scholarly journals In Vitro Inhibitory Activity of Dayak Onion (Eleutherine bulbosa Mill.) Essential Oil Against Klebsiella pneumoniae

2019 ◽  
Vol 3 (3) ◽  
pp. 146
Author(s):  
Marwan Hermawan ◽  
Amanah Amanah ◽  
Nurbaiti Nurbaiti

Background: The cases of Klebsiella pneumoniae infec on in West Java province is s ll high. Klebsiella pneu- moniae bacterium can produce the Extended Spectrum Beta Lactamase enzyme leading to an bio cs resistant, so it is important to look for natural and inexpensive an bio c alterna ves; one of which is Dayak onion plant (Eleutherine bulbosa Mill). The content found in Dayak onion is believed to inhibit the growth of Klebsiella pneu- moniae bacteria. This study aims to determine the effec veness of Dayak onion essen al oil on the growth of Klebsiella pneumoniae bacteria. Methods: This study was an experimental study with a post-test control group design using the culture of Kleb- siella pneumoniae on the media Muller Hinton Agar in 7 groups. Groups 1 to 5 (K1 to K5) were given sequen ally Dayak Onion essen al oil solu on in 10% DMSO solvent with concentra ons of 50%, 25%, 12.5%, 6.25%, and 3.125%. Group 6 (K6) was posi ve control (+) with 5 μg Levofloxacin, and Group 7 (K7) was Nega ve (-) with 10% DMSO. The treatment results were measured by the diameter of the host zone and the collected data were analyzed using One-way ANOVA with appropriate Post hoc analysis. Results: The results showed that that there are differences in each group to inhibit the growth of bacteria (p <0.005). K1 are higher than K2, K3, K4, K5, and K7 with increasing concentra ons showed greater inhibi on. Levofloxacin (K6) showed the highest inhibitory power. Conclusion: The results showed that that there are differences in each group to inhibit the growth of bacteria (p <0.005). K1 are higher than K2, K3, K4, K5, and K7 with increasing concentra ons showed greater inhibi on. Levofloxacin (K6) showed the highest inhibitory power.

e-GIGI ◽  
2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Juvensius R. Andries ◽  
Paulina N. Gunawan ◽  
Aurelia Supit

Abstrak: Minyak cengkeh berguna sebagai antibakteri alami. Minyak esensial dari cengkeh mempunyai fungsi anestetik dan antimikrobial. Zat yang terkandung dalam cengkeh yang bernama eugenol dapat membunuh bakteri termasuk bakteri yang resisten terhadap antibiotika, salah satunya adalah bakteri Streptococcus mutans. Bakteri ini merupakan mikroorganisme penyebab utama terjadinya karies. Tujuan dari penelitian ini yaitu untuk mengetahui efek antibakteri ekstrak cengkeh terhadap bakteri Streptococcus mutans secara in vitro. Penelitian ini merupakan penelitian eksperimental menggunakan post test only control group design. Penelitian ini menggunakan bahan coba ekstrak cengkeh dengan konsentrasi 40%, 60%, dan 80%, Ciprofloxacin, aquades dengan pengulangan sebanyak lima kali. Data dikumpulkan dan dianalisis dengan one-way ANOVA dan post-hoc uji LSD ( = 0,05). Berdasarkan hasil uji statistik penelitian uji efek antibakteri ekstrak cengkeh terhadap bakteri streptococcus mutans secara in vitro, dapat disimpulkaan bahwa ekstrak cengkeh memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans secara in vitro. Hasil uji lanjut post-hoc uji LSD menunjukan daya hambat ekstrak cengkeh 40%, 60%, 80%, lebih kecil (p<0,05) dalam menghambat Streptococcus mutans secara in vitro dibandingkan Ciprofloxacin. Kata Kunci: Ekstrak cengkeh, Streptococcus mutans.   Abstract: Clove oil is useful as a natural antibacterial agent, essential oil of clove has anesthetic and antimicrobial effect. Substances contained in clove called eugenol can kill bacteria including antibiotic resistant bacteria, one of which is the bacteria Streptococcus mutans. This bacteria is a major cause for caries. The purpose of this study was to mengetahui clove extrack antibacterial effects againts Streptococcus mutans bacteria in vitro. This study is an experimental study using a post test only control group design. This research try using clove extract with a concentration of 40%, 60%, and 80%, Ciprofloxacin, aquades repetition five times. Data collected and analyzed by one-way ANOVA and post-hoc LSD test (α = 0.05). Based on the results of the statistical test to test the effects of anti-bacterial research clove extracts against Streptococcus mutans bacteria in vitro, can disimpulkaan that clove extracts have antibacterial effects in inhibiting the growth of Streptococcus mutans bacteria in vitro.further test result post-hoc LSD test shoved its inhibitory clove extract 40%, 60%, 80% smaller (p<0,05)in hibiting Streptococcus mutans in vitro compared Ciprofloxacin. Keywords: clove extract, Streptococcus mutans


