scholarly journals Detection of the presence Aeromonas sp on nile tilapia cultured in floating net cage in lake Tondano

2013 ◽  
Vol 1 (3) ◽  
Author(s):  
Wandalia Tantu ◽  
Reiny A Tumbol ◽  
Sammy N.J Longdong
Keyword(s):  
Nile Tilapia ◽  
Production Test ◽  
Biochemical Tests ◽  
North Sulawesi ◽  
Gram Staining ◽  
Fish Samples ◽  
Oxidase Test ◽  
Net Cage ◽  
H2s Production ◽  
Average Body

The purpose of this study was to detect the presence of bacteria Aeromonas sp in tilapia (Oreochromis niloticus). Nine fish with an average body weight of 150-200 g were used in this study as samples. Sampling was conducted at three different locations which were representative of fish culture sites located on Lake Tondano. The number of sample gathered from each location was and 3 fish which were taken randomly. Fish samples were taken by using a scoop, then the samples were taken alive by placing in an oxygen-filled plastic separately and taken direcly to lab Fish Disease Control and Environmental Center, Tateli, Department of Marine and Fisheries of North Sulawesi province, for examination the presence of bacteria. Isolation of bacteria were conducted by taking samples from gill and kidney. This study was carried out from April - July 2013. Identification of the presence of bacteria was done through a series of observations of colony morphology and gram staining of bacteria, followed by a series of biochemical tests: oxidase test , catalase test , test TSIA , H2S production test , indole test , motility test , citrate test, and test O/F. It could be concluded that 22.22 % of farmed nile tilapia in Lake Tondano were infected with the Aeromonas sp with the following percentage from each site: Paleloan village 7.40% , 7.40% Toulimembet village, and 7.40% Eris village. Keywords: Aeromonas sp, isolation, nile tilapia, floating net cage, Lake Tondano

scholarly journals Identification of Growth-related EST-derived Microsatellite Marker in Nile Tilapia (Oreochromis niloticus)

2020 ◽  
Vol 17 (8) ◽  
pp. 867-879
Author(s):  
Sk Md SaeefUlHoque CHISHTY ◽  
Piyapong CHOTIPUNTU ◽  
Sataporn DIREKBUSARAKOM ◽  
Suwit WUTHISUTHIMETHAVEE
Keyword(s):  
Microsatellite Markers ◽  
Nile Tilapia ◽  
Dna Analysis ◽  
Slow Growth ◽  
Fast Growth ◽  
Growth Performances ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Fish Samples ◽  
Average Body ◽  
Average Body Weight

This study aimed at investigating growth-related, EST-derived microsatellite markers in Nile tilapia (O. niloticus). The link between alleles and genotypes of these makers was particularly examined, as well as the growth performances of the samples of offspring produced from the broodstocks of 5 different fishery stations in Thailand. A total of 25 families of offspring were reared to observe the growth performance for a period of 83 days. Fish samples in this study were divided into two categories according to average body weight: fast (+SD) and slow (-SD) growth. Then, the fins of the fish samples were employed to perform DNA analysis. From the fast and slow growth evaluations, 3 families, namely F1, F24 (from Chumphon) and F11 (from Petchaburi), were chosen for the association analysis. A total of 30 fishes with 5 samples from fast and slow growth of each family were utilized. Nineteen EST-derived microsatellite markers were used to genotype 30 DNA samples of the fast and slow growth fishes. Out of 19 loci, 14 loci (i.e., OMO392, OMO051, OMO097, OMO072, OMO327, OMO277, OMO122, OMO193, OMO198, OMO200, OMO335, OMO374, OMO049, and OMO069) were found polymorphic. Another 4 loci (i.e., OMO059, OMO068, OMO315, and OMO337) were observed as monomorphic. Based on the genotype data, there appeared to indicate a strong, significant relationship between allele and growth of the A3 allele of OMO392 locus in the fast growth group. However, no significant genotypes regarding 19 EST-microsatellite were found to be related with growth. This study suggests that the identified allele A3, which has designated the growth hormone related EST-derived microsatellite primer OMO392, can potentially be used to facilitate marker-assisted selection regarding the fast growth of O. niloticus.

