scholarly journals Antimicrobial Effect Of Honey Distributed Around Pasar Minggu Jakarta Selatan

2017 ◽  
Vol 8 (2) ◽  
pp. 101-106
Author(s):  
Ruth Elenora Kristanty ◽  
Junie Suriawati ◽  
Priyanto Dwi Nugroho
Keyword(s):  
Infectious Diseases ◽  
Food Product ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Bacteria Growth ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Almost All

Honey is a highly nutritious food product and consumed by almost all the population in the world. It has a function as an antimicrobial. Staphylococcus aureus (S. aureus) is a common Gram-positive bacteria in food and Escherichia coli (E. coli) is a Gram-negative bacteria that often appears in environmental sanitation issues that both can cause infectious diseases.  Some infectious diseases can be treated with antimicrobials such as honey. The purpose of this study was to test the antimicrobial effects on honey products distributed in Pasar Minggu area. The antimicrobial effect test was performed in vitro using agar diffusion method by measuring the inhibition zone formed where the bacteria growth was inhibited by the presence of sample. The concentration of samples were 25%, 50%, 75%, and 100% (not diluted) and as aquades control. The results showed that honey tested with various dilution concentrations resulted inhibition zone and. The higher concentration of the inhibited zone zone showed antimicrobial activity against S. aureus and E. coli.

scholarly journals Evaluation of the Potential Nephroprotective and Antimicrobial Effect ofCamellia sinensisLeaves versusHibiscus sabdariffa(In VivoandIn VitroStudies)

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Doa’a Anwar Ibrahim ◽  
Rowida Noman Albadani
Keyword(s):  
Antibacterial Activity ◽  
Green Tea ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Health State ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Different Sources

Green tea and hibiscus are widely consumed as traditional beverages in Yemen and some regional countries. They are relatively cheap and the belief is that they improve health state and cure many diseases. The aim of this study was to evaluate the potential protective and antibacterial activity of these two famous plantsin vitrothrough measuring their antibacterial activity andin vivothrough measuring nonenzymatic kidney markers dysfunction after induction of nephrotoxicity by gentamicin. Gram positive bacteria like MRSA (methicillin resistantStaphylococcus aureus) were isolated from hospitalized patients’ different sources (pus and wound) and Gram negative bacteria includingE. coliandP. aeruginosawere usedin vitrostudy. In addition, the efficacy of these plants was assessedin vivothrough measuring nonenzymatic kidney markers including S. creatinine and S. urea. Green tea was shown antimicrobial activity against MRSA with inhibition zone 19.67 ± 0.33 mm and MIC 1.25 ± 0.00 mg/mL compared with standard reference (vancomycin) 18.00 ± 0.00 mg/mL. Hibiscus did not exhibit a similar effect. Both Hibiscus- and green tea-treated groups had nephroprotective effects as they reduced the elevation in nonenzymatic kidney markers. We conclude that green tea has dual effects: antimicrobial and nephroprotective.

PHYTOCHEMICAL INVESTIGATION AND ANTIMICROBIAL PROPERTIES OF DIOSCOREA BULBIFERA TUBER

2017 ◽  
Vol 10 (12) ◽  
pp. 317
Author(s):  
Dahiya P
Keyword(s):  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Investigation ◽  
Dioscorea Bulbifera ◽  
Acinetobacter Sp ◽  
Almost All

