scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI MAHONI (Swietenia mahagoni) TERHADAP Shigella dysenteriae

2017 ◽  
Vol 1 (1) ◽  
pp. 15-21
Author(s):  
Asiska Permata Dewi ◽  
Annisa Fauzana
Keyword(s):  
Public Health Problem ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Shigella Dysenteriae ◽  
Infectious Intestinal Disease ◽  
Negative Controls ◽  
Swietenia Mahagoni

One of the plants that can be used as traditional medicine is mahogany (Sweitenia mahagoni). The presence of flavonoid compounds in mahogany seeds is suspected as a compound that serves to inhibit bacterial growth. One of the antibacterial effects is to treat diarrhea diseases caused by Shigella dysenteriae. Diarrhea is infectious intestinal disease a public health problem in developing countries. The aim of this research is to know and measure the inhibition zone of ethanol extract of mahogany seed to the growth of Shigella dysenteriae bacteria with concentration 25%, 50%, 75%, 100%. This research is a descriptive study in vitro using disc method. The result of the research has found that the average inhibition zone diameter at 25%, 50%, 75%, 100% concentration is 6mm, 8mm, 8.33mm, 10.33mm. Negative controls do not provide inhibition and positive control yields an average diameter of 21.33 mm. Based on the above results, it can be concluded mahogany seed extract provides resistance to Shigella dysenteriae.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kamboja (Plumeria Sp) Terhadap Bakteri Escherichia coli Secara In Vitro

2017 ◽  
Vol 1 (1) ◽  
pp. 74
Author(s):  
Susanti Erikania ◽  
Yetty Hariningsih
Keyword(s):  
Escherichia Coli ◽  
Treatment Group ◽  
Streptococcus Mutans ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Nutrient Agar ◽  
Diffusion Method ◽  
Negative Controls

Tanaman obat menjadi sumber utama ditemukannya senyawa kimia baru yang memiliki khasiat secara farmakologi, salah satunya adalah tanaman kamboja (Plumeria sp). Tanaman kamboja adalah salah satu tanaman tradisional yang dilaporkan mempunyai berbagai khasiat, antara lain daunnya sebagai pencahar, antigatal dan antibakteri, buah dan kulit batangnya dilaporkan berefek antiinflamasi. Tujuan dari penelitian ini adalah  mengetahui aktivitas daun kamboja sebagai antibakteri terhadap Escherichia coli secara in vitro. Penelitian ini menggunakan metode difusi cakram dengan 5 kelompok perlakuan meliputi kontrol negatif yaitu DMSO dengan konsentrasi 10 %, kontrol positif yaitu kloramfenikol konsentrasi 30 µg/disk dan ekstrak etanol daun kamboja (Plumeria sp) masing-masing sebesar 25 %, 50 % dan 100 %. Media yang digunakan adalah media nutrient agar (NA). Media steril yang telah diinokulasi dengan Escherichia coli selanjutnya diberi perlakuan dengan kertas cakram yang terdiri dari kontrol negatif, kontrol positif dan sampel, kemudian diinkubasi pada suhu 37°C selama 24 jam. Selanjutnya dilakukan pengukuran zona hambat menggunakan jangka sorong. Penelitian menunjukkan adanya zona hambat pada kelompok perlakuan ekstrak etanol daun kamboja  pada konsentrasi 25%, 50%, 100 % dan kontrol positif, dan tidak tedapat zona hambat pada kelompok perlakuan dengan kontrol negatif. Ekstrak etanol daun kamboja mampu menghambat pertumbuhan Escherichia coli dengan daya hambat minimal adalah konsentrasi 25% yaitu menghasilkan zona hambat sebesar 7,13 mm, konsentrasi 50 % menghasilkan zona hambat sebesar 10,17 mm dan konsentrasi 100% memiliki nilai zona hambat paling besar yaitu 14,50 mm. Kata Kunci            : Ekstrak etanol daun kamboja, Metode difusi cakram,  Streptococcus mutans, Uji aktivitas antibakteri ABSTRACTMedicinal plants are the main source of the discovery of new chemical compounds that have pharmacological properties, one of which is frangipani (Plumeria sp). Frangipani plants are one of the traditional plants that are reported to have various benefits, including laxative, and antibacterial leaves, fruit and bark reported to have anti-inflammatory effects. The purpose of this study was to determine the activity of frangipani leaves as an antibacterial against Escherichia coli in vitro. This study uses a disk diffusion method with 5 treatment groups including negative control, namely DMSO with a concentration of 10%, positive control that is chloramphenicol concentration of 30 µg / disk and ethanol extract of frangipani leaves (Plumeria sp) each by 25%, 50% and 100% . The media used is nutrient agar (NA). Sterile media that have been inoculated with Escherichia coli are then treated with disc paper consisting of negative controls, positive controls and samples, then incubated at 37 ° C for 24 hours. Furthermore, inhibition zone measurements were made using calipers. The study showed that there were inhibitory zones in the treatment group of frangipani leaf ethanol extract at concentrations of 25%, 50%, 100% and positive control, and there were no inhibitory zones in the treatment group with negative controls. Ethanol extract of frangipani leaves can inhibit the growth of Escherichia coli with a minimum inhibitory concentration of 25% which produces a inhibition zone of 7.13 mm, a concentration of 50% produces an inhibitory zone of 10.17 mm and a concentration of 100% has the greatest inhibition zone value of 14 50 mm. Keywords: Frangipani leaf ethanol extract, Disc diffusion method, Streptococcus mutans, activity test antibacterial