scholarly journals Identification of Bacteria on Seblak Food around University of Jember Based on Microbiological Criteria of Processed Food

BIOEDUKASI ◽  
2020 ◽  
pp. 34
Author(s):  
Rizka Maulidya Cahyani ◽  
Joko Waluyo ◽  
Mochammad Iqbal
Keyword(s):  
Morphological Characteristics ◽  
Hot Water ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Gram Staining ◽  
Microbiological Criteria ◽  
Identification Of Bacteria ◽  
Oxidase Test ◽  
Safe Food

The quality of food that is good in bacteriological, chemical and physical must always be maintained in order to avoid diseases or health problems. Healthy and safe food is an important factor to improve the standard of public health. Seblak is a ready-to-eat Indonesian food which until now has never been carried out research about what kinds of bacteria in it. Seblak is a food made from raw crackers which is then deliberately soaked using hot water to have a chewy texture. This study aims to determine what types of bacteria are contained in seblak, through the process of isolation and identification in the macroscopic, microscopic and biochemical way. This study used 5 samples, which was repeated 5 times for each sample. Bacteria were isolated from the sample using spread plate techniques and observed by growing colonies on the plate. Each different colonies was observed microscopically through gram staining and endospore staining. To strengthen the data, biochemical tests were also carried out, biochemical tests that have been done in this study were the oxidase test, catalase test, and indole test. The results of the study showed that the bacteria that were found from the samples are in the genus of Bacillus sp. because they show the morphological characteristics of the colonies that form concentric circles, meanwhile, microscopic observations show morphological characteristics of cells in the form of gram-negative bacilli and have the endospores.

scholarly journals The Presence of Microbial Air Contaminants in the Operating Theatre at a Teaching Hospital in East Coast Malaysia

2021 ◽  
Vol 9 (1) ◽  
pp. 11-16
Author(s):  
Nor Azyan K.M. Khaidi ◽  
Siti M. Anua ◽  
Nurzafirah Mazlan ◽  
Safaa N. Saud
Keyword(s):  
Nosocomial Infections ◽  
Microbial Contamination ◽  
Surgical Site Infections ◽  
Diffusion Test ◽  
Biochemical Tests ◽  
Air Contaminants ◽  
Operation Theatre ◽  
Gram Staining ◽  
Urease Test ◽  
Oxidase Test

Aim: The aim of this study is to determine the presence of microbial air contaminants in the operating theatre at a teaching hospital. Objective: Airborne microbial level in operation theatre is one of the significant risks in hospital as it can increase the surgical site infection and nosocomial infections. Background: Duo SAS Super 360 Air Sampler was used to collect the airborne samples in triplicate each for nutrient and MacConkey agar at eight operation rooms and two corridors for morning and evening sessions. Sampling was conducted for three months and repeated every two months. Microbiological culture, gram staining and biochemical tests such as catalase test, oxidase test, coagulase test, Triple Sugar Iron Agar test, urease test, citrate test, Sulfide, Indole, Motility test, Methyl Red Voges-Proskauer test, disc diffusion test, and Albert’s stain were performed on the pure isolated culture. Methods: Sampling was conducted for three months and repeated every two months. Microbiological culture, gram staining and biochemical tests such as catalase test, oxidase test, coagulase test, Triple Sugar Iron Agar test, urease test, citrate test, Sulfide, Indole, Motility test, Methyl Red Voges- Proskauer test, disc diffusion test, and Albert’s stain were performed on the pure isolated culture. Bacteria that were present in the operation rooms were Bacillus spp., Micrococcus spp. and Staphylococcus spp. while Pseudomonas aeruginosa and Acinetobacter baumannii were absent. The bacteria identified in the operation theater may also cause surgical site infections and nosocomial infections to the patients, although the microbial contamination in the air of the operation theatre is low. Results: The bacteria identified in the operation theater may also cause surgical site infections and nosocomial infections to the patients, although the microbial contamination in the air of the operation theatre is low. Conclusion: Strengthening surveillance on the hygienic condition of the operation theatre and routine sampling is strongly recommended to control all possible sources and types of infection.

scholarly journals Isolation And Characterization Of Gut Bacterial Species Of Earthworm Lampito Mauritii