  Objective: The inhibitory properties of successive extracts from Dioscorea bulbifera (Dioscoreaceae) tubers have been evaluated for the presence of phytochemical constituents and antimicrobial efficacy against multidrug-resistant (MDR) clinical isolates was evaluated.Methods: The tuber of D. bulbifera was oven dried and extracted successively with n-hexane, chloroform, methanol, ethanol, and water. The antimicrobial potential of successive extracts against MDR isolates was studied by agar well-diffusion method. Qualitative phytochemical analysis was performed.Results: Qualitative phytochemical analysis demonstrated the presence of steroids, flavonoids, cardiac glycosides, saponins, and reducing sugars in almost all the extracts tested. Anthraquinones, phlobatanins, and tannins were not reported in any extracts tested. The in vitro antimicrobial activity of various solvents and water extracts of D. bulbifera was further investigated against ten MDR bacteria and three fungi, respectively. Aqueous and chloroform extracts were found to be more potent being capable of exerting significant inhibitory activities against the majority of the isolates such as Escherichia coli, Acinetobacter sp., Salmonella paratyphi, Klebsiella pneumoniae, and Candida albicans. The highest inhibitory activity was observed for K. pneumoniae with wide inhibition zone diameters (17 ± 0.15 mm), followed by E. coli 1(13 ± 0.11) mm, and Acinetobacter sp. (11 ± 0.12).Conclusion: Based on the present study, the extracts of D. bulbifera tubers have shown excellent activity against MDR microbial cultures tested. Further study is recommended for clinical evaluation, of the efficacy of crude extract in herbal medicine that can serve as a base for the development of novel potent drugs and phytomedicines.

scholarly journals Quantitative Phytochemical Analysis and Antimicrobial Potential of the Ethanol and Aqueous Extracts of the Leaf, Stem and Root of Chromolaena odorata (Asteraceae)

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Ugwoke C E C ◽  
Orji J. ◽  
Anze S P G ◽  
Ilodibia C V
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Infectious Diseases ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Chromolaena Odorata

Background: Plants contain secondary metabolites or phytochemicals, which when consumed by humans give therapeutic effect. This study therefore analyzed the phytochemical composition of Chromolaena odorata so as to give an idea of its possible pharmacological potentials. An antimicrobial assay was also carried out to verify claims on its use in the treatment of infectious diseases. Methods: The experimental procedure involved collection of the leaf, stem and root of the plant from the wild, authenticating the samples and drying under shade to facilitate pulverization. Preliminary qualitative and quantitative phytochemical analyses were done using standard methods to reveal the presence and percentage composition of basic phytochemicals. The powder was also macerated in ethanol and water to produce ethanol and aqueous crude extracts that were reconstituted in normal saline to concentrations (mg/ml) of 150, 100, 75 and 50. Clinical isolates of Staphylococcus aureus, Salmonella typhi, Escherichia coli, Aspergillus niger and Candida albicans were screened for sensitivity to the extracts using the agar well diffusion method. Results: The plant parts contain alkaloids, tannins, flavonoids, saponins, terpenoids, phenols, cardiac glycosides, and sterols at varying concentrations. The leaf however had highest concentration of almost all phytochemicals present. The antimicrobial activity of the plant was concentrationdependent in all parts of the plant and both extracts. Aspergillus niger and Candida albicans were most susceptible while Salmonella typhi and Escherichia coli were least susceptible with an average Inhibition Zone Diameter (IZD) of about 8 – 10 mm. Conclusion: The substantial quantity of the basic phytochemicals in Chromolaena odorata could render it a utility plant in therapeutic use. Due to the profound antimicrobial effect as revealed by the IZDs, the plant could be classified as a broad spectrum antimicrobial agent. Thus, the claim on its potency in treatment of infectious diseases by traditional medical practitioners could be said to be justified.

scholarly journals Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

2020 ◽  
Vol 7 (1) ◽  
pp. 26-32
Author(s):  
Bendella Amina nor elhouda ◽  
Ghazi Kheira ◽  
Meliani Samia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Bovine Mastitis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition

AbstractThe aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

scholarly journals Synthesis and Pharmacological Evaluation of 2-(4-(3 (Substituted Phenyl) Acryloyl) Phenoxy)-N, N Diphenylacetamides

2020 ◽  
Vol 10 (5) ◽  
pp. 274-292
Author(s):  
Rohit Kumar ◽  
Sushil Kumar ◽  
Mohammad Asif Khan
Keyword(s):  
Antibacterial Activity ◽  
Schiff Bases ◽  
Biological Activities ◽  
Diffusion Method ◽  
Gram Positive ◽  
Standard Drug ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Pharmacological Evaluation