2021 ◽  
Vol 14 (3) ◽  
pp. 1381-1387
Author(s):  
Nining Kurniati

Papaya leaves contain several active substances that are thought to inhibit bacterial adhesion. This study aims to determine the inhibitory power of papaya leaf extraction and papaya leaf infusion to the adhesion of Streptococcus mutans bacteria to neutrophils and differences in inhibition in various concentrations. The method used is an in vitro experimental laboratory with a post-test-only control group design. The sample consisted of several concentrations of papaya leaf extraction and papaya leaf infusion (control, 25%, 50%, 75%, and 100%). The treatment group was added with neutrophil isolates and incubated for 3 hours, then exposed to Streptococcus mutans for 2.5 hours. Knowing the adhesion index can be done by calculating the average number of Streptococcus mutans attached to 100 neutrophils. The results showed successively with rounding in control, 25%, 50%, 75%, 100% papaya leaf infusion, adhesion of 9237, 8929, 7436, 3870, 3162 bacteria to neutrophils occurred. The minimum concentration of 25% papaya leaf extraction and 50% papaya leaf infusion can inhibit the adhesion of Streptococcus mutans to neutrophils. There is a difference in the ability between papaya leaf extraction and papaya leaf infusion in inhibiting the adhesion of Streptococcus mutans to neutrophils as follows: papaya leaf extraction 25% to papaya leaf infusion 25% obtained sig value ≥0.001* with a mean square of -16.38, papaya leaf extraction 50% to Papaya leaf infusion 50% got a sig value of ≥0.001* with a mean square of -42.91, 75% papaya leaf extraction to 75% papaya leaf infusion got a sig value of ≥0.001* with a mean square of -28.85 and 100% papaya leaf extraction to 100% papaya leaf infusion obtained a sig value of ≥0.001* with a mean square of -26.11.


2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Andita Fitriani ◽  
Erni Setiyorini ◽  
Farach Khanifah

Pendahuluan : Bakteri Staphylococcus aureus merupakan bakteri yang paling sering menyebabkan infeksi. Pemberian antibiotik merupakan upaya pengendalian terhadap infeksi yang dapat menyebabkan resisten. Bakteri Staphylococcus aureus telah resisten terhadap antibiotik ampisilin, amoksisilin-asam klavulanat, amoksisilin, penisilin G, sulbenisilin, kloramfenikol dan siprofloksasin sehingga penanganan terhadap infeksi Staphylococcus aureus relatif sulit. Daun Srikaya diketahui mengandung senyawa flavonoid, saponin dan tannin yang memiliki efek antimikroba. Metode Penelitian : Dalam penelitian ini ditentukan Kadar Hambat Minimum (KHM) dengan menggunakan metode dilusi padat.Penelitian ini merupakan penelitian eksperimen analitik dengan post test only control group design. Sampel dalam penelitian ini adalah bakteri Staphylococcus aureus yang merupakan stok kultur milik Balai Besar Laboratorium Kesehatan (BBLK) Surabaya. Variabel independen dalam penelitian ini adalah ekstrak daun srikaya (Annona squamosa L.) dengan konsentrasi 3%, 6%, 12% dan 24%. Variabel dependen dalam penelitian ini adalah bakteri Staphylococcus aureus. Data dianalisis dengan uji one way ANOVA dilanjutkan uji Post Hoc LSD dengan nilai probabilitas (p)<0,05. Hasil Penelitian : Hasil penelitian menunjukkan bahwa terdapat penurunan jumlah koloni bakteri Staphylococcus aureus yang berbanding terbalik dengan peningkatan konsentrasi ekstrak daun srikaya mulai dari konsentrasi 3% hingga 24%.Kesimpulan : Kesimpulan pada penelitian ini yaitu ekstrak daun srikaya mempunyai efek antimikroba terhadap pertumbuhan Staphylococcus aureus dengan KHM terletak pada konsentrasi dua kali lipat dari konsentrasi 24%. Saran : Sebagai referensi bakteri yang dapat menyebabkan infeksi dan dapat menangsninys dengan antimikroba alami yang minimefek samping disbanding dengan BKOKata Kunci: Antimikroba, Ekstrak Daun Srikaya, , Kadar Hambat Minimum (KHM), Staphylococcus aureus