scholarly journals UJI DAYA ANTIMIKROBA EKSTRAK ETANOL DAUN SIRIH MERAH (Piper Crocatum Ruiz & Pav.) TERHADAP PERTUMBUHAN Staphylococcus aureus ATCC 6538, Eschericia coli ATCC 11229 DAN Candida albicans ATCC 10231 SECARA IN VITRO

Biomedika ◽  
2011 ◽  
Vol 5 (1) ◽  
Author(s):  
Anika Candrasari ◽  
M. Amin Romas ◽  
Ovi Rizky Astuti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Betel Leaf

Red betel leaf contains flavonoids, alkaloids and essential oils that are suspected able to inhibit the growth of microbes. This study aims to analyze antimicrobial activity of ethanol extract of red betel leaf agains to Staphylococcus aureus, Eschericia coli and Candida albicans. Subjects were red betel leaf ethanol extract at concentration of 2.5%, 5%, 10%, 20%, 40%, 80% and 100%. As a positive control used amoxycilin, chloramphenicol and ketoconazole. The study showed that concentration 10 % - 100 % inhibit the growth of Staphylococcus aureus. In Escherichia coli all the data obtained, having an average which is not much different from the mean of the negative control. While on Candida albicans average diameter of inhibition zone of extract concentration 40% p = 0.197 concluded that statistically has significant antifungal power compared with ketokonazole.Keywords: Ethanol extract, Piper Ruiz & Pav crocatum, Antimicrobial, Staphylococcus aureus, Escherichia coli, Candida albican

scholarly journals Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

2020 ◽  
Vol 151 ◽  
pp. 01029
Author(s):  
Nuzul Asmilia ◽  
Mahdi Abrar ◽  
Yudha Fahrimal ◽  
Amalia Sutriana ◽  
Yobeswi Husna
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Hexane Extract ◽  
Clear Zone ◽  
Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

scholarly journals 25. The effect of Fingerroot Rhizome (Boesenbergia pandurata) Extract on the Growth of Staphylococcus aureus in Vitro

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Silvia P.N Keliat ◽  
Darniati Darniati ◽  
Abdul Harris ◽  
Erina Erina ◽  
Rinidar Rinidar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Final Conclusion ◽  
Distilled Water

This study aimed to determine the inhibitory effect and the concentration of fingerroot extract to inhibit Staphylococcus aureus growth. The data was analyzed descriptively. Ampicillin was used as positive control, distilled water was used as negative control, and the treatments were given fingerroot extract with a concentration of 5%, 15%, 25%, 35% and 45%. This study was conducted with three replications. The parameter measured was the diameter of inhibition zone formed by diffusion method. The average diameter of inhibition zone of the fingerroot extract were 15% : 10.3 mm; 25% : 13,6mm; 35% : 18,7mm; 45% ± 21,1mm, and at a concentration of 5% the inhibition zone is not formed. The final conclusion is that the fingerroot extract can inhibit the growth of Staphylococcus aureus. The higher concentration of fingerroot extract, the more extensive inhibition zone formed.

scholarly journals UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN SIRSAK (ANNONA MURICATA LINN.) TERHADAP BAKTERI PROPIONIBACTERIUM ACNES