Recently a series of Schiff bases of diphenylamine derivatives have been synthesized and evaluated in vitro for their antibacterial activity against pathogenic both Gram-positive bacteria B. subtitles and Gram-negative bacteria E. coli using ciprofloxacin as standard drug at conc. of 50 μg/ml and 100 μg/ml. Literature review revels that chalcones possesses various biological activities like antimicrobial, antiviral, anti-inflammatory, anticancer and sedative etc. Therefore the present study was designed on synthesis and pharmacological evaluation of 2-(4-(3 (Substituted Phenyl) Acryloyl) Phenoxy)-N, N Diphenylacetamides. Target compound was synthesized by reaction of chloroacetylchloride with diphenylamine to afford 2-chloro-N, N-diphenylacetamide which further by reaction with substituted Chalcones and characterized following recrystallization and evaluated for anti-microbial potential through cup-diffusion method. In results, the target compounds were tested for activity against B. Subtilis, E.Coli and C. albicans. The chalcones having the lipophilic 4-chloro group (RKCT2) showed the greatest antimicrobial activity (zone of inhibition 20 & 22 mm against. B. subtilis, E. Coli, C. Albicans respectively. It suggests further researchers to go through anti-microbial evaluations against a more varieties of bacteria and fungi. Keywords: Schiff bases of diphenylamine derivatives, antibacterial activity, Gram-positive bacteria, 2-(4-(3 (Substituted Phenyl) Acryloyl) Phenoxy)-N, N Diphenylacetamides

scholarly journals Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro

2021 ◽  
Vol 10 (1) ◽  
pp. 16
Author(s):  
Saskia Arientika Wahyuningrum ◽  
Meiskha Bahar ◽  
Andri Pramesyanti Pramono
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
P Value ◽  
Gram Positive Bacteria ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
The One ◽  
One Way Anova

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa

scholarly journals Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

2021 ◽  
Vol 21 (1) ◽  
pp. 281
Author(s):  
Muhammad Zaidan Tsani Ariandi ◽  
Meiskha Bahar ◽  
Hany Yusmaini ◽  
Fajriati Zulfa ◽  
Cut Fauziah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
P Value ◽  
E Coli ◽  
Botanical Gardens

Actinomycetes are found in soils with loose, humus, dry characteristics and around plant roots. Actinomycetes produce secondary metabolite compounds as antibacterial. Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi are Gram negative bacteria that can cause infection in humans. This study aims to determine the ability of the Actinomycetes metabolite filtrate from Bogor Botanical Gardens as an antibacterial agent against the growth of E. coli, P.aeruginosa and S. typhi in vitro. This study used an experimental design with samples of Actinomycetes isolats originating from the Bogor Botanical Gardens soil using the disc diffusion method on Muller Hinton Agar (MHA) media by looking at the clear zone of bacterial growth around the disc paper. Of the three concentration groups, namely 50%, 60%, and 70%, the largest average inhibition zone is found at a concentration of 70% with the average for the three test bacteria E. coli, P.aeruginosa and S. typhi respectively: 4.23 mm; 3.0 mm and 8.43 mm. The results of the Kruskal - Wallis test with p value = 0.01 showed that there was an effect of the Actinomycetes metabolite filtrate on the growth of the three tested bacteria as antibacterials.  

scholarly journals IN VITRO EVALUATION OF ANTIBACTERIAL PROPERTY OF CATHARANTHUS ROSEUS (LINN.) G. DON. VAR. “ROSEA” AND “ALBA”

2018 ◽  
Vol 10 (5) ◽  
pp. 55 ◽  
Author(s):  
Amresh Kumar Yadav ◽  
Sanjeev Kumar Ambasta ◽  
Surendra Kumar Prasad ◽  
M. P. Trivedi
Keyword(s):  
Catharanthus Roseus ◽  
Pathogenic Bacteria ◽  
Antibacterial Property ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Life Saving