2020 ◽  
Vol 13 (01) ◽  
pp. 26-33
Author(s):  
Gempita Cahya aulia tambunan ◽  
Aparna Dutt ◽  
Sayra Nadhifa ◽  
Firdha Amelia ◽  
Ermi Girsang

There are various potential natural anti-diabetic drugs; one of them is lime peel or Citrus amblycarpa. This study was aimed to explore the anti-diabetic activity and phytochemical content of lime peels. This study was an experimental study that used the post-test only control group design. The lime peels that were collected from the Berastagi fruit market in Medan, North Sumatera were extracted using 70% ethanol by maceration methods. The phytochemical screening identified the presence of phenolic, steroid/triterpenoid, terpenoid, saponin, flavonoid, tannin, and alkaloid. Meanwhile, the anti-diabetic activity of lime peels was evaluate using the α-glucosidase enzyme that was gotten from Saccharomyces cerevisiae by α-glucosidase enzyme inhibition methods. Percent of inhibition was express as Mean ± SD and analyzed by One Way ANOVA, Tukey HSD Post Hoc Test, and followed by linear regression. The result of this study showed that there is a significant difference in percentage inhibition α-glucosidase enzyme in each concentration, and it had an IC50 Value amount of 125.93 ± 9.14 µg/mL. The phytochemical content of the lime peels was flavonoid, phenol, steroid/triterpenoid, and alkaloid. Hence, the lime peel has anti-diabetic activity by inhibition of the α-glucosidase enzyme.


2020 ◽  
Vol 3 (3) ◽  
pp. 47
Author(s):  
Dyah Mustikaturrokhmah ◽  
Erika Diana Risanti

Penyakit  infeksi yang disebabkan oleh bakteri masih menjadi masalah kesehatan di Indonesia. Temulawak (Curcuma xanthorrhiza Roxb.) merupakan bahan alami yang mudah ditemui dan memiliki sifat antibakteri. Staphylococcus epidrmidis dan Salmonella typhosa  adalah bakteri Gram positif dan Gram negatif yang menyebabkan beberapa jenis penyakit.  Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol 70% Temulawak (Curcuma xanthorrhiza Roxb.) terhadap Staphylococcus epidermidis dan  Salmonella thyposa secara In Vitro. Desain penelitian yang digunakan adalah eksperimental laboratorium dengan metode post- test only control group design. Konsentrasi ekstrak temulawak yang digunakan adalah 5%, 15% dan 45%. Penelitian menggunakan Media Muller Hilton yang telah diolesi bakteri dan dibuat sumuran dengan diameter 6 mm sebagai tempat ekstrak temulawak. Kloramfenikol digunakan sebagai kontrol postif dan aquabides sebagai kontrol negatif. Diameter zona hambat diukur menggunakan jangka sorong setelah inkubasi pada suhu 37°C selama 24 jam. Hasil analisis data menggunakan Uji Krusskal-Wallis menunjukkan nilai p<0.005 dan Post-Hoc Mann Whitney dengan nilai p=0.008. Hasil tersebut menunjukkan bahwa ekstrak etanol 70% temulawak (Curcuma xanthorrhiza Roxb.) memiliki aktivitas antibakteri terhadap Staphylococcus epidermidis dan Salmonella thyposa secara In Vitro.


Biomedika ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 65-71
Author(s):  
Chintya Mei Desia Hutasoit ◽  
Yuni Setyaningsih ◽  
Andri Pramono

