The protective effect of sulforaphane in rats fed on high cholesterol high fructose diets

2020 ◽  
Vol 21 (3) ◽  
pp. 85-90
Author(s):  
Wael El-Shahat ◽  
Mohamed EL-Adl ◽  
Mohamed Hamed ◽  
Youssef El-Saedy
Keyword(s):  
Lipid Profile ◽  
Antioxidant Status ◽  
Nitrosative Stress ◽  
Histopathological Examination ◽  
Basal Diet ◽  
Control Group ◽  
High Cholesterol ◽  
Glycemic Status ◽  
High Fructose Diet ◽  
High Fructose

Objective: To evaluate the protective role of sulforaphane in rats exposed to high cholesterol and high fructose diet. Design: Randomized experimental study. Animals: Twenty-four male Sprague Dawley rats. Procedures: Rats were allocated in groups of six animals to one of four groups. The first group was kept as a control group in which rats were fed on a basal diet for 15 weeks (Control), while in the second group (Control + SFN) rats were fed on the basal diet for 11 weeks then a sulforaphane (SFN) was given (0.5 mg/kg/day) orally for additional 4 weeks. The third group was the high cholesterol high fructose (HCF) where rats were fed on the basal diet mixed with a solution of cholesterol (1 % ) and fructose (10 %) for 15 weeks, while in the fourth group (HCF + SFN) high cholesterol high fructose diet and sulforaphane rats were fed on the basal diet mixed with a solution of cholesterol (1 %) and fructose (10 %) for 11 weeks then a SFN was given orally (0.5 mg/kg/day) for another 4 weeks. Serum and plasma samples were collected to determine the glycemic status, lipid profile, antioxidant status, oxidative and nitrosative stress markers, and apoptotic marker, alongside liver tissue samples for histopathological examination. Results: Results revealed that sulforaphane alleviated the oxidative damage (decreasing MDA and NO) and improved the antioxidant status (reducing glutathione), and enhanced glycemic status through decreasing plasma glucose concentration and decreasing caspase 9 concentration. Conclusion and clinical relevance: It can be suggested that sulforaphane (SFN) can improve insulin resistance (I.R) and improve serum lipid profile.

scholarly journals Investigation of the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino Wistar rats feeding on high-fructose diet

2019 ◽  
Vol 35 (1) ◽  
Author(s):  
Teka Obsa Feyisa ◽  
Daniel Seifu Melka ◽  
Menakath Menon ◽  
Wajana Lako Labisso ◽  
Mezgebu Legesse Habte
Keyword(s):  
Metabolic Syndrome ◽  
Body Weight ◽  
Uric Acid ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Serum Glucose ◽  
Control Group ◽  
Fasting Serum ◽  
High Fructose Diet ◽  
High Fructose

AbstractCoffee is one of the most commonly consumed beverages in the worldwide and is assumed to have protective effects against metabolic syndrome. The present study was aimed at investigating the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino Wistar rats feeding on high fructose diet. A post-test experimental study was conducted on a total of 30 (9–10 weeks old) male albino Wistar rats. The rats were divided into 6 groups: group I (normal control)-fed on standard chow and plain tap water only; group II (fructose control)-fed on standard chow and 20% of fructose solution; group III–VI (treatment groups)-fed on standard chow, 20% of fructose solution and treated with 71, 142, 213 and 284 mg/kg body weight/day of coffee respectively for six weeks. At the end, body weight, serum glucose, uric acid and lipid profile levels were investigated. Data was entered and cleared by epi-data software version 3.1 and analyzed by one way ANOVA followed by Tukey post hoc multiple comparison tests using SPSS V. 23.00. Statistical significance was considered at p < 0.05. The results showed that body weight, fasting serum glucose and uric acid levels significantly lowered in rats treated with 213 (p = 0.047; 0.049; 0.026) and 284 (p = 0.035; 0.029; 0.010) mg/kg body weight/day of coffee compared to fructose control group. Fasting serum triglycide (TG) and low density lipoprotein (LDL-C) levels showed significant reduction in rats treated with 284 mg/kg body weight/day of coffee as compared to fructose control group (p = 0.031; 0.046) respectively. In conclusion, treating rats with coffee decreased body weight, fasting serum glucose, uric acid, TC, TG and LDL-C, and increased HDL-C in a dose dependent manner in rats feeding on high fructose diet, suggesting that coffee consumption may be helpful in ameliorating metabolic syndrome.