2019 ◽  
Vol 6 (1) ◽  
pp. 65
Author(s):  
Yurlina Zai ◽  
Agnes Yohana Kristino ◽  
Sri Lestari Ramadhani Nasution ◽  
Oliviti Natali
Keyword(s):  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Research Model ◽  
Negative Controls

This research aims to determine the effectiveness of the antibacterial soursop leaf extract (Annona muricata L.). The method used is the agar diffusion method. The leaves were extracted by maceration method, using ethanol as a solvent. The soursop leaf extract obtained was then diluted using ethanol 96% at a concentration of 20%, 40%,60%, 80%. Positive control using clindamycin and negative controls using sterile aquadest. The research model used in this study is an experimental research model In Vitro of type. The results showed that soursop leaf extract had antibacterial effectiveness against Propionibacterium acnes with an average inhibition zone diameter of 9,7 mm; 13,7 mm; 15,7 mm; 16,3 mm; positive control (clindamysin) 21,0 mm; and negative control (aquadest) have no value tobe able to inhibit bacterial growth.

scholarly journals Effectiveness of Chinese Leaf Extract and Leaf Plants (Jatropha Multifida L.) As Antibacterial to Bacterial Growth Staphylococcus Aureus In Vitro

2018 ◽  
pp. 29-33
Author(s):  
Dwi Anggita ◽  
Yusriani Yusriani ◽  
Dian Amelia Abdi ◽  
Vivin Desiani
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
The Difference ◽  
Jatropha Multifida

Jatropha multifida L. contains alkaloids, tannins, flavonoids, saponins and phenolic acids that differ from each part of the plant and the content of these substances is what makes L. multifida Jatropha has a function as an antibacterial so that the leaf extract and the gap of chinese distance ( Jatropha multifida L.) allegedly capable of inhibiting the growth of Staphylococcus aureus bacteria. The aim of this research was to know the effectivity of leaf extract and gum of Jatropha multifida L. to the growth of Staphylococcus aureus bacteria in vitro.Penelitian used true experimental post test design. Using disc diffusion method with concentration 25; 50; 75; and 100% v / v leaf and gum china distance. Positive control was used Clindamycin 5μg drip antibiotic paper disc. The data were analyzed descriptively. The result of the research showed the difference of different inhibition zone between treatments. In chinese leaf extracts showed different mean diameters, at concentrations of 25%, 50%, 75% with inhibit zone formed respectively 0 mm, 9.32 mm, and 17.48 mm and the highest inhibition zone at concentration of 100% with an average diameter of 22.24 mm. In the gap of chinese distance showed a different mean diameter, at concentrations of 25%, 50%, 75% with the inhibit zone formed respectively 16.08 mm, 18.15 mm, and 18.63 mm and the highest inhibition zone at a concentration of 100% with an average diameter of 21.91 mm. Clindamycin positive controls show an average inhibit zone of 23.31 mm. It was concluded that there was an effect of leaf extract and resin of chinese distance plant (Jatropha multifida L.) on growth of Staphylococcus aureus bacteria, and the best concentration was 100% concentration and almost closer to the inhibitory zone of Clindamycin antibiotics as positive control.

scholarly journals EFFECT OF CURCUMA XANTHORRHIZA ROXB. ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS DUAL-SPECIES BIOFILMS

2019 ◽  
pp. 75-78
Author(s):  
FARIDA ERVINTARI ◽  
RIA PUSPITAWATI ◽  
SRI UTAMI
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Culture ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Streptococcus Sanguinis ◽  
Curcuma Xanthorrhiza ◽  
Ethanol Extracts ◽  
Negative Controls

Objective: This study aimed to determine the effect of ethanol Curcuma extract on the viability of S. mutans and Streptococcus sanguinis in a dualspeciesin vitro biofilm model.Methods: Dual-species biofilms of S. mutans and S. sanguinis were exposed to ethanol Curcuma extract at various concentrations. The sample ofsaliva was gathered from healthy volunteers. Chlorhexidine 0.2% was used as a positive control, and bacterial culture without intervention servedas a negative control. The total suspensions of 10−as were prepared for S. mutans and S. sanguinis cells. The bacteria were incubated for 20 h (activematuration phase) and 24 h (maturation phase).Results: The result showed decreased S. mutans and S. sanguinis viability after exposure to 0.2%–25% Curcuma ethanol extracts during the activeaccumulation and maturation phases. The decrease in bacterial viability was significantly different in all concentrations of Curcuma ethanol extractscompared with negative controls (p<0.05) in the active accumulation and maturation phases.Conclusion: Temulawak ethanol extract (starting at 0.2%) can decrease the viability of S. mutans and S. sanguinis in a dual species in vitro biofilmmodel during the accumulation and maturation phases.