ABSTRACTTrichophyton rubrum is the most common dermatophytosis etiology. The antifungal agent has several problems such as fungal resistance and therapy side effects. Cacao (Theobroma cocoa L.) bean shells extract contained flavonoids, alkaloids, and saponins which have an antifungal effect. This study aimed to test the in vitro cacao bean shells extract antifungal (especially: Trichophyton rubrum) effectiveness by agar well diffusion method. This was an experimental study with a post-test only control group design. This study used cacao bean shells extract concentration 25%, 50%, 75%, and 100%, ketokonazol as positive control, and aquadest as negative control. The average inhibition diameter of 25%, 50%, 75% and 100% cacao bean shells extract concentration were 10.65 mm, 18 mm, 26.92 mm, and 37.22 mm, respectively, while the ketoconazole inhibition diameter was 51.52 mm. Data were analyzed using the Kruskal-Wallis test and post hoc with Mann-Whitney. The results showed significant differences between each treatment group (p0.05). Cacao bean shells extract had an antifungal effect in inhibiting the growth of Tricophyton rubrum invitro.Keywords: Trichophyton rubrum, Cacao Bean Shells, Antifungal, Agar Well Diffusion Method,  ABSTRAKTrichophyton rubrum adalah penyebab paling umum dermatofitosis. Obat dermatofitosis (antijamur) memiliki beberapa masalah seperti resistensi dan efek samping terapi. Ekstrak cangkang biji kakao (Theobroma cocoa L.) mengandung flavonoid, alkaloid, dan saponin yang memiliki efek antijamur. Tujuan penelitian ini untuk menguji efektivitas antijamur (khususnya Trichophyton rubrum) ekstrak cangkang biji kakao secara in vitro dengan metode difusi agar. Penelitian ini merupakan penelitian eksperimental dengan post-test only control group design. Penelitian ini menggunakan konsentrasi ekstrak cankang biji kakao 25%, 50%, 75%, dan 100%, ketokonazol sebagai kontrol positif, dan aquadest sebagai kontrol negatif. Rata-rata diameter hambat konsentrasi 25%, 50%, 75% dan 100%  secara berturut-turut yaitu: 10,65 mm, 18 mm, 26,92 mm, dan 37,22 mm, sedangkan daya hambat ketoconazole adalah 51,52 mm. Data dianalisis menggunakan uji Kruskal-Wallis dan post hoc dengan Mann-Whitney. Hasil penelitian menunjukkan perbedaan yang signifikan antara masing-masing kelompok perlakuan (p 0,05). Ekstrak cangkang biji kakao memiliki efek antijamur dalam menghambat pertumbuhan Tricophyton rubrum invitro.Kata kunci: Trichophyton rubrum, Anti-jamur, Cangkang Biji Kaka, Metode Difusi Sumuran Agar


2013 ◽  
Vol 2 (1) ◽  
pp. 05 ◽  
Author(s):  
Abdul Razak ◽  
Aziz Djamal ◽  
Gusti Revilla

AbstrakJeruk Nipis (Citrus aurantifolia S.) merupakan salah satu tanaman obat keluarga yang banyak terdapat ditengah masyarkat dan banyak digunakan sebagai ramuan tradisional. Bagian yang sering digunakan adalah air perasannya, dengan salah satu manfaat dapat digunakan untuk menghilangkan jerawat serta penyembuhan luka agar tidak terjadi abses. Jerawat dan abses pada luka merupakan salah satu infeksi yang disebabkan oleh bakteri Staphylococcus aureus.Tujuan Penelitian ini adalah untuk mengetahui daya hambat air perasan buah jeruk nipis (Citrus aurantifolia S.) terhadap pertumbuhan bakteri Staphylococcus aureus secara invitro. Penelitian dilakukan dengan metoda eksperimental laboratorium dengan desain postest only control group design yang dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas.Hasil penelitian menunjukan bahwa air perasan buah jeruk nipis memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus dengan berbagai konsentrasi yaitu 25%, 50%, 75%, dan 100% dan terdapat pengaruh lama kontak terhadap pertumbuhan bakteri dimana bakteri tidak tumbuh seteleh kontak 5 menit pertama dan diikuti menit-menit berikutnya dengan air perasan buah jeruk nipis konsentrasi 100%. Jadi, semakin tinggi konsentrasi air perasan buah jeruk nipis dan semakin lama kontak dengan bakteri Staphylococcus aureus maka daya hambatnya semakin baik.Kata kunci: Uji Daya Hambat, Air Perasan Buah Jeruk Nipis, Staphylococcus aureus.Abstract Lime (Citrus aurantifolia S.) is kind of family’s herbal medicine, most using in the community is widely used as a traditional herb. The most common used part is the lime fruit squeeze with one of the function is used for removing acne and wound healing to prevent the form of abscess. Pimples and abscesses of the wound is one of the infections caused by the bacterium Staphylococcus aureus.The purpose of this study was to determine the inhibition of lime fruit (Citrus aurantifolia S.) squeeze towards the growth of the bacteria Staphylococcus aureus in vitro condition. The study was conducted with laboratory experimental methods to the design of control group design postest only performed at the Laboratory of Microbiology Faculty of Medicine, University of Andalas.The results showed that the lime fruit (Citrus aurantifolia S.) squeeze has the ability to inhibite the bacterial growth of Staphylococcus aureus with various concentrations of 25%, 50%, 75%, and 100% and there is the effect of contact time on the growth of bacteria which the bacteria do not grow after contact the first 5 minutes and the next minute followed by lime fruit squeeze with 100% concentration lime fruit squeeze. Thus, the higher the concentration of lime fruit squeeze and the longer the contact with the bacteria Staphylococcus aureus is the better towards.Keywords:Inhibition test, The Lime Fruit Squeeze, Staphylococcus Aureus.


