut frond ash was used as a basic ingredient for making dried mackerel with a long time of drying to storage. The purpose of this study was to determine the effect of drying time on mackerel storage. The research design used was a Randomized Block Design (RCBD) with 2 factors studied. The first factor is the drying time consists of 3 levels, namely P1 = 3 days, P2 = 4 days, P3 = 5 days. The second factor is storage which consists of 3 levels, namely S1 = 30 days, S2 = 60 days, S3 = 90 days. Each treatment was repeated 2 times to obtain 18 experimental units to observed hedonic tests. From the results of the study it can be stated that the treatment has a very significant effect (P≥0.01) on the hedonic test which includes (color, aroma, taste and texture).

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

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