POTENSI ANTIBAKTERI EKSTRAK ETANOL HERBA MENIRAN HIJAU (Phyllanthus niruri Linn.) DALAM MENGHAMBAT PERTUMBUHAN STAPHYLOCOCCUS AUREUS

2019 ◽  
pp. 144-150
Author(s):  
Vector Stephen Dewangga ◽  
Muhammad Taufiq Qurrohman
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Homogeneity Test ◽  
Phyllanthus Niruri ◽  
Kolmogorov Smirnov ◽  
Post Hoc ◽  
Negative Controls ◽  
Positive Controls

Penggunaan bahan alam semakin meningkat dan masih menjadi andalan di beberapa negara berkembang. Penelitian kali ini menggunakan ekstrak etanol dari herba Phyllanthus niruri Linn. Dalam usaha menghambat pertumbuhan Staphylococcus aureus. S. aureus merupakan salah satu mikroflora normal di wajah yang berperan dalam infeksi jerawat. Penelitian ini merupakan penelitian eksperimental menggunakan Rancangan Acak Lengkap dengan 7 perlakuan dan 5 ulangan. Perlakuan yang digunakan adalah pemberian ekstrak etanol herba P. niruri Linn. untuk konsentrasi 5%; 25%; 50%; 75%; 100%, pengenceran menggunakan pelarut DMSO 10%. Kontrol positif menggunakan ciprofloxacin, sedangkan kontrol negatif menggunakan DMSO 10%. Zona hambat yang diperoleh dianalisis menggunakan uji Kolmogorov–Smirnov dan uji homogenitas Levene. Apabila variansi data homogen, maka dilanjutkan analisis dengan Anova yang dilanjutkan dengan uji Post Hoc. Dari perhitungan kontrol negatif, variasi konsentrasi (5%, 25%, 50%, 75%, 100%) dan kontrol positif diperoleh rata-rata zona radikal berturut sebesar 6 mm; 7,46 mm; 7,52 mm; 7,6 mm; 8,52 mm; 8,98 mm; 31,08 mm. Semua variasi konsentrasi ekstrak etanol herba P. niruri Linn. mampu menghasilkan zona hambat radikal dengan zona hambat terbesar tetap dijumpai pada kontrol positif. Hal ini menunjukkan bahwa konsentrasi ekstrak etanol herba P. niruri Linn. yang semakin besar akan berbanding lurus dengan peningkatan aktivitas penghambatan, namun belum seoptimum kontrol positif.   The use of natural materials is increasing and is still a mainstay in several developing countries. This research was used ethanol extract from herb Phyllanthus niruri Linn. in an effort to inhibit the growth of Staphylococcus aureus. S. aureus is one of the normal microflora on the face that helps infection. This research is an experimental study used a completely randomized design with 7 preparations and 5 replications. The treatment used ethanol extract of P. niruri Linn herb. for a concentration of 5%; 25%; 50%; 75%; 100%, the dilution used 10% DMSO solvent. Positive controls used ciprofloxacin, while negative controls used DMSO 10%. The inhibition zone obtained was analyzed using the Kolmogorov-Smirnov test and the Levene homogeneity test. If the data variance is homogeneous, then the analysis is continued with ANOVA followed by the Post Hoc test. From the calculation of negative controls, various concentration (5%, 25%, 50%, 75%, 100%) and positive controls obtained an average radical zone of 6 mm; 7.46 mm; 7.52 mm; 7.6 mm; 8.52 mm; 8.98 mm; 31.08 mm. All variations in the concentration of ethanol extract of P. niruri Linn. herbs able to produce a radical inhibition zone with the largest inhibition zone still found in positive controls. This showed that the greater concentration of ethanol extract of P. niruri Linn. herb. will be directly proportional to the increase in inhibition activity, but not optimal as much as positive control.