Pro-oxidant effects of a high α-tocopherol dose on kidney antioxidant biomarkers and histopathological aspects

2017 ◽  
Vol 87 (3-4) ◽  
pp. 179-190
Author(s):  
Amel Kanane ◽  
Fayrouz Rouaki ◽  
Mohamed Brahim Errahmani ◽  
Abdenour Laraba ◽  
Hayet Mesbah ◽  
...  
Keyword(s):  
Sunflower Oil ◽  
Antioxidant Status ◽  
Basal Diet ◽  
Inflammatory Cells ◽  
Significant Rise ◽  
Control Group ◽  
Histopathological Changes ◽  
Group 5 ◽  
Oxidant Effect ◽  
Higher Doses

Abstract. The aim of this study is to evaluate the effect of α-tocopherol supplementation at two doses (600 and 1200 mg × kg–1) on kidney antioxidant status and the histopathological changes in Wistar rats after 12 weeks of exposure at different diets. Forty rats has been divided into 4 groups of 10 rats each, the control group received basal diet with 5 % fresh sunflower oil (FSO), the second group: 5 % oxidized sunflower oil (OSO), the third group: 5 % OSO supplemented with 600 mg × kg–1 α-tocopherol and the fourth group: 5 % OSO supplemented with 1200 mg × kg–1 α-tocopherol. In OSO groups, the results showed highly significant increases of LPO (from 31.3 ± 0.9 to 53.8 ± 1.2 nmol of MDA formed/min/mg protein, p < 0.0001) with a significant decrease (p < = 0.001) of the antioxidant enzymatic activities (CAT, SOD, GPX, GR and G6PDH), body weight (339 ± 9 to 290 ± 3 g) and α-tocopherol levels (13.6 ± 0.6 to 6.5 ± 0.4 μg/mg protein). In OSO groups with 600 mg × kg–1 α-tocopherol, an antioxidant effect was found, reflected by a return of the parameters to values similar to those of the control group. However, higher doses of α-tocopherol (1200 mg × kg–1) induced a depletion of antioxidant status, α-tocopherol levels (6.0 ± 0.3 μg/mg protein, p < 0.001) and a very highly significant rise (p < 0.0001) of LPO content (54.86 ± 0.01 nmol of MDA formed/min/mg protein). The kidney tissues also showed changes in glomerular, severe inflammatory cells infiltration, and formation of novel vessels. So, we can conclude that the oxidative stress is attenuated by a moderate administration of 600 mg × kg–1 α-tocopherol, while a pro-oxidant effect occurs at 1200 mg × kg–1 α-tocopherol.

Antidiabetic and Hypolipidemic Effects of Extracts of Gymnema Sylvestre in Streptozotocin Induced Type I Diabetes in Rats

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
Nishtha R. Mahida ◽  
G. . Mandali ◽  
Vijaysinh V. Sindha ◽  
S. K. Raval
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Type I Diabetes ◽  
Histopathological Examination ◽  
Diabetic Control ◽  
Hypolipidemic Activity ◽  
Control Group ◽  
Type I ◽  
Gymnema Sylvestre ◽  
Altered Glucose

Gymnema sylvestre of the family Asclepiadaceae is one of the most important medicinal plants of the central eco-region. It is popularly known as Gurmar, which means “sugar killer”. Extract of leaves is reported to have tannins, gum, flavonoids, proteins and saponins. It has displayed a wide array of pharmacological activities. This study was aimed to investigate the antidiabetic and hypolipidemic effects of Gymnema sylvestre extract in experimentally induced diabetes in rats. Diabetes was produced in adult Wistar rats with single dose of streptozotocin (STZ) @ 60 mg/kg b.wt. intraperitoneally. After the confirmation of diabetes on 7th day (sugar >200 mg/dl), alcoholic and aqueous extracts of G. sylvestre (400 mg/kg) were administered orally to the experimental rats from 8th day and continued for 42 days thereafter. The antidiabetic and hypolipidemic activity was estimated by measuring blood glucose, lipid profile and histopathological examination of various tissues from all the groups. Administration of STZ resulted in a significant (p less than 0.01) increase in blood glucose and lipid profile and histopathological alterations in Diabetic control group as compared to healthy control group. Gymnema treatment demonstrated significant (p less than 0.01) antidiabetic effect indicated by restoration of blood glucose compared to STZ control group. The study concluded that extracts of Gymnema sylvestre improved the altered glucose and lipid profile in diabetic rats, suggesting that the Gymnema Sylvestre extracts exhibit the antidiabetic and hypolipidemic activity.