2017 ◽  
Vol 1 (1) ◽  
pp. 28
Author(s):  
Eviana Budiartanti Sutanto ◽  
Taufiq R Nasihun ◽  
Israhnanto Isradji ◽  
Luciana Budiati Sutanto

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in   K2 (K2  32.59  million/mL,  K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.


2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Arpina Saleha ◽  
Raden Roro Ariessanty Alicia Kusuma Wardhani ◽  
Okviyoandra Akhyar

Penelitian ini bertujuan untuk mengetahui ada atau tidaknya pengaruh media permainan ular tangga senyawa terhadap hasil belajar siswa.Penelitian ini menggunakan nonequivalentcontrolgroupdesign. Populasi dalam penelitian ini adalah seluruh siswa kelas X MIA, sedangkan sampel penelitian yang digunakan yaitu X MIA 2 dan X MIA 3.Pada penelitian ini kedua kelas diberikan perlakuan yang berbeda,kelas X MIA 2 dibelajarkan tanpa menggunakan permainan media ular tangga senyawa, sedangkan kelas X MIA 3 dibelajarkan menggunakan media permainan ular tangga senyawa.Teknik pengumpulan data yang digunakan adalah soal tes pilihan  ganda.  Data  yang diperoleh dianalisis  menggunakan  uji mann whitney u.Data hasil penelitian menunjukkan bahwa nilai rata-rata post-test kelas kontrol lebih rendah daripadanilai rata-rata kelas eksperimen.Sehingga dapat disimpulkan bahwa terdapat pengaruh media permainan ular tangga senyawa pada materi tata nama senyawa terhadap hasil belajar siswa di SMA Negeri 1 AlalakThis study aimed to determine whether or not the influence of snake and compound compound media play on student learning outcomes. This study used nonequivalent control group design. The population in this study were all students of class X MIA, while the research samples used were X MIA 2 and X MIA 3. In this study both classes were given different treatments, class X MIA 2 was taught without using snake ladder compound media games, while classes X MIA 3 was taught using the media game snake ladder compound. Data collection techniques usedwere multiple choice test questions. The data obtained were analyzed using the Mann Whitney test. The data of the research showed that the average value of the control class post-test is lower than the average value of the experimental class.  So  it  can  be  concluded  that  there  were  the influence  of  snake  ladder compound  media  on  compound  nomenclature material  on  student  learning outcomes in SMA Negeri 1 Alalak.


2020 ◽  
Vol 19 (03) ◽  
pp. 126-133
Author(s):  
Siska Toloan Toloan ◽  
Harimat Hendarwan

Masa nifas adalah hal sangat penting untuk diperhatikan guna untuk menurunkan angka kematian ibu dan bayi di Indonesia. Penelitian ini bertujuan untuk Mengetahui Pengaruh Senam Nifas Terhadap Penurunan Tinggi Fundus Uteri Dan Lochea Pada Ibu Pasca Bersalin Yang Mendapatkan Inisiasi Menyusu Dini Dan Mobilisasi Dini Di Praktek Bidan Mandiri Kota Depok Tahun 2019. Penelitian ini menggunakan penelitian kuantitatif yang menggunakan desain penelitian Quasy Exsperimental dengan pendekatan post test only control group design. Populasi yang diambil yaitu 65 ibu pasca bersalin. Sampel diambil dengan metode consecutive sampling besar sampel ditentukan dengan menggunakan rumus Federer setiap variabel bebas terdiri atas 3 kelompok dan setiap kelompok sebanyak 9 sampel sehingga jumlah total sampel 27 ibu pasca bersalin. Pengumpulan data berupa data primer dan uji yang digunakan yaitu uji One Way Anova. Dapat dilihat dari nilai signifikan uji One Way Anova pada penurunan tinggi fundus uteri hari pertama sampai hari kesepuluh dengan nilai signifikan yaitu p-value < 0,05 yang artinya H0 ditolak atau ada perbedaan secara signifikan dan dilihat. Hasil post hoc test tidak terdapat perbedaan signifikan pada kelompok inisiasi menyusu dini, inisiasi menyusu dini dan mobilisasi dini dan kelompok inisiasi menyusu dini, mobilisasi dini dan senam nifas terhadap penurunan tinggi fundus uteri.


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