2019 ◽  
Vol 6 (2) ◽  
pp. 45-50
Author(s):  
Deepak Kumar Parhi ◽  
Sahana Yasmine S ◽  
Sridhar T ◽  
Deepthi M.P ◽  
Kathireswari P
Keyword(s):  
Ecosystem Engineer ◽  
Bacterial Species ◽  
Keystone Species ◽  
Gut Contents ◽  
Production Test ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Predominant Type ◽  
Bacillus Spp

Lampito mauriti is an Anecic earthworm living in the topsoil and it is geophytophagous in nature.This earthworm is an important soil macro fauna as it has the dual role of an ‘ecosystem engineer’ due to the ability to build burrows as well as ‘keystone species’ in soil food webs because of its function in degradationof organic wastes. The present study investigates the gut of this earthworm to find the most predominant bacterium harbored inside. Gut contents were extracted and streaked on bacteriological media. The predominant type of colony was identified based on size, pigmentation, form, margin and elevation. Thendifferent colonies were isolated and streaked separately to get pure colonies. The bacteria was subjected to several biochemical tests like Motility test, Gram staining, Methyl Red test, Voges-Proskauer test, Indole production test, Carbohydrate Fermentation test, Catalase test and Starch Hydrolysis test in order to identify the species. The bacterial species identification was done using Bergey’s manual. The bacterial isolates such as Micrococcus spp, Veillonella spp, Bacillus spp and Streptococcus spp were identified.

scholarly journals Isolation, Screening and Methionine Production by Bacillus species isolated from Poultry Waste

2018 ◽  
pp. 95-101
Keyword(s):  
Escherichia Coli ◽  
Agar Medium ◽  
Bacillus Species ◽  
Poultry Waste ◽  
Bacillus Sp ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Solid Agar ◽  
Hemolysis Test ◽  
Oxidase Test

The current study was focused on isolating and identifying methionine producing bacteria from poultry waste. The bacteria were isolated from poultry waste and the ones which specifically produced methionine on solid agar medium, using a methionine requiring auxotroph (Escherichia coli) were kept for further analysis. Methionine assay was also carried out after fermentation to quantify methionine produced by each isolate. A total of 10 bacteria were isolated of which 2 were confirmed as active methionine producers after halo growth was observed on the solid agar medium. These 2 isolates which produced higher amount of methionine were ultimately selected for further investigation. The methionine producing isolates which were designated PW1 and PW3 were identified as Bacillus sp. respectively after biochemical tests were carried out. The test carried out include; gram staining, motility test, catalase test, citrate test, hemolysis test, indole test, penicillin sensitivity, oxidase test and crystal colony formation. Where PW3 (Bacillus sp.) produced greater amount of methionine (0.54 mg/l), while PW1 (Bacillus sp.) produced 0.20 mg/l after the completion of the methionine assay. Keywords: Keywords: Methionine production, Bacillus species, poultry waste.

scholarly journals Nile Tilapia Oreochromis niloticus Culture Development Strategy In Floating Net Cage Of Galela Lake, North Halmahera Regency, North Mollucas Province

2019 ◽  
Vol 7 (2) ◽  
pp. 449
Author(s):  
Charlly N. Eteke ◽  
Hengky Sinjal ◽  
Edwin L. A. Ngangi ◽  
Suria Darwisito
Keyword(s):  
Development Strategy ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Swot Analysis ◽  
Seed Quality ◽  
Study Data ◽  
Direct Observations ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Net Cage ◽  
Culture Development