The analysis of surface saccharide profiles through fluorescein-labelled lectins in a rat pancreatic tissue with established metabolic syndrome model

2018 ◽  
Vol 44 (1) ◽  
pp. 98-104
Author(s):  
Yosun Mater ◽  
Sule Beyhan-Ozdas
Keyword(s):  
Metabolic Syndrome ◽  
Metabolic Diseases ◽  
Pancreatic Tissue ◽  
Control Group ◽  
High Fructose Diet ◽  
Tissue Sections ◽  
Membrane Surfaces ◽  
High Fructose ◽  
A Cell ◽  
And Control

Abstract“Glycans”, which are generally referred as oligosaccharides and polysaccharides, are structures that are present on all cellular surfaces with proteins and lipids being attached to their basic chain structures. Many studies in the field of glycobiology have identified the various and complicated biological roles of these glycans which make them perfect molecules to use in labelling and selecting body cells specifically. This study aims at analyzing the modifications in saccharide units of glycans on a cell membrane surfaces of the pancreatic tissue of rats to which normal and metabolic syndrome (MetS) are established. To this end, a MetS model was created through a high fructose diet in Spraque Dawley breed of rats and the pancreatic tissue sections of the group with MetS and control group animals were evaluated comparatively. The targeted saccharide units were examined with Fluorescent Microscope by using two different Fluorescein (FITC) labelled lectins, namely Maackia amurensis-1 lectin [FITC-(MAL-I)] and the Wheat Germ Agglutinin (FITC-WGA). It was observed that FITC-MAL-1-labelled Galβ4GlcNAc units did not change much due to high- fructose diet. On the other hand, more GlcNAc, Neu5Ac and β-GlcNAc units which are labelled with FITC-WGA lectin increase in numbers in pancreatic sections of high fructose diet, compared to control group. Thus, a rapid and specific labelling method, which can identify surface saccharide sequences specifically, was developed. The method can be used in early diagnosis and/or treatment for metabolic diseases.

Beneficial effects of an intradialytic cycling training program in patients with end-stage kidney disease

2015 ◽  
Vol 40 (6) ◽  
pp. 550-556 ◽  
Author(s):  
Carole Groussard ◽  
Myriam Rouchon-Isnard ◽  
Céline Coutard ◽  
Fanny Romain ◽  
Ludivine Malardé ◽  
...  
Keyword(s):  
Body Composition ◽  
Kidney Disease ◽  
Physical Fitness ◽  
Lipid Profile ◽  
Antioxidant Status ◽  
Density Lipoprotein ◽  
Control Group ◽  
Beneficial Effects ◽  
Cycling Training ◽  
Training Protocol

In chronic kidney disease (CKD), oxidative stress (OS) plays a central role in the development of cardiovascular diseases. This pilot program aimed to determine whether an intradialytic aerobic cycling training protocol, by increasing physical fitness, could reduce OS and improve other CKD-related disorders such as altered body composition and lipid profile. Eighteen hemodialysis patients were randomly assigned to either an intradialytic training (cycling: 30 min, 55%–60% peak power, 3 days/week) group (EX; n = 8) or a control group (CON; n = 10) for 3 months. Body composition (from dual-energy X-ray absorptiometry), physical fitness (peak oxygen uptake and the 6-minute walk test (6MWT)), lipid profile (triglycerides (TG), total cholesterol, high-density lipoprotein, and low-density lipoprotein (LDL)), and pro/antioxidant status (15-F2α-isoprostanes (F2-IsoP) and oxidized LDL in plasma; superoxide dismutase, glutathione peroxidase, and reduced/oxidized glutathione in erythrocytes) were determined at baseline and 3 months later. The intradialytic training protocol did not modify body composition but had significant effects on physical fitness, lipid profile, and pro/antioxidant status. Indeed, at 3 months: (i) performance on the 6MWT was increased in EX (+23.4%, p < 0.001) but did not change in CON, (ii) plasma TG were reduced in EX (–23%, p < 0.03) but were not modified in CON, and (iii) plasma F2-IsoP concentrations were lower in EX than in CON (–35.7%, p = 0.02). In conclusion, our results show that 30 min of intradialytic training, 3 times per week for 3 months, are enough to exert beneficial effects on the most sensitive and reliable marker of lipid peroxidation (IsoP) while improving CKD-associated disorders (lipid profile and physical fitness). Intradialytic aerobic cycling training represents a useful and easy strategy to reduce CKD-associated disorders. These results need to be confirmed with a larger randomized study.