UJI DAYA HAMBAT EKSTRAK ETANOL RIMPANG TEMU GIRING (CURCUMA HEY NEANA VAL.) TERHADAP PERTUMBUHAN ESCHERICHIA COLI SECARA IN VITRO

2017 ◽  
Vol 1 (1) ◽  
pp. 68
Author(s):  
Aditya Maulana Perdana Putra ◽  
Rustifah Rustifah ◽  
Muhammad Arsyad
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Volatile Oil ◽  
Negative Control ◽  
Diffusion Method ◽  
Minimum Inhibitory Concentrations ◽  
Escherchia Coli

Infection is a major problem that the world’s attention. Infectious diseases have caused the death of over 13 million people worldwide every year, particularly in the developing countries such as Indonesia. One of the species of bacteria that cause infections are Escherichia coli. Curcuma heyneana rhizome (Curcuma heyneana Val.) is a plant that is often used as a traditional medicine to antibacterial. This study was intended to determine the antibacterial inhibition activity, and minimum inhibitory concentrations (MIC) from the usage of an ethanol extract in the curcuma heyneana rhizome (Curcuma heyneana Val.) for the growth of an Escherchia coli by in vitro.Curcuma heyneana rhizome (Curcuma heyneana Val.) was extracted using maseration method with solvent ethanol. Each extracts identified the active compounds group consisting of flavonoids, saponins, curcumin, volatile oil, and tannins. This study was an experimental laboratoric by using the diffusion method with discs blank with 10  treatment groups concentration which are 100%, 50%, 25%, 12,5%, 6,25%, 3,12%, 1,56%, 0,78%, positive control and negative control with three repetitions.The phytochemical screenings analysis showed curcuma heyneana rhizome (Curcuma heyneana Val.) of ethanol extract containing flavonoids, saponins, curcumin, volatile oil, and tannins. Ethanol extract in the curcuma heyneana rhizome (Curcuma heyneana Val.) had an effectiveness in inhibiting the growth of an Escherchia coli by in vitro. Minimum inhibitory concentrations (MIC) from the usage of an ethanol extract in the curcuma heyneana rhizome (Curcuma heyneana Val.) for the growth of an Escherchia coli by in vitro is 12,5% with average inhibition zone 9,77 mm. The results showed that the ethanol extract curcuma heyneana rhizome (Curcuma heyneana Val.) had an effectiveness in inhibiting the growth of an Escherchia coli, it showed inhibition zone diameter due to higher concentrations of the resistance is increasing. Analysis of the test data with Kruskal-Wallis (sig) = 0,001 which is smaller than α < 0,05. It means that there are significant differences in the average diameter of an each concentration of ethanol extract of curcuma heyneana rhizome in i

scholarly journals Aktivitas Antibakteri Ekstrak Etanol Daun Sukun (Artocarpus altilis) Terhadap Bakteri Staphylococcus aureus Dan Escherichia coli

2020 ◽  
pp. 10-20
Author(s):  
Fuan Maharani Fiana ◽  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Ery Purwanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Artocarpus Altilis ◽  
Escherichia Coli Bacteria

Diarrhea is caused by bacterial infections, especially Staphylococcus aureus and Escherichia coli. The breadfruit leaves (Artocarpus altilis) are known contain flavonoid compounds, alkaloids, saponins, and tannins which can be used as antibacterial. This research was conducted to determine the ethanol extract of the breadfruit leaves against Staphylococcus aureus and Escherichia coli bacteria. The manufacture of the breadfruit leaf extract was using a maceration method with 96% ethanol solvent. The result of the tube test and the TLC test was positive, the breadfruit leaf extract containing flavonoids and tannins which have potential as antibacterial. The antibacterial test was carried out using the disc diffusion method (Kirby-Bauer test) against Staphylococcus aureus and Escherichia coli bacteria incubated for 1 x 24 hours. The extract concentrations tested were 10%, 15% and 20%. The positive control was using erythromycin antibiotics and the negative control was using aquades. The identification of the chemical content of plants is done by tube test and the TLC test. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Staphylococcus aureus bacteria, the average diameter of inhibitory zones respectively were 3.67, 3.50 and 2.67 mm with the positive control diameter of inhibition zone was 18.5 mm, the negative control diameter of inhibition zone was 0 mm. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Escherichia coli bacteria, the average diameter of inhibitory zones respectively were 5.33, 3.17 and 3.33 mm with the positive control inhibition zone diameter of 28.5 mm and the negative control of inhibition zone diameter of 0 mm. The activity of ethanol extract of the breadfruit leaves against the Staphylococcus aureus and Escherichia coli bacteria is included in the weak category.

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