Objective: To evaluate the antibacterial property of crude, aqueous and organic solvent extract from leaf, stem and root parts of two different var. of Catharanthus roseus (i.e. “rosea” and “alba”) under in vitro conditions on various human pathogenic bacteria.Methods: Antibacterial activity of crude (fresh), aqueous, ethanolic, methanolic and equimolar (1:1) mixture of ethanolic dried leaf extract of variety “rosea” and “alba” was evaluated against various pathogenic bacteria viz. Bacillus subtilis, Escherichia coli and Staphylococcus aureus by disk diffusion method under in vitro conditions.Results: Gram-positive bacteria were found to be more susceptible than Gram-negative. Dried extracts of root, stem and leaf of C. roseus var. “rosea” and “alba” plants showed maximum antibacterial potency against all the test microorganisms. The equimolar mixture of ethanolic dried leaf extracts of species “rosea” and “alba” exhibited the maximum zone of inhibition against B. subtilis, E. coli and S. aureus as compare to extract prepared from individual parts. The findings of the ethanolic mixture of dried leaves of the two varieties on the tested bactera confirm that the effect is potentiating which may be synergistic or additive.Conclusion: From the findings, it could be inferred that C. roseus var. “rosea” and “alba” could be efficiently used in the development of new life-saving drugs against bacterial pathogens. 

Antimicrobial activity of Saponins rich fraction of Cassia auriculata Linn against various microbial strains

2013 ◽  
Vol 2 (4) ◽  
pp. 85-87
Author(s):  
Supriya Deshpande ◽  
Shailesh Kewatkar ◽  
Vivek Paithankar
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Antimicrobial Effect ◽  
Pharmaceutical Journal ◽  
Diffusion Method ◽  
E Coli ◽  
Cassia Auriculata ◽  
Aeromonas Hydrophilia ◽  
Microbial Strains

In the present investigation, the saponins rich fraction of roots of Cassia auriculata L. was evaluated for antimicrobial activity against P. vesicularis, Streptococcus faecalis, Aeromonas hydrophilia, Salmonella typhae, Staphylococcus cohni, Serratia ficaria and E. coli at concentration of 12.5 mg/ml, 25 mg/ml, 37.5 mg/ml and 50 mg/ml. Antimicrobial activity of Cassia auriculata L. was carried out by well diffusion method. At maximum conc. i.e. 50mg/ml, antimicrobial effect of Saponin rich extract can be arranged in sequence of - P. vesicularis > Serratia ficaria > Streptococcus cohni > Aeromonas hydrophilic> Salmonella typhae > Sterptococcus faecalis > E. coli. The results indicate the saponins rich fraction of roots of Cassia auriculata L. might be exploited as natural drug for the treatment of several infectious diseases caused by these organisms. Cassia auriculata L. was observed to have antibacterial activity and can be used for medicinal purposes.DOI: http://dx.doi.org/10.3329/icpj.v2i4.14056 International Current Pharmaceutical Journal, March 2013, 2(4): 85-87 

scholarly journals Antibacterial Activity and Chemical Composition of Red Peacock Flower (Caesalpinia pulcherrima L.) Leaf Essential Oil

2019 ◽  
Vol 22 (6) ◽  
pp. 269-274
Author(s):  
Novena Risnalani Rintank Constani ◽  
Hartati Soetjipto ◽  
Sri Hartini
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Leaf Essential Oil

Peacock flower (Caesalpinia pulcherrima L.) leaves contain essential oils which can be used as an ingredient in cosmetics, perfume, aromatherapy, medicine, and supplements. The study was conducted to obtain essential oils from peacock flower leaves and determine the antibacterial activity against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa). Antibacterial activity test was carried out by the agar diffusion method, using paper discs. Measurements were made for the inhibition zone diameter (IZD) that appeared, while the essential oil component was analyzed using GC-MS. The results showed that the peacock flower leaves (C. pulcherrima) had a moderate to strong antibacterial effect at a concentration of 7.5%-20% against gram-positive bacteria (B. subtilis and S. aureus) and gram-negative bacteria (E. coli and P. aeruginosa). Gram-negative E. coli bacteria are relatively more sensitive to peacock flower leaf essential oil compared to other test bacteria. Peacock flower (C. pulcherrima) leaf essential oil is composed of 7 main components namely β-Cubebene 33.87%; Caryophyllene 23.00%; γ-Elemene 13.18%; α-Pinene 10.96%; Cadina-1(10),4-diene 10.20%; Copaene; 7.09%; β-Pinene 1.70%.

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