Curcumin ameliorates protein expression changes involved in mitochondrial fatty acids metabolism in heart of mice fed a high-fructose diet

2020 ◽  
Author(s):  
Cecilia Gabriela Meléndez-Salcido ◽  
Katya Vargas-Ortiz ◽  
Oscar Gerardo Silva-Gaona ◽  
María Cristina León-García ◽  
Luz Arcelia Ortega-Hernández ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Beneficial Effect ◽  
Therapeutic Potential ◽  
Cardiometabolic Disease ◽  
Control Group ◽  
Standard Diet ◽  
High Fructose Diet ◽  
High Fructose ◽  
Fatty Acids Metabolism

Abstract Background: It has been proposed that curcumin modulates the gene expression of different signaling pathways, improve the fatty acids metabolism and exerts a potential beneficial effect on cardiometabolic disease, but this has not been thoroughly demonstrated. In the present study, we evaluated the effect of curcumin upon the expression of PPARα, CPT1, MCAD, VLCAD and ACAA2 in heart of mice fed a high-fructose diet (HFD). Methods: Twenty-four mice C57BL/6 were divided into four groups (n=6) and treated for 15 weeks. Control group (C) received standard diet (SD), Curcumin group (C+Cur), Fructose group (F) and Fructose with Curcumin group (F+Cur). The groups were treated with 0.75% w/w curcumin mixed in the SD and 30% w/v fructose in water, respectively. Heart proteins expression were analyzed by Western Blot. Results: Curcumin supplementation increased PPARα and ACAA2 expression and decreased CPT1 and MCAD expression in heart of mice fed a HFD. However, it did not modify the VLCAD expression. Conclusions: Curcumin regulated PPARα, CPT1 and MCAD expression and increased ACAA2 expression; suggesting a therapeutic potential in the prevention of alterations in mitochondrial fatty acids metabolism in heart of mice fed a HFD.

Protective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factor

2017 ◽  
Vol 95 (7) ◽  
pp. 850-860 ◽  
Author(s):  
Waleed A.I. Khallaf ◽  
Basim A.S. Messiha ◽  
Amira M.H. Abo-Youssef ◽  
Nesrine S. El-Sayed
Keyword(s):  
Nitric Oxide ◽  
Cognitive Impairment ◽  
Angiotensin Ii ◽  
Nitrosative Stress ◽  
Histopathological Examination ◽  
Control Group ◽  
Angiotensin Ii Receptor Blocker ◽  
Protective Effects ◽  
Brain Levels ◽  
Tnf Α

Angiotensin II has pro-inflammatory and pro-oxidant potentials. We investigated the possible protective effects of the Angiotensin II receptor blocker telmisartan, compared with the superoxide scavenger tempol, on lipopolysaccharide (LPS)-induced cognitive decline and amyloidogenesis. Briefly, mice were allocated into a normal control group, an LPS control group, a tempol treatment group, and 2 telmisartan treatment groups. A behavioral study was conducted followed by a biochemical study via assessment of brain levels of beta amyloid (Aβ) and brain-derived neurotropic factor (BDNF) as amyloidogenesis and neuroplasticity markers, tumor necrosis factor alpha (TNF-α), nitric oxide end products (NOx), neuronal and inducible nitric oxide synthase (nNOS and iNOS) as inflammatory markers, and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione reduced (GSH), and nitrotyrosine (NT) as oxido-nitrosative stress markers. Finally, histopathological examination of cerebral cortex, hippocampus, and cerebellum sections was performed using routine and special Congo red stains. Tempol and telmisartan improved cognition, decreased brain Aβ deposition and BDNF depletion, decreased TNF-α, NOx, nNOS, iNOS, MDA, and NT brain levels, and increased brain SOD and GSH contents, parallel to confirmatory histopathological evidences. In conclusion, tempol and telmisartan are promising drugs in managing cognitive impairment and amyloidogenesis, at least via upregulation of BDNF with inhibition of neuroinflammation and oxido-nitrosative stress.