This study aims to examine the development strategy of Nile tilapia (Oreochromis niloticus) culture in Galela Lake, North Halmahera Regency, North Mollucas Province. It is a descriptive study. Data collection used direct observations, interviews, and literature studies, and data analysis applied SWOT analysis, and continued with Quantitative Strategic Planning Matrix (QSPM)).  Results revealed that there were 6 priority strategies taken to develop the culture of the nile tilapia (O. niloticus) as follows: 1. Human resources development; 2. Take advantage of the investment-providing institutions for culture development; 3. Optimize the use of nile tilapia culture in the floating net cage; 4. Broaden the marketing access; 5. Increase the fish seed quality and the product quality; 6. Agreement among the fish farmers to maintain security in the floating net cage localities.Keywords: strategy, Galela Lake, nile tilapia, SWOT, QSPM. ABSTRAKPenelitian ini bertujuan untuk mengkaji strategi pengembangan budi daya ikan nila (Oreochromis niloticus) di Danau Galela Kabupaten Halmahera Utara Provinsi Maluku Utara. Penelitan ini merupakan penelitian deskriptif. Teknik pengembilan data melalui observasi langsung dan wawancara, dan studi literature. Analisis data menggunakan analisis SWOT. Dan dilanjutkan dengan uji OSPM  (Quantitative Strategis Planning Matrix).  Hasil dari analisis data menghasilkan enam strategi prioritas untuk pengembangan budi daya ikan nila (oreochromis niloticus) yaitu 1. Peningkatan pembinaan SDM 2. Memanfaatkan lembaga penyedia modal untuk pengembangan budi daya 3. Optimalisasi lahan budi daya ikan nila di karamba Jaring apung (KJA) 4. Memperluas akses pemasaran 5. Peningkatan kualitas benih ikan nila dan kualitas produk 6. Kesepakatan antar pembudidaya dalam menjaga keamanan di lokasi karamba jaring apung (KJA).Kata Kunci : Strategi, Danau Galela, Ikan Nila, Analisis SWOT, Analisis QSPM

scholarly journals DINAMIKA POPULASI IKAN NILA (Oreochromis niloticus Linnaeus, 1758) DI DANAU PANIAI, PAPUA

2018 ◽  
Vol 23 (3) ◽  
pp. 193
Author(s):  
Samuel Samuel ◽  
Yoga Candra Ditya ◽  
Vipen Adiansyah
Keyword(s):  
Fish Species ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Lake Management ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Average Maximum ◽  
Fish Samples ◽  
Information Research ◽  
Endemic Fish ◽  
Average Size

Introduksi ikan nila atau “serapia” (Oreochromis niloticus) di Danau Paniai bertujuan untuk meningkatkan diversitas hasil tangkapan dan mengurangi tekanan eksploitasi terhadap jenis ikan endemik. Pertumbuhan dan produksi ikan nila yang cepat tanpa diiringi upaya pengelolaan akan mengancam keberlanjutan ikan endemik di danau. Upaya pengelolaan sumberdaya ikan di suatu perairan membutuhkan informasi dinamika populasi. Penelitian dinamika dan pengelolaan populasi ikan nila dilakukan pada bulan Februari sampai Oktober 2016. Contoh ikan dikumpulkan dari hasil tangkapan nelayan dengan menggunakan jaring insang dengan berbagai ukuran mata jaring dan dari 4 (empat) enumerator pada tujuh stasiun pengamatan. Hasil penelitian menunjukkan populasi ikan nila di Danau Paniai didominasi ukuran panjang individu antara 15-25 cm sebanyak 67,24%. Pola pertumbuhan ikan jantan dan betina adalah isometrik, panjang maksimum rata-rata (L) adalah 37,28 cm dan koefisien pertumbuhan (K) adalah 0,50 per tahun. Mortalitas alami (M) dan mortalitas penangkapan (F) masing-masing sebesar 0,99 dan 0,54 per tahun. Tingkat eksploitasi (E) diperoleh nilai 0,35 lebih kecil dari nilai optimum (E=0,5). Ukuran rata-rata ikan nila tertangkap (Lc) adalah 20,55 cm lebih besar dari ukuran pertama matang gonad (Lm) sebesar 14,73 cm. Nilai Lc>Lm mengindikasikan sebagian besar populasi ikan nila di Danau Paniai sempat melakukan pemijahan sehingga pemanfaatan lebih atau sama dengan nilai optimum diharapkan dapat meningkatkan pemanfataan ikan nila di Danau Paniai. Introduction of nile tilapia (Oreochromis niloticus) in Lake Paniai known as “serapia” is aimed for improving the diversity of catches and reducing the exploitation pressure on the endemic fish species. The rapid growth, reproduction and production of nile tilapia without its management efforts is a new threat to the sustainability of the existence and utilization of endemic fish species in the lake. Management efforts of fish resources require population dynamics information. Research on the dynamics and management of nile tilapia populations was conducted from February to October 2016. Fish samples were collected from fishermen catches using nets with various mesh sizes and from four enumerators at seven observation stations. The results showed that the population of nile tilapia in Lake Paniai was dominated by individual length between 15-25 cm with frequency of 67,24%. The growth pattern of male and female fish were isometric, the average maximum length (L) was 37.28 cm and the growth coefficient (K) was 0.50 per year. Natural mortality (M) and fishing mortality (F) were 0.99 and 0.54 per year respectively. Exploitation rate (E) of 0.35 was smaller than the optimum value (E=0.5). The average size of nile tilapia captured (Lc) was 20.55 cm larger than the first size of gonad maturity (Lm) of 14.73 cm. The Lc value was higher than that the Lm value(Lc>Lm) indicating that most of nile tilapia population in Lake Paniai has spawned so that increasing the more or equal to the optimum value was expected to improve the of nile tilapia fish in Lake Paniai.