scholarly journals Effect of Papaya Seed Extract (Carica papaya Linn.) on Glucose Transporter 4 (GLUT 4) Expression of Skeletal Muscle Tissue in Diabetic Mice Induced by High Fructose Diet

2017 ◽  
Vol 22 (2) ◽  
pp. 131
Author(s):  
Devyani Diah Wulansari ◽  
Achmad Basori ◽  
Suhartati Suhartati
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Seed Extract ◽  
Skeletal Muscle Tissue ◽  
Control Group ◽  
Test Results ◽  
High Fructose Diet ◽  
Glut4 Expression ◽  
High Fructose ◽  
Papaya Seed

Ethnobotany surveys show that papaya seeds are widely used as herbs for the management of some diseases such as abdominal discomfort, pain, malaria, diabetes, obesity, and infection. This research was conducted to analyze the effect of papaya seed extract on GLUT4 expression on skeletal muscle tissue of DM type II model induced by high fructose diet. This study used 24 animals, divided into 4 groups of negative control group, treated with papaya seed extract 100 mg / kgBB, 200 mg / kgBW and 300 mg / kgBW, was adapted for 14 days then induced by fructose solution 20% Orally with a dose of 1.86 grams / kgBB for 56 days. The treatment group was given papaya seed extract in accordance with the dose of each group for 14 days. GDP levels was measured using a spectrophotometer. Skeletal muscle tissue is used on the gastrocnemius part. GLUT4 expression was measured through a Immunoreactive Score (IRS) method with immunohistochemical staining using GLUT4 polyclonal antibodies. Comparative test results showed that there were significant differences between groups (p <0.05) in final GDP variables and GLUT4 expression. Pearson correlation test results show that the value p = 0.001, meaning there is a significant relationship between GLUT4 expression with final GDP levels. The result of simple linear regression analysis showed that p = 0,000 (<0,05), meaning that dose of papaya seed extract had a significant influence on GLUT4 expression.

scholarly journals Dietary Cold Pressed Watercress and Coconut Oil Mixture Enhances Growth Performance, Intestinal Microbiota, Antioxidant Status, and Immunity of Growing Rabbits

Animals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 212 ◽  
Author(s):  
Mahmoud Alagawany ◽  
Mohamed Abd El-Hack ◽  
Adham Al-Sagheer ◽  
Mohammed Naiel ◽  
Islam Saadeldin ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Antioxidant Status ◽  
Pathogenic Bacteria ◽  
Coconut Oil ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Control Group ◽  
Feed Conversion ◽  
Live Body Weight ◽  
Cold Pressed

The present study assessed the effect of dietary supplementation with coconut oil (CNO), watercress oil (WCO), and their mixture as promoters of growth, antioxidant status, immunity, and intestinal microbiota in growing rabbits. A total of 120 rabbits were distributed into six groups (20 rabbits/group) receiving a basal diet without supplementation (G1) or diet supplemented with 2 g CNO (G2), 2 g WCO (G3), 0.5 g CNO plus 1.5 g WCO (G4), 1 g CNO plus 1 g WCO (G5), or 1.5 g CNO plus 0.5 g WCO/kg (G6). Live body weight and feed conversion ratio were significantly higher in the G4 and G5 groups than in the other groups. Superoxide dismutase activity and reduced glutathione concentration were significantly improved in the CNO or WCO diets. Supplemental CNO plus WCO at all tested levels produced the best lysozyme and complement 3 activities. Cecal lactobacilli, coliform, Enterobacteriaceae, and Clostridium spp. populations were lower in the group who received the 1 g CNO + 1 g WCO/kg diet than that in the control group. Dietary supplementation of 1 g CNO + 1 g WCO or 0.5 g CNO + 1.5 g WCO/kg had the potential to improve growth, feed utilization, antioxidant status, and immunity, and reduce cecal pathogenic bacteria in rabbits.

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