Bacillus thermotolerans sp. nov., a thermophilic bacterium capable of reducing humus

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3672-3678 ◽  
Author(s):  
Guiqin Yang ◽  
Xuemei Zhou ◽  
Shungui Zhou ◽  
Dehui Yang ◽  
Yueqiang Wang ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Cellular Fatty Acid ◽  
Thermophilic Bacterium ◽  
Anaerobic Growth ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Link Type ◽  
Gram Staining

A novel thermotolerant bacterium, designated SgZ-8T, was isolated from a compost sample. Cells were non-motile, endospore-forming, Gram-staining positive, oxidase-negative and catalase-positive. The isolate was able to grow at 20–65 °C (optimum 50 °C) and pH 6.0–9.0 (optimum 6.5–7.0), and tolerate up to 9.0 % NaCl (w/v) under aerobic conditions. Anaerobic growth occurred with anthraquinone-2,6-disulphonate (AQDS), fumarate and NO3 - as electron acceptors. Phylogenetic analysis based on the16S rRNA and gyrB genes grouped strain SgZ-8T into the genus Bacillus , with the highest similarity to Bacillus badius JCM 12228T (96.2 % for 16S rRNA gene sequence and 83.5 % for gyrB gene sequence) among all recognized species in the genus Bacillus . The G+C content of the genomic DNA was 49.3 mol%. The major isoprenoid quinone was menaquinone 7 (MK-7) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major cellular fatty acid was iso-C16 : 0. On the basis of its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-8T ( = CCTCC AB 2012108T = KACC 16706T) was designated the type strain of a novel species of the genus Bacillus , for which the name Bacillus thermotolerans sp. nov. is proposed.

scholarly journals Microbiological Study of Chronic Suppurative Otitis Media

2019 ◽  
Vol 46 (2) ◽  
pp. 7-9
Author(s):  
Rifat Nousin Shumi ◽  
Abdullah Siddiqe ◽  
Arefa Akter
Keyword(s):  
Otitis Media ◽  
Age Groups ◽  
Chronic Suppurative Otitis Media ◽  
Klebsiella Pneumonia ◽  
Biochemical Tests ◽  
College Hospital ◽  
Suppurative Otitis Media ◽  
Medical College ◽  
Microbiological Profile ◽  
Gram Staining

Chronic suppurative otitis media (CSOM) is a prevailing and notorious infection in developing countries causing serious local damage and threatening complications. The purpose of the present study was determine the microbiological profile of isolates from discharge in CSOM. This study was conducted at out-patient department of ENT at Rajshahi Medical College Hospital, Rajshahi from January 2014 to December 2014. Samples were taken from 185 patients (both male and female) in all age groups suffering from chronic suppurative otitis media. Their Gram staining, culture, and biochemical tests were carried out to identify the organisms. It showed the predominance by staphylococcus aereus (29.13%), followed by Pseudomonas Aeruginosa (22.83%), Streptococcus Pyogen (14.96%), E.Coli (9.44%), Proteus Mirabilis (6.29%), Klebsiella Pneumonia ( 4.72%). Bangladesh Med J. 2017 May; 46 (2): 7-